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1.
Vanadium Requirements and Uptake Kinetics in the Dinitrogen-Fixing Bacterium Azotobacter vinelandii
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Vanadium is a cofactor in the alternative V-nitrogenase that is expressed by some N2-fixing bacteria when Mo is not available. We investigated the V requirements, the kinetics of V uptake, and the production of catechol compounds across a range of concentrations of vanadium in diazotrophic cultures of the soil bacterium Azotobacter vinelandii. In strain CA11.70, a mutant that expresses only the V-nitrogenase, V concentrations in the medium between 10−8 and 10−6 M sustain maximum growth rates; they are limiting below this range and toxic above. A. vinelandii excretes in its growth medium micromolar concentrations of the catechol siderophores azotochelin and protochelin, which bind the vanadate oxoanion. The production of catechols increases when V concentrations become toxic. Short-term uptake experiments with the radioactive isotope 49V show that bacteria take up the V-catechol complexes through a regulated transport system(s), which shuts down at high V concentrations. The modulation of the excretion of catechols and of the uptake of the V-catechol complexes allows A. vinelandii to precisely manage its V homeostasis over a range of V concentrations, from limiting to toxic. 相似文献
2.
Trautsch J Rosseland BO Pedersen SA Kristiansen E Zachariassen KE 《Journal of insect physiology》2011,57(8):1123-1126
As the body fluid of freeze-tolerant organisms freezes, solutes become concentrated in the gradually smaller unfrozen fluid fraction, and dissolved trace metals may reach toxic levels. A dialysis technique was used to investigate the metal binding capacity of the low density fraction of the hemolymph from the freeze tolerant beetle Phyto depressus. The low density fraction, assumed to contain the ice nucleating lipoproteins, showed approximately 100 times greater capacity to bind metals (Cd 2+, Cu 2+ and Zn 2+) than the proteins albumin, hemoglobin and similar to metallothionein. The high metal binding capacity in the low density fraction raises the question if the ice nucleating lipoproteins might assist in detoxification of potentially toxic concentrations of metals that may occur when a large fraction of the bodyfluids of freeze tolerant insects freeze. This hypotheis is consistent with the fact that the lipoprotein ice nucleators are present in far greater amounts than required for ice nucleation, and also with the fact that the lipoprotein ice nucleators have a remarkably high content of amino acids with negatively charged residues that may act as metal binding sites. 相似文献
3.
Increased persistence in Escherichia coli caused by controlled expression of toxins or other unrelated proteins 总被引:1,自引:0,他引:1
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Bacterial populations contain persisters, cells which survive exposure to bactericidal antibiotics and other lethal factors. Persisters do not have a genetic resistance mechanism, and their means to tolerate killing remain unknown. In exponentially growing populations of Escherichia coli the frequency of persister formation usually is 10−7 to 10−5. It has been shown that cells overexpressing either of the toxic proteins HipA and RelE, both members of the bacterial toxin-antitoxin (TA) modules, have the ability to form more persisters, suggesting a specific role for these toxins in the mechanism of persistence. However, here we show that cells expressing proteins that are unrelated to TA modules but which become toxic when ectopically expressed, chaperone DnaJ and protein PmrC of Salmonella enterica, also form 100- to 1,000-fold more persisters. Thus, persistence is linked not only to toxicity caused by expression of HipA or dedicated toxins but also to expression of other unrelated proteins. 相似文献
4.
Ting-Ting Zhang Chun-Yan Zheng Wei Hu Wen-Wu Xu Hao-Fen Wang 《Journal of applied phycology》2010,22(1):71-77
Cyanobacterial contamination of water has been a serious problem in recent years. Thus, the effective control of undesired
cyanobacteria has become an urgent issue. We studied therefore the effects of ρ-coumaric acid and vanillic acid on toxic Microcystis aeruginosa and the allelopathic mechanisms. The results showed that the growth of toxic M. aeruginosa was significantly inhibited by ρ-coumaric acid and vanillic acid, with an EC50 of 0.26 ± 0.07 and 0.34 ± 0.05 mmol L−1, respectively. Our data also demonstrated that both ρ-coumaric acid and vanillic acid triggered the generation of superoxide
anion radicals (O2
•−). The O2
•− might induce a lipid peroxidation which may change cell membrane penetrability, thereby leading to the eventual death of
M. aeruginosa. Our current studies further provide evidence that some phenolic acids such as ρ-coumaric acid and vanillic acid may be a
potential effective solution for aquatic management. 相似文献
5.
