共查询到20条相似文献,搜索用时 31 毫秒
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Overexpression of the ethylene-responsive factor gene BrERF4 from Brassica rapa increases tolerance to salt and drought in Arabidopsis plants 总被引:1,自引:0,他引:1
Yean Joo Seo Jong-Beum Park Yeon-Jeong Cho Choonkyun Jung Hak Soo Seo Soon-Ki Park Baek Hie Nahm Jong Tae Song 《Molecules and cells》2010,30(3):271-277
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Management of Pacific halibut (Hippoglossus stenolepis), a long-lived flatfish, is complicated by possible ontogenic and sex-specific variation in migration. Archival tags promise
the ability to help uncover long-term movement patterns at the individual level, if the tags can be retained and recovered
from healthy fish. We examined fifteen individuals (69–90 cm fork length) for long-term physiological response to intracoelomic
implantation of three types of archival tags: fully internal, internal with right angle protruding light stalk, and internal
with straight protruding light stalk. Tags represented 0.05–0.16% of initial fish weights. Fish were reared at 10.8 ± 1.1°C
for 59 weeks post-surgery. One fish died after 39 weeks from thermal stress unrelated to the surgical procedure. Temporal
variation in behavior of tagged fish was indistinguishable from that of controls (n = 15 tagged, 5 controls). Treatment and control-group fish grew at similar rates. No tag expulsion or physiological response
was evident in the individual that died at 39 weeks, but nine of eleven individuals dissected at the end of 59 weeks had developed
internal responses. These responses ranged from deposition of fibrous protein and/or calcitic material on tag surfaces to
partial or full tag encapsulation in either the visceral peritoneal layer (fully-internal tags) or the intestinal mesenteries
(stalk-bearing tags). The responses were within the range reported for other pleuronectids implanted with tags of similar
configuration and may have implications for design and interpretation of long-term tagging studies. Encapsulation may reduce
the probability of tag recoveries even in the absence of tag expulsion, especially in species eviscerated at sea. 相似文献
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S. H. Luijten N. S. Schidlo P. G. Meirmans T. J. de Jong 《Plant biology (Stuttgart, Germany)》2015,17(1):262-267
We used flow cytometry, chromosome counting and AFLP markers to investigate gene flow from the crop plant oilseed rape, Brassica napus (AACC) to wild B. rapa (AA) in the Netherlands. From 89 B. napus source populations investigated, all near cropping fields or at transhipment sites, only 19 contained a B. rapa population within a 2.5‐km radius. During our survey we found only three populations with F1 hybrids (AAC), as recognized by their nine extra chromosomes and by flow cytometry. These hybrids were all collected in mixed populations where the two species grew in close proximity. Populations with F1 hybrids were not close to crops, but instead were located on road verges with highly disturbed soils, in which both species were probably recruited from the soil seed bank. Many plants in the F2, BC1 or higher backcrosses are expected to carry one to eight C chromosomes. However, these plants were not observed among the hybrids. We further investigated introgression with molecular markers (AFLP) and compared sympatric B. rapa populations (near populations of B. napus) with control populations of B. rapa (no B. napus within at least 7 km). We found no difference between sympatric and control populations in the number of C markers in B. rapa, nor did we find that these sympatric populations closely resembled B. napus. Our data show that hybrids occur but also suggest no recent introgression of alleles from the crop plant B. napus into wild B. rapa in the Dutch populations studied. 相似文献
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Ribosome profiling reveals dynamic translational landscape in maize seedlings under drought stress
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Lei Lei Junpeng Shi Jian Chen Mei Zhang Silong Sun Shaojun Xie Xiaojie Li Biao Zeng Lizeng Peng Andrew Hauck Haiming Zhao Weibin Song Zaifeng Fan Jinsheng Lai 《The Plant journal : for cell and molecular biology》2015,84(6):1206-1218
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Jun Young Choi Young Sam Seo Su Jin Kim Woo Taek Kim Jeong Sheop Shin 《Plant cell reports》2011,30(5):879-881
The hot pepper xyloglucan endo-trans-gluco-sylase/hydrolase (CaXTH3) gene that was inducible by a broad spectrum of abiotic stresses in hot pepper has been reported to enhance tolerance to
drought and high salinity in transgenic Arabidopsis. To assess whether CaXTH3 is a practically useful target gene for improving the stress tolerance of crop plants, we ectopically over-expressed the
