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1.
R. Kölliker E. S. Jones M. C. Drayton M. P. Dupal J. W. Forster 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):416-424
Highly informative molecular markers, such as simple sequence repeats (SSRs), can greatly accelerate breeding programs. The
aim of this study was to develop and characterise a comprehensive set of SSR markers for white clover (Trifolium repens L.), which can be used to tag genes and quantitative trait loci controlling traits of agronomic interest. Sequence analysis
of 1123 clones from genomic libraries enriched for (CA)
n
repeats yielded 793 clones containing SSR loci. The majority of SSRs consisted of perfect dinucleotide repeats, only 7% being
trinucleotide repeats. After exclusion of redundant sequences and SSR loci with less than 25 bp of flanking sequence, 397
potentially useful SSRs remained. Primer pairs were designed for 117 SSR loci and PCR products in the expected size range
were amplified from 101 loci. These markers were highly polymorphic, 88% detecting polymorphism across seven white clover
genotypes with an average allele number of 4.8. Four primer pairs were tested in an F2 population revealing Mendelian segregation. Successful cross-species amplification was achieved in at least one out of eight
legume species for 46 of 54 primer pairs. The rate of successful amplification was significantly higher for Trifolium species when compared to species of other genera. The markers developed in this study not only provide valuable tools for
molecular breeding of white clover but may also have applications in related taxa.
Received: 3 April 2000 / Accepted: 12 May 2000 相似文献
2.
Analysis of SSRs derived from grape ESTs 总被引:72,自引:0,他引:72
K. D. Scott P. Eggler G. Seaton M. Rossetto E. M. Ablett L. S. Lee R. J. Henry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(5):723-726
One hundred and twenty four microsatellites were isolated from analysis of 5000 Vitis expressed sequence tags (ESTs). A diversity of dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were present.
Primers were designed for 16 of these SSRs and they were tested on seven accessions. Ten of the sixteen primer pairs resulted
in PCR products of the expected size. All ten functional primers were polymorphic across the accessions studied. Polymorphisms
were evident at the level of cultivars, Vitis species, and between related genera. SSRs that were from the 3′ untranslated region (3′UTR) were most polymorphic at the cultivar
level, the 5′ untranslated region (5′ UTR) SSRs were most polymorphic between cultivars and species, and those SSRs within
coding sequence were most polymorphic between species and genera. These results show that EST-derived SSRs in Vitis are useful as they are polymorphic and highly transferable. With EST SSRs being applicable to studies at several taxonomic
levels, the large number of SSRs (approximately 1000) that will be available from an expanded EST database of 45 000 will
have many potential applications in mapping and identity research.
Received: 4 June 1999 / Accepted: 21 September 1999 相似文献
3.
J.-M. Lacape D. Dessauw M. Rajab J.-L. Noyer B. Hau 《Molecular breeding : new strategies in plant improvement》2007,19(1):45-58
A series of 320 mapped simple sequence repeats (SSRs) have been used to screen the allelic diversity of tetraploid Gossypium species. Fourty-seven genotypes were analyzed representing (i) the wide spectrum of diversity of the cultivated pool and
of the primitive landraces of species G. hirsutum (‘marie-galante’, ‘punctatum’, ‘richmondi’, ‘morrilli’, ‘palmeri’, and ‘latifolium’, and ‘yucatanense’), and (ii) species
G. barbadense, G. darwinii and G. tomentosum. The polymorphism of 201 SSR loci revealed 1128 allelic variants ranging from 3 to 17 per locus. Neighbor-joining (NJ) method
based on genetic dissimilarities produced groupings consistent with the assignments of accessions both at species and at race
level. Our data confirmed the proximity of the Galapagos endemic species G. darwinii to species G. barbadense. Within species G. hirsutum, and as compared to the other 6 races, race yucatanense appeared as the most distant from cultivated genotypes. Race yucatanense
also exhibited the highest number of unique alleles. The important informative heterogeneity of the 201 SSR loci was exploited
to select the most polymorphic ones that were assembled into three series of genome-wide (i.e. each homoeologous AD chromosome
pair being equally represented) and mutliplexable (× 3) SSRs. Using one of these ‘genotyping set’, consisting of 39 SSRs (one
3-plex for each of the 13 AD chromosomes pairs) or 45 loci, we were able to assess the relationships between accessions and
the topology in the genetic diversity sampled. Such genotyping set of highly informative SSR markers assembled in PCR-multiplex,
while increasing genotyping throughput, will be applicable for molecular genetic diversity studies of large germplasm collections.