Production of extracellular polymeric substances from Rhodopseudomonas acidophila in the presence of toxic substances 总被引:2,自引:0,他引:2
A hydrogen-producing photosynthetic bacteria strain, Rhodopseudomonas acidophila, was used to investigate the production of extracellular polymeric substances (EPS) in the presence of toxic substances and the effect of toxicants on bacterial surface characteristics. Addition of the toxic substances including Cu(II), Cr(VI), Cd(II) and 2,4-dichlorophenol (2,4-DCP) stimulated the production of EPS but reduced the cell dry weight. At concentrations of 30 mg l−1 Cu(II), 40 mg l−1 Cr(VI), 5 mg l−1 Cd(II) and 100 mg l−1 2,4-DCP, the EPS content increased by 5.5, 2.5, 4.0 and 1.4 times, respectively, than the control. These toxic substances also greatly influenced the proteins/carbohydrates ratio of EPS. The ratios in the presence of toxic substances were always higher than that of control. Furthermore, under toxic conditions, the increase in the protein content far exceeded than that of others in EPS, suggesting that extracellular proteins could protect cells against toxic substances. The toxic substances significantly changed the surface characteristics and flocculation ability of R. acidophila, such as surface energy, relative hydrophobicity and free energy of adhesion. 相似文献
6.
Abnormal myo-[2-3H]inositol incorporation into phosphatidylinositol has been found in phentolamine-treated synaptosomes that were isolated from the cerebral hemispheres of galactose toxic rats and incubated with [33P]Pi and myo-[2-3H] inositol. In galactose toxic rats phentolamine-stimulated myo-[2-3H]inositol labeling of phosphatidylinositol was 70% greater than in normal animals. This enhanced labeling of synaptosomal phosphatidylinositol in galactose toxic rats during stimulation with phentolamine is in marked contrast to the depressed myo-inositol labeling of phosphatidylinositol reported with acetylcholine stimulation. 相似文献
7.
Brown trout were exposed for 63 days to five treatments: a control; the purified cyanobacterial hepatotoxin microcystin—LR (MC—LR) (41—57 μg MC—LR 1?1); lysed toxic Microcystis aeruginosa cells (41–68 μg MC—LR 1?1 and 288 μg chlorophyll a 1?1); lysed non—toxic M. aeruginosa cells (non—MC—LR containing and 288 μg chlorophyll a 1?1); ammonia (65–325 μg NH3 1?1). All treatments produced significantly reduced growth compared to controls (P<0·05, Fisher test). Exposure to ammonia resulted weight loss over the first 7 days followed by weight increase, though at a significantly lower level than in the other treatments. First exposed to lysed toxic M. aeruginosa cells grew less than those exposed to lysed non—toxic cyanobacteria or purified MC—LR. Sodium influx rates after 63 days exposure to purified MC—LR, lysed toxic M. aeruginosa cells, or ammonia showed a significant increase compared to control fish or those exposed to lysed non—toxic M. aeruginosa cells. There were no significant differences in Na+ efflux or net Na+ uptake rates between treatments. Significant increases in body Na+ and Cl— were seen in fish exposed to lysed toxic M. aeruginosa cells or ammonia. Only fish exposed to ammonia showed a significant increase in body ammonia. Short—term exposure, over 4 h, to lysed toxic cells, non—toxic cells or purified MC—LR resulted in insignificant changes in Na+ flux rates compared to controls although there was a significant net Na+ loss in fish exposed to ammonia. Chronic exposure of fish to toxic cyanobacterial blooms may result in ionic imbalance and reduced growth. 相似文献
8.
