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1.
Shek WR 《ILAR journal / National Research Council, Institute of Laboratory Animal Resources》2008,49(3):316-325
Specific pathogen-free (SPF) rodents for modern biomedical research need to be free of pathogens and other infectious agents that may not produce disease but nevertheless cause research interference. To meet this need, rodents have been rederived to eliminate adventitious agents and then housed in room- to cage-level barrier systems to exclude microbial contaminants. Because barriers can and do fail, routine health monitoring (HM) is necessary to verify the SPF status of colonies. Testing without strict adherence to biosecurity practices, however, can lead to the inadvertent transfer of unrecognized, inapparent agents among institutions and colonies. Microisolation caging systems have become popular for housing SPF rodents because they are versatile and provide a highly effective cage-level barrier to the entry and spread of adventitious agents. But when a microisolation-caged colony is contaminated, the cage-level barrier impedes the spread of infection and so the prevalence of infection is often low, which increases the chance of missing a contamination and complicates the corroboration of unexpected positive findings. The expanding production of genetically engineered mutant (GEM) rodent strains at research institutions, where biosecurity practices vary and the risk of microbial contamination can be high, underscores the importance of accurate HM results in mitigating the risk of the introduction and spread of microbial contaminants with the exchange of mutant rodent strains among investigators and institutions. 相似文献
2.
The mutualistic interaction between scatter-hoarding rodents and their seed plants is highly complex yet poorly understood. Plants may benefit from the seed dispersal behavior of rodents, as long as seed consumption is minimized. In parallel, rodents may maximize foraging efficiency and cache high-quality resources for future consumption. Defensive compounds, such as tannins, are thought to be a major mechanism for plant control over rodent behavior. However, previous studies, using naturally occurring seeds, have not provided conclusive evidence supporting this hypothesis. Here, we test the importance of tannin concentrations on the scatter-hoarding behavior of rodents by using an artificial seed system. We combined feeding trials and field observations to examine the overall impact of seed tannin concentrations on rodent behavior and health. We found that rodents favored seeds with an intermediate amount of tannin (~5%) in the field. Meanwhile, in rodents that were fed a diet with different tannin content, only diets with high tannin content (25%, 15%, and 10%) caused a significant negative influence on rodent survival and health. Significant differences were not found among treatments with tannin levels of 0-5%. In contrast to many existing studies, our results clearly demonstrate that scatter-hoarding rodents prefer slightly 'astringent' food. In the co-evolutionary arms race between plants and animals, our results suggest that while tannins may play a significant role in reducing general predation levels by the faunal community, they have no precise control over the behavior of their mutualistic partner. Instead, the two partners appear to have reached an evolutionary point where both parties receive adequate benefits, with the year-to-year outcome being dependent on a wide range of factors beyond the control of either partner. 相似文献
3.
Besselsen DG Franklin CL Livingston RS Riley LK 《ILAR journal / National Research Council, Institute of Laboratory Animal Resources》2008,49(3):277-290
Rodent parvoviruses, Helicobacter spp., murine norovirus, and several other previously unknown infectious agents have emerged in laboratory rodents relatively recently. These agents have been discovered serendipitously or through active investigation of atypical serology results, cell culture contamination, unexpected histopathology, or previously unrecognized clinical disease syndromes. The potential research impact of these agents is not fully known. Infected rodents have demonstrated immunomodulation, tumor suppression, clinical disease (particularly in immunodeficient rodents), and histopathology. Perturbations of organismal and cellular physiology also likely occur. These agents posed unique challenges to laboratory animal resource programs once discovered; it was necessary to develop specific diagnostic assays and an understanding of their epidemiology and transmission routes before attempting eradication, and then evaluate eradication methods for efficacy. Even then management approaches varied significantly, from apathy to total exclusion, and such inconsistency has hindered the sharing and transfer of rodents among institutions, particularly for genetically modified rodent models that may not be readily available. As additional infectious agents are discovered in laboratory rodents in coming years, much of what researchers have learned from experiences with the recently identified pathogens will be applicable. This article provides an overview of the discovery, detection, and research impact of infectious agents recently identified in laboratory rodents. We also discuss emerging syndromes for which there is a suspected infectious etiology, and the unique challenges of managing newly emerging infectious agents. 相似文献
4.