Electronic Supplementary Material Supplementary material is available in the online version of this article at and is accessible for authorized users. 相似文献
4.
M. WOODHEAD J. RUSSELL J. SQUIRRELL P. M. HOLLINGSWORTH L. CARDLE M. GIBBY W. POWELL 《Molecular ecology resources》2007,7(4):631-634
A set of expressed sequence tag–simple sequence repeat (EST‐SSR) loci has been developed for Arabidopsis lyrata ssp. petraea. From 768 root cDNA clones, 126 microsatellites, including di‐, tri‐, tetra‐ and pentanucleotide repeat motifs were identified and primers were designed to 24 EST‐SSRs. Eleven loci were subsequently screened on 150 individuals sampled from five natural populations, which revealed three to nine alleles per locus (mean 5.36) and expected heterozygosity (HE) estimates ranging from 0.046 to 0.698. Significant deviations from random mating were observed at 10 EST‐SSR loci, likely due to inbreeding (global FIS = 0.151) and population structure (global FST = 0.246). 相似文献
5.
X. Wang R. Trigiano M. Windham B. Scheffler T. Rinehart J. Spiers 《Tree Genetics & Genomes》2008,4(3):461-468
Abundant, codominant simple sequence repeats (SSRs) markers can be used for constructing genetic linkage maps and in marker-assisted
breeding programs. Enrichment methods for SSR motifs were optimized with the ultimate aim of developing numerous loci in flowering
dogwood (C. florida L.) genome. Small insert libraries using four motifs (GT, CT, TGG, and AAC) were constructed with C. florida ‘Cherokee Brave’ deoxyribonucleic acid (DNA). Colony polymerase chain reaction (PCR) of 2,208 selected clones with three
primers we reported previously indicated that 47% or 1,034 of the clones harbored one of the four targeted SSR motifs. Sequencing
the putative positive clones confirmed that nearly 99% (1,021 of 1,034) of them contained the desired motifs. Of the 871 unique
SSR loci, 617 were dinucleotide repeats (70.8%), and 254 were trinucleotide or longer repeats (29.2%). In total, 379 SSR loci
had perfect structure, 237 had interrupted, and 255 had compound structure. Primer pairs were designed from 351 unique sequences.
The ability of the 351 SSR primer pairs to amplify specific loci was evaluated with genomic DNA of ‘Appalachian Spring’ and
‘Cherokee Brave’. Of these primers, 311 successfully amplified product(s) with ‘Cherokee Brave’ DNA, 21 produced weak or faint
products, and 19 did not amplify any products. Additionally, 218 of the 311 primers pairs revealed polymorphisms between the
two cultivars, and 20 out of 218 primers detected an average of 13.7 alleles from 38 selected Cornus species and hybrids. These SSR loci constitute a valuable resource of ideal markers for both genetic linkage mapping and
gene tagging of flowering dogwood.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
6.
