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1.
Induction of ammonium assimilation: leguminous roots compared with nodules using a split root system 总被引:1,自引:0,他引:1
Paul H. S. Reynolds Michael J. Boland Graeme S. McNaughton Raymond D. More William T. Jones 《Physiologia plantarum》1990,79(2):359-367
A split root system for nitrogen uptake, in which one part of the root system was exposed to nitrogen-free nutrient and the other to circulated buffered ammonium, was used to investigate the effects of ammonium per se on the enzyme pathway for its assimilation in nodules and roots of leguminous plants. Plants of Trifolium repens L. cv. Grasslands Huia grown in the system showed similar growth and similar free amino acid content in the NH+ 4 -fed roots and in nodulated plants. Studies of ammonium assimilation using [13 N]-NH+ 4 , applied to Glycine max [L.] Merr. cv. Amsoy plants, showed the label to be assimilated into amino acids in the NH+ 4 -fed roots and to be transported to the tops before subsequently appearing in the minus-N side of the split root system. Analysis of the xylem sap showed [13 N]-asparagine to be the principal labelled amino acid component. In these plants, levels of both allantoate and the nodule-specific isoenzyme aspartate aminotransferase-P2 were at least 10 times higher in the NH+ 4 -fed roots than in the minus-N side of the split root system. These studies strongly suggest that a nodule-type of ammonium assimilation was occurring in the NH+ 4 -fed side of the split root, and that this part of the root was transporting assimilatory products to the tops of the plants in a fashion analogous to that of a nitrogen-fixing nodule. These data implicate the involvement of NH+ 4 in the induction of its own assimilatory pathway. 相似文献
2.
S. Abarzua R. Altenburger R. Callies L.-H. Grimme A. Mayer D. Leibfritz U. Schiewer 《Physiologia plantarum》1993,89(3):659-663
Over a period of several days, rhythmic changes in extracellular NH+ 4 concentration take place in cultures of the cyanobacterium Microcystis firma (Bré et Lenorm.) Schmidle, strain Gromov/St. Petersb. 398, under conditions of restricted CO2 supply and light/dark alternation. The changes are enhanced by nitrate supply. Among the various processes generating intracellular NH+ 4 (NH4 4 uptake, NO− 3 reduction, protein and amino acid degradation, photorespiration), NO− 3 reduction appears as the one most important. This can be concluded from experiments with and without nitrate and/or ammonium in the medium. In the presence of saturating CO2 , continuous light, or continuous darkness, rhythmic NH+4 4 oscillations are not induced. Studies of the incorporation of NH+ 4 nitrogen by in vivo 15 N-NMR show that if CO2 is supplied, 15 N is accumulated in several components with the following time course: in the first hour in Gln (δ), in the second hour in the α-amino groups of most nonbranched amino acids, in the third hour in γ-aminobutyric acid (GABA), Orn (δ) and Lys (ε), and in the sixth hour in Ala. Carbon limitation, however, results in accumulation of label in the amide nitrogen of glutamine only. 相似文献
3.
Andrés P. Sanz Conrado Moreno-Vivián José M. Maldonado Agustín González-Fontes 《Physiologia plantarum》1995,95(1):39-44
The effect of the nitrogen source on carbohydrate and protein contents and on several enzymatic activities involved in the carbon and nitrogen metabolism was studied in Anabaena variabilis ATCC 29413 cells grown under a constant supply of either N, NO− 3 or NH+ 4 at different concentrations. An enhancement of protein content accompanied by a parallel decrease of carbohydrates was observed with increasing NO− 3 or NH+ 4 concentrations in the medium. In cultures containing 0.1 m M NO− 3 or 0.1 m M NH+ 4 nitrogenase (EC 1.18.6.1) activity was 74 and 66%, respectively, of that found in N2 -grown cells. This activity was still present with 1 m M NO− 3 or 1 m M NH+ 4 in the medium and even with 10 m M NO− 3 , but it was completely inhibited by 5 m M NH+ 4 . Ferredoxin-nitrate reductase (EC 1.7.7.2) activity was detected only in NO− 3 grown cells and simultaneously with nitrogenase activity. Increasing concentrations of combined nitrogen in the medium, especially NH+ 4 , promoted a concomitant decline of glutamine synthetase (EC 6.3.1.2), NADP+ -isocitrate dehydrogenase (EC 1.1.1.42), and NAD+ -malate dehydrogenase (EC 1.1.1.37) activities, suggesting that these enzymes play an important role in the regulation of carbon-nitrogen metabolism in cyanobacteria. 相似文献
4.
