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1.
Cellular Microcystin Content in N-Limited Microcystis aeruginosa Can Be Predicted from Growth Rate 总被引:1,自引:0,他引:1
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Cell quotas of microcystin (QMCYST; femtomoles of MCYST per cell), protein, and chlorophyll a (Chl a), cell dry weight, and cell volume were measured over a range of growth rates in N-limited chemostat cultures of the toxic cyanobacterium Microcystis aeruginosa MASH 01-A19. There was a positive linear relationship between QMCYST and specific growth rate (μ), from which we propose a generalized model that enables QMCYST at any nutrient-limited growth rate to be predicted based on a single batch culture experiment. The model predicts QMCYST from μ, μmax (maximum specific growth rate), QMCYSTmax (maximum cell quota), and QMCYSTmin (minimum cell quota). Under the conditions examined in this study, we predict a QMCYSTmax of 0.129 fmol cell−1 at μmax and a QMCYSTmin of 0.050 fmol cell−1 at μ = 0. Net MCYST production rate (RMCYST) asymptotes to zero at μ = 0 and reaches a maximum of 0.155 fmol cell−1 day−1 at μmax. MCYST/dry weight ratio (milligrams per gram [dry weight]) increased linearly with μ, whereas the MCYST/protein ratio reached a maximum at intermediate μ. In contrast, the MCYST/Chl a ratio remained constant. Cell volume correlated negatively with μ, leading to an increase in intracellular MCYST concentration at high μ. Taken together, our results show that fast-growing cells of N-limited M. aeruginosa are smaller, are of lower mass, and have a higher intracellular MCYST quota and concentration than slow-growing cells. The data also highlight the importance of determining cell MCYST quotas, as potentially confusing interpretations can arise from determining MCYST content as a ratio to other cell components. 相似文献
2.
Hongbin Liu Edward A. Laws† Tracy A. Villareal ¶ Edward J. Buskey¶ 《Journal of phycology》2001,37(4):500-508
Growth and chemical compositional characteristics of the brown tide-forming alga, Aureoumbra lagunensis Stockwell, DeYoe, Hargraves et Johnson, were studied through a series of nitrogen-limited and phosphate-limited continuous cultures over a range of growth rates. The specific growth rate of A. lagunensis was hyperbolically related to the cell quota of the limiting nutrient in ammonium-limited cultures. In phosphate-limited cultures, the relationship was best described by a straight line. The N cell quota of A. lagunensis ranges from about 20 fmol at zero growth rate under N-limited conditions to a high of roughly 85 fmol under N-replete conditions. Similarly, the P cell quota of A. lagunensis ranges from about 0.15 fmol at zero growth rate under P-limited conditions to a high of 2 fmol under P-replete conditions. Aureoumbra lagunensis has a very high N:P critical ratio (>100). The high N:P critical ratio, as well as the organism's apparent ability to use forms of phosphorus other than phosphate under severe phosphate deficiency, may partially explain its success in P-limited environments, such as the Laguna Madre. In addition, a uniqe quadratic relationship between the productivity index (PI) and growth rate was discovered. Such a relationship supports an earlier argument that PI may not be a good indicator of nutritional status. 相似文献
3.
Theoretical considerations predict that the cell N:P ratio at transition from nitrogen limitation to phosphorus limitation of phytoplankton growth (critical ratio, Rc) varies, as a function of population growth rate. This prediction is confirmed by experimental, data from the literature along with new experimental data for the marine, prymnesiophyte Pavlova lutheri (Droop) Green. Rc passes through a maximum at intermediate growth rates for the three phytoplankton species for which data, are available, but there is significant interspecific variability in its value. There is no theoretical or experimental evidence to support the idea that the ratio of subsistence N and P cell quotas is equal to Rc over the range of growth rates, or that the subsistence quota ratio equals the ratio of the N and P cell quotas minus a storage fraction. Examination of N:P composition ratios can be used to determine which nutrient is limiting, but cannot be used to determine relative growth rates or competitive advantage between species limited by the same nutrient. Growth rates are determined by environmental conditions and by the cell quota of the limiting nutrient, without reference to the cell quota of the non-limiting nutrient. 相似文献
4.
