Holarctic phylogeography of an asexual species complex – II. Allozymic variation and clonal structure in Arctic Daphnia

Previous mitochondrial (mt)DNA sequencing and restriction fragment length polymorphism (RFLP) studies have shown that the Holarctic Daphnia pulex complex is divisible into two major groups (pulicaria and tenebrosa) that exhibit distinct phylogeographic patterns. Here we examine allozymic variation at six polymorphic enzyme loci to ascertain clonal structure and clonal distribution patterns within each group. Specimens were collected from a total of 850 populations encompassing the Arctic. A significant negative relationship (Mantel test) between similarity of regional clonal arrays and geographical distance was observed. A small fraction of clones in each group was widespread (in the order of 1000s of kilometres). However, most clones were restricted to single regions, and were often found only in a single population. These data indicate that the population genetic structure is highly fragmented in this complex, but the potential for long-distance passive dispersal exists. Further, ‘hot spots’ of high clonal richness and diversity were found in each group, which is concordant with earlier work. In addition, ≈ 20% of pulicaria group clones possess nuclear genes from tenebrosa, while approximately 10% of tenebrosa group clones harbour pulicaria nuclear genes. These data indicate nuclear introgression between the two groups, which was found to be prominent in a broad zone of secondary contact encompassing parts of northwestern Russia, northern Fennoscandia, Svalbard, and extending into the high eastern Canadian Arctic.     

Length–weight and length–length relationships of fish species from the Chi River,northeastern Thailand

The relationships of total length (TL), fork length (FL) and standard length (SL) are presented for four fish species and the relationships between TL and wet weight for 23 fish species from the Chi River in northeastern Thailand. All length–length relationships were linear (r² > 0.95). Slope (b) of the length–weight relationships showed values between 2.5073 and 3.4896.     

Size at first sexual maturity,fecundity, length–weight and length–length relationships of Puntius sophore (Cyprinidae) in Bangladeshi waters

The present study describes the size at first sexual maturity, fecundity, length–weight (LWRs) and length–length relationships (LLRs) of the pool barb, Puntius sophore, using data obtained from different geographical locations in Bangladesh. A total of 905 specimens were caught by traditional fishing gear from March 2010 to February 2011. Additionally, a total of 121 females were collected from a commercial catch of the Padma River during June–July 2011 to estimate size at first maturity and to determine fecundity. Total length (TL), fork length (FL) and standard length (SL) were measured with digital slide calipers. Individual body weights (BW) were determined for all specimens, and gonad weights (GW) from 121 females were weighed to an accuracy of 0.001 g. The female gonadosomatic index (GSI) was calculated as [GSI (%) = (GW/BW) × 100]. Female size at first maturity was estimated using GSI and TL as indicators, and estimated as 5.00 cm TL in the Padma River. Specimens larger than 5.00 cm TL were used to determine fecundity. Mean total fecundity was 5300 ± 2700, ranging from 1580 to 16590. A positive exponential correlation was recorded between total fecundity and total length (r² = 0.421). Relative fecundity ranged from 466 to 4036 (mean 1100 ± 580) in the Padma River. The LWR of pooled data for sexes combined was estimated as BW = 0.0155 TL^2.98 as ancova revealed no significant differences in LWRs between rivers (P > 0.05). All LLRs were highly correlated (r² > 0.983; P < 0.001), and ancova analyses further indicated that LLRs did not differ between rivers (P > 0.05). These results will help in further studies on the population assessment of the species.     

Length–weight and length–length relationships of seven freshwater fish species from the Daning River,a tributary of the Yangtze River,southwest China

This study determined the length–weight relationships (LWRs) and length–length relationships (LLRs) of seven freshwater fish from the Daning River, a tributary of the Yangtze River, southwest China. Specimens were collected between July 2016 and July 2017 using drift gill nets (mesh size 1, 2 and 3 cm), cage net (0.5 cm mesh size) and electroshock fishing techniques quaterly. Parameter b values of LWRs ranged from 2.647 to 3.276 (r² > .963) and b values of LLRs ranged from 1.091 to 1.213(r² > .985). Six new LLRs and seven new maximum length data are presented. The biometric data and their relationships are relevant to Fishers science to supplement other data sets needed for fisheries management.     