Alteration of N nutrition in Myrica gale induces changes in nodule growth, nodule activity and amino acid composition 总被引:1,自引:0,他引:1
Changes in nodule growth and activity and in the concentrations of soluble N compounds in nodules, leaves and xylem sap under conditions of altered N nutrition in the actinorhizal plant Myrica gale L. are reported. Altering the N nutrition of symbiotic plants may alter the internal regulation of combined N which in turn may regulate nodule growth and activity. Flushing nodules daily with 100% O2 caused a decline in amide concentration and an increase in nodule growth although plants had recovered some nitrogenase activity within 4 h of exposure to O2 . Samples of nodules, leaves and xylem sap were derivatized and amino acids identified and quantified using either reverse phase high performance liquid chromatography or gas chromatography-mass spectrometry in single ion monitoring mode. The ratio of asparagine in the nodules to that in the xylem was much higher in plants fed N (6.7 for NH+ 4 -fed and 8.3 for NO− 3 -fed plants) than for N2 -fixing plants (2.5). Significant amounts of 15 N added as 15 NH+ 4 or 15 NO− 3 accumulated in nodules following accumulation in the shoot which is consistent with the translocation of N to the nodules via the phloem. The uptake of 15 NH+ 4 led to the synthesis and subsequent translocation of glutamine in the xylem sap. These results are discussed in terms of the feedback mechanisms that may regulate nitrogen fixation in Myrica root nodules. 相似文献
5.
The uptake of the auxin type herbicide 2,4-D into rice seedlings ( Oryza sativa L. cv. Dunghan Shali) and its effects on the K+ , NH+ 4 and NO3 ion uptake and the K+ content were investigated at different pH values. A short incubation of the roots in 0.01 m M 2,4-D caused a marked ion uptake inhibition only at low pH. The non-auxin type herbicide benthiocarb did not produce such an inhibitory effect. Lowering of the pH in the external medium led to an increased 2,4-D uptake by the roots. These results can be explained by the increased H+ permeability of the membranes, allowing a more rapid entrance of 2,4-D into the root cells, thereby inhibiting the active ion uptake. Rice roots not subjected to 2,4-D treatment responded to H+ stress with an increased anomalous K+ uptake and a decreased K+ content. With reference to the effects of pH changes on the ion and 2,4-D uptake, possible transport mechanism of NH+ 4 and 2,4-D are briefly discussed. 相似文献
6.
Nitrite in the root zone and its effects on ion uptake and growth of wheat seedlings 总被引:1,自引:0,他引:1
The immediate and posteffects of various concentrations of NaNO2 on ion uptake of wheat ( Triticum aestivum L. cv. GK Öthalom) seedlings were studied at different pH values. Without pretreatment, the higher the concentration of NaNO2 the greater was the decrease in uptake of K+ into the roots, both at pH 4 and pH 6. At pH 6 but not at pH 4 the reverse was true when the seedlings were pretreated with NaNO2 . Due to the high Na+ content of the roots, an effect of Na+ in this process cannot be excluded. Nitrite was taken up by the roots more rapidly than nitrate. Nitrite at 0.1 m M in the medium induced the development of an uptake system for both NO− 2 and NO− 3 in wheat roots. At higher concentrations pretreatment with NO− 2 decreased NO− 3 uptake by the roots, but NO3 did not inhibit the uptake of NO2 . The toxic effect of NO− 2 was strongly pH dependent. Lower pH of the external solution led to an increased inhibition by NO− 2 of both ion uptake and growth of seedlings. The inhibitory effect of NO− 2 differed considerably for roots and shoots. The roots and especially the root hairs were particularly sensitive to NO− 2 treatment. 相似文献
7.
The use of compound continuous flow diffusion chemostats to study the interaction between nitrifying and nitrate-reducing bacteria 总被引:1,自引:0,他引:1
Abstract The interactions occuring between populations of a nitrate-respiring Vibrio sp. and autotrophic nitrifying bacteria belonging to the genera Nitrosomonas and Nitrobacter have been investigated in a compound bi-directional flow diffusion chemostat at a dilution rate of 0.025 h−1 and a temperature of 25°C. When grown under NO− 3 limitation, the Vibrio sp. produced NH+ 4 as the principal end-product of nitrate respiration, and there was a corresponding significant increase in cell numbers of the Nitrosomonas sp. population, which derived energy by the oxidation of NH+ 4 to NO− 2 . Nitrite in turn was used by the Nitrobacter sp. population as an energy source with the concomitant regeneration of NO− 3 . Under NO− 3 excess growth conditions the Vibrio sp. produced NO− 2 rather than NH+ 4 as the major product of NO− 3 dissimilation, and growth of the Nitrobacter population was stimulated as increased quantities of NO− 2 became available. In contrast, the Nitrosomonas sp. population declined sharply as the energy source NH+ 4 became limiting. These data demonstrate that defined mixed populations of obligately aerobic nitrifying bacteria and facultatively anaerobic nitrate respiring bacteria can co-exist for extended time periods and operate an internal nitrogen cycle which is energetically beneficial to both populations. 相似文献
8.