The specific uptake rate Q(p) of orthophosphate (expressed throughout as phosphorus) and the specific growth rate mu of Microcystis aeruginosa were measured using batch-precultured cells, whose growth phase, and intracellular and extracellular phosphorus concentrations f(p) and P, respectively, had been changed. When the cells from phosphorus-rich precultures were used, smaller values of Q(p) (0.1-0.3 mug P mg dry wt. (-1) h (-1)) were observed. However, if phosphorusstarved cells were used, the initial value of Q(p) was enhanced to more than ten times those smaller values referred to above, but declined rapidly with time after the transfer. Q(p) leveled off at around t = 4 h, when f(p) approached the maximum value, even if phosphorus was still available in the medium. A new correlation was presented here with respect to Q(p) as a function of P and f(p) as follows: \documentclass{article}\pagestyle{empty}\begin{document}$$ Q_p = Q_{p,\max } \frac{P}{{K_p + P}}\frac{{(f_{p,\max } - f_p )}}{{(f_{p,\max } - f_p )}} $$\end{document} Although numerical values of parameters involved in the equation depend on physiological state (or preculture history) of the cells, the above equation could account not only for phosphorus uptake, during which changes in phosphorus content in the cells were observed, but also for initial rates of uptake presented previously by other workers. mu Values were confirmed to be a hyperbolic function of f(p) as has been suggested by previous workers. 相似文献
5.
Impact of inorganic carbon availability on microcystin production by Microcystis aeruginosa PCC 7806
Jähnichen S Ihle T Petzoldt T Benndorf J 《Applied and environmental microbiology》2007,73(21):6994-7002
Batch culture experiments with the cyanobacterium Microcystis aeruginosa PCC 7806 were performed in order to test the hypothesis that microcystins (MCYSTs) are produced in response to a relative deficiency of intracellular inorganic carbon (C(i,i)). In the first experiment, MCYST production was studied under increased C(i,i) deficiency conditions, achieved by restricting sodium-dependent bicarbonate uptake through replacement of sodium bicarbonate in the medium with its potassium analog. The same experimental approach was used in a second experiment to compare the response of the wild-type strain M. aeruginosa PCC 7806 with its mcyB mutant, which lacks the ability to produce MCYSTs. In a third experiment, the impact of varying the C(i,i) status on MCYST production was examined without suppressing the sodium-dependent bicarbonate transporter; instead, a detailed investigation of a dark-light cycle was performed. In all experiments, a relative C(i,i) deficiency was indicated by an elevated variable fluorescence signal and led to enhanced phycocyanin cell quotas. Higher MCYST cell quotas (in the first and third experiments) and increased total (intracellular plus extracellular) MCYST production (in the first experiment) were detected with increased C(i,i) deficiency. Furthermore, the MCYST-producing wild-type strain and its mcyB mutant showed basically the same response to restrained inorganic carbon uptake, with elevated variable fluorescence and phycocyanin cell quotas with increased C(i,i) deficiency. The response of the wild type, however, was distinctly stronger and also included elevated chlorophyll a cell quotas. These differences indicate the limited ability of the mutant to adapt to low-C(i,i) conditions. We concluded that MCYSTs may be involved in enhancing the efficiency of the adaptation of the photosynthetic apparatus to fluctuating inorganic carbon conditions in cyanobacterial cells. 相似文献
6.
Ten species of marine phytoplankton were grown under a range of photosynthetic photon flux densities (PFDs) and examined for variation in cell volume and carbon quota. Results suggest that in response to low PFDs phytoplankton generally reduce their cell volume and frequently reduce their carbon quota. A significant linear relationship between the log of PFD (I) and cell volume (in nine of ten species) and log I and carbon quota (four of ten species) was demonstrated. When exposed, to a transient in light intensity, Thalassiosira pseudonana (Hustedt, clone 3H) Hasle and Heimdal underwent a rapid adaptation in cell volume and carbon quota. Cells going from low light to high light reached maximum mean cell volume within 5 h, and cells going from high light to low light reached a minimum mean cell volume within 12 h. The resulting kinetic constant (k; a measure of the rate of adaptation) was considerably larger than previously reported k values. Ditylum brightwellii (West) Grunow increased in length but did not increase in width during a transient to increased irradiance. Nutrient limitation was shown to override PFD in determining cell volume and carbon quota for Heterosigma akashiwo Hada. Cells grown at equivalent irradiances but N-limited, were smaller than light-limited and nutrient-saturated cells. Therefore, cell volume and carbon quota do not have the same relationship with PFD when factors other than PFD control growth rate. The ecological implications of reduced cell volumes and carbon quotas with decreasing PFD include possible impacts on CO2 budgets, an influence on sinking rates, potential changes in predation rates, and surface area/cell volume benefits. 相似文献
7.