Growth and length–weight relationship of the juvenile anchovy, Engraulis encrasicolus, in the nursery ground (Zrmanja River estuary–eastern Adriatic Sea)

Growth and length–weight relationships of 2564 juvenile specimens of anchovy, Engraulis encrasicolus (Linnaeus, 1758), were examined over a 2‐year period (August 1989 to April 1991) in the Zrmanja River estuary. Fish sizes ranged from 4.4 to 12.5 cm total length and 0.5 and 13.8 g, corresponding to age (in years) 0+ (98%) and 1+ (2%). Length–weight regression coefficient (b) varied between 2.95 and 3.42 and the regression constant (a) between 2.1 and 6.0 (×10^?3). The von Bertalanffy growth equation of juvenile anchovy was: l_t = 13.2 (1 ? e^?0.82(t+0.5)). Maturity was reached in June in males above 8.2 cm and females above 9.0 cm total length.     

Length–weight relationship and condition factor of the Characidae matrinxã, Brycon falcatus (Müller & Troschel, 1844), in the Teles Pires River,southern Amazon

The aim of this study was to verify whether the in situ supply of soybean as a fish attractant bears any influence on the length–weight relationship and condition factor of the omnivorous fish species, Brycon falcatus. In addition, estimated was the length–weight relationship, standard and total length, condition factor, and size of B. falcatus in the spawning period in the Teles Pires River Basin. In the experimental design, rivers of the Teles Pires Basin containing soybean attractant (Verde River, Celeste River, Teles Pires River and the Tapaiuna River) were mapped. Fish were also collected from the Cristalino River, which contained no soybean attractant (treatment control). Samples were collected from August 2012 to July 2013. Length–weight relationship of B. falcatus in the Teles Pires River Basin can be obtained by the expression: Wt = 0.0091*SL^3.376, r² = .97, n = 102, and length‐length relationship: TL = 1.128*SL + 3.978, r² = .96. The condition factor of all specimens collected in the Teles Pires Basin was k = 1.055. Size at capable phase (L50) of spawning was estimated at 23 cm standard length. Results showed no significant difference in the length–weight relationship or condition factor of B. falcatus collected from rivers with or without soybean attractant. Concluded is that the in situ supply of soybean as a fish attractant does not interfere with the wellbeing of B. Falcatus in the Teles Pires River Basin.     

Length–weight relationships of two fish species from the middle reaches of the Jinsha River,China

This study provides the first report of length–weight relationships (LWRs) for Pseudogyrinocheilus prochilus (Sauvage & Dabry de Thiersant, 1874) and Jinshaia sinensis (Sauvage & Dabry de Thiersant, 1874) collected from the middle reaches of the Jinsha River, southwest China. Samples were obtained between June 2005 and November 2006. A total of 491 specimens belonging to two fish species were analyzed. LWRs were W = 0.0170L^2.970 (r² = 0.980) for P. prochilus and W = 0.0194L^2.849 (r² = 0.977) for J. sinensis. Prior to this study, the LWRs for P. prochilus were unknown. In addition, new maximum size for J. sinensis was provided.     

Length–weight and length–length relationships of four fish species from the middle reaches of the Minjiang River,southwest China

Length–weight (LWR) and length–length (LLR) relationships are reported for Glyptothorax fokiensis (Rendahl, 1925), Microphysogobio kachekensis (Oshima, 1926), Pseudobagrus brevicaudatus (Wu, 1930), and Sinibrama taeniatus (Nichols, 1941) from the middle reaches of the Minjiang River and its tributaries, Sichuan Province, in southwest China. A total of 675 specimens were obtained between 2013 and 2016 using various types of fishing gear and electroshock fishing techniques. Standard length and total length for each individual were measured by digital slide caliper and each body weight was taken by digital balance. The b‐values for LWRs varied from 2.617 to 3.249 (r² > .954), and the LLRs were highly correlated (r² > .983; p < .01).     

Length–weight,length–length relationships and feeding habits of wild Indus Mahseer,Tor macrolepis,from Attock,Pakistan

In this study the length–weight (LWR) and length–length relationships (LLR) were carried out for Indus Mahseer, Tor macrolepis, from the Haro River and Nalah Kala, Attock, Pakistan. A total of 118 specimens were used to estimate the relationship parameters. The results of LWR (W = a TL^b) from five different sites indicated W = 0.0044 TL^3.14, W = 0.0054 TL^3.08, W = 0.0059 TL^3.21, W = 0.0076 TL^3.12, and W = 0.0107 TL^2.99, respectively, with an overall value of W = 0.0100 TL^2.94. All regressions for LLR were highly significant (P ≤ 0.001), with coefficient of determination (r²) values of > 0.90. All relationships for LWR and LLR were found to be isometric. T. macrolepis is principally a herbivorous bottom feeder, mainly feeding on aquatic plants and algae, supplemented by insects.     