Photosynthetic CO2 -fixation, chlorophyll content, growth rate and nitrate reductase activity were used to examine the influence of NH+ 4 -N and NO− 3 -N on Sphagnum magellanicum cultivated under defined conditions in phytotrons. NO− 3 -concentrations up to 322 μ M were found to be favourable. Increased NH+ 4 concentrations, however, resulted in growth inhibition and decreased chlorophyll content at concentrations ≧ 255 μ M ; e.g. 600 μ M NH+ 4 caused a 20% reduction of nitrate reductase activity and net photosynthesis. For raised bog Sphagna an improved standard nutrient solution is proposed with the following ion concentrations (μ M ): 55 Na+ ; 17 K+ ; 95 NH+ 4 ; 22 Ca2+ ; 22 Mg2+ ; 2 Fe3+ ; 20 Cl− ; 100 NO− 3 ; 57 SO2- 4 ; 7.4 H2 PO− 4 ; trace elements: A-Z solution (Hoagland) 50 μl 1000 ml−1 ; pH 5.8. 相似文献
9.
Abstract The kinetic parameters of NH+ 4 -uptake in yeast cells were determined by a method that is based on the following changes in the external NH+ 4 concentration in cell suspensions by using NADH-dependent glutamate formation from NH+ 4 and 2-oxoglutarate. The kinetics of the observed NADH oxidation were analyzed by computer and enabled an estimation of V max and K m of the NH+ 4 -uptake system of the cells. 相似文献
10.
C.E. DEANE-DRUMMOND 《Plant, cell & environment》1985,8(2):105-110
Abstract. Net NO3 uptake by NO− 3 deficient Chara cells was used to calculate [NO− 3 ]c assuming that the cytoplasm occupies 10% total volume and that nitrate reduction and storage are negligible (i.e. maximum [NO− 3 ]c was calculated). A linear relationship was found between NO− 3 efflux and [NO− 3 ]c . There was an initial burst of NO− 3 efflux when NH+ 4 was added, followed by a slower efflux rate which matched influx rate such that net NO− 3 uptake was zero. Over 50% of NO− 3 that had been taken up in 2 h was lost within the first 5 min of NH+ 4 addition. The Nernst equation was used to predict the direction of the electrochemical driving force for NO− 3 entry. Under the experimental conditions used NO− 3 efflux is actively transported. The differential involvement of both NO− 3 influx and NO− 3 efflux in the regulation of NO− 3 uptake is discussed and a model is proposed to account for these results which envisages discrete NO− 3 influx and NO− 3 efflux carriers. 相似文献
11.
Relation of light and nitrogen source to growth, nitrate reductase and glutamine synthetase activity of jack pine seedlings 总被引:5,自引:0,他引:5
Two-month-old jack pine ( Pinus banksiana Lamb.) seedlings were placed in a greenhouse where both nitrogen source and light level were varied. After 4 months, whole seedling biomass, leaf biomass and relative growth rate were greatest in seedlings grown with NH+ 4 /NO/NO− 3 -N and full light (FL) and least in seedlings grown with NO − 3 -N and low light (LL). NO − 3 -seedlings grown under full light and NH+ 4 /NO− 3 -seedlings grown under low light were approximately equal. This indicates that the extra carbon costs of assimilating only NO− 3 -N were similar to the reduction of carbon fixation resulting from a 50% decrease in photon flux density. Percentage and total nitrogen content of needles were greater in seedlings grown under low light independent of nitrogen fertilization. Percentage and total nitrogen content of roots were higher under low light and lower when fertilized with NO− 3 .
Nitrate reductase (NR) activity was higher in roots than in needles, while glutamine synthetase (GS) activity was higher in needles than in roots. Low light resulted in decreased NR activity (mg N)−1 in needles, but not in roots. However, no nitrate was detected in the needles in any treatment. GS activity, on the other hand, was greater under low light in both needles and roots. GS activity in needles is most likely involved with the reassimilation rather than the initial assimilation of ammonium. Some implications of these shifts in enzymatic activity for ecological phenomena in forests are discussed. 相似文献
Nitrate reductase (NR) activity was higher in roots than in needles, while glutamine synthetase (GS) activity was higher in needles than in roots. Low light resulted in decreased NR activity (mg N)
12.