full-length CaXTH3 cDNA in tomato (Solanum lycopersicum cv. Dotaerang) and found that the 35S:CaXTH3 transgenic tomato plants exhibited a markedly increased tolerance to salt and drought stresses. Transgenic tomato plants
exposed to a salt stress of 100 mM NaCl retained the chlorophyll in their leaves and showed normal root elongation. They also
remained green and unwithered following exposure to 2 weeks of dehydration. A high proportion of stomatal closures in 35S:CaXTH3 was likely to be conferred by increased cell-wall remodeling activity of CaXTH3 in guard cell, which may reduce transpirational
water loss in response to dehydration stress. Despite this increased stress tolerance, the transgenic tomato plants showed
no detectable phenotype defects, such as abnormal morphology and growth retardation, under normal growth conditions. These
results raise the possibility that CaXTH3 gene is appropriate for application in genetic engineering strategies aimed at improving abiotic stress tolerance in agriculturally
and economically valuable crop plants. 相似文献
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M. J. Hamel K. D. Steffensen J. J. Hammen M. A. Pegg 《Zeitschrift fur angewandte Ichthyologie》2013,29(1):41-43
Population augmentation and propagation of pallid sturgeon (Scaphirhynchus albus) have been a focus of scientists since the mid‐1990s. Various tag types have been used to gain a better understanding of population characteristics, stocking success, and movement patterns. Passive integrated transponder (PIT) tags have been consistently used since the inception of recovery efforts to mark age‐1 and older pallid sturgeon. This tag has been successfully used with other sturgeon species, and tagging protocols for pallid sturgeon were developed in response to those successes. Tag retention rates in pallid sturgeon have been reported as variable or unknown and there has been no formal study to determine PIT tag retention in pallid sturgeon, particularly with age‐1 individuals that are propagated in hatcheries to be stocked into the wild. The objective was to evaluate retention rates of PIT tags inserted into the operculum and along the base of the dorsal fin of age‐1 pallid sturgeon (total fork length [FL] range = 214–358 mm). Tagged sturgeon (n = 80) were held in a large holding tank and inspected for tag loss twice a month for a total of 189 days. Final tag retention was 83% for tags inserted into the operculum and 85% for tags inserted near the dorsal fin. Tags shed from the operculum were from smaller fish (mean FL = 265 mm) and losses occurred during the first 60 days of the experiment. Tags shed from the dorsal site were predominantly from larger individuals (mean FL = 305 mm) and losses were continual throughout the experiment. Future research should determine size‐specific tag retention rates so that hatcheries can maximize retention in either tag placement location. 相似文献
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Genetic changes in a novel breeding population of Brassica napus synthesized from hundreds of crosses between B. rapa and B. carinata
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Jun Zou Annaliese S. Mason Xueqi Shen Xiaohua Wang Nian Wang Fabian Grandke Meng Wang Shihao Chang Rod J. Snowdon Jinling Meng 《Plant biotechnology journal》2018,16(2):507-519
Introgression of genomic variation between and within related crop species is a significant evolutionary approach for population differentiation, genome reorganization and trait improvement. Using the Illumina Infinium Brassica 60K SNP array, we investigated genomic changes in a panel of advanced generation new‐type Brassica napus breeding lines developed from hundreds of interspecific crosses between 122 Brassica rapa and 74 Brassica carinata accessions, and compared them with representative accessions of their three parental species. The new‐type B. napus population presented rich genetic diversity and abundant novel genomic alterations, consisting of introgressions from B. rapa and B. carinata, novel allelic combinations, reconstructed linkage disequilibrium patterns and haplotype blocks, and frequent deletions and duplications (nonrandomly distributed), particularly in the C subgenome. After a much shorter, but very intensive, selection history compared to traditional B. napus, a total of 15 genomic regions with strong selective sweeps and 112 genomic regions with putative signals of selective sweeps were identified. Some of these regions were associated with important agronomic traits that were selected for during the breeding process, while others were potentially associated with restoration of genome stability and fertility after interspecific hybridization. Our results demonstrate how a novel method for population‐based crop genetic improvement can lead to rapid adaptation, restoration of genome stability and positive responses to artificial selection. 相似文献
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