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1.
Julia T. Apter 《Bulletin of mathematical biology》1965,27(1):27-52
The aortic pressure curve necessarily reveals the mechanical properties of the aorta and peripheral resistance as well as of the dynamics of blood flow. The present study uses a reasonable model of visco-elastic properties of the aorta, a reasonable form for variations in peripheral resistance and blood flow to predict an aortic pressure tracing. Numerical values of constants measured experimentally were available in the published literature. These were used in the nonlinear differential equations of motion of the system under analysis. The equations yielded to piece-wise solution, giving the aortic circumference and the aortic pressure as functions of time. The form of both curves resembles clinical tracings, but numerical values of circumference were higher and of pressure lower thanin vivo. The discrepancies between predicted and clinical curves may reveal certain inadequacies in published measurements on visco-elastic constants. These measurements have been made on longitudinal rather than circumferential strips often containing dead rather than living muscle. The discrepancies, therefore, indicate specific gaps in our knowledge of aortic behaviorin vitro. The suggested model of the system aided in the design of experiments which could supply data necessary to substantiate or to revise the model. 相似文献
2.
Christopher J. Nicholson Francesca Seta Sophie Lee Kathleen G. Morgan 《Journal of cellular and molecular medicine》2017,21(1):81-95
Increased aortic stiffness is a biomarker for subsequent adverse cardiovascular events. We have previously reported that vascular smooth muscle Src‐dependent cytoskeletal remodelling, which contributes to aortic plasticity, is impaired with ageing. Here, we use a multi‐scale approach to determine the molecular mechanisms behind defective Src‐dependent signalling in an aged C57BL/6 male mouse model. Increased aortic stiffness, as measured in vivo by pulse wave velocity, was found to have a comparable time course to that in humans. Bioinformatic analyses predicted several miRs to regulate Src‐dependent cytoskeletal remodelling. qRT‐PCR was used to determine the relative levels of predicted miRs in aortas and, notably, the expression of miR‐203 increased almost twofold in aged aorta. Increased miR‐203 expression was associated with a decrease in both mRNA and protein expression of Src, caveolin‐1 and paxillin in aged aorta. Probing with phospho‐specific antibodies confirmed that overexpression of miR‐203 significantly attenuated Src and extracellular signal regulated kinase (ERK) signalling, which we have previously found to regulate vascular smooth muscle stiffness. In addition, transfection of miR‐203 into aortic tissue from young mice increased phenylephrine‐induced aortic stiffness ex vivo, mimicking the aged phenotype. Upstream of miR‐203, we found that DNA methyltransferases (DNMT) 1, 3a, and 3b are also significantly decreased in the aged mouse aorta and that DNMT inhibition significantly increases miR‐203 expression. Thus, the age‐induced increase in miR‐203 may be caused by epigenetic promoter hypomethylation in the aorta. These findings indicate that miR‐203 promotes a re‐programming of Src/ERK signalling pathways in vascular smooth muscle, impairing the regulation of stiffness in aged aorta. 相似文献
3.
Vascular smooth muscle cells direct extracellular dysregulation in aortic stiffening of hypertensive rats
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Aortic stiffening is an independent risk factor that underlies cardiovascular morbidity in the elderly. We have previously shown that intrinsic mechanical properties of vascular smooth muscle cells (VSMCs) play a key role in aortic stiffening in both aging and hypertension. Here, we test the hypothesis that VSMCs also contribute to aortic stiffening through their extracellular effects. Aortic stiffening was confirmed in spontaneously hypertensive rats (SHRs) vs. Wistar‐Kyoto (WKY) rats in vivo by echocardiography and ex vivo by isometric force measurements in isolated de‐endothelized aortic vessel segments. Vascular smooth muscle cells were isolated from thoracic aorta and embedded in a collagen I matrix in an in vitro 3D model to form reconstituted vessels. Reconstituted vessel segments made with SHR VSMCs were significantly stiffer than vessels made with WKY VSMCs. SHR VSMCs in the reconstituted vessels exhibited different morphologies and diminished adaptability to stretch compared to WKY VSMCs, implying dual effects on both static and dynamic stiffness. SHR VSMCs increased the synthesis of collagen and induced collagen fibril disorganization in reconstituted vessels. Mechanistically, compared to WKY VSMCs, SHR VSMCs exhibited an increase in the levels of active integrin β1‐ and bone morphogenetic protein 1 (BMP1)‐mediated proteolytic cleavage of lysyl oxidase (LOX). These VSMC‐induced alterations in the SHR were attenuated by an inhibitor of serum response factor (SRF)/myocardin. Therefore, SHR VSMCs exhibit extracellular dysregulation through modulating integrin β1 and BMP1/LOX via SRF/myocardin signaling in aortic stiffening. 相似文献
4.