Length‐weight and length–length relationships of three algae‐scraping cyprinid species from Iran

The study presents length–weight relationships (LWRs) and length–length relationships (LLRs) for three algae‐scraper species; Capoeta coadi from the Kouhrang River (Iran; 32°23′N, 50°08′E) a part of the Tigris drainage basin, Capoeta pyragyi from Cheshmeh Langan River (32°55′N, 50°10′E) a part of Zayanderud River basin and Capoeta umbla from Chooman River (35°57′N, 45°47′E). Fish were sampled by electro fishing (Samus1000; Power Out: 200 watts) in August, September and October 2017. Sampling was carried out each months in sites covering an area of about 100 m² including runs, riffles and pools at a water depth of 10–60 cm. The obtained LWRs and LLRs showed a high level of correlation (r² > 0.99).     

Length–weight and length–length relationships for eight fish species from the Jarrahi River,southwestern Iran

Length–weight and length–length relationships were estimated for eight fish species belonging to three families from the Jarrahi River in southwestern Iran. The study was performed between June 2009 and September 2011. Weight–length relationships were estimated using the logarithmic form of the equation W = aL^b. The b values varied between 2.96 and 3.61 and determined as 3.42 for Alburnus mossulensis Heckel, 1843; 3.33 for Alburnoides idignensis Bogutskaya and Coad, 2009; 2.85 for Barbus grypus Heckel, 1843; 3.61 for Barilius mesopotamicus Berg, 1932; 3.16 for Cyprinion kais (Heckel, 1843); 3.36 for Cyprinion macrostomum Heckel, 1843; 3.27 for Paraschistura nielseni (Nalbant and Bianco, 1998); and 2.96 for Glyptothorax silviae Coad, 1981. This study presents the first LWR and LLR references for these species in the Jarrahi River.     

Length–weight relationships for three fish species from Yalong River in China

This study provides the first report of length–weight relationships (LWRs) for three fish species collected from the Yalong River, one of the tributaries to the upper reaches of the Yangtze River, China. The LWRs of these fish species were BW = 0.0070TL^3.0539 (r² = 0.9662) for Schizothorax wangchiachii, BW=0.0134TL^2.8523 (r² = 0.9676) for Schizopygopsis malacanthus, and BW=0.0143TL^2.9253 (r² = 0.8538) for Euchiloglanis kishinouyei, respectively. Also investigated were the spawning season, absolute fecundity and main food items of the three fish species in the natural environment. In addition, new maximum sizes for the three species are provided.     

Length–weight and length–length relationships for seven fish species from the Zohreh River,Iran

Length–weight and length–length parameters were calculated for seven fish species belonging to five families in the Zohreh River, Iran. The weight–length relationships were estimated using the equation W = aL^b. The b values varied between 2.72 and 3.33, with a mean ± SD of 2.88 ± 0.28. The b values parameter in the length–weight relationship equations were calculated as 3.15 for Alburnus mossulensis Heckel, 1843; 3.78 for Barilius mesopotamicus Berg, 1932; 2.86 for Cyprinion macrostomum Heckel, 1843; 2.89 for Nuchequula gerreoides (Bleeker, 1851); 2.80 for Mastacembelus mastacembelus (Banks & Solander, 1794); 2.72 for Liza abu (Heckel, 1843); and 3.19 for Paraschistura nielseni (Nalbant and Bianco, 1998). This study presents the first LWR and LLR references for these species in the Zohreh River.     

Length–weight relationships of four fish species from the upper Paraná River basin,Southeastern Brazil

Here the length–weight relationships (LWR, hereafter) of Pareiorhina pelicicei, Trichomycterus candidus, T. pirabitira, and T. piratymbara are provided. Samples of each species were collected between April and July 2017 with a hand net (1.5 mm mesh) in three sites from the upper Paraná River basin, Southeastern Brazil. These results increase knowledge about the LWR of Neotropical freshwater fish.     

