Studies on the interspecific competition between Helisoma duryi (wetherby) and Biomphalaria camerunensis (boettger). Size-weight relationships and laboratory competition experiments

Biological control of the intermediate hosts of schistosomiasis by means of the introduction of a competitor snail Helisoma duryi has been suggested. In the present laboratory studies the allometric size-weight relationships of H. duryi and Biomphalaria camerunensis were investigated in order to get a tool for estimating the biomass and dry-weight of snails.Competition experiments were conducted and the results showed a marked inhibition of growth and reproduction of B. camerunensis. No conclusions about the competitive interactions were drawn from the present results but experiments are established to elucidate these mechanisms.     

Spatial microhabitat selection by Biomphalaria pfeifferi in a small perennial river in Tanzania

A study was carried out in the Mlali river in south-central Tanzania with two aims. First, to determine microhabitat availability in two sites (A and B) with respect to water depth, water velocity and dominant substratum type. Second, to assess microhabitat use by Biomphalaria pfeifferi, the intermediate host snail of intestinal schistosomiasis and to investigate whether these snails show preferences for certain microhabitats. The two sites differed significantly with respect to width, water depth, water velocity and substratum composition. It is suggested that the absence of B. pfeifferi from site B is mainly associated with the high water velocities at that site, where 62% of the measurements exceeded30 cm s^-1. In site A, the microhabitat use by 327 B. pfeifferi snails was assessed by means of direct observation. No significant relationships were found between snail size and the habitat variables investigated, indicating that snail size appeared to be of no importance in spatial microhabitat selection. B. pfeifferi snails showed statistically significant preferences for shallow water (depth: 2–7 cm) and the preferred water velocities ranged between 12 and 21 cm s^-1 with an estimated optimum at13.3 cm s^-1. No statistically significant preferences for substratum type were found. This revised version was published online in August 2006 with corrections to the Cover Date.     

Aquatic snails of the Jonglei region,southern Sudan,and transmission of trematode parasites

An account is given of the aquatic gastropod molluscs obtained in a survey made to assess future ecological effects of the Jonglei Canal now under construction in the Sudd region of southern Sudan. A total of about 23 species of aquatic snail lives in this region, of which a number are utilised as intermediate hosts by trematode parasites infecting man, livestock and wild herbivores, causing the diseases schistosomiasis, fascioliasis and amphistomiasis. Public health and veterinary authorities should be alert to the possibility that the Jonglei Canal will increase snail populations and raise the prevalence of parasitic diseases.Jonglei Range Ecology Survey.     

Microsatellites in the freshwater snail Bulinus globosus (Gastropoda: Planorbidae) from Zanzibar

Six microsatellite loci were isolated and characterized in Bulinus globosus, a freshwater snail with a wide distribution throughout sub‐Saharan Africa. Bulinus globosus is an intermediate host of Schistosoma haematobium, the causative agent of human urinary schistosomiasis. Microsatellites were tested using 32 snails from four populations collected from Pemba and Unguja islands of Zanzibar. The microsatellite loci displayed relatively low levels of variation, with between two and five alleles per locus. F_ST estimates indicate that gene flow is low, as has previously been suggested for other species of Bulinus.     

Food overlap in co-existing populations of the land snails Cepaea nemoralis (L.) and Cepaeahortensis (Müll)

Faecal analysis of adult Cepaea nemoralis and Cepaea hortensis from a mixed population on chalk grassland shows that the two snail species select the same plant material as food. Herbs are selected in preference to grasses and Urtica dioica is particularly favoured. C. hortensis has the more pronounced preference for senescent material. These results are discussed in relation to competition between the two species.     

Polymorphic microsatellites in the African freshwater snail,Bulinus forskalii (Gastropoda,Pulmonata)

Eleven microsatellites were isolated in the freshwater snail Bulinus forskalii, intermediate host for the medically important trematode Schistosoma intercalatum. Characterization in 60 snails from three populations of B. forskalii from Cameroon revealed 4 to 18 alleles per locus. Low observed heterozygosity but higher expected heterozygosity, high F_IS estimates, significant departures from Hardy–Weinberg equilibrium and genotypic linkage disequilibria all indicate that B. forskalii is a preferential selfer. High F_ST estimates suggest that effective dispersal is limited and genetic drift is an important determinant of genetic structure. The potential utility of the microsatellite primers in other closely related Bulinus species was explored.     