The passive anisotropic elastic properties of rat's aorta were studied in vitro by subjecting cylindrical segments of thoracic and abdominal aorta to a wide range of deformations. Using data on pressure, axial stretch, outer diameter, axial force and wall thickness, incremental moduli of elasticity in the circumferential, axial and radial directions were computed. Results indicate that while the elastic behavior of the aortic wall is globally anisotropic, there exists a state of deformation at which the vessel displays incremental isotropy. This state of deformation corresponds approximately to the loading conditions to which the aorta is exposed in situ. Values of the moduli, analyzed as a function of transmural pressure, show that the stiffness of the aortic wall is fairly constant at low pressures but raises steeply for pressures higher than physiological. For axial stretches as occurring in situ, the magnitudes of the circumferential and radial moduli do not differ significantly for the thoracic aorta; hence this vessel can be regarded as transversely isotropic over a wide range of pressures. The same observation is valid also for the abdominal aorta when pressures equal or smaller than physiological are considered. For both the thoracic and abdominal segments of the aorta, the circumferential and radial moduli are smaller than the axial modulus at low pressures, while the reverse is true for large pressures. 相似文献
5.
Isolated tracheal segments were studied in vitro to determine how inflation affects the length and tension of the contracted and relaxed trachealis muscle. Circumferential trachealis muscle lengths were measured from cross-sectional radiographs taken during stepwise inflation of intact 20-cm-long tracheal segments to an inflation pressure of 25 cmH2O. A tracheal length spanning two cartilage rings was then cut out and mounted in a tissue bath using clips attached at the points of muscle insertion into the cartilage. The ring was stretched open along the axis of the muscle, and the resulting forces of the relaxed and contracted muscle and the cartilage were measured. Muscle lengths and tensions during inflation of the trachea were determined by comparing pressure vs. length and force vs. length measurements. During inflation from 0 to 25 cmH2O, the circumferential length of the trachealis muscle contracted with 10(-5) M acetylcholine increased from 48 to 70% of its length of maximal active tension (Lmax), while the relaxed muscle increased from 80 to 93% Lmax. The length of the contracted muscle was maintained at a nearly constant proportion of its relaxed length at each pressure. 相似文献
6.
The effects of a synthetic form of Atrial Natriuretic Factor (ANF) on spontaneously hypertensive rat aortic smooth muscle were investigated using either an alpha-adrenoceptive agonist (phenylephrine) or an agent which partially depolarized the plasma membrane (20mM KCl) as a contractile agent. The relaxant response was studied under conditions resembling normal physiological calcium ion levels (1.5mM) as well as over a range of calcium ion concentrations (0.1-2.5mM). The results demonstrate a hyporesponsiveness of hypertensive aorta to vasorelaxation induced by synthetic ANF, which is more apparent when the tissue is contracted with KCl. The results also suggest that ANF, which has been shown previously to inhibit intracellular and receptor operated calcium channel mobilization only, may additionally work through a mechanism which is related to the voltage induced calcium flux across the membrane, which also is inhibited less in hypertensive smooth muscle. 相似文献
7.