Kishore VK Velasco P Shintani DK Rowe J Rosato C Adair N Slabaugh MB Knapp SJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(3):450-457
The Limnanthaceae (Order Brassicales) is a family of 18 taxa of Limnanthes (meadowfoam) native to California, Oregon, and British Columbia. Cultivated meadowfoam (L. alba Benth.), a recently domesticated plant, has been the focus of research and development as an industrial oilseed for three decades. The goal of the present research was to develop several hundred simple sequence repeat (SSR) markers for genetic mapping, molecular breeding, and genomics research in wild and cultivated meadowfoam taxa. We developed 389 SSR markers for cultivated meadowfoam by isolating and sequencing 1,596 clones from L. alba genomic DNA libraries enriched for AG
n
or AC
n
repeats, identifying one or more unique SSRs in 696 clone sequences, and designing and testing primers for 624 unique SSRs. The SSR markers were screened for cross- taxa utility and polymorphisms among ten of 17 taxa in the Limnanthaceae; 373 of these markers were polymorphic and 106 amplified loci from every taxon. Cross-taxa amplification percentages ranged from 37.3% in L. douglasii ssp. rosea (145/389) to 85.6% in L. montana (333/389). The SSR markers amplified 4,160 unique bands from 14 genotypes sampled from ten taxa (10.7 unique bands per SSR marker), of which 972 were genotype-specific. Mean and maximum haplotype heterozygosities were 0.71 and 0.90, respectively, among six L. alba genotypes and 0.63 and 0.93, respectively, among 14 genotypes (ten taxa). The SSR markers supply a critical mass of high-throughput DNA markers for biological and agricultural research across the Limnanthaceae and open the way to the development of a genetic linkage map for meadowfoam (x = 5).Electronic Supplementary Material Supplementary material is available in the online version of this article at
Communicated by O. Savolainen 相似文献
7.
Ana Delia Gisbert José Martínez-Calvo Gerardo Llácer María Luisa Badenes Carlos Romero 《Molecular breeding : new strategies in plant improvement》2009,23(3):523-538
Loquat [Eriobotrya japonica (Thunb.) Lindl.] is a Rosaceae fruit species of growing interest as an alternative to the main fruit crops. However, only
a few genetic studies have been carried out on this species. This paper reports the construction of the first genetic maps
of two loquat cultivars based on AFLP and microsatellite markers from Malus, Eriobotrya, Pyrus and Prunus genera. An F1 population consisting of 81 individuals, derived from the cross between ‘Algerie’ and ‘Zaozhong-6’ cultivars, was used to
construct both maps. A total of 111 scorable simple sequence repeat (SSR) loci resulted from the testing of 440 SSR primer
pairs in the analyzed progeny and the SSR transferability to Eriobotrya was found to be 74% from apple, 58% from pear and 49% from Prunus spp. In addition, 183 AFLP polymorphic bands were produced using 42 primer combinations. The ‘Algerie’ map was organized
in 17 linkage groups covering a distance of 900 cM and comprising 177 loci (83 SSRs and 94 AFLPs) with an average marker distance
of 5.1 cM. Self-incompatibility trait was mapped at the distal part of the LG17 linkage group, as previously reported in Malus and Pyrus. The ‘Zaozhong-6’ map covered 870 cM comprising 146 loci (64 SSRs and 82 AFLPs) with an average marker distance of 5.9 cM.
The 44 SSRs and the 48 AFLPs share in common by both maps were essentially collinear and, moreover, the order of the 75% of
apple and pear SSRs mapped in Eriobotrya was shown to be consistent across the Maloideae subfamily. As a whole, these maps represent a useful tool to facilitate loquat
breeding and an interesting framework for map comparison in the Rosaceae. 相似文献
8.
G. Taramino R. Tarchini S. Ferrario M. Lee M. E. Pe’ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):66-72
A total of 13 SSR loci were characterized in Sorghum bicolor. Ten of these loci were isolated by screening sorghum genomic AG-enriched libraries with labelled poly(AG)/poly(CT), the other three were derived from database searches. In order to explore the degree of polymorphism detectable in this species by this type of molecular marker, the SSR markers were tested on nine inbred lines of S. bicolor of different geographic origin. PCR analysis on acrylamide gels revealed a high degree of polymorphism (δT=0.80). One locus, in particular, allowed the identification of all of the nine inbred lines used in our study. Seven of these SSR markers were mapped, using an existing sorghum RFLP map. 相似文献
9.