Functional extinction of a desert rodent: implications for seed fate and vegetation dynamics
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Population declines of once‐abundant species have often preceded understanding of their roles within ecosystems. Consequently, important drivers of environmental change may remain undiagnosed because we simply do not know how species that are now rare or extinct shaped ecosystems in the past. Australia's desert rodents are thought to have little numerical impact on seed fate and vegetation recruitment when compared with ants or with desert rodents on other continents. However most research on granivory by Australian desert rodents has occurred in areas where rodents were rare or functionally extinct. Here we ask if the paradigm that rodents are relatively un‐important granivores in Australian deserts is an artefact of their historical decline. In the Strzelecki Desert, the endangered rodent, Notomys fuscus is rare where introduced mesopredators are abundant but common where dingoes (an apex predator) suppress mesopredator populations. We used foraging trays to compare rates of seed removal for a common shrub (Dodonaea viscosa angustissima hopbush) between areas where N. fuscus, hopbush shrubs and their seedlings were rare and common and found that seed removal was consistently higher where rodents were common and hopbush rare. By excluding ants and rodents from foraging trays we show that ants removed more seeds than rodents where rodents were rare but rodents removed far more seeds than ants where rodents were common. By manipulating rodents’ access to the soil seed‐bank we show that hopbush seeds persisted in greater numbers where rodents were excluded than where they had access. Our results support the hypothesis that granivory by rodents may once have been a far more important process influencing the fate of seeds and shaping plant communities in arid Australia and suggest that dingo extirpation has cascading effects on shrub seeds. Our study highlights that functional extinction of rodents may be an under‐appreciated driver of vegetation change. 相似文献
5.
M. Schwarz A. Buchmann S. Stinchcombe G. Luebeck S. Moolgavkar K. W. Bock 《Mutation research》1995,333(1-2):69-79
The liver is a major target organ in rodent carcinogenicity assays. Amongst the agents that are effective in producing rodent liver tumours are many chemicals which are not mutagenic, but are believed to mediate their effects by promoting the clonal outgrowth of initiated cells. Some of these chemicals, such as dibenzo-p-dioxins and certain PCBs, have been demonstrated to interact with specific cellular receptors and receptor binding appears crucial for their tumourigenic activity. Enzyme-altered foci in rat liver may serve as a sensitive means to estimate the promoting activity of these agents in rodents. Mechanistic considerations are of relevance when extrapolating these date from rodents to humans. 相似文献
6.
Michael J. Lawes Diana O. Fisher Chris N. Johnson Simon P. Blomberg Anke S. K. Frank Susanne A. Fritz Hamish McCallum Jeremy VanDerWal Brett N. Abbott Sarah Legge Mike Letnic Colette R. Thomas Nikki Thurgate Alaric Fisher Iain J. Gordon Alex Kutt 《PloS one》2015,10(6)
Australia has experienced dramatic declines and extinctions of its native rodent species over the last 200 years, particularly in southern Australia. In the tropical savanna of northern Australia significant declines have occurred only in recent decades. The later onset of these declines suggests that the causes may differ from earlier declines in the south. We examine potential regional effects (northern versus southern Australia) on biological and ecological correlates of range decline in Australian rodents. We demonstrate that rodent declines have been greater in the south than in the tropical north, are strongly influenced by phylogeny, and are consistently greater for species inhabiting relatively open or sparsely vegetated habitat. Unlike in marsupials, where some species have much larger body size than rodents, body mass was not an important predictor of decline in rodents. All Australian rodent species are within the prey-size range of cats (throughout the continent) and red foxes (in the south). Contrary to the hypothesis that mammal declines are related directly to ecosystem productivity (annual rainfall), our results are consistent with the hypothesis that disturbances such as fire and grazing, which occur in non-rainforest habitats and remove cover used by rodents for shelter, nesting and foraging, increase predation risk. We agree with calls to introduce conservation management that limits the size and intensity of fires, increases fire patchiness and reduces grazing impacts at ecological scales appropriate for rodents. Controlling feral predators, even creating predator-free reserves in relatively sparsely-vegetated habitats, is urgently required to ensure the survival of rodent species, particularly in northern Australia where declines are not yet as severe as those in the south. 相似文献
7.