Abdominal aortic coarctation above the renal arteries leads to severe hypertension above the stenotic site and provides a model for simultaneous testing of the effects of increased and decreased pressure and consequently shear stress in the same animal. The effects of increased pressure, per se, on oxidative stress and antioxidant enzyme expression is unknown. We studied the protein expressions of antioxidant enzymes and NADPH oxidase (gp91phox subunit) in the aortic segments above and below the stenosis site in sham-operated control and aortic-banded rats at four weeks postoperatively. Compared with the control group, the banded group showed significant up-regulation of NADPH oxidase, catalase (CAT), Cu/Zn superoxide dismutase (SOD) and Mn SOD protein content in the thoracic aorta. In contrast, Mn SOD, Cu/Zn SOD and NADPH oxidase protein abundance were unchanged in the abdominal aortic segment below the stricture where blood pressure is not elevated, whereas CAT protein abundance was also elevated in the abdominal aorta. No changes were noted for glutathione peroxidase (GPX) protein content either in the thoracic or abdominal aortic segments. Coarctation-induced hypertension is associated with increased aortic CAT, Cu/Zn SOD, Mn SOD and NADPH oxidase protein expression. The up-regulation of NADPH oxidase increases reactive oxygen species (ROS) generation noted in the present study and contributes to inactivation of nitric oxide (NO) as shown previously in this model. Upregulation of antioxidant enzymes may be a compensatory response in the face of elevated pressure and oxidative stress. The normality of protein abundance in the abdominal aorta wherein blood pressure is not elevated points to the role of baromechanical factors, as opposed to circulating humoral factors that were similar in both segments, as a mechanism responsible for increased antioxidant enzyme expression. 相似文献
8.
Increased arterial load alters aortic structural and functional properties during embryogenesis 总被引:1,自引:0,他引:1
Lucitti JL Visconti R Novak J Keller BB 《American journal of physiology. Heart and circulatory physiology》2006,291(4):H1919-H1926
As in the adult dorsal aorta, the embryonic dorsal aorta is an important determinant of cardiovascular function, and increased stiffness may have secondary effects on cardiac and microcirculatory development. We previously showed that acutely and chronically increased arterial load via vitelline artery ligation (VAL) increases systemic arterial stiffness. To test the hypothesis that local dorsal aortic stiffness also increases, we measured aortic pulse-wave velocity (PWV) and assessed the active and passive properties (stress and strain) of isolated aortic segments. PWV along the dorsal aorta increased acutely and chronically after VAL. Analysis of isolated aortic active properties suggests that load-exposed aortas experienced higher stress, but not strain, at similar intraluminal pressures. When smooth muscle tone was relaxed, strain decreased in VAL vessels, whereas stress became similar to control vessels. Immunohistochemical analysis revealed that although aortic smooth muscle alpha-actin content was similar between groups, more cell layers expressed smooth muscle alpha-actin, and myocyte cell shape was markedly rounder in VAL embryos. Additionally, aortic and perivascular collagen type I and III content significantly increased in load-exposed VAL vessels. Increased production of these proteins is consistent with the observed increase in aortic PWV and decreased strain in VAL passive aortic segments. Thus the embryonic dorsal aorta is sensitive to increased arterial load and adapts by altering its material properties via changes in collagen content. 相似文献
9.
Summary Smooth muscle cells were identified in aortic cultures from which the explants had not been removed. They appeared in cultures from intact aorta within 10 days and in cultures from aorta with plaques in 15 days. Myometrial smooth muscle cells grew within 2–4 days.Differences in growth rate of smooth muscle cells from various sources were explained as were differences in growth of aortic and subcutaneous fibroblasts. Morphologic characteristics of smooth muscle cells and fibroblasts were described in details. Peculiarities of migration of smooth muscle cells in vitro were discussed.This study was supported by grant HE-02534 from the National Heart Institute. 相似文献
10.
Victoria P. Le Yoshito Yamashiro Hiromi Yanagisawa Jessica E. Wagenseil 《Biomechanics and modeling in mechanobiology》2014,13(5):1081-1095
Mice with a smooth muscle cell (SMC)-specific deletion of Fibulin-4 (SMKO) show decreased expression of SMC contractile genes, decreased circumferential compliance, and develop aneurysms in the ascending aorta. Neonatal administration of drugs that inhibit the angiotensin II pathway encourages the expression of contractile genes and prevents aneurysm development, but does not increase compliance in SMKO aorta. We hypothesized that multidimensional mechanical changes in the aorta and/or other elastic arteries may contribute to aneurysm pathophysiology. We found that the SMKO ascending aorta and carotid artery showed mechanical changes in the axial direction. These changes were not reversed by angiotensin II inhibitors, hence reversing the axial changes is not required for aneurysm prevention. Mechanical changes in the circumferential direction were specific to the ascending aorta; therefore, mechanical changes in the carotid do not contribute to aortic aneurysm development. We also hypothesized that a published model of postnatal aortic growth and remodeling could be used to investigate mechanisms behind the changes in SMKO aorta and aneurysm development over time. Dimensions and mechanical behavior of adult SMKO aorta were reproduced by the model after modifying the initial component material constants and the aortic dilation with each postnatal time step. The model links biological observations to specific mechanical responses in aneurysm development and treatment. 相似文献
11.