Development and incorporation of microsatellite markers into the linkage map of sugar beet (Beta vulgaris spp.) 总被引:1,自引:0,他引:1
S. J. Rae C. Aldam I. Dominguez M. Hoebrechts S. R. Barnes K. J. Edwards 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(8):1240-1248
A set of informative simple sequence repeat markers has been identified for use in the marker-assisted breeding of Beta vulgaris. Highly enriched small insert genomic libraries were constructed, consisting of 1536 clones (with inserts of between 250–900
bp). Screening the clones with CA, CT, CAA, CATA and GATA nucleotide-repeat probes revealed positive hybridisation to over
50% of the clones. Of these 340 were sequenced. Primer pairs were designed for sequences flanking the repeats and, of these,
57 pairs revealed length polymorphism with 12 Beta accessions. Heterozygosity levels of the SSR loci ranged from 0.069 to 0.809. Heterozygosity levels were found to be similar
to those detected employing RFLP probes with the same accessions. Phenetic analysis using the markers, indicated relationships
in accordance with known pedigrees. Twenty three of the SSR markers were polymorphic in one or both of two F2 mapping populations, and were placed relative to a framework of RFLP probes. The markers are distributed over all nine linkage
groups of sugar beet.
Received: 14 July 1999 / Accepted: 27 October 1999 相似文献
10.
D. Dimitrova O. Georgiev C. Valkova B. Atanassova L. Karagyozov 《Biologia Plantarum》2008,52(1):149-152
Seven clones containing (CTG)n/(CAG)n repeats (n ≥ 4) were isolated by screening Lycopersicon esculentum genomic DNA. Four of the clones contained more than one simple sequence repeat (SSR). The SSRs were analyzed in several L. esculentum cultivars after polymerase chain reaction (PCR) amplification. No length variations were observed, suggesting considerable
locus stability. Five clones are from transcribed regions, which might explain the lack of cultivar variations. However the
conservation of CTG repeats was limited as differences in some transcribed loci were registered between L. pennellii and other Lycopersicon species. It is noted that in Lycopersicon trinucleotide repeat variation might be used for species identification. 相似文献
11.
Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat 总被引:39,自引:0,他引:39
Eujayl I Sorrells ME Baum M Wolters P Powell W 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):399-407
Genetic variation present in 64 durum wheat accessions was investigated by using three sources of microsatellite (SSR) markers:
EST-derived SSRs (EST-SSRs) and two sources of SSRs isolated from total genomic DNA. Out of 245 SSR primer pairs screened,
22 EST-SSRs and 20 genomic-derived SSRs were polymorphic and used for genotyping. The EST-SSR primers produced high quality
markers, but had the lowest level of polymorphism (25%) compared to the other two sources of genomic SSR markers (53%). The
42 SSR markers detected 189 polymorphic alleles with an average number of 4.5 alleles per locus. The coefficient of similarity
ranged from 0.28 to 0.70 and the estimates of similarity varied when different sources of SSR markers were used to genotype
the accessions. This study showed that EST-derived SSR markers developed in bread wheat are polymorphic in durum wheat when
assaying loci of the A and B genomes. A minumum of ten EST-SSRs generated a very low probability of identity (0.36×10−12) indicating that these SSRs have a very high discriminatory power. EST-SSR markers directly sample variation in transcribed
regions of the genome, which may enhance their value in marker-assisted selection, comparative genetic analysis and for exploiting
wheat genetic resources by providing a more-direct estimate of functional diversity.
Received: 19 December 2000 / Accepted: 17 April 2001 相似文献
12.