Growth and oviposition of the freshwater pulmonate Bulinns tropicus (Gastropoda) reared in the laboratory under reversed, extreme and irregular ultradian light-dark cycles

The effect of irregular photoperiods on various growth parameters and oviposition of a tropical freshwater pulmonate, Bulinus tropicus , has been investigated. Over a period of 6 weeks the growth (measured as an increase in wet weight) of juvenile snails was unaffected by transfer to reversed and to random non-circadian light-dark regimens, although oviposition was considerably retarded in the latter experimental group and its time of onset was delayed relative to the control group held in a 12 h light-12 h dark regimen. Snails exposed to constant light grew more, and their oviposition rate (number of eggs snail^-1 week^-1) was equal to that of the control group, but these two parameters were found to be considerably reduced in snails kept in constant darkness. The food consumption, assimilation and food conversion efficiency of snails in random and reversed light-dark regimens were comparable to the control groups, whereas animals in constant light ate more and those in constant darkness ate less. The weekly mean assimilation rate and food conversion efficiency were greater in the latter group, however.     

Distribution of freshwater snails in the river Niger basin in Mali with special reference to the intermediate hosts of schistosomes

Snail surveys were carried out in various parts of Mali. All areas surveyed are part of the Niger basin being either affluents or irrigation schemes fed by this river. The snail species present varied greatly between areas. The following potential hosts of schistosomes were recorded: Biomphalaria pfeifferi, Bulinus truncatus, B. globosus, B. umbilicatus, B. forskalii and B. senegalensis. In the large irrigation schemes, i.e. ‘Office du Niger’ and Baguinéda, only B. pfeifferi and B. truncatus appear to be intermediate hosts. Snail distribution appeared to some extent to be focal and high snail densities appeared to be associated with human water contact activities, which apparently create favourable biotopes for the snails. This is probably due to an alteration of the vegetation and an increase of the trophic status of the site by contamination with food remnants and other debris. The larger irrigation canals or lakes in these schemes play an important role in the transmission of human schistosomes and transmission appears to be very focal in these habitats. Infected snails are almost exclusively found in well-defined human water contact sites (WCS). Local infection rates with schistosomes were often high (i.e. up to 27% in B. pfeifferi). In urban areas (i.e. Bamako), transmission patterns are more variable. In Bamako schistosome-infected B. truncatus were found in the Niger river. A number of smaller semi-permanent or permanent streams are very important transmission sites, and schistosome infections were recorded from B. pfeifferi, B. truncatus and B. globosus. Schistosome infection rates in B. pfeifferi were often high (up to 30%). In a new lake at Sélingué, B. truncatus was found to be widely distributed only about a year and a half after the dam was constructed, and in some sites schistosome infections were recorded.     

Food and feeding habits of Labeo niloticus (Pisces,Cyprinidae) in Jebel Aulia Reservoir,Sudan

Basic knowledge on the feeding ecology of one of the common and commercially important fish species in Jebel Aulia Reservoir is provided.The structure of the feeding apparatus indicates that Labeo niloticus is a bottom feeder, depending on soft and decayed vegetation, organic debris and whatever small organisms found within. However, juveniles and fry are prone to explore all layers and depths of the river selectively for plankton. There is little evidence of seasonal selection of food. Changes in diet quality are governed by the availability of type of food. Variability of feeding activity is connected with climate and breeding season.     

Food limitation of population density in the orb-wed spider,Nephila clavata

Field studies were conducted to clarify whether variation in food availability among habitats influences population density, and whether population density has a negative effect on foraging success in the orb-web spider, Nephila clavata. Lifetime food consumption per individual (i.e., foraging success) strongly correlated with mean body size of adult females and mean fecundity in populations. Also, there was a positive correlation between foraging success and population density. Since foraging success reflected potential prey availability in the habitat, food resource appeared to be a limiting factor for populations in this spider. Mean fecundity per individual correlated with population density of the following year, suggesting that decreased reproduction is a major component of food limitation on population density. Consistent defferences in mean body size between particular sites were observed over years, while such difference was less obvious in density. Thus, ranking of food abundance among habitats seems to be predictable between years. A field experiment revealed that an artificial increase in population density had no negative effect on the feeding rate of individuals, suggesting that intraspecific competition for food is not important in this species.     