Length‐weight and length‐length relationships of two gobiid fishes Eleotris fusca (Forster, 1801) and Odontamblyopus rubicundus (Hamilton, 1822) from the Payra River,southern Bangladesh

The present study provides the estimates of length‐weight relationships (LWRs) and length‐length relationships (LLRs) of two gobiid fishes Eleotris fusca and Odontamblyopus rubicundus from the Payra River, southern Bangladesh. Fish samples were collected from the set bagnet (mesh size <2 mm) fishers of the Payra River in July 2016. Allometric coefficient (b) values were 2.95 for E. fusca and 2.76 for O. rubicundus. Additionally, the LLRs were highly significant with r² ≥ .955. While the estimate of the LWR for O. rubicundus includes a substantial larger sample size and thus provides a more reliable LWR than the one published so far, the LWR for E. fusca must be considered as tentative because the sample includes only juveniles for a fish that reaches a maximum standard length more than three times as much. Additional studies are needed to further improve our LWR knowledge base for these fish species.     

Length‐weight and length‐length relationships of four small fishes from the Atrai River,Dinajpur, Bangladesh

Length‐weight (LWRs) and length‐length relationships (LLRs) were determined for four fish species collected from the Atari River in Dinajpur of Bangladesh. Sampling took place monthly between January and June 2016, using seine nets of commercial fishermen (mesh size 4 mm). In LWRs (r² > .927, p < .05), slope (b) was estimated as 3.191, 2.992, 3.217 and 3.109 for Acanthocobitis botia, Botia lohachata, Canthophrys gongota and Chanda nama, respectively. In LLRs (r² > .812, p < .05), lengths i.e. TL, SL, HL and FL were highly correlated. The present study on these species would be the baseline for FishBase dataset.     

Length‐weight relationships of three freshwater fish species from the Nandu River and Changhua River in Hainan Island,China

Length‐weight relationships (LRWs) were determined for three fish species from the Hainan Island, China. Fish specimens were sampled across the Nandu River and Changhua River from May 2017 to June 2018 with benthic fyke‐nets (stretched mesh size: 0.4 cm), multi‐mesh gillnets (stretched mesh size ranging from 1.0 to 12.5 cm) and electrofishing. Relationships between length and weight were fitted very well for all species (all R²>0.95). The b values of the LRW equations ranging from 2.938 for Liniparhomaloptera qiongzhongensis to 3.259 for Toxabramis houdemeri.     

Length–weight relationships of five fish species from the Beijiang River,China

The length–weight relationships (LWRs) were determined for five fish species from the Beijiang River in China. Fishes were caught by gill nets (mesh sizes: 5, 7, 9 cm), and cage nets (mesh sizes: 0.5 cm) from April to September 2018. Length–weight relationships of four species were unknown to FishBase, and new records of maximum lengths are given for two species, one of which, Ancherythroculter lini, is endemic species of the Pearl River. All length–weight relationships were highly significant on a log scale with the coefficient of determination (r²) values ranged from 0.939 to 0.991. Values of b were within the expected range, varied from 2.935 for A. lini to 3.414 for Parasinilabeo assimilis.     

Length–weight relationships for four endemic fish species from the Yalu River,China

Length–weight relationships (LWRs) are presented for four endemic fish species caught in the upper Yalu River during the yearly surveys (2017) by either electrofishing or fish cage net (5 mm mesh size) as soon as the ice melted. The parameter b ranged between 2.244 in Gobio macrocephalus and 3.428 in Ladislavia taczanowskii. Additionally, the parameter a ranged between 0.004 in Misgurnus bipartitus and 0.059 in Gobio macrocephalus. All length‐weight relationships were highly significant (r² ≥ 0.8754) on a log scale.     

Length–weight and length–length relationships of 26 fish species from the Yiluo River,a tributary of the Yellow River,China

From February to October 2016, a total of 9,754 fish specimens were collected from the Yiluo River, a tributary of the Yellow River, China's second longest river. Based on these samples, length–weight relationships (LWRs) and length–length relationships (LLRs) were estimated for 26 species. Among these data, LWRs for 1 species, LLRs for 11 species, and maximum total lengths for six species were not previously recorded in FishBase. The a values of the LWRs varied between 0.0019 and 0.0116, and b values from 2.805 to 3.883. All regressions for LWRs and LLRs were highly significant (p < .001). These results will be useful for sustainable management and conservation of the fish resources in the Yiluo River and the Yellow River ecosystem.