Development and genetic mapping of SSR markers in foxtail millet [Setaria italica (L.) P. Beauv.] 总被引:2,自引:0,他引:2
Xiaoping Jia Zhongbao Zhang Yinghui Liu Chengwei Zhang Yunsu Shi Yanchun Song Tianyu Wang Yu Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):821-829
SSR markers are desirable markers in analysis of genetic diversity, quantitative trait loci mapping and gene locating. In
this study, SSR markers were developed from two genomic libraries enriched for (GA)n and (CA)n of foxtail millet [Setaria italica (L.) P. Beauv.], a crop of historical importance in China. A total of 100 SSR markers among the 193 primer pairs detected
polymorphism between two mapping parents of an F2 population, i.e. “B100” of cultivated S. italica and “A10” of wild S. viridis. Excluding 14 markers with unclear amplifications, and five markers unlinked with any linkage group, a foxtail millet SSR
linkage map was constructed by integrating 81 new developed SSR markers with 20 RFLP anchored markers. The 81 SSRs covered
nine chromosomes of foxtail millet. The length of the map was 1,654 cM, with an average interval distance between markers
of 16.4 cM. The 81 SSR markers were not evenly distributed throughout the nine chromosomes, with Ch.8 harbouring the least
(3 markers) and Ch.9 harbouring the most (18 markers). To verify the usefulness of the SSR markers developed, 37 SSR markers
were randomly chosen to analyze genetic diversity of 40 foxtail millet accessions. Totally 228 alleles were detected, with
an average 6.16 alleles per locus. Polymorphism information content (PIC) value for each locus ranged from 0.413 to 0.847,
with an average of 0.697. A positive correlation between PIC and number of alleles and between PIC and number of repeat unit
were found [0.802 and 0.429, respectively (P < 0.01)]. UPGMA analysis revealed that the 40 foxtail millet cultivars could be grouped into five clusters in which the landraces’
grouping was largely consistent with ecotypes while the breeding varieties from different provinces in China tended to be
grouped together.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
13.
Soto-Cerda Braulio J. Carrasco Rodrigo A. Aravena Gabriela A. Urbina Hector A. Navarro Cristell S. 《Plant Molecular Biology Reporter》2011,29(3):753-759
One of the major concerns in genetic characterization and breeding of cultivated flax is the lack of informative microsatellite
markers (SSRs). In this regard, the development of SSRs using molecular methods might be time-consuming, laborious, and expensive.
On the other hand, using bioinformatics to mine sequences in public databases enables a cost-effective discovery of SSRs.
A total of 3,242 Linum usitatissimum genomic sequences were surveyed for the identification of SSRs. Among them, 118 non-redundant sequences containing repeats
were selected for designing primers. The most abundant motifs were tri- (72.4%) and dinudeotide (16.6%), within which AGG/CCT
and AG/CT were predominant. Primers were tested for polymorphism in 60 L. usitatissimum cultivars/accessions including 57 linseed and three fiber flax. Eighty-eight pairs gave amplifications within the expected
size range while 60 pairs were found to be polymorphic. The mean number of alleles amplified per primer was 3.0 (range, 2–8;
180 total alleles). The mean polymorphism information content (PIC) value was 0.39 (range, 0.06–0.87), and the highest average
PIC was observed in dinucleotide SSRs (0.41). The SSR data mining presented here demonstrates the usefulness of in silico
development of microsatellites. These novel genomic SSR markers could be used in genetic diversity studies, the development
of genetic linkage maps, quantitative trait loci mapping, association mapping, and marker-assisted selection. 相似文献
14.