《Harmful algae》2014
Occurrence of toxic cyanobacterial blooms has become a worldwide problem, increasing the risk of human poisoning due to consumption of seafood contaminated with cyanotoxins. Though no such cases of human intoxication due to toxic blooms have been reported so far from India, most of the studies related to blooms have been restricted to reporting of a bloom and/or antimicrobial activity of its extract. Detailed toxicity study of cyanobacterial blooms are lacking. A study on the toxicity of a dense bloom (14.56 × 106 trichomes L−1) of the marine diazotrophic cyanobacteria, Trichodesmium erythraeum, observed in the coastal waters of Phoenix Bay, Port Blair, Andamans was undertaken. The significance of this bloom is that it was a single species and had conspicuously inhibited the growth of other phytoplankton and complete exclusion of zooplankton from the bloom region, intimating the involvement of toxins in the bloom. The cyanobacterial extracts showed prominent antimicrobial activity against certain human pathogenic bacteria and fungi. Studies on the toxicity of the cyanobacterial extracts was carried out using brine shrimp bioassay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and comet assay. The cyanobacterial extract exhibited toxic effect to Artemia salina causing mortality of up to 40% after 48 h at a concentration of 1 mg mL−1, while it induced cytotoxicity in cell lines (HepG2 and HaCat) and caused DNA damage in human lymphocytes in vitro. 相似文献
9.
Effect of zinc binding on β-amyloid structure and dynamics: implications for Aβ aggregation 总被引:1,自引:0,他引:1
Assembly of β-amyloid (Aβ) peptide into toxic oligomers is widely believed to initiate Alzheimer's disease pathogenesis. Under in vitro physiological conditions, zinc (Zn(II)) can bind to Aβ and redirect its assembly from amyloid fibrillar toward less toxic amorphous aggregation. Propensity of Aβ to go toward a specific form of aggregate state is determined by structural and dynamical properties of the initial monomeric as well as the aggregate state. Here we probe the structural and dynamical impact of binding of Zn(II) to monomeric Aβ40 using NMR spectroscopy. To obtain further support for the importance of intrinsic dynamics in the aggregation precursor, 15N relaxation measurements were also performed for Aβ42, the more fibrillar aggregation-prone variant of Aβ. The combined data suggest that, upon Zn(II)-binding to the N-terminus of Aβ40, a relatively rigid turnlike structure is induced at residues Val24-Lys28 whereas the residues flanking this region become more mobile on the picosecond-to-nanosecond timescale. This is in contrast to the increased rigidity of Aβ42 at the C-terminus, and proposed to be linked to the higher propensity of Zn(II)-bound peptide to form amorphous aggregates with less entropic penalties than their fibrillar counterparts. 相似文献
10.
Jean Ballongue Eberhard Masion Jamal Amine Henri Petitdemange Robert Gay 《Applied microbiology and biotechnology》1987,26(6):568-573
Summary The growth of Clostridium acetobutylicum was studied by three ways. 1. In batch fermentation, referred to as the control. 2. Fermentation in dialysis which permits
elimination of all the products of metabolism: acids, solvents and gases. In order to test the toxic effect of acids, cultures
were dialysed against 2 g l-1 acetic acid or 2 g l-1 butyric acid. 3. To test the toxic effect of gases only, batch fermentations were carried out under vacuum or with a continuous
bubbling of nitrogen.
The first method resulted in a productivity of 1.2 g l-1 dry cell weight and a maximal specific growth rate of 0.2 h-1; the second, 20 g l-1 dry cell weight and a constant maximum specific growth rate (μ=0.39 h-1) between 14 and 20 h. The toxic effect of acetic and butyric acids, starts at low concentrations and about 4 g l-1 of both acids results in a decrease of 50% of maximal specific growth rate. The third series of experiments showed that gases
produced by the bacteria have a high toxic effect, comparable to that of 5 g l-1 of acid. 相似文献
11.
