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1.
QTL mapping and confirmation for tolerance of anaerobic conditions during germination derived from the rice landrace Ma-Zhan Red 总被引:3,自引:0,他引:3
Endang M. Septiningsih John Carlos I. Ignacio Pamella M. D. Sendon Darlene L. Sanchez Abdelbagi M. Ismail David J. Mackill 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(5):1357-1366
Wide adoption of direct-seeded rice practices has been hindered by poorly leveled fields, heavy rainfall and poor drainage, which cause accumulation of water in the fields shortly after sowing, leading to poor crop establishment. This is due to the inability of most rice varieties to germinate and reach the water surface under complete submergence. Hence, tolerance of anaerobic conditions during germination is an essential trait for direct-seeded rice cultivation in both rainfed and irrigated ecosystems. A QTL study was conducted to unravel the genetic basis of tolerance of anaerobic conditions during germination using a population derived from a cross between IR42, a susceptible variety, and Ma-Zhan Red, a tolerant landrace from China. Phenotypic data was collected based on the survival rates of the seedlings at 21 days after sowing of dry seeds under 10 cm of water. QTL analysis of the mapping population consisting of 175 F2:3 families genotyped with 118 SSR markers identified six significant QTLs on chromosomes 2, 5, 6, and 7, and in all cases the tolerant alleles were contributed by Ma-Zhan Red. The largest QTL on chromosome 7, having a LOD score of 14.5 and an R 2 of 31.7 %, was confirmed using a BC2F3 population. The QTLs detected in this study provide promising targets for further genetic characterization and for use in marker-assisted selection to rapidly develop varieties with improved tolerance to anaerobic condition during germination. Ultimately, this trait can be combined with other abiotic stress tolerance QTLs to provide resilient varieties for direct-seeded systems. 相似文献
2.
Yuling Li Yongbin Dong Suzhenniu Niu Dongqun Cui Yanzhao Wang Yanyang Liu Mengguan Wei Xuehui Li 《Molecular breeding : new strategies in plant improvement》2008,21(1):1-14
Specific traits are an important consideration in plant breeding. In popcorn, inferior agronomic traits could be improved
using dent or flint corn backcrossed with popcorn. In this study, we used advanced backcross quantitative trait locus (AB-QTL)
analysis to identify trait-improving QTL alleles from a dent maize inbred Dan232, and compared the detection of QTL in the
BC2S1 population with QTL results using F2:3 families of the same population. Two hundred and twenty BC2S1 families developed from a cross between Dan232 and an elite popcorn inbred N04 were evaluated for nine plant traits in replicated
field trials under two environments. Using composite interval mapping (CIM), a total of 28 significant QTL were detected,
and of these, 23 (82.14%) had favorable alleles contributed by the dent corn parent Dan232. Nine QTL (32.14%) detected in
the BC2S1 population were also located in or near the same chromosome intervals in the F2:3 population. All of the favorable QTL alleles from Dan232 could be used in marker-assisted selection (MAS) to improve the
respective plant traits in popcorn breeding. In addition, their near isogenic lines (QTL-NILs) could be obtained through selfing
or another 1–2 backcross with N04. Also, N04 improved for the studied plant traits could be developed from the BC2S1 families used in this study. This study demonstrated that the AB-QTL method can be applied to identify favorable QTL from
dent corn inbred in popcorn breeding and, once identified, the alleles could be used in marker-assisted selection to improve
the respective plant traits. 相似文献
3.