Nicklas W 《ILAR journal / National Research Council, Institute of Laboratory Animal Resources》2008,49(3):338-346
Proper health surveillance is vitally important to the evaluation of the microbial status of laboratory animals and the performance of standardized experiments with a minimum number of animals. Sufficient and reliable information about animals health status has become even more important during the last decade with the rapid development and worldwide exchange of new genetically modified rodents. But a universal testing strategy for the assessment of pathogen status in rodent populations and internationally recognized standards and definitions of their quality do not exist, even though health data can provide consistent information only when based on systematic sampling and testing. Although there have been repeated calls for the development of international health monitoring standards and reporting, there are also objections. This article presents both the advantages and limitations of guidelines. After an overview of major factors to consider I discuss previous attempts to harmonize health monitoring procedures. The health monitoring recommendations for rodents issued by the Federation of European Laboratory Science Associations (FELASA) could serve as a model for global recommendations and for international harmonization. Given the increased significance of accurate health information when exchanging animals, research institutions and universities would benefit from universal standards, which would also help scientists as well as reviewers and readers of publications to better assess the validity of research results. 相似文献
8.
Madougou Garba Ambroise Dalecky Ibrahima Kadaoure Mamadou Kane Karmadine Hima Sophie Veran Sama Gagare Philippe Gauthier Caroline Tatard Jean-Pierre Rossi Gauthier Dobigny 《PloS one》2014,9(11)
Invasive rodents have been responsible for the diffusion worldwide of many zoonotic agents, thus representing major threats for public health. Cities are important hubs for people and goods exchange and are thus expected to play a pivotal role in invasive commensal rodent dissemination. Yet, data about urban rodents'' ecology, especially invasive vs. native species interactions, are dramatically scarce. Here, we provide results of an extensive survey of urban rodents conducted in Niamey, Niger, depicting the early stages of rodent bioinvasions within a city. We explore the species-specific spatial distributions throughout the city using contrasted approaches, namely field sampling, co-occurrence analysis, occupancy modelling and indicator geostatistics. We show that (i) two species (i.e. rural-like vs. truly commensal) assemblages can be identified, and that (ii) within commensal rodents, invasive (Rattus rattus and Mus musculus) and native (Mastomys natalensis) species are spatially segregated. Moreover, several pieces of arguments tend to suggest that these exclusive distributions reflect an ongoing native-to-invasive species turn over. The underlying processes as well as the possible consequences for humans are discussed. 相似文献
9.
虽然实验鼠种群的总体健康水平得到了显著的提高,但还是有很多重要的传染性病原体在实验鼠群中流行.实验鼠群的健康状况对于动物福利、科学研究甚或人类健康都很重要.所以必须要对实验鼠群进行健康监测.笔者依据自身的实践和经验,对鼠群健康监测的基本原理进行了分析,包括以下的内容:鼠群健康监测的必要性、健康检测规程的建立、哨兵鼠、常用的监测力法、样本采集原则、对检测结果的解读和应对.相信读者在了解这些基本的原理后,能设定出符合自身设施实际的鼠群健康监测计划并逐步在实践中完善. 相似文献
10.