Summary The growth ofPseudomonas tabaci in nutrient medium is partially inhibited in the presence of 10–3 M added nickel (threshold toxic concentration), with complete inhibition at 10–2 M nickel—but no effect at 10–4 and 10–5 M. Toxic levels of nickel affect both cell division and cell viability.Spectrophotometric determination of intracellular levels of nickel at different external concentrations showed that the highest internal values occurred with cells cultured in 10–4M (non-toxic) nickel medium rather than in 10–3 (toxic) medium—suggesting that nickel toxicity does not primarily relate to internal concentration.X-ray microanalysis, carried out on whole bacterial cells, showed that toxic levels of nickel in the external medium resulted in a range of ionic changes in the cell, including a decrease in the level of K (K efflux) and an increase in the levels of Mn, Fe, Ni, and Cu (transition metal cation influx). Other changes induced by nickel toxicity included an increase in the level of soluble S (with a decrease in insoluble S), an increased cell dry mass, and a conspicuous plasmolysis—which was observed both in whole cells and in ultrathin sections.The results obtained support a primary toxic effect of nickel at the cell surface—possibly directly affecting the transport activity of the plasmalemma. The resulting changes, particularly involving the influx of a range of cations, may lead to secondary toxic activities affecting the whole metabolism, leading to plasmolysis and inhibition of division. 相似文献
12.
S. V. Isayenkov 《Cytology and Genetics》2012,46(5):302-318
The study of salt stress mechanisms in plants has become an important issue for the modern agricultural development, climate change, and global food crisis. The plant response to high salt concentrations is complex and comprehensive; it includes many different processes, which should be correctly coordinated. The effect of excessive salt concentrations on plants results in osmotic stress and creates an ionic inbalance due to the accumulation of toxic ions, such as Cl? and, especially, Na+. Salt stress also has negative impact on mineral homeostasis, in particular Ca2+ and K+. The progress in transcryptomics, genomics, and molecular biology revealed a new gene families that participate in the formation of salt stress response in plants. This review describes the fundamental principles and mechanisms of plant salt tolerance, maintenance of ion homeostasis. In this paper the detailed analysis of the maine transport membrane systems responsible for the transport of ions and their role in plant salt stress were conducted. The perspectives and directions for the further biotechnological and genetic improvement of salt tolerance in plants are underlied. 相似文献
13.
D A Rigas C Eginitis-Rigas C Head 《Biochemical and biophysical research communications》1979,88(2):373-379
A new type of toxicity biphasically dependent on concentration was observed with diethyldithiocarbamate, a metal chelator utilized in medicine. As judged by cell survival and [3H]Urd incorporation, diethyldithiocarbamate was maximally toxic to T lymphocytes and polymorphonuclears at 2.5×10?5 M (first phase) and at higher than 2.5×10?3 M (second phase), but was not toxic at intermediate concentrations around 2.5×10?4 M. The response of chelator treated T lymphocytes to phytohemagglutinin was also biphasic. The first toxic phase was partially reversed by 2.5×10?5 M ZnCl2, while the second phase was partially reversed by 10?2 M CuCl2. This suggests that inhibition of Zn-metalloenzymes in the first phase and of Cu-metalloenzymes in the second may play a crucial role in the mechanism of toxicity. The second toxic phase may be in part due to the observed inhibition of superoxide dismutase rendering the cells susceptible to oxygen toxicity, like obligate anaerobes. 相似文献
14.
A. Shiryaeva A. Arkadyeva L. Emelyanova G. Sakuta V. Morozov 《Journal of bioenergetics and biomembranes》2009,41(4):379-385
The progression of toxic hepatitis is accompanied by the activation of oxidative processes in the liver associated with an
enhancement of the mitochondrial respiratory chain activity and superoxide anion production (О2˙-). The purpose of this study was to examine our previously formulated assumption concerning the predominant contribution of
the complex I to О2˙- production increase by the mitochondrial respiratory chain of hepatocytes in toxic hepatitis (Shiryaeva et al. Tsitologiia,
49, 125–132 2007). Toxic hepatitis was induced by a combined application of ССl4 and ethanol. Respiratory chain function analysis was executed with submitochondrial particles (SP) in the presence of specific
inhibitors. It was shown that the rate of О2˙- production by SP of animals with toxic hepatitis, when NADH was delivered, was 2.5-fold higher as compared with the control.