Identification and fine-mapping of a major QTL conferring resistance against head smut in maize 总被引:4,自引:0,他引:4
Chen Y Chao Q Tan G Zhao J Zhang M Ji Q Xu M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(8):1241-1252
Head smut is one of the most devastating diseases in maize, causing severe yield loss worldwide. Here we report identification
and fine-mapping of a major quantitative trait locus (QTL) conferring resistance to head smut. Two inbred lines ‘Ji1037’ (donor
parent, highly resistant) and ‘Huangzao4’ (recurrent parent, highly susceptible) were crossed and then backcrossed to ‘Huangzao4’
to generate BC populations. Four putative resistance QTLs were detected in the BC1 population, in which the major one, designated as qHSR1, was mapped on bin 2.09. The anchored ESTs, IDPs, RGAs, BAC and BAC-end
sequences in bin 2.09 were exploited to develop markers to saturate the qHSR1 region. The recombinants in the qHSR1 region
were obtained by screening the BC2 population and then backcrossed again to ‘Huangzao4’ to produce 59 BC2:3 families or selfed to generate nine BC2F2 families. Individuals from each BC2:3 or BC2F2 family were evaluated for their resistances to head smut and genotypes at qHSR1. Analysis of genotypes between the resistant
and susceptible groups within the same family allows deduction of phenotype of its parental BC2 recombinant. Based on the 68 BC2 recombinants, the major resistance QTL, qHSR1, was delimited into an interval of ~2 Mb, flanked by the newly developed markers
SSR148152 and STS661. A large-scale survey of BC2:3 and BC2F2 progeny indicated that qHSR1 could exert its genetic effect by reducing the disease incidence by ~25%.
Yongsheng Chen, Qing Chao and Guoqing Tan contributed equally to this work. 相似文献
4.
Wang P Zhou G Yu H Yu S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(8):1319-1330
Leaf size is a major determinant of plant architecture and yield potential in crops. A previous study showed that the genomic
region of chromosome 1 contains a major quantitative trait locus (QTL) for flag leaf size in a set of backcross recombinant
inbred lines derived from two elite parental lines (Zhenshan 97 and 93-11). In the present study, the QTL (qFL1) was shown to explain a large proportion of the variation in flag leaf size (leaf length, width and area) in derived populations
(BC2F3 and BC3F2) in multiple environments. Using a large segregating population, we narrowed the location of qFL1 to a 31 kb region containing four predicted genes. Expression of one of these genes, OsFTL1, differed between leaves in near-isogenic lines carrying alleles of Zhenshan 97 and 93-11. qFL1 had a pleiotropic effect on flag leaf size and yield-related traits. Conditional QTL analysis of the derived population (BC3F2) supports the assertion that qFL1 is the QTL for flag leaf length and exhibits pleiotropy. Pyramiding of qFL1 with two known genes (GS3 and Wx) from 93-11 into Zhenshan 97 enlarged flag leaves, improved grain size and amylose content, and increased yield per plant,
but slightly delayed heading date. These results provide a foundation for the functional characterization of the gene underlying
the pleiotropic effects of qFL1 and for genetic improvement of the plant architecture and yield potential of rice. 相似文献
5.
Li JZ Zhang ZW Li YL Wang QL Zhou YG 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(4):771-782
Grain yield is the most important and complex trait in maize. In this study, a total of 258 F9 recombinant inbred lines (RIL), derived from a cross between dent corn inbred Dan232 and popcorn inbred N04, were evaluated
for eight grain yield components under four environments. Quantitative trait loci (QTL) and their epistatic interactions were
detected for all traits under each environment and in combined analysis. Meta-analysis was used to integrate genetic maps
and detected QTL across three generations (RIL, F2:3 and BC2F2) derived from the same cross. In total, 103 QTL, 42 pairs of epistatic interactions and 16 meta-QTL (mQTL) were detected.
Twelve out of 13 QTL with contributions (R
2) over 15% were consistently detected in 3–4 environments (or in combined analysis) and integrated in mQTL. Only q100GW-7-1
was detected in all four environments and in combined analysis. 100qGW-1-1 had the largest R
2 (19.3–24.6%) in three environments and in combined analysis. In contrast, 35 QTL for 6 grain yield components were detected
in the BC2F2 and F2:3 generations, no common QTL across three generations were located in the same marker intervals. Only 100 grain weight (100GW)
QTL on chromosome 5 were located in adjacent marker intervals. Four common QTL were detected across the RIL and F2:3 generations, and two between the RIL and BC2F2 generations. Each of five important mQTL (mQTL7-1, mQTL10-2, mQTL4-1, mQTL5-1 and mQTL1-3) included 7–12 QTL associated with
2–6 traits. In conclusion, we found evidence of strong influence of genetic structure and environment on QTL detection, high
consistency of major QTL across environments and generations, and remarkable QTL co-location for grain yield components. Fine
mapping for five major QTL (q100GW-1-1, q100GW-7-1, qGWP-4-1, qERN-4-1 and qKR-4-1) and construction of single chromosome
segment lines for genetic regions of five mQTL merit further studies and could be put into use in marker-assisted breeding. 相似文献
6.