In this paper, we studied the viscoelastic behaviors of isolated aortic elastin using combined modeling and experimental approaches.
Biaxial stress relaxation and creep experiments were performed to study the time-dependent behavior of elastin. Experimental
results reveal that stress relaxation preconditioning is necessary in order to obtain repeatable stress relaxation responses.
Elastin exhibits less stress relaxation than intact or decellularized aorta. The rate of stress relaxation of intact and decellularized
aorta is linearly dependent on the initial stress levels. The rate of stress relaxation for elastin increases linearly at
stress levels below about 60 kPa; however, the rate changes very slightly at higher initial stress levels. Experimental results
also show that creep response is negligible for elastin, and the intact or decellularized aorta. A quasi-linear viscoelasticity
model was incorporated into a statistical mechanics based eight-chain microstructural model at the fiber level to simulate
the orthotropic viscoelastic behavior of elastin. A user material subroutine was developed for finite element analysis. Results
demonstrate that this model is suitable to capture both the orthotropic hyperelasticity and viscoelasticity of elastin. 相似文献
12.
Yoshiki Takehara Hiroko Nakahara Shohei Okada Kiyonori Yamaoka Keisuke Hamazaki Akihiro Yamazato 《Free radical research》2013,47(4):287-294
Nitric oxide (NO) functions as an endothelium-derived relaxation factor and regulates vascular resistance. Recent studies in this laboratory (Arch. Biochem. Biophys. 323, 27–32, 1995) revealed that the lifetime of NO significantly increased at physiologically low levels of oxygen concentrations and, hence, this gaseous radical strongly inhibited mitochondrial electron transport for a fairly long duration at low oxygen concentrations. The present work describes the effect of oxygen concentration on NO-induced relaxation and guanylate cyclase (GC) activity of endothelium-denuded aorta of the rat. Both NO and 2,2′-hydroxynitrosohydrazono)bis-ethanamine (NOC18), an NO donor, induced the relaxa-tion of endothelium-denuded helical segments of rat aorta which were contracted by norepinephrine. NO-dependent relaxation of arterial specimens was enhanced by lowering oxygen concentration in the medium with concomitant increase in their cGMP levels. Anoxia induced the relaxation of the aorta by some NO-enhanceable and methylene blue-insensitive mechanism. These results suggested that local concentrations of oxygen might play important roles in the regulation of NO-dependent GC activity and vascular tonus of resistance arteries. 相似文献
13.
Julia T. Apter 《Bulletin of mathematical biology》1964,26(4):367-388
Visco-elastic properties of blood vessels, the aorta in particular, may be approximated by a suitable arrangement of two elastic
rods and one viscous rod. This simple model is readily derived from models already proposed for individual components of the
aortic wall: elastin, collagen, and smooth muscle. The model is even consistent with various results ofin vitro experimental data which have previously appeared uncorrelated, or even contradictory. What is more, when elastic and viscous
coefficients of the model were calculated from these data, the density of each of the three histological components could
be predicted for some specimens. The mathematical development of the model and the agreement with varied experiments justify
use of the model for analysis ofin vivo aortic pressure curves in subsequent papers. The development also indicates the need for additional data from some simplein vitro experiments suggested by this analysis. Data from the suggested experiments may support the present model or require some
modification of it. 相似文献
14.