The rates of О2˙- production by SP of rats with toxic hepatitis in the presence of NADH or NADH + rotenone were similar. The О2˙- production rate by control SP in the presence of NADH + rotenone corresponded to the О2˙- production rate by toxic hepatitis SP when only NADH was delivered. When NADH + myxothiazol were delivered to the incubation
system, О2˙- production by toxic hepatitis SP was 72% higher than for the control. Conversely, in the presence of antimycin A, the production
of О2˙- by toxic hepatitis SP was lower compared to the control. Collectively, the presented data indicate that the О2˙- production rate was enhanced by the complex I of the hepatocyte mitochondrial respiratory chain in experimental toxic hepatitis.
Complex III contribution to the production of О2˙- was insignificant. We assume that the increase in О2˙- production by the respiratory chain may be considered not only as the mechanism of pathology progression, but also as a compensatory
mechanism preserving the electron transport function of the mitochondrial respiratory chain when complex I functioning is
blocked in part. 相似文献
15.
This paper studies the species composition and quantitative distribution of diatoms that belong to the genus Pseudo-nitzschia in the Russian waters of the Sea of Japan and the Sea of Okhotsk. In total, 11 species of this genus were found in the area, including 7 that are known as being potentially toxic. The highest concentrations of Pseudo-nitzschia microalgae (1.4 × 106–2.7 × 106 cells/L) were found in the summer and autumn in the Peter the Great Bay of the Sea of Japan and the lowest concentrations (2.5 × 102–1 × 104 cells/L) were found in the Sakhalinsky and Akademiya bays of the Sea of Okhotsk. The species diversity of potentially toxic diatoms was greatest (seven species) and the cell concentrations highest (over 6 × 105 cells/L) in the Peter the Great Bay, Sea of Japan, and in the Aniva Bay, Sea of Okhotsk. The density of potentially toxic species was highest near the northeastern coast of Sakhalin Island, in the Amur River estuary, and in adjacent waters. This paper also presents geographical distribution maps of Pseudo-nitzschia species and maps of the density distribution of potentially toxic microalgae over the studied area and identifies potential amnesic shellfish poisoning areas. 相似文献
16.
Ulrike K. Resenberger Veronika Müller Lisa M. Munter Michael Baier Gerd Multhaup Mark R. Wilson Konstanze F. Winklhofer J?rg Tatzelt 《The Journal of biological chemistry》2012,287(52):43765-43776
The heat shock response (HSR) is an evolutionarily conserved pathway designed to maintain proteostasis and to ameliorate toxic effects of aberrant protein folding. We have studied the modulation of the HSR by the scrapie prion protein (PrPSc) and amyloid β peptide (Aβ) and investigated whether an activated HSR or the ectopic expression of individual chaperones can interfere with PrPSc- or Aβ-induced toxicity. First, we observed different effects on the HSR under acute or chronic exposure of cells to PrPSc or Aβ. In chronically exposed cells the threshold to mount a stress response was significantly increased, evidenced by a decreased expression of Hsp72 after stress, whereas an acute exposure lowered the threshold for stress-induced expression of Hsp72. Next, we employed models of PrPSc- and Aβ-induced toxicity to demonstrate that the induction of the HSR ameliorates the toxic effects of both PrPSc and Aβ. Similarly, the ectopic expression of cytosolic Hsp72 or the extracellular chaperone clusterin protected against PrPSc- or Aβ-induced toxicity. However, toxic signaling induced by a pathogenic PrP mutant located at the plasma membrane was prevented by an activated HSR or Hsp72 but not by clusterin, indicating a distinct mode of action of this extracellular chaperone. Our study supports the notion that different pathological protein conformers mediate toxic effects via similar cellular pathways and emphasizes the possibility to exploit the heat shock response therapeutically. 相似文献
17.
Ricin has been coupled to the cleavable, photolabile, hetero-bifunctional cross-linking reagent N-[4-(p-azidophenylazo)benzoyl] 3-aminopropyl-N1-oxysuccinimide ester. Approx. 1.3–1.9 moles/mole of ricin are present in the conjugate. The conjugate is fully toxic when tested in the dark on intact cells. Cells, incubated with low amounts of the conjugate at 2 °C for 45 min and shifted to 37 °C for 3 h, become markedly protected against the effect of low concentrations of the toxin if irradiated after 2.5–3 h, when the toxic effect on protein synthesis in non-irradiated cells is maximal. Irradiation at intermediate times produces no or only partial protection. Less protection is obtained with high concentrations of the conjugate or in the presence of methylamine, a reagent that enhances uptake of ricin into cells.Subcellular fractionation of cells exposed in the dark to the photolabile ricin derivative for various times reveals that the conjugate is cross-linked to plasma membrane-derived fractions at early times. After 45 min at 37 °C the ricin is associated with subcellular fractions enriched in lysosomes and Golgi-derived elements and after prolonged times at 37 °C with subcellular fractions supporting RNA synthesis. 相似文献
18.