Dynamic QTL analysis of the Na+ content,K+ content,and Na+/K+ ratio in rice roots during the field growth under salt stress 总被引:1,自引:0,他引:1
J. Sun D. T. Zou F. S. Luan H. W. Zhao J. G. Wang H. L. Liu D. W. Xie D. Q. Su J. Ma Z. L. Liu 《Biologia Plantarum》2014,58(4):689-696
Rice (Oryza sativa L.) is seriously impacted by global soil salinization. To determine the quantitative trait loci (QTLs) related to salt tolerance in rice roots, F2:3 and BC1F2:3 populations derived from a cross between the cv. Dongnong 425 of high quality and yield and the salt-tolerant cv. Changbai 10, were studied at different development stages. Two genetic linkage maps of F2:3 and BC1F2:3 populations were constructed. A 66 mM NaCl solution was used to irrigate the field and to analyze the dynamic QTL of some rice root traits. Using unconditional and conditional QTL mapping methods, 30 unconditional QTLs and 16 conditional QTLs related to the 6 root traits were detected on the 9 rice chromosomes during different developmental stages. Fourteen pairs of unconditional and conditional QTLs were detected at the identical developmental stage in the identical population. A number of QTLs were detected at different developmental stages, however, many did not appear at the last stage. Remarkably, qRKC1 appeared continuously at multiple stages in both the populations suggesting its key role in regulating the salt tolerance of rice roots. 相似文献
7.
8.
Kohls S Stamp P Knaak C Messmer R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(2):327-338
Partial restoration of male fertility limits the use of C-type cytoplasmic male sterility (C-CMS) for the production of hybrid
seeds in maize. Nevertheless, the genetic basis of the trait is still unknown. Therefore, the aim to this study was to identify
genomic regions that govern partial restoration by means of a QTL analysis carried out in an F2 population (n = 180). This population was derived from the Corn Belt inbred lines B37C and K55. F2BC1 progenies were phenotyped at three locations in Switzerland. Male fertility was rated according to the quality and number
of anthers as well as the anthesis-silking interval. A weak effect of environment on the expression of partial restoration
was reflected by high heritabilities of all fertility-related traits. Partial restoration was inherited like an oligogenic
trait. Three major QTL regions were found consistently across environments in the chromosomal bins 2.09, 3.06 and 7.03. Therefore,
a marker-assisted counter-selection of partial restoration is promising. Minor QTL regions were found on chromosomes 3, 4,
5, 6 and 8. A combination of partial restorer alleles at different QTL can lead to full restoration of fertility. The maternal
parent was clearly involved in the partial restoration, because the restorer alleles at QTL in bins 2.09, 6.04 and 7.03 originated
from B37. The three major QTL regions collocated with other restorer genes of maize, a phenomenon, which seems to be typical
for restorer genes. Therefore, a study of the clusters of restorer genes in maize could lead to a better understanding of
their evolution and function. In this respect, the long arm of chromosome 2 is particularly interesting, because it harbors
restorer genes for the three major CMS systems (C, T and S) of maize. 相似文献
9.
D. D. Tuyen S. K. Lal D. H. Xu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(2):229-236
Salt-affected soils are generally classified into two main categories, sodic (alkaline) and saline. Our previous studies showed that the wild soybean accession JWS156-1 (Glycine soja) from the Kinki area of Japan was tolerant to NaCl salt, and the quantitative trait locus (QTL) for NaCl salt tolerance was located on soybean linkage group N (chromosome 3). Further investigation revealed that the wild soybean accession JWS156-1 also had a higher tolerance to alkaline salt stress. In the present study, an F6 recombinant inbred line mapping population (n = 112) and an F2 population (n = 149) derived from crosses between a cultivated soybean cultivar Jackson and JWS156-1 were used to identify QTL for alkaline salt tolerance in soybean. Evaluation of soybean alkaline salt tolerance was carried out based on salt tolerance rating (STR) and leaf chlorophyll content (SPAD value) after treatment with 180 mM NaHCO3 for about 3 weeks under greenhouse conditions. In both populations, a significant QTL for alkaline salt tolerance was detected on the molecular linkage group D2 (chromosome 17), which accounted for 50.2 and 13.0% of the total variation for STR in the F6 and the F2 populations, respectively. The wild soybean contributed to the tolerance allele in the progenies. Our results suggest that QTL for alkaline salt tolerance is different from the QTL for NaCl salt tolerance found previously in this wild soybean genotype. The DNA markers closely associated with the QTLs might be useful for marker-assisted selection to pyramid tolerance genes in soybean for both alkaline and saline stresses. 相似文献
10.
