The first study on the bacterial flora of the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)

The European spruce bark beetle, Dendroctonus micans Kugelann (Coleoptera, Scolytidae), is one of the most serious pests of oriental spruce (Picea orientalis L.) in Turkey. In this study, we investigated bacterial flora of D. micans collected from different populations of the forests of Eastern Black Sea Region of Turkey from 2002 to 2004. Seven different bacteria were isolated from healthy, diseased and dead specimens based on the color of colony and morphology. According to morphological, physiological and biochemical properties, metobolic enyzme profile by BIOLOG microtiter plate system, and total cellular fatty acid profile by Microbial Identification System (MIS), isolates were identified as Micrococcus luteus, Bacillus thuringiensis subsp. morrisoni, Serratia grimesii, Enterobacter cloaceae, Enterobacter intermedius, Streptococcus sp. and Pseudomonas putida. This is the first study on the bacterial flora of D. micans.     

Collembolan species diversity of calcareous canyons in the Republic of Moldova

The study of collembolan communities from the Vîşcăuți canyon in Moldova revealed 63 species belonging to 41 genera and 12 families, including four species new for the fauna of the Republic of Moldova. A checklist of collembolan species identified in the five calcareous canyons sampled so far in Moldova is included, with data on habitats, life form, occurrence and comments of distribution of most remarkable species. Of the 98 recognized species of these calcareous canyons, only 38 were shared by Vîşcăuți and the other canyons. The richness of calcareous habitats together with the high heterogeneity in faunal composition suggests that further significant increase in the species richness of the region may be expected.     

The first find of a spadefoot toad (Anura,Pelobatidae) in the Miocene of Moldova

A skeleton fragment of a spadefoot toad (family Pelobatidae Bonaparte, 1850) from the Middle Miocene (Early Sarmatian) of the Karpov Yar locality (northern Moldova) is described. Since the material is incomplete, it is only determined as Pelobatidae gen. indet. This is the first spadefoot toad from the Miocene of Moldova.     

Molecular genetic characteristics of Duchenne-Becker muscular dystrophy in the Republic of Moldova

Solution to some problems of clinical genealogical and molecular genetic study of Duchenne muscular dystrophy (DMD) in the Republic of Moldova and prenatal diagnosis aimed at preventing the birth of infants with this disease is proposed. An integrated clinical and molecular genetic study of families with a high risk of DMD has allowed its specific characteristics in the Moldovan population to be identified. The spectrum of mutations at the gene level in DMD patients and their role in prenatal and clinical diagnosis has been determined. RFLP analysis and PCR have been used to estimate the informativeness of families with a high DMD risk; prenatal diagnosis has been performed in some of them. Population analysis of the frequencies of polymorphic restriction sites have been carried out for loci pERT87-8/Tag1, pERT87-15/BamH1, and 16intron/Tag1. The results of analysis of deletion frequencies in the dystrophin gene and the frequencies of the pERT87-8, pERT87-15, and 16intron intragenic polymorphic loci have served as a basis for a strategy of molecular diagnosis. The new strategy allows the informativeness to be evaluated and, hence, clinical, preclinical, and prenatal diagnosis to be performed in approximately 94% of cases. A modified PCR method (MPCR) using the system of primers pERT87-8/Tag1 and 16intron/Tag1 has been developed for direct search for deletions. The method makes it possible to avoid diagnostic errors and decrease both the duration and the cost of the analysis.     

A first record of microsporidia in the ixodid tick Ixodes ricinus L. (Ixodidae) in the territory of the Commonwealth of Independent States, Republic Moldova

Spores of microsporidia have been recovered in 5 specimens of 13 ixodid ticks Ixodes ricinus from various populations of the Republic Moldova collected in spring of 2004. Microsporidia were detected by means of fluorescent microscopy. Intensity of infection was 3-6 spores per a micropreparate from one mite. Based on spore size, character of staining and the presence of diplocarion, these spores are referred to the Nosema-like type. Low intensity of infection probably is caused by that fact that ticks were collected in spring period and were unfed.     

隐形义齿对基牙龈沟内菌群的影响

目的将隐形义齿与传统卡环固位式可摘局部义齿进行对比,探寻隐形义齿对基牙龈沟内菌群的影响,从而指导活动义齿的选择和设计。方法下颌第一磨牙缺失的患者随机分成3组。分别戴用弯制卡环式可摘局部义齿、铸造卡环式可摘局部义齿和隐形义齿。定时对基牙龈沟进行取样并行细菌培养,并统计细菌总量、专性厌氧菌检出率以及G-菌检出率。结果戴牙后同期相比,隐形义齿组与传统卡环固位式可摘局部义齿相比,基牙龈沟内细菌总量更大,专性厌氧菌检出率及G-菌检出率更高,差异具有统计学意义。结论上述研究结果说明:相较于传统卡环固位式可摘局部义齿,隐形义齿对基牙龈沟内菌群的影响更大。对于牙周状况不佳的患者应当谨慎使用隐形义齿作为修复方式。     

Comparative study of the aerobic, heterotrophic bacterial flora of Chesapeake Bay and Tokyo Bay.

A comparative study of the bacterial flora of the water of Chesapeake Bay and Tokyo Bay was undertaken to assess similarities and differences between the autochthonous flora of the two geographical sites and to test the hypothesis that, given similarities in environmental parameters, similar bacterial populations will be found, despite extreme geographic distance between locations. A total of 195 aerobic, heterotrophic bacterial strains isolated from Chesapeake Bay and Tokyo Bay water were examined for 115 biochemical, cultural, morphological, nutritional, and physiological characters. The data were analyzed by the methods of numerical taxonomy. From sorted similarity matrices, 77% of the isolates could be grouped into 30 phena and presumptively identified as Acinetobacter-Moraxella, Caulobacter, coryneforms, Pseudomonas, and Vibrio spp. Vibrio and Acinetobacter species were found to be common in the estuarine waters of Chesapeake Bay, whereas Acinetobacter-Moraxella and Caulobacter predominated in Tokyo Bay waters, at the sites sampled in the study.     