Freeman W. Cope 《Bulletin of mathematical biology》1960,22(1):19-40
A review of the literature of aortic elasticity data leads to the conclusion that the pressure-volume curves of the human
aorta can be reasonably well approximated by parabolas, while a straight line approximation proves inadequate. By using the
above relationship and assuming flow through the peripheral vascular system to be directly proportional to the pressure difference
across it, two equations are derived which relate systolic and diastolic blood pressures, cardiac output, pulse rate, peripheral
resistance, and two constants of aortic elasticity. Pulsewave transmission phenomena are ignored. These two equations can
be solved simultaneously for any two variables in terms of the others. When values computed from these equations are compared
with experiment, the errors are usually less than 5 per cent, provided errors due to inaccurate measurements of the aortic
elasticity constants are avoided. 相似文献
15.
John H Lillvis Robert Erdman Charles M Schworer Alicia Golden Kimberly Derr Zoran Gatalica Laura A Cox Jianbin Shen Richard S Vander Heide Guy M Lenk Leigh Hlavaty Li Li James R Elmore David P Franklin John L Gray Robert P Garvin David J Carey Wayne D Lancaster Gerard Tromp Helena Kuivaniemi 《BMC physiology》2011,11(1):1-11
16.
The aorta is a magistral artery, which has been traditionally looked upon as a vessel whose properties are invariable throughout its length. However, in the most recent decade, there have been accumulated data that provide evidence that different aorta sections arise from different embryonic origins and that the population of smooth muscle cells making up the vessel’s wall is, consequently, heterogenic. Tracing the fate of smooth muscle cells, the basic components of the vessel, with the aid of genetic marking methods revealed that the cells’ response to various factors is largely determined by the embryonic origin of a certain cell population. However, functional differences between the smooth muscle cells making up different aorta sections remain poorly understood. The aim of the current work was to compare the functional characteristics of the populations of aortic wall smooth muscle cells obtained from the aorta sections differing by their embryonic origin. Towards this end, we obtained smooth muscle cell cultures from the three aorta sections of linear rats, namely, the neural crest derived ascending thoracic aorta, the somites derived descending thoracic aorta, and splanchnic mesoderm derived abdominal aorta. Using immunocytochemistry and Western blotting, the cells from the different regions of aorta were compared on the basis of smooth muscle actin, vimentin, and SM22 content in them. Cell proliferation rate was estimated using the growth curves method. We have demonstrated that the three smooth muscle cell populations arising from different embryonic origins differ in their morphological characteristics as well as by smooth muscle actin and SM22 content. We have shown that smooth muscle cells from the ascending aorta proliferate more actively than the corresponding cells from the descending thoracic aorta. Thus, the functional properties of the populations of rat aortic smooth muscle cells are different and depend on the embryonic origin of the aorta section from which they were obtained. 相似文献
17.
Blanca Mompeó Lilian Pérez Antonio Fernández Pedro Saavedra Miguel Rivero Manuel Arbelo Marina Arregui Cristian Suárez-Santana Yara Bernaldo-de-Quiros 《Journal of morphology》2020,281(3):377-387
Some modifications in the vascular system of marine mammals provide adaptive advantages for diving. This study analyses the organisation of the aortic wall in dolphins, observing artery changes in volume and blood pressure for diving behaviour. Samples of three aortic segments (ascending, thoracic and abdominal) of three dolphin species were processed for histological and morphometric studies. The three dolphin species used, striped dolphin (Stenella coeruleoalba), Atlantic spotted dolphin (Stenella frontalis) and common dolphin (Delphinus delphis), have shallow or intermediate diving habits. Our results indicated that the components of the aortic wall of the dolphins had different dispositions in the three selected segments. The aortic wall decreased in thickness along its length due to a loss of the lamellar units in the tunica media and a thinning of the main elements of the lamellar units along the artery. The life stage had little influence on the thickness of the aortic wall except for the ascending aorta. The weight, body length, species or sex of the specimen did not significantly influence the thickness of the wall or the lamellar units. In summary, the histological and morphometric aortic structure in dolphins, in relation to the studied parameters, seems to be similar to that previously described of terrestrial mammals such as pigs, except for a larger difference in the proportion of lamellar units between the ascending and thoracic segments. 相似文献
18.