Marker-assisted selection for early-season cold tolerance in sorghum: QTL validation across populations and environments 总被引:1,自引:0,他引:1
Knoll J Ejeta G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(4):541-553
Sorghum [Sorghum bicolor (L.) Moench] landraces from China generally exhibit excellent emergence and seedling vigor under cool conditions, and are
being used as sources of genes for improvement of seedling cold tolerance in other cultivars. Marker-assisted selection (MAS)
could expedite the introgression of genes from landraces into elite lines, however, only a few studies have empirically demonstrated
efficacy of MAS for quantitatively inherited agronomic traits. In a preceding study we identified quantitative trait loci
(QTL) for early-season performance in a recombinant inbred (RI) population, one parent of which was a cold-tolerant Chinese
line, ‘Shan Qui Red’ (SQR). In this study, three SSR markers (Xtxp43, Xtxp51, and Xtxp211), each representing a QTL, were tested in two new populations: (Tx2794 × SQR F3) and (Wheatland × SQR BC1F3). Individual families were genotyped, and early-season field performance was measured for two years. Statistical analyses
showed that the SQR allele of Xtxp43 had favorable effects on seedling vigor in both populations, and on emergence in the Tx2794 population. A large positive
effect of the SQR allele of Xtxp51 was observed in the Tx2794 population for vigor and emergence. Slight genotype by environment interaction was observed for
Xtxp51 in the Wheatland population. Marker Xtxp211 had small but significant effects on seedling vigor and emergence in both populations. Various interactions between loci
were also significant. This study validated QTL markers in various genetic backgrounds, and demonstrated the utility of MAS
for a quantitative trait, early-season cold tolerance, evaluated in the field. 相似文献
11.
QTL mapping for plant-height traits has not been hitherto reported in high-oil maize. A high-oil maize inbred ‘GY220’ was
crossed with two dent maize inbreds (‘8984’ and ‘8622’) to generate two connected F2:3 populations. Four plant-height traits were evaluated in 284 and 265 F2:3 families. Single-trait QTL mapping and multiple-trait joint QTL mapping was used to detect QTLs for the traits and the genetic
relationship between plant height (PH) and two other plant-height traits. A total of 28 QTLs and 12 pairs of digenic interactions
among detected QTLs for four traits were detected in the two F2:3 families. Only one marker was shared between the two populations. Joint analysis of PH with ear height (EH) and PH with top
height (TH) detected 32 additional QTLs. Our results showed that QTL detection for PH was dependent on the genetic background
of dent corn inbreds. Multiple-trait joint QTL analysis could increase the number of detected QTLs. 相似文献
12.
13.
Huang XQ Kempf H Ganal MW Röder MS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(5):933-943
We report here the second advanced backcross quantitative trait locus (AB-QTL) analysis carried out in winter wheat. Seven agronomic traits were studied in a BC2F1population derived from a cross between the German winter wheat variety Flair and the synthetic wheat line XX86 developed in Japan. We selected 111 BC2F1 lines and genotyped these with 197 microsatellite markers. Field data for seven agronomic traits were collected from corresponding BC2F3 families that were grown at up to six locations in Germany. QTL analyses for yield and yield components were performed using single-marker regression and interval mapping. A total of 57 putative QTLs derived from XX86 were detected, of which 24 (42.1%) were found to have a positive effect from the synthetic wheat XX86. These favourable QTLs were mainly associated with thousand-grain weight and grain weight per ear. Many QTLs for correlated traits were mapped in similar chromosomal regions. The AB-QTL data obtained in the present study are discussed and compared with results from previous QTL analyses. 相似文献
14.