Ammonium is known to inhibit nitrogenase activity, but at low concentrations it may support nitrogenase activity. This work describes the effect of different concentrations of NH+ 4 as the N-source for growth and particularly for nitrogenase-based production of hydrogen from malate, butyrate and lactate. Two different Rho-dopseudomonas strains (ATCC 23782 and ST 407) were tested. Best growth was observed in the lactate-NH+ 4 media. Photoproduction of H2 for cells grown with low levels (3.8 mmol/1) of NH+ 4 equalled that of cells grown with glutamate as N-source. 相似文献
13.
Uptake regions of inorganic nitrogen in roots of carob seedlings 总被引:2,自引:0,他引:2
Three-week-old seedlings of carob ( Ceratonia siliqua L. cv. Mulata) were grown for 9 weeks under different root temperatures (20, 30 and 40°C) at pH values of 5, 7 and 9 with nitrate or ammonium as nitrogen source. Nitrogen uptake rates were determined by depletion from the medium and decreased with distance from the apex. The decline of nitrogen uptake rates along the roots depended on the form of inorganic nitrogen in the medium as well as on pH and temperature, such that the NO− 3 and NH+ 4 ions were taken up essentially by the root tips (0–2 cm) through processes requiring energy. The uncharged NH3 species entered passively, through the mature parts of the root (2–10 cm). Root zone temperature and pH affect the NH+ 4 /NH3 equilibrium in the nutrient solution and, consequently, the uptake areas of the root for these ions. Furthermore. while root tip uptake of nitrogen is energy dependent, uptake through mature root areas is essentially passive and seems to depend on a well developed apparent free space. 相似文献
14.
Abstract The utilization of NO− 3 , NO− 2 and NH+ 4 was studied in whole filaments and isolated heterocysts of Anabaena 7120 (ATCC27893). NO− 3 - and NO− 2 -uptake were detectable in whole filaments but not in heterocysts, whereas NH+ 4 -uptake was detectable in both. Activity of NO− 3 -reductase was present in cell-free extracts of whole filaments but not of heterocysts, whereas activities of NO− 2 -reductase and glutamine synthetase were present in both. NO− 3 -uptake and reductase activities could not be induced in heterocysts even after prolonged incubation in NO− 3 medium. It is suggested that NO− 3 -metabolism in heterocysts is impaired due to a selective and irreversible loss of NO− 3 -uptake and reductase systems resulting in the abolition of competition for molybdenum cofactor (Mo-Co) and reductant between nitrogenase and NO− 3 -reductase, and an increase in glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase levels. 相似文献
15.
Jesús A. G. Ochoa de Alda María I. Tapia Fabrice Franck María J. Llama Juan L. Serra 《Physiologia plantarum》1996,97(1):69-78
In an attempt to clarify the interactions between the available nitrogen source and the photosystems in cyanobacteria, O2 exchange and fluorescence emission were monitored in spheroplasts and intact cells of the non N2 -fixing cyanobacterium Phormidium laminosum (strain OH-1-p.Cl1 ) growing on different nitrogen sources or in the absence of nitrogen. Short-term (time scale of seconds to minutes), NH+ 4 addition to NO− 3 -growing or N-starved cells and, to a minor extent, NO− 3 addition to N-starved cells, induced state 2 transitions both in light and dark. Long term (time scale of days), the fluorescence yield of PSII relative to that of PSII at 77 K was higher in NO− 3 - than in NH+ 4 growing cells, and even higher in N-starved cells. In the dark, the plastoquinone pool was more reduced in NH+ 4 - than in NO− 3 -growing cells. Both PSII and PSI activities and the degree of linking between both photosystems were affected in the long term, so that non-cyclic electron transport decreased in parallel to the ferredoxin requirement to assimilate each nitrogen source. Results indicate that nitrogen metabolism exerts short- and long-term control over the photosynthetic apparatus, which acclimates to the energy requirement of the available nitrogen source. 相似文献
16.