The production of toxins by Botrytis fabae in relation to growth of lesions on field bean leaves at different humidities 总被引:2,自引:0,他引:2
J. G. HARRISON 《The Annals of applied biology》1980,95(1):63-71
Filtrate extracts from liquid cultures of B. fabae and extracts from spreading chocolate spot lesions contained at least two heat-stable, light-labile phytotoxic compounds. Lesions similar in appearance to those of chocolate spot developed after injection of fractions containing these compounds into healthy bean leaves. Of 15 plant species injected with an extract from lesions, Vicia faba appeared to be the most susceptible to damage. Evidence is presented to support the hypothesis that under conditions of low humidity the concentrations of toxic compounds in an infected leaf become high enough to kill healthy cells surrounding infected tissue. The dead tissue then dries out preventing further fungal growth and lesion spread. In saturated air, however, the toxic compounds diffuse throughout the lamina and become too dilute to kill uninfected tissue. Tissue does not become desiccated and the fungus continues to spread. 相似文献
19.
The soluble tellurium oxyanion, tellurite, is toxic for most organisms. At least in part, tellurite toxicity involves the
generation of oxygen-reactive species which induce an oxidative stress status that damages different macromolecules with DNA,
lipids and proteins as oxidation targets. The objective of this work was to determine the effects of tellurite exposure upon
the Escherichia coli pyruvate dehydrogenase (PDH) complex. The complex displays two distinct enzymatic activities: pyruvate dehydrogenase that
oxidatively decarboxylates pyruvate to acetylCoA and tellurite reductase, which reduces tellurite (Te4+) to elemental tellurium (Teo). PDH complex components (AceE, AceF and Lpd) become oxidized upon tellurite exposure as a consequence of increased carbonyl
group formation. When the individual enzymatic activities from each component were analyzed, AceE and Lpd did not show significant
changes after tellurite treatment. AceF activity (dihydrolipoil acetyltransferase) decreased ~30% when cells were exposed
to the toxicant. Finally, pyruvate dehydrogenase activity decreased >80%, while no evident changes were observed in complex′s
tellurite reductase activity. 相似文献
20.
Lithium-induced Hypersensitivity to Foot Shock in Rats and the Role of 5-Hydroxytryptophan 总被引:1,自引:0,他引:1
REPORTS of the behavioural effects of lithium salts on animals mainly seem to have dealt with depressant effects on spontaneous activities or with toxic symptoms (weight loss, polyuria, polydipsia, diarrhoea and so on). After prolonged lithium treatment, changes in brain 5-hydroxytryptamine (5HT) metabolism have been found to occur; 5HT turnover is decreased either in the whole brain1 or in specific areas such as brainstem and hypothalamus1, 2, where the levels are also decreased2. When levels of 5HT are reduced in the whole brain of rats either by lesions3 or by parachlorophenylalanine (PCPA)4, an inhibitor of 5HT synthesis, motor responsiveness of rats to electrical stimulation of the feet has been found to increase. We have observed that rats treated with lithium for a few days struggle more than controls when the skin is punctured in the course of injections and after 2 weeks of treatment with lithium chloride (LiCl), foot shock “jump response” thresholds are reduced by about 10 and 25% with doses of 1 and 2 mequiv./kg respectively. With larger doses, sensitivity to foot shock is not increased further, but may even decline as toxic effects appear; after 2 weeks of administration of 3 mequiv/kg LiCl, toxic effects appeared in nearly all our rats and about 10% of animals died. Sheard5 has found that treatment for 5 days with a high dose of LiCl (5 mequiv/kg) had no effect on motor responsiveness to foot shock, although shock-induced aggressive behaviour decreased; no toxic effects were reported. 相似文献