Comparative physiology of two protozoan parasites, Leishmania donovani and Trypanosoma brucei, grown in chemostats.
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Cultures of the insect stage of the protozoan parasites Leishmania donovani and Trypanosoma brucei were grown in chemostats with glucose as the growth rate-limiting substrate. L. donovani has a maximum specific growth rate (mu max) of 1.96 day-1 and a Ks for glucose of 0.1 mM; the mu max of T. brucei is 1.06 day-1 and the Ks is 0.06 mM. At each steady state (specific growth rate, mu, equals D, the dilution rate), the following parameters were measured: external glucose concentration (Glcout), cell density, dry weight, protein, internal glucose concentration (Glcin), cellular ATP level, and hexokinase activity. L. donovani shows a relationship between mu and yield that allows an estimation of the maintenance requirement (ms) and the yield per mole of ATP (YATP). Both the ms and the YATP are on the higher margin of the range found for prokaryotes grown on glucose in a complex medium. L. donovani maintains the Glcin at a constant level of about 50 mM as long as it is not energy depleted. T. brucei has a decreasing yield with increasing mu, suggesting that it oxidizes its substrate to a lesser extent at higher growth rates. Glucose is not concentrated internally but is taken up by facilitated diffusion, while phosphorylation by hexokinase is probably the rate-limiting step for glucose metabolism. The Ks is constant as long as glucose is the rate-limiting substrate. The results of this study demonstrate that L. donovani and T. brucei have widely different metabolic strategies for dealing with varying external conditions, which reflect the conditions they are likely to encounter in their respective insect hosts. 相似文献
8.
Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate Isochrysis galbana to carbon or nitrogen stress 总被引:1,自引:0,他引:1
The growth of the marine flagellate Isochrysis galbana was followed in batch cultures at four concentrations of dissolved inorganic carbon (DIC), from C- and N-replete lag phase into C- and/or N-deplete stationary phase. Organic buffers were omitted from the growth medium, and culture pH was maintained at 8.30±0.05 by the addition of acid or alkali. The responses of the flagellate to N stress included an increase in the C:N ratio, and decreases in the ratios of glutamine (Gln):glutamate (Glu) and Chl a :C, and the cell Chl a quota. Conversely, the responses to C stress included a decrease in the C:N ratio, and increases in the ratios of Gln:Glu and Chl a :C, and the cell Chl a quota. The relationship between carbon-specific growth rate (C-μ), and the concentration of extracellular DIC, [DIC]ext , exhibited Michaelis–Menten type kinetics with a half saturation constant, K G(DIC) , of 81 μM. Comparative studies of the diatom Phaeodactylum tricornutum showed similar results, although the value of K G(DIC) was lower at 30 μM. 相似文献
9.
Temperature effects on steady-state growth,phosphorus uptake,and the chemical composition of a marine phytoplankter 总被引:1,自引:0,他引:1
Joel C. Goldman 《Microbial ecology》1979,5(3):153-166
The marine chrysophyteMonochrysis lutheri was grown in phosphorus-limited continuous cultures at temperatures of 15°, 18.8° and 23°C. The effect of temperature on the maximum growth rate was well-defined by the Arrhenius equation, but the Q10 for this alga (1.7) was somewhat lower than has been determined previously for many other phytoplankton species (2.0–2.2). The minimum phosphorus cell quota was relatively unaffected by temperature at 18.8°C and 23°C, but doubled in magnitude at 15°C. As a result, the internal nutrient equation of Droop described the relationship between specific growth rate and phosphorus cell quota well at 18.8° and 23°C, but was less successful at 15°C. The major limitation in using the Droop equation is that the ratio between the minimum and maximum cell quotas must be known, thus necessitating the need to establish the true maximum growth rate by the cell washout technique. In addition, the phosphorus uptake rate on a cell basis at a given steady state growth rate (=specific uptake rate) increased dramatically at 15°C, whereas the turnover rate of total available phosphorus was unaffected by temperature. Both the nitrogen and carbon cell quotas were relatively unaffected by growth rate at a given temperature, but the average values increased slightly with decreasing temperature. The overall conclusion is that phytoplankton growth and limiting-nutrient uptake rates are only synchronous at or near the optimum temperature. Because these types of responses are species specific, much additional data on temperature effects will be required before the importance of including such effects in phytoplankton-nutrient models can be determined. 相似文献
10.