Mapping of QTL associated with chilling tolerance during reproductive growth in soybean 总被引:6,自引:0,他引:6
Funatsuki H Kawaguchi K Matsuba S Sato Y Ishimoto M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(5):851-861
Low temperatures in summer bring about drastic reduction in seed yield of soybean [Glycine max (L.) Merr.]. To identify quantitative trait loci (QTL) associated with chilling tolerance during the reproductive growth
in soybean, a recombinant inbred line (RIL) population consisting of 104 F6-derived lines was created from a cross between two cultivars, chilling-tolerant Hayahikari and chilling-sensitive Toyomusume.
The RIL were genotyped with 181 molecular and phenotypic markers and were scored with regard to chilling tolerance, which
was evaluated by comparison of seed-yielding abilities in two artificial climatic environments at chilling and usual temperatures.
Three QTL were detected for chilling tolerance in seed-yielding ability. Two of them, qCTTSW1 and qCTTSW2, were mapped near QTL for flowering time, and the latter had an epistatic interaction with a marker locus located near another
QTL for flowering time, where no significant QTL for chilling tolerance was detected. The analysis of an F2 population derived from the cross between Hayahikari and an RIL of the Hayahikari genotype at all QTL for flowering time
confirmed the effect of the third QTL, qCTTSW3, on chilling tolerance and suggested that qCTTSW1 was basically independent of the QTL for flowering time. The findings and QTL found in this study may provide useful information
for marker-assisted selection (MAS) and further genetic studies on soybean chilling tolerance. 相似文献
15.
J. W. Dudley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,85(1):101-104
The objective of this paper is to present genetic theory demonstrating the conditions under which it should be possible to identify molecular marker-quantitative trait locus (QTL) associations in crosses of random-mating populations to inbreds. Using as an example the cross of a corn (Zea mays L.) population to an inbred, the expected disequilibrium for testcross and per se performance of F2, F3, BC1 (to the inbred) and recombinant inbred generations was derived for cases where a marker allele is linked to an unfavorable QTL allele in the inbred and where the marker allele is linked to a favorable QTL allele in the inbred. Disequilibrium in segregating generations was shown to be a function of disequilibrium in the parent population, the frequency of marker and QTL alleles in the parent population, and the recombination distance between the marker and the QTL. To maximize the opportunity to identify a favorable QTL the following procedures are suggested:
Communicated by A. R. Hallauer 相似文献
(1) | Select marker loci with alleles in the parent population which are not present in the inbred. |
(2) | Select populations known to have favorable QTL alleles not present in the inbred. |
(3) | Use as many marker loci as possible to enhance the probability of tight linkage between the marker and the QTL. |
16.
Mengguan Wei Xuehui Li Junzhou Li Jiafeng Fu Yanzhao Wang Yuling Li 《Plant Physiology and Biochemistry》2009,47(10):886-894
Both yield and quality traits for stover portion were important for forage and biofuel production utility in maize. A high-oil maize inbred GY220 was crossed with two normal-oil dent maize inbred lines 8984 and 8622 to generate two connected F2:3 populations with 284 and 265 F2:3 families. Seven yield and quality traits were evaluated under two environments. The variance components of genotype (σg2), environment (σe2) and genotype × environment interactions (σge2) were all significant for most traits in both populations. Different levels of correlations were observed for all traits. QTL mapping was conducted using composite interval mapping (CIM) for data under each environment and in combined analysis in both populations. Totally, 45 and 42 QTL were detected in the two populations. Only five common QTL across the two populations, and one and three common QTL across the two environments in the two populations were detected, reflecting substantial influence of genetic backgrounds and environments on the results of QTL detection for stover traits. Combined analysis across two environments failed to detect most QTL mapped using individual environmental data in both populations. Few of the detected QTL displayed digenic epistatic interactions. Common QTL among all traits were consistent with their correlations. Some QTL herein have been detected in previous researches, and linked with candidate genes for enzymes postulated to have direct and indirect roles in cell wall components biosynthesis. 相似文献
17.