Parasite species loads are expected to be higher in the tropics and higher parasite species richness to have cumulative effects on host physiology or demography. Despite being regularly assumed or predicted, empirical evidence on species–latitude patterns is scarce or contradictory and studies on the impacts of concomitant infections have mainly been done at host intra‐specific level. Broad generalizations are then very hard, if not spurious. By focusing on rodent species and their non‐eukaryotic microparasites (i.e. viruses and bacteria), we investigated, using a comparative approach, microparasite species richness across rodent species according to the latitude where they occur. We also explored the links between rodents’ reproductive traits, latitude and microparasite species richness. We find for the first time in rodents that virus species richness increases towards tropical latitudes, and that rodent litter size seems to decrease when microparasite species richness increases independently from the latitude. These results support the hypotheses that rodent species in the tropics effectively harbour higher parasite species loads, at least in terms of species richness for viruses, and that parasite species richness influences rodent life‐history traits. Although some other factors, such as seasonality, were not taken into account due the lack of data, our study stresses the idea that chronic microparasite infections may have detrimental effects on their rodent host reservoirs, notably by affecting litter size. 相似文献
11.
While niche separation and relationships with environmental conditions of large mammals of the African savanna have been studied intensively, less conspicuous components have not received similar attention. This is the case of Kafue National Park (KNP), Zambia, where mechanisms supporting coexistence among rodent and shrew species remain unclear, much less the influence of fire on their dietary resource use. Here, we use stable carbon and nitrogen isotopes to assess dietary resource use and partitioning among rodents and shrews found in three vegetation formations of KNP. According to the nitrogen isotope signatures, rodents are one to two trophic levels above primary production, save for Mus triton that is above by two to three. Shrews are two trophic levels above primary production. Among shrews, factors allowing coexistence of similar sized species could not be resolved. Rodent species of the same assemblage either differ in body mass by a factor of two (following Hutchinson's rule) or similar sized species occupy different trophic levels or dietary guilds based on their isotopic nitrogen or carbon signatures. At sites with frequent fires, rodents have broader dietary niches than at sites with low fire frequencies. This could either indicate relaxed competition under high fire frequencies as rodent populations do not reach the carrying capacity of the habitat, or it could reflect reduced competition due to lower species numbers under high versus low fire recurrence regimes. The results indicate competition as an important component structuring rodent communities in Zambian savannas, thus suggesting limited resources. 相似文献
12.
实验用生物样品在生物医学研究中有着广泛的应用.生物样品的微生物污染可危及实验动物设施的生物安全以及对动物实验研究造成负面影响.因此在将生物样品接种到啮齿类动物之前,要对其进行检测以确保无啮齿类动物传染性病原体的污染.因为检测技术和经济上的限制和局限性,不可能对所有的啮齿类动物传染性病原都进行检测.本文依据科学文献、笔者的经验以及专家的意见,尝试着去阐述如何建立一个有效可行的生物样品微生物污染检测计划.笔者建议对一些重要的微生物进行检测,并简要综述了选取这些微生物的理由即它们对科研、实验动物生物安全以及职业健康安全的影响. 相似文献
13.
啮齿动物群落结构可以反映生态环境特征。本研究对处于阿拉善荒漠区呈“孤岛”状态的内蒙古贺兰山国家级自然保护区内啮齿动物的种类、分布型及群落多样性进行系统研究。将内蒙古贺兰山划分为五种生境类型,于2013年春、夏、秋3季共布设有效铗日18748个,捕获啮齿动物235只,分属2目5科11属13种。在整体研究区域,阿拉善黄鼠(Spermophilus alashanicus)为优势种。明确了喜湿型是本研究区域的主要分布型,占捕获啮齿动物的54%,并且在中低海拔区域,随着海拔的升高,喜湿型所占比例增加。受“边缘效应”的影响,山地荒漠和荒漠草原生境以及山地草原灌丛生境的啮齿动物群落多样性指数高于其他生境,除环境因素外,啮齿动物群落的多样性受两种因素的影响,既可随群落内物种数量的增加而增大,同时又受制于群落内部物种分布的均匀程度。冗余分析结果表明,植被高度、植被盖度、灌木(乔木)高度和海拔4个环境因子是决定啮齿动物群落结构最主要的环境因子,其中植被盖度与群落多样性呈负相关,随着植被盖度的增加,多样性指数随之减低。 相似文献
14.