The pressure dependent dynamic elasticity of 35 thoracic and 16 abdominal human aortas in vitro described by a five component model 总被引:3,自引:0,他引:3
Segments of 35 thoracic and 16 abdominal human aortas, including nine pairs, aged 30-78 yr at autopsy, were perfused with 37 degrees C Tyrode's solution at in situ length. Diameter changes due to 20 mmHg pressure steps between 20 and 180 mmHg were measured to 1 micron accuracy at an equivalent noise level of 0.1 micron RMS, using balanced transducers. Aortic creep curves at each pressure level were described individually by a constant plus bi-exponential creep model characterized by two creep fractions (alpha 1 and alpha 2) and two time constants (tau 1 and tau 2). Creep fractions and time constants increased substantially with the pressure level, indicating a significant effect of pressure or distension on aortic viscoelasticity. At 110 mmHg the mean +/- 1 S.D. parameter values were: thoracic aorta: alpha 1 = 0.076 +/- 0.017, alpha 2 = 0.102 +/- 0.028, tau 1 = 0.73 +/- 0.29 s, tau 2 = 14.0 +/- 4.1 s; abdominal aorta: alpha 1 = 0.078 +/- 0.017, alpha 2 = 0.101 +/- 0.025, tau 1 = 0.61 +/- 0.12 s, tau 2 = 12.1 +/- 3.4 s. Nine paired comparisons at each pressure level showed that creep fractions and time constants of thoracic and abdominal segments were not significantly different (p = 0.05). 相似文献
19.
E. S. J. Kr?ner J. J. M. Westenberg L. J. M. Kroft N. J. Brouwer P. J. van den Boogaard A. J. H. A. Scholte 《Netherlands heart journal》2015,23(10):493-501
Aims
Thoracic aortic aneurysm (TAA) is potentially life-threatening and requires close follow-up to prevent aortic dissection. Aortic stiffness and size are considered to be coupled. Regional aortic stiffness in patients with TAA is unknown. We aimed to evaluate coupling between regional pulse wave velocity (PWV), a marker of vascular stiffness, and aortic diameter in TAA patients.Methods
In 40 TAA patients (59 ± 13 years, 28 male), regional aortic diameters and regional PWV were assessed by 1.5 T MRI. The incidence of increased diameter and PWV were determined for five aortic segments (S1, ascending aorta; S2, aortic arch; S3, thoracic descending aorta; S4, suprarenal and S5, infrarenal abdominal aorta). In addition, coupling between regional PWV testing and aortic dilatation was evaluated and specificity and sensitivity were assessed.Results
Aortic diameter was 44 ± 5 mm for the aortic root and 39 ± 5 mm for the ascending aorta. PWV was increased in 36 (19 %) aortic segments. Aortic diameter was increased in 28 (14 %) segments. Specificity of regional PWV testing for the prediction of increased regional diameter was ≥ 84 % in the descending thoracic to abdominal aorta and ≥ 68 % in the ascending aorta and aortic arch.Conclusion
Normal regional PWV is related to absence of increased diameter, with high specificity in the descending thoracic to abdominal aorta and moderate results in the ascending aorta and aortic arch. 相似文献20.
Julia T. Apter 《Bulletin of mathematical biology》1963,25(3):325-339
Aortic diastolic pressure curves calculated on the basis of a constant peripheral resistance do not predict humps like those
found in actual clinical records. The present study considers the form of the aortic pressure curve to be expected during
diastole if blood flowed out of the aorta against a peripheral resistance (R) that changed in response to baroreceptor stimulation. Assumptions are (1) baroreceptor stimulation is provided by arterial
pressures above a threshold pressureP
*; (2) pressures aboveP
* occur during late systole and early diastole; (3) peripheral resistance starts to respond a given time interval Δ afterP reachesP
* in systole; (4) Δ is less than the duration of time betweenP
* occurring during early diastole and the end of the cardiac cycle. The present analysis predicts humps in the diastolic pressure
curve, the humps occurring at times when the peripheral resistance time course alters. Initially,R is considered constant at its maximal valueR
0, then it changes in response to pressures during systole (assumed to have a parabolic time course), then changes in response
to pressures during early diastole (assumed to have an exponential time course), and then changes in response to pressures
later in diastole. The humps so predicted occur at times consistent with the location of humps in clinical records. What is
more, this analysis predicts no humps in association with an unresponsive peripheral resistance as in essential hypertension,
in agreement with clinical findings. 相似文献