Comparative study of the aerobic, heterotrophic bacterial flora of Chesapeake Bay and Tokyo Bay.

A comparative study of the bacterial flora of the water of Chesapeake Bay and Tokyo Bay was undertaken to assess similarities and differences between the autochthonous flora of the two geographical sites and to test the hypothesis that, given similarities in environmental parameters, similar bacterial populations will be found, despite extreme geographic distance between locations. A total of 195 aerobic, heterotrophic bacterial strains isolated from Chesapeake Bay and Tokyo Bay water were examined for 115 biochemical, cultural, morphological, nutritional, and physiological characters. The data were analyzed by the methods of numerical taxonomy. From sorted similarity matrices, 77% of the isolates could be grouped into 30 phena and presumptively identified as Acinetobacter-Moraxella, Caulobacter, coryneforms, Pseudomonas, and Vibrio spp. Vibrio and Acinetobacter species were found to be common in the estuarine waters of Chesapeake Bay, whereas Acinetobacter-Moraxella and Caulobacter predominated in Tokyo Bay waters, at the sites sampled in the study.     

Composition of the bacterial flora in tonsilloliths

Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera Eubacterium, Fusobacterium, Megasphaera, Porphyromonas, Prevotella, Selenomonas and Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor.     

Studies on the bacterial flora of tubificid worms

Study of the bacterial flora of the gut of the turbificid worms has shown that u. v. starilization, serial culturing, and standard bacterial identification procedures can be employed successfully. Eighteen species of bacteria were isolated and identified from the gut of tubificid worms. Eleven of the species were Gram negative which may be reflected in their possible association with chlorogonal cell metabolism in tubificid worms. Of the eighteen species identified our genera and two species are on a similar list produced by Brinkhurst & Chua in an earlier study (1969) of worms from the Great Lakes. None of the organisms identified were other than common to fresh water, organic decay processes or animal digestive systems. Their role in tubificid nutrition is now under study.Supported by a grant from the Council on Faculty Research, Eastern Illinois University.     

转crylAb基因克螟稻对根际可培养细菌类群的影响

田间栽培条件下,研究了转crylAb基因克螟稻1(KMD1)、克螟稻2(KMD2)及其亲本常规晚粳秀水11(XS11)不同生育期、不同根际部位(包括根际土壤、根表面和根内)可培养细菌类群数量、组成和动态变化.结果表明,各品种不同生育期根际平均细菌数量以拔节期最多,抽穗期最少;转Bt稻KMD1和KMD2根际平均细菌数量均显著高于XS11,且根表面和根内平均细菌数量也显著高于XS11,而根际土壤中平均细菌数量则低于XS11;试验共纯化菌株303个,经鉴定归于20个属,各品种根际出现的细菌类群大多为相同属,但数量有异,其中KMD1、KMD2根际的葡萄球菌属(Staphylococcus)、棒杆菌属(Corynebacterium)和壤霉菌属(Agromyces)均显著高于XS11,而XS11根际的束毛球菌属(Trichococcus)显著高于KMD1、KMD2;群落多样性分析表明,各水稻品种根际细菌群落的优势度指数C、多样性指数H′和均匀度指数J差异均不显著,表明转crylAb基因水稻对土壤细菌群落有一定影响,与水稻生育期和根际部位有关.     

The effects of endometrial scarification on uterine steroid receptors, bacterial flora and histological structure in the bitch.

Following laparotomy, the endometrium of six nulliparous Beagle bitches was scarified at the base of one uterine horn during early metoestrus, when the peripheral plasma P(4) concentration was >10 ng/ml; intrauterine swabs were taken at the same time for bacteriological culture. Twenty-one days later, a bilateral ovariohysterectomy was performed and segments of the scarified and non-scarified parts of the tubular genital tract removed; at the same time, swabs were taken from the uterine lumen. Tissue samples were collected and examined for histopathological structure, and the presence of nuclear oestrogen (ER) and progesterone (PR) receptors using an immunocytochemical method. The immunoreactivity was scored semiquantitatively, incorporating both the intensity and distribution of specific staining of the receptors using a simplified histoscore (H-score).All uterine swabs were sterile, and in three of the six bitches there were noticeable changes with distension of the uterine lumen with secretions and debris and distension of the endometrial gland ducts of the scarified uterine segment. There were no statistically significant differences in the H-scores of ER or PR between scarified and non-scarified segments, except for PR H-scores in the glandular epithelium where the values for the scarified were significantly higher than for the non-scarified endometrium (mean+/-S.E.M. is 129.9+/-22.8 versus 59.5+/-12.6; P<0.05). Thus, trauma can modify the structure of the endometrium and the characteristics of the PR. Whether changes in PR expression are involved in the pathogenesis of CEH/pyometra in the bitch could not be ascertained from this study.     

Note on the bacterial flora of the Egyptian desert in summer

Summary The response of bacterial population to edaphic drought of the Egyptian desert in summer has been investigated. The sporeformers Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium have predominated in the soil. B. subtilis and B. licheniformis predominated in the rhizosphere of the studied chamaephytes. The total bacterial counts were found to be much higher in the rhizospheres than in the soils apart. Azotobacter sp. and Clostridium sp. have been isolated from rhizospheres but not from soils. The significance of the rhizosphere as constituting a microhabitat in the desert community, has been discussed. The probable contribution of the peritrophic mantle of bacteria for protecting root cells against edaphic drought has also been notified.