Inhibition of the tempe mould, Rhizopus oligosporus, by ammonia 总被引:1,自引:0,他引:1
The hyphal extension rate of Rhizopus oligosporus NRRL 2710 was slowed in the presence of 0·42 and 0·84 mmol NH3 l−1 and inhibited by 1·3 mmol l−1 . Sporulation was prevented at NH3 concentrations of 0·42 mmol l−1 andabove. There was no evidence of toxicity due to NH+ 4 at concentrations up to 300 mmol l−1 .Independent of the concentrations of NH3 or NH+ 4 , the lower the pH value, in therange 6·0–9·0, the higher was the rate of hyphal extension. It is suggested that accumulationof toxic levels of NH3 could be responsible for the cessation of mould growth in tempe. 相似文献
17.
Effects of nitrogen source on betacyanin accumulation and growth in suspension cultures of Phytolacca americana 总被引:1,自引:0,他引:1
In suspension cultures of Phytolacca americana L., betacyanin accumulation per cell increased with increasing total nitrogen concentration (initial NH+ 4 :NO− 3 ratio 1:2) in the range 0–40 m M and then remained almost constant in the range 40–80 m M . Increasing ammonium increased growth while betacyanin accumulation was reduced. On the other hand, betacyanin accumulation increased when nitrate was increased while growth was almost constant in the concentration range examined. A time-course study of ammonium and nitrate concentration changes in the medium showed that betacyanin accumulation was associated with nitrate uptake. 相似文献
18.
Assimilation of N by heterotrophic soil microbial biomass is associated with decomposition of organic matter in the soil. The form of N assimilated can be either low molecular weight organic N released from the breakdown of organic matter (direct assimilation), or NH+ 4 and NO− 3 from the soil inorganic N pool, into which mineralized organic N is released (mineralization immobilization turnover). The kinetics of C and N turnover in soil is quantifiable by means of computer simulation models. NCSOIL was constructed to represent the two assimilation schemes. The rate of N assimilation depends on the rate of C assimilation and microbial C/N ratio, thereby rendering it independent of the assimilation scheme. However, if any of the N forms is labeled, a different amount of labeled N assimilation will be simulated by the different schemes. Experimental data on inorganic N and 15 N and on organic 15 N dynamics in soils incubated with 15 N added as NH+ 4 or organic N were compared with data simulated by different model schemes. Direct assimilation could not account for the amount of 15 N assimilated in any of the experimental treatments. The best fit of the model to experimental data was obtained for the mineralization immobilization turnover scheme when both NH+ 4 and NO− 3 were assimilated, in proportion to their concentration in the soil. 相似文献
19.
Abstract A comparative study has been undertaken to determine the efficiency of methods for the enrichment and isolation of autotrophic nitrifying bacteria from soils and estuarine and marine sediments. Chemostat enrichments proved to be the most efficient means of isolating autotrophic NH+ 4 oxidisers whereas NO− 2 oxidising bacteria were never successfully enriched by this method. In contrast, gel enrichment and traditional batch culture enrichments of nitrifying bacteria were comparatively time consuming procedures and the degree of enrichment obtained for NH+ 4 oxidising bacteria never approached that obtained with continuous culture enrichments. Gel enrichments, however, because they have continuous physicochemical gradients provide qualitative advantages in that morphologically distinct types of nitrifying bacteria can be isolated from the same gel. 相似文献
20.
In order to investigate effects of limited NO3 availability in corn ( Zea mays L. cv. Brulouis) 17-day-old plants were grown for a further 25 days on sand in a growth chamber. The plants received frequent irrigation with a complete nutrient solution containing 0.2, 0.6, 1.5 or 3.0 mM NO3 . With 0.2 mM NO; nitrate levels in both roots and leaves diminished rapidly and were almost zero after 10 days treatment. Concurrently, as signs of nitrogen deficiency appeared, shoot growth was restricted, whereas root growth was enhanced. In addition, the concentration of reduced nitrogen and malate in the leaves declined, and in vitro nitrate reductase activity (NRA. EC 1.6.6.1), soluble protein and chlorophyll levels of leaf tissue were depressed and starch concentration was enhanced. With 0.6 mM NO3 in the nutrient solution, the decrease in NO3 levels in the tissues and the increase in root development were similar to those observed with 0.2 mM NO3 . However, shoot growth, reduced nitrogen concentration in leaves, and the above-mentioned biochemical characteristics were almost identical to those obtained at 1.5 and 3.0 mM NO3 . This indicates that when supplied with 0.6 mM NO3 , corn plants were able to absorb sufficient NO3 to support maximal biomass production without appreciable NO3 accumulation in roots or shoot. It is, thus, suggested that the plants responded to low NO3 , availability in medium by enhancing root growth and by maximizing NO3 reduction relative to NO3 accumulation. 相似文献