Zoltan Mastala Sndor Herodek Katalin V.-Balogh Gyrgy Borbly Hesham Mohamed Shafik Lajos Vrs 《International Review of Hydrobiology》1996,81(4):503-512
A pico sized Synechococcus species isolated from Lake Balaton was studied in batch and continuous cultures. This picocyanobacterium had a pH optimum at 8.5 and a temperature optimum at 28-30°C. The Ik value for growth was 52 μEinstein m−2 S−1, the maximum growth rate 2.27 d−1, the half saturation Constant of growth 1.2 μg PO4-P I−1 and the minimal cell quota 1.74 nig P g dry weight−1. The dry weight of cells showed a minimum, the chlorophyll-a/biomass ratio a maximum as a function of growth rate. Above the quota of 3.4 fg P Cell−1 significant amounts of non-reactive dissolved Phosphorous were released. 相似文献
11.
Optimal production of glutathione by controlling the specific growth rate of yeast in fed-batch culture 总被引:8,自引:0,他引:8
The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile of mu was calculated as a bang-bang type, That is mu, should start from the maximum value, mu(max) and should be kept at mu(max); then mu should be switched to mu(c), which gives a maximum value of rho(G). It was proven from the maximum principle that switching was needed only once, with the switching time from mu(max) to mu(c) depending on the final required glutathione content. Finally, this ideal profile of mu for the maximum production of glutathione was realized by manipulating the substrates feed rate in the fed-batch culture. Using the extended Kalman filter and a programmed-controller/feedback-compensator (PF) system, mu could be controlled at the optimal profile obtained. As a result, the maximum production of glutathione was accomplished fairly successfully. However, further improvement in the controller performance for mu is desired. The control strategy employed here can be applied to other batch reaction processes. 相似文献
12.
Transitions in the growth limiting factor from light (I) to nitrogen (N) and vice versa caused changes in geosmin production, protein and carbohydrate content, and the synthesis of pigments such as chlorophyll a (Chl a), phycobiliproteins (PBPs), and -carotene of the cyanobacterium Oscillatoria brevis. Following IN transition the first 150h, the decrease in protein content was compensated for by an increase of carbohydrates, and thereby, a constant biomass level was maintained in this period. Thereafter, biimass dropped to 15% of its initial level. A decrease in geosmin and pigment content was observed during transition from IN-limited growth. However, geosmin increased relative to phytol (Chl a) and -carotene which may indicate that a lowered demand for phytol and -carotene during N-limited growth allows isoprenoid precursors to be directed to geosmin rather than to pigment synthesis. Synthesis of Chl a and -carotene at the expense of geosmin was suggested for the observed start of increase in geosmin production only at the time that Chl a and -carotene had reached their I-limited steady state. Transition from nitrogen to light limited growth caused an acceleration of metabolism shown by a rapid decrease in carbohydrate content accompanied by an increase in protein content. The growth rate of the organisms temporarily exceeded the dilution rate of the culture and the biomass level increased 6-fold. Due to the only modest changes in geosmin production (2-fold) compared to changes in biomass level (6-fold) during I-or N-limited growth, environmental factors seem to have limited effect on geosmin production.Abbreviations Chl a
chlorophyll a
- dry wt
dry weight;
- I-limited
light-limited
- N-limited
nitrogen-limited
- PBP
phycobiliprotein
This research was performed at the Department of Microbiology, University of Amsterdam, with finacial support provided by the Royal Norwegian Ministry of Foreign Affairs and the Royal Norwegian Council for Scientific and Industrial Research 相似文献
13.