Micic Z Hahn V Bauer E Schön CC Knapp SJ Tang S Melchinger AE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(7):1474-1484
In many sunflower-growing regions of the world, Sclerotinia sclerotiorum (Lib.) de Bary is the major disease of sunflower (Helianthus annuus L.). In this study, we mapped and characterized quantitative trait loci (QTL) involved in resistance to S. sclerotiorum midstalk rot and two morphological traits. A total of 351 F3 families developed from a cross between a resistant inbred line from the germplasm pool NDBLOS and the susceptible line CM625 were assayed for their parental F2 genotype at 117 codominant simple sequence repeat markers. Disease resistance of the F3 families was screened under artificial infection in field experiments across two sowing times in 1999. For the three resistance traits (leaf lesion, stem lesion, and speed of fungal growth) and the two morphological traits, genotypic variances were highly significant. Heritabilities were moderate to high (h2=0.55–0.89). Genotypic correlations between resistance traits were highly significant (P<0.01) but moderate. QTL were detected for all three resistance traits, but estimated effects at most QTL were small. Simultaneously, they explained between 24.4% and 33.7% of the genotypic variance for resistance against S. sclerotiorum. Five of the 15 genomic regions carrying a QTL for either of the three resistance traits also carried a QTL for one of the two morphological traits. The prospects of marker-assisted selection (MAS) for resistance to S. sclerotiorum are limited due to the complex genetic architecture of the trait. MAS can be superior to classical phenotypic selection only with low marker costs and fast selection cycles. 相似文献
18.
19.
Lei Zhou Yawen Zeng Weiwei Zheng Bo Tang Shuming Yang Hongliang Zhang Jinjie Li Zichao Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(5):895-905
Low temperature at the booting stage is a serious abiotic stress in rice, and cold tolerance is a complex trait controlled
by many quantitative trait loci (QTL). A QTL for cold tolerance at the booting stage in cold-tolerant near-isogenic rice line
ZL1929-4 was analyzed. A total of 647 simple sequence repeat (SSR) markers distributed across 12 chromosomes were used to
survey for polymorphisms between ZL1929-4 and the cold-sensitive japonica cultivar Towada, and nine were polymorphic. Single
marker analysis revealed that markers on chromosome 7 were associated with cold tolerance. By interval mapping using an F2 population from ZL1929-4 × Towada, a QTL for cold tolerance was detected on the long arm of chromosome 7. The QTL explained
9 and 21% of the phenotypic variances in the F2 and F3 generations, respectively. Recombinant plants were screened for two flanking markers, RM182 and RM1132, in an F2 population with 2,810 plants. Two-step substitution mapping suggested that the QTL was located in a 92-kb interval between
markers RI02905 and RM21862. This interval was present in BAC clone AP003804. We designated the QTL as qCTB7 (quantitative trait locus for cold tolerance at the booting stage on chromosome 7), and identified 12 putative candidate
genes. 相似文献
20.
Ratnam Wickneswari M. A. R. Bhuiyan Sabu Kalluvettankuzhy K. Li Sze Lim Michael J. Thomson Md. Kairudin Narimah Md. Zain Abdullah 《Plant Molecular Biology Reporter》2012,30(4):929-939
A backcross breeding strategy was used to identify quantitative trait loci (QTLs) associated with 14 traits in a BC2F2 population derived from a cross between MR219, an indica rice cultivar and an accession of Oryza rufipogon (IRGC 105491). A total of 261 lines were genotyped with 96 microsatellite markers and evaluated for plant morphology, yield components and growth period. The genetic linkage map generated for this population with an average interval size of 16.2?cM, spanning 1,553.4?cM (Kosambi) of the rice genome. Thirty-eight QTLs were identified with composite interval mapping (CIM), whereas simple interval mapping (SIM) resulted in 47 QTLs (LOD >3.0). The O. rufipogon allele was favourable for 59% of QTLs detected through CIM. Of 261 BC2F2 families, 26 advanced backcross breeding lines (BC2F5) were used for QTL validation. These lines were selected on the basis of the yield traits potentiality in BC2F3 and BC2F4 generations. The field trial was conducted at three different locations in Malaysia using randomized complete block design with three replications. Trait based marker analysis was done for QTL determination. Twenty-five QTLs were detected in BC2F5 generation whereas 29 QTLs were detected in BC2F2 generation of the same population. Two QTLs (qPL-1 and qSPL-7) were not considered for validation due to their low R 2 values and two QTLs (qPSS-3-2 and qGW-3-2) were not detected in the BC2F5 population. Fifteen QTLs showed the beneficial effect to enhance the trait value of the breeding lines. QTL validation aided to select the promising lines for further utilization. 相似文献