S. Kresovich A. K. Szewc-McFadden S. M. Bliek J. R. McFerson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(2):206-211
A size-fractionated library of Brassica napus L. (rapeseed), composed of 15000 clones, was screened for the presence of GA-, CA-, and GATA-simple-sequence repeats (SSRs). GA-SSRs were four- and five-fold more abundant than CA- and GATA-SSRs, respectively, and present at a frequency of approximately one SSR for every 100 kb of DNA. Following the sequencing of 124 positive clones, primer pairs were designed and evaluated for seven selected SSRs. Products were amplified in an array of individuals of B. napus, B. oleracea and B. rapa, demonstrating that the seven SSRs were conserved among species. Two SSRs were polymorphic. Among 11 accessions, the dinucleotide (GA)-repeat, B.n.9A, yielded 12 fragments, while the tetranucleotide-repeat (GATA), B.n.6A2, revealed two fragments. Automated, fluorescence-based detection of polyacrylamide gels has been employed to simultaneously increase throughput, reduce unit cost, improve analytical resolution, and expedite data acquisition of SSR analysis. Though initial financial investment and technical capabilities may prevent some from directly employing our documented approach, SSR analysis warrants further investigation as a tool in genetic studies for enhancing both the conservation and utilization of genetic resources. 相似文献
15.
Rui Zhang AnDan Zhu XinJian Wang Jun Yu HongRong Zhang JiangSheng Gao YunJiang Cheng XiuXin Deng 《Plant Molecular Biology Reporter》2010,28(4):646-653
Walnut (Juglans regia), an economically important woody plant, is widely cultivated in temperate regions for its timber and nutritional fruits.
Despite abundant studies in germplasm, systemic molecular evaluations of walnut are sparsely reported mainly due to the limited
molecular markers available. Expressed sequence tags (EST) provide a valuable resource for developing simple sequence repeat
(SSR) markers. In this study, a total of 5,025 walnut ESTs (covering 16.41 Mb) were retrieved from the National Center for
Biotechnology Information database. The SSR motifs were then analyzed by the SSRHunter software. In total, 398 SSRs were obtained
with an average frequency of 1/4.08 kb. Dinucleotide (di-) repeat motifs accounted for 69.85% of all SSRs, followed by trinucleotide
(tri-) with a frequency of 27.64%, while low frequency (2.51%) of tetranucleotide (tetra-) to hexanucleotide (hexa-) was observed.
Meanwhile, GCA and TC motifs were prevalent among di- and tri- loci, respectively. Subsequently, a total of 123 primer pairs
were designed from the non-redundant SSR-containing unigenes with the selection threshold of SSR length set to 10 bp or more.
To examine the efficiency of candidate markers, seven DNA pools were collected from geographically different accessions. Results
demonstrated that 41 SSR primer sets could generate high polymorphic amplification products (33.3%), and these polymorphic
loci were mainly located in the 3′-untranslated region. Annotation analysis revealed that only two of these 41 loci were located
inside open reading frames of characterized proteins (E ≤ 1E−30). 相似文献
16.
P. Rallo G. Dorado A. Martín 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):984-989
We report the development of microsatellites or simple sequence repeats (SSRs) in the olive tree (Olea europaea L.). Forty three positive clones obtained by the screening of a GA-enriched genomic library were sequenced and primers were
designed for 13 microsatellite loci. Five primer pairs amplified polymorphic products of the expected size range. SSR polymorphism
was explored in a set of 46 olive cultivars. A total of 26 alleles were detected for the five loci. Heterozygosity ranged
from 0.46 to 0.71. Ninety one per cent of the cultivars had unique multilocus genotypes. Microsatellite segregation was studied
in a complex population from a cross between the commercial cultivars ’Leccino’ and ’Dolce Agogia’.
Received: 3 February 2000 / Accepted: 21 March 2000 相似文献
17.
S. Siju K. Dhanya S. Syamkumar B. Sasikumar T. E. Sheeja A. I. Bhat V. A. Parthasarathy 《Molecular biotechnology》2010,44(2):140-147
Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total
of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average
density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite
repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST–SSRs were developed and used for evaluating
20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated
in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this
study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus. 相似文献
18.