Sexual selection in a lekking bird: the relative opportunity for selection by female choice and male competition

Leks are classic models for studies of sexual selection due to extreme variance in male reproductive success, but the relative influence of intrasexual competition and female mate choice in creating this skew is debatable. In the lekking lance-tailed manakin (Chiroxiphia lanceolata), these selective episodes are temporally separated into intrasexual competition for alpha status and female mate choice among alpha males that rarely interact. Variance in reproductive success between status classes of adult males (alpha versus non-alpha) can therefore be attributed to male-male competition whereas that within status largely reflects female mate choice. This provides an excellent opportunity for quantifying the relative contribution of each of these mechanisms of sexual selection to the overall opportunity for sexual selection on males (I males). To calculate variance in actual reproductive success, we assigned genetic paternity to 92.3% of 447 chicks sampled in seven years. Reproduction by non-alphas was rare and apparently reflected status misclassifications or opportunistic copulations en route to attaining alpha status rather than alternative mating strategies. On average 31% (range 7-44%, n=6 years) of the total I males was due to variance in reproductive success between alphas and non-alphas. Similarly, in a cohort of same-aged males followed for six years, 44-58% of the total I males was attributed to variance between males of different status. Thus, both intrasexual competition for status and female mate choice among lekking alpha males contribute substantially to the potential for sexual selection in this species.     

Competitive PCR reveals the complexity of postcopulatory sexual selection in Teleogryllus commodus

The outcome of mate choice depends on complex interactions between males and females both before and after copulation. Although the competition between males for access to mates and premating choice by females are relatively well understood, the nature of interactions between cryptic female choice and male sperm competition within the female reproductive tract is less clear. Understanding the complexity of postcopulatory sexual selection requires an understanding of how anatomy, physiology and behaviour mediate sperm transfer and storage within multiply mated females. Here we use a newly developed molecular technique to directly quantify mixed sperm stores in multiple mating females of the black field cricket, Teleogryllus commodus. In this species, female postcopulatory choice is easily observed and manipulated as females delay the removal of the spermatophore in favour of preferred males. Using twice‐mated females, we find that the proportion of sperm in the spermatheca attributed to the second male to mate with a female (S₂) increases linearly with the time of spermatophore attachment. Moreover, we show that the insemination success of a male increases with its attractiveness and decreases with the size of the female. The effect of male attractiveness in this context suggests a previously unknown episode of mate choice in this species that reinforces the sexual selection imposed by premating choice and conflicts with the outcome of postmating male harassment. Our results provide some of the clearest evidence yet for how sperm transfer and displacement in multiply mated females can lead directly to cryptic female choice, and that three distinct periods of sexual selection operate in black field crickets.     

Low genetic diversity in a snail intermediate host (Biomphalaria pfeifferi Krass, 1848) and schistosomiasis transmission in the Senegal River Basin

Population genetic perturbations of intermediate hosts, often a consequence of human pressure on environmental resources, can precipitate unexpectedly severe disease outbreaks. Such disturbances are set to become increasingly common following range changes concomitant with climate shifts, dwindling natural resources and major infrastructure changes such as hydroprojects. Construction of the Diama dam in the Senegal River Basin (SRB) reduced river salinity, enabling the freshwater snail intermediate host Biomphalaria pfeifferi to rapidly expand its distribution. A serious public health problem ensued, with an epidemic of intestinal schistosomiasis occurring in the previously schistosome‐free Richard‐Toll region within 2 years. The current study aimed to assess the population variability of B. pfeifferi in the SRB, and speculate upon its subsequent impact on host‐parasite interactions following such engineered ecological change. Genetic variation at nine polymorphic microsatellite loci revealed little population differentiation in SRB snails compared with those from natural habitats in Zimbabwe, where Schistosoma mansoni transmission is much lower. ‘Open’ SRB habitats are associated with greater water contact, smaller population sizes and less genetic diversity, with sites downstream of Richard‐Toll showing greater inter‐ and intrapopulation variation, concomitant with less frequent human contact. These observations may be explained by rapid expansion into pristine habitat selecting for high fecundity genotypes at the expense of schistosome resistance, presenting S. mansoni with genetically homogenous highly fecund susceptible populations around the focal point, promoting development of a highly compatible host‐parasite relationship. Longitudinal study of such systems may prove important in predicting public health risks engendered by future environmental engineering projects.     