Selenastrum minutum (Naeg.) Collins was grown over a wide range of growth rates under phosphate or nitrate limitation with non-limiting nutrients added to great excess. This resulted in saturated luxury consumption. The relationships between growth rate and cell quota for the limiting nutrients were well described by the Droop relationship. The observed variability in N cell quota under N limitation as reflected in kQ·Qmax?1*, was similar in magnitude to previously reported values but kQ·Qmax?1* for P under P limitation was greater than previously reported for other species. These results were evaluated in light of the optimum ratio hypothesis. Our findings support previous work suggesting that the use of a single optimum ratio (kQi·KQj?1) is inappropriate for dealing with a species growing under steady-state nutrient limitation. Under these conditions the optimum ratio should be viewed as a growth rate dependent variable. Two approaches for testing the growth rate dependency of optimum ratios are proposed. The capacity for luxury consumption differed between nutrients and was growth rate dependent. At low growth rates, the coefficient of luxury consumption (Rsat) for P was ca. four times that for N. The set of all possible relationships between N and P cell quota under these conditions was reported and these values were then used to establish the cellular N:P niche boundaries for S. minutum. Cell quotas of non-limiting nutrients were not described by the Droop equation. Analysis showed that as the cellular N:P ratio deviates from the optimum ratio, the ability of the Droop equation to describe the relationship between growth rate and non-limiting cell quotas decreases. When non-limiting nutrient cell quotas are saturated, the Droop equation appears to be invalid. Previously reported patterns of non-limiting nutrient utilization are summarized in support of this conclusion. The physiological and ecological consequences of luxury consumption and growth rate dependent optimum ratios are considered. 相似文献
14.
重组巴氏毕赤酵母恒化培养动力学及代谢迁移特性研究 总被引:5,自引:0,他引:5
通过对甲醇营养型毕赤酵母基因工程菌以碳源甘油为限制性基质进行恒化培养动力学试验 ,结果认为 :(1 )细胞光密度与其干、湿重呈线性关系 ,当细胞光密度 (OD60 0 )为 1 0 0时细胞湿重 (WCW)为 1 2 8 3g L ,细胞干重 (WDW)则为 2 2 9g L ;(2 )基因工程菌P .pastoris的生长与限制性基质甘油残留浓度的关系符合Monod关系式 ,通过 1 μ对 1 S进行线性回归得 μmax=0 .366h- 1,Ks=0 .1 82 3g L ,经参数推导甘油最大菌体得率系数YG =0 .54g g ,菌体维持生长消耗底物系数m =0 .0 0 69g (g·h) ;氧最大菌体系数YX O2 =30 .96g moL ,菌体维持生长时消耗氧系数mO2 =0 .0 0 0 8mol (g·h) ,最适理论稀释速率Dm =0 .341h- 1;(3)从氨水的消耗速率和呼吸商 (RQ)的变化认为随着比生长速率 (μ)的增大 ,甘油代谢流从糖原异生和磷酸戊糖途径线性地向糖酵解和三羧酸循环途径进行代谢迁移 ,即糖酵解和三羧酸循环途径的代谢流量在线性地增大 相似文献
15.
High cell density cultivation of Escherichia coli at controlled specific growth rate. 总被引:7,自引:0,他引:7
D Riesenberg V Schulz W A Knorre H D Pohl D Korz E A Sanders A Ross W D Deckwer 《Journal of biotechnology》1991,20(1):17-27
A high cell density cultivation (HCDC) for growth of Escherichia coli in an especially designed glucose/mineral salt medium is proposed. The HCDC essentially starts as a batch process which is followed by a two-phase fed-batch cultivation. After unlimited growth at mu max = 0.45 h-1 in the batch part, growth was controlled at a reduced specific growth rate (mu = 0.11 h-1 less than mu max) over a period of 3 doubling times in which the biomass concentration increased from 12 to 95 g 1(-1) (phase 1 of fed-batch cultivation). Control of growth (mu) was realized by a PO2 control loop (by variation of glucose feeding) and a mu control loop (by variation of agitation speed N) while the actual mu was calculated from the off-gas composition. If the agitation rate cannot be increased anymore the mu controller is switched off (end of phase 1). In the following phase 2, mu declines, however, the still acting pO2 (glucose) controller guarantees sufficient O2 supply till the end of the cultivation with a biomass concentration of 110 g 1(-1) (dry mass). The proposed HCDC suppresses generation of inhibitory by-products and the high yield coefficients indicate the economy of the process. 相似文献
16.
Relationship between astaxanthin production and the intensity of anabolic processes in the yeast Phaffia rhodozyma 总被引:1,自引:0,他引:1
The astaxanthin synthesis in the yeast Phaffia rhodozyma was shown to depend on the rate of growth occurring in the first two days of cultivation. The growth rate of the yeast culture studied was preset by the cultivation conditions, among which the C:N ratio was decisive. The intense anabolic processes coupled with active culture growth during the first 24 h significantly inhibited the synthesis of the key enzymes involved in astaxanthin synthesis, which led to a marked decrease in the carotenoid production. It was demonstrated that for the maximum