An analysis of 54 protein sequences from humans and rodents (mice or rats), with the chicken as an outgroup, indicates that, from the common ancestor of primates and rodents, 35 of the proteins have evolved faster in the lineage to mouse or rat (rodent lineage) whereas only 12 proteins have evolved faster in the lineage to humans (human lineage). The average rate of amino acid substitution is significantly faster in the rodent lineage than in the human lineage. In addition, the average rate of insertion/deletion is also faster in rodents than in humans and there is a positive correlation between the rate of amino acid substitution and the rate of insertion/deletion in a protein sequence. 相似文献
15.
Partial plastid 23S and nuclear 18S rDNA genes were amplified and sequenced from 2 morphologically similar Eimeria species. E. antrozoi from a bat (Antrozous pallidus) and E. arizonensis from deer mice (Peromyscus spp.), as well as some other Eimeria species from bats and rodents. The phylogenetic trees clearly separated E. antrozoi from E. arizonensis. The phylogenies based on plastid 23S rDNA data and combined data of both plastid and nuclear genes grouped 2 bat Eimeria and 3 morphologically similar Eimeria species from rodents into 2 separate clades with high bootstrap support (100%, 3 rodent Eimeria species; 72-97%, 2 bat Eimeria species), which supports E. antrozoi as a valid species. The rodent Eimeria species did not form a monophyletic group. The 2 bat Eimeria species formed a clade with the 3 morphologically similar rodent Eimeria species (E. arizonensis, E. albigulae, E. onychomysis, all from cricetid rodents) with 100% bootstrap support, whereas 2 other rodent Eimeria species (E. nieschulzi, E. falciformis, from murid rodents) formed a separate clade with 100% bootstrap support. This suggests that the 2 Eimeria species from bats might be derived from rodent Eimeria species and may have arisen as a result of lateral host transfer between rodent and bat hosts. 相似文献
16.
1. We investigate the effects of different levels of predation pressure and rodent dispersal on the population dynamics of the African pest rodent Mastomys natalensis in maize fields in Tanzania. 2. Three levels of predation risk were used in an experimental set-up: natural level (control), excluding predators by nets and attracting avian predators by nest boxes and perch poles. Because dispersal of the rodents could mask the predation pressure treatment effects, control and predator exclusion treatments were repeated with enclosed rodent populations. 3. Population growth during the annual population rise period was faster in the absence of predators and peak population size was higher, but otherwise dynamics patterns were similar for populations where predators had access or were attracted, indicating that compensatory mechanisms operate when rodents are exposed to high levels of predation risk. Reducing dispersal of rodents removed the effect of predation on population growth and peak size, suggesting that local predators may play a role in driving rodent dispersal, but have otherwise little direct effect on population dynamics. 相似文献
17.
On the basis of 18 protein sequences totaling 2,413 aligned amino acid sites, it is suggested that the guinea pigs and the myomorphs (rat-like rodents) are not monophyletic. Rather, the evolutionary lineage leading to the guinea pig seems to have branched off prior to the divergence among myomorphs, lagomorphs, primates, chiropterans, artiodactyls, and carnivores. It is suggested therefore that the Caviomorpha (guinea pig-like rodents) and possibly the Hystricomorpha (porcupine-like rodents) should be elevated in taxonomic rank and conferred an ordinal status distinct from the Rodentia. This suggestion calls for a reevaluation of the morphological evolution of guinea pigs and further molecular studies on the possibility of paraphyly of the order Rodentia. If the monophyly of rodents holds, it must be concluded that the pattern of molecular evolution in many guinea pig genes has been extremely unusual and that the causes for this pattern should be sought. It is also suggested that claims of large differences in the rate of molecular evolution between guinea pigs and myomorphs may have been exaggerated in many cases as a result of an erroneous phylogenetic position for the guinea pig. The average rate of amino acid replacement in the guinea pig seems to be comparable to that in the rat and the mouse. However, the data indicate that myomorph and caviomorph genes evolve, on average, about two times faster than their human counterparts. Finally, our analysis provides evidence against the hypothesis that the gundi (an African rodent) represents the most ancient rodent lineage. 相似文献
18.