Dobrovolskaya O Boeuf C Salse J Pont C Sourdille P Bernard M Salina E 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(7):1145-1157
The first microsatellite linkage map of Ae. speltoides Tausch (2n = 2x = 14, SS), which is a wild species with a genome closely related to the B and G genomes of polyploid wheats, was developed
based on two F2 mapping populations using microsatellite (SSR) markers from Ae. speltoides, wheat genomic SSRs (g-SSRs) and EST-derived SSRs. A total of 144 different microsatellite loci were mapped in the Ae. speltoides genome. The transferability of the SSRs markers between the related S, B, and G genomes allowed possible integration of new
markers into the T. timopheevii G genome chromosomal maps and map-based comparisons. Thirty-one new microsatellite loci assigned to the genetic framework
of the T. timopheevii G genome maps were composed of wheat g-SSR (genomic SSR) markers. Most of the used Ae. speltoides SSRs were mapped onto chromosomes of the G genome supporting a close relationship between the G and S genomes. Comparative
microsatellite mapping of the S, B, and G genomes demonstrated colinearity between the chromosomes within homoeologous groups,
except for intergenomic T6AtS.1G, T4AL.5AL.7BS translocations. A translocation between chromosomes 2 and 6 that is present in the T. aestivum B genome was found in neither Ae. speltoides nor in T. timopheevii. Although the marker order was generally conserved among the B, S, and G genomes, the total length of the Ae. speltoides chromosomal maps and the genetic distances between homoeologous loci located in the proximal regions of the S genome chromosomes
were reduced compared with the B, and G genome chromosomes. 相似文献
19.
An integrated genetic linkage map of avocado 总被引:5,自引:0,他引:5
D. Sharon P. B. Cregan S. Mhameed M. Kusharska J. Hillel E. Lahav U. Lavi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):911-921
An avocado genomic library was screened with various microsatellite repeats. (A/T)n and (TC/AG)n sequences were found to be the most frequent repeats. One hundred and seventy-two positive clones were sequenced successfully
of which 113 were found to contain simple sequence repeats (SSR). Polymerase chain reaction primers were designed to the regions
flanking the SSR in 62 clones. A GenBank search of avocado DNA sequences revealed 1 sequence containing a (CT)10 repeat. A total of 92 avocado-specific SSR markers were screened for polymorphism using 50 offspring of a cross between the
avocado cultivars ‘Pinkerton’ and ‘Ettinger’. Both are standard avocado cultivars which are normally outcrossed and highly
heterozygous. Fifty polymorphic SSR loci, 17 random amplified polymorphic DNA (RAPD) and 23 minisatellite DNA Fingerprint
(DFP) bands were used to construct the avocado genetic map. The resulting data were analyzed with various mapping programs
in order to assess which program best accommodated data from progeny of heterozygous parents. The analyses resulted in 12
linkage groups with 34 markers (25 SSRs, 3 RAPDs and 6 DFP bands) covering 352.6 cM. This initial map can serve as a basis
for developing a detailed genomic map and for detection of linkage between markers and quantitative trait loci.
Received: 2 April 1996 / Accepted: 28 February 1997 相似文献
20.
Polymorphism of microsatellite loci of the nuclear genome was examined in 29 cultivars and accessions of wild potato (S. tuberosum, S. stoloniferum, S. demissum, and S. phureja). Nine SSR markers, most informative (PIC = 0.61–0.92) for genotyping of the cultivars of Russian breeding were selected.
Polymorphism of the selected SSR loci was characterized, and prevailing, as well as unique SSR allele phenotypes were described.
A total of 87 allele phenotypes were identified. The highest number of allele phenotypes was detected for the SSR1 (17), ST83/84 (12), and STRBCS1b (12) loci. The least numbers of allele phenotypes were typical of the ST47/48 (5) and STWIN12G (6) loci. Based on the microsatellite loci analyzed, for each of the cultivars examined, its allele formula was established.
The latter can be uses as the cultivar molecular genetic passport. Diagnostic sets of most informative loci (SSR markers),
enabling identification of the genotypes of all potato cultivars of Russian breeding examined, were determined 相似文献