Repertoire size, sexual selection, and offspring viability in the great reed warbler: changing patterns in space and time

Only a few studies have focussed on the consistency of sexualselection patterns in space and time. One such case is the greatreed warbler (Acrocephalus arundinaceus), for which studiesin Germany in 1981–1982 and Sweden in 1987–1991strongly suggested that the size of a male's song repertoirewas the target of mate choice and sexual selection. Studyingthe same German population once again in 1994–2000, weinvestigated the consistency of these patterns over time aswell as between populations. Our reanalysis of the data from1981–1982 shows that male repertoire size was positivelycorrelated with male pairing success (harem size) and with clutchsize (adjusted for seasonal effects), whereas no such correlationswere found during 1994–2000 in the same population. Wesuggest that the earlier correlations were probably caused indirectlyby covariation with territory quality, and that a decline inpopulation size has changed the role of territory quality. Inthe Swedish population, an earlier study found a striking correlationbetween the size of a male's repertoire and the viability ofits offspring, suggesting good-gene effects. In contrast, wefound no such correlation in the German population, neitherin 1981–1982 nor in 1994–2000. We conclude thatrepertoire size does not seem to be a very reliable indicatorof variation in male quality. Interestingly, the analysis ofdata from 1994–2000 showed that male pairing success wasstrongly correlated with measures of strophe length and immediateversatility, traits that have been found to reflect male longevity.Future studies will have to show whether these performance-relatedtraits are more powerful indicators of male quality than isrepertoire size.     

Food selection by brook trout in a subalpine stream

The diet and food selection of brook trout (Salvelinus fontinalis) and macroinvertebrate drift are described for a high altitude stream (3 205 m above mean sea level) adjacent to the alpine zone on the Snowy Range in the Medicine Bow National Forest, Wyoming. Diet composition differed between brook trout of two length classes, 150 mm and > 150 mm in total length. The number of prey organisms per stomach generally declined and the proportion of terrestrial prey items increased from July to September, 1985. Fish of both length classes selected for Trichoptera and Coleoptera over the three sampling months, and fish > 150 mm also selected for terrestrial insects in August and September.The Unit is jointly supported by the University of Wyoming, the Wyoming Game and Fish Department, and the US Fish and Wildlife ServiceThe Unit is jointly supported by the University of Wyoming, the Wyoming Game and Fish Department, and the US Fish and Wildlife Service     

贵州麻阳河黑叶猴秋季主要食物营养分析及对食物选择的影响

2009年9～11月,采集贵州麻阳河黑叶猴取食较多的23种主要食物及取食较少的14种非主要食物,用称重法、凯氏定氮法、酸碱洗涤法及范式洗涤法分析各独立样本的水分、粗蛋白、粗纤维和酸性洗涤纤维(ADF)的营养含量,计算相应的粗蛋白/酸性洗涤纤维(PROT/ADF)比值,并将所得到的各样本的营养含量与相应的秋季取食比例进行...     

Breeding system and demography shape population genetic structure across ecological and climatic zones in the African freshwater snail, Bulinus forskalii (Gastropoda, Pulmonata), intermediate host for schistosomes

The role of breeding system and population bottlenecks in shaping the distribution of neutral genetic variation among populations inhabiting patchily distributed, ephemeral water bodies was examined for the hermaphroditic freshwater snail Bulinus forskalii, intermediate host for the medically important trematode Schistosoma guineensis. Levels of genetic variation at 11 microsatellite loci were assessed for 600 individuals sampled from 19 populations that span three ecological and climatic zones (ecozones) in Cameroon, West Africa. Significant heterozygote deficiencies and linkage disequilibria indicated very high selfing rates in these populations. Despite this and the large genetic differentiation detected between populations, high levels of genetic variation were harboured within these populations. The high level of gene flow inferred from assignment tests may be responsible for this pattern. Indeed, metapopulation dynamics, including high levels of gene flow as well as extinction/contraction and recolonization events, are invoked to account for the observed population structuring, which was not a consequence of isolation-by-distance. Because B. forskalii populations inhabiting the northern, Sahelian area are subject to more pronounced annual cycles of drought and flood than the southern equatorial ones, they were expected to be subject to population bottlenecks of increased frequency and severity and, therefore, show reduced genetic variability and elevated population differentiation. Contrary to predictions, the populations inhabiting the most northerly ecozone exhibited higher genetic diversity and lower genetic differentiation than those in the most southerly one, suggesting that elevated gene flow in this region is counteracting genetic drift.