S W Barthold 《The Journal of protozoology》1991,38(6):131S-133S
The influence of viruses and bacteria on Pneumocystis carinii pneumonia (PCP) is suspected but not known for certain. A number of adventitious viruses and bacteria are common among commercially available and institutionally raised rodents, which may impact upon, or interfere with, the induction of PCP in rodents in several ways. Based upon the biological behavior and prevalence of certain rodent agents, the potential for such impact is great. Infectious agents can directly interfere with research by inducing severe disease in the immunocompromised host. They may also influence the course of P. carinii infection or disease via direct effects upon the respiratory tract or via indirect effects on lymphoreticular cells or other organ systems. Examples of likely agents and their potential effects are discussed. 相似文献
19.
开垦对功能相对脆弱的荒漠生态系统是重要的干扰,这种干扰往往导致栖息地破碎化,并对动植物群落产生强烈影响。作为荒漠生态系统的重要成分,啮齿动物群落受到开垦干扰后对环境的响应及其群落中种群的生态对策,是荒漠生态系统生物多样性及其功能维持稳定的重要基础。2006~2011年,采用标志重捕法对内蒙古阿拉善荒漠区未开垦和开垦草地啮齿动物群落格局及其不同生态对策种群的数量组成进行了专门研究。目的(1)明确开垦干扰下啮齿动物群落格局时间尺度变化;(2)验证假设:人为干扰区(开垦区)啮齿动物群落中r?对策者占据优势,未干扰区(未开垦区)以K?对策者为主;(3)依据啮齿动物群落中不同生态对策种群的数量组成来判断群落所受干扰的程度。结果表明,开垦干扰降低了啮齿动物群落多样性,改变了群落中不同生态对策种群的数量组成,种群以r?对策者为优势;未开垦区啮齿动物群落中以K?对策种群为主。开垦区啮齿动物群落受到严重干扰。 相似文献
20.
GS Sun JC Tou DA Reiss-Bubenheim EL Hill KW Liittschwager BE Girten E Pena-Yewkukhiw 《Lab animal》2012,41(9):252-259
The National Aeronautics and Space Administration's standard spaceflight diet for rodents is the nutrient-upgraded rodent food bar (NuRFB). The shelf life of the NuRFB needs to be determined in order to avoid malnutrition of rodents and confounding of research results resulting from nutritional deficiency. The authors compared the oxidative and nutrient stability of NuRFBs stored at either ambient temperature (26 °C) or at refrigeration temperature (4 °C) for use in long-term rodent feeding experiments. After 0, 3, 6, 9 and 12 months (mo) of storage, lipid oxidation, fatty acid composition and amounts of specific vitamins and amino acids in NuRFBs were analyzed. No oxidative rancidity developed in NuRFBs stored at 4 °C for up to 12 mo, but NuRFBs stored at 26 °C for 6 mo developed oxidative rancidity and had reduced amounts of γ-linolenic acid (18:3n-6). Despite loss of vitamin E, vitamin A and thiamin after storage at 26 °C for 12 mo, vitamin levels in NuRFBs remained at or above the levels recommended for optimal rodent health. The amino acid profile of NuRFBs was unaffected by storage at 4 °C or 26 °C for 12 mo. The results suggest that NuRFBs stored at 4 °C for up to 12 mo and NuRFBs stored at 26 °C for up to 6 mo provide suitable nutrition for rodents in long-term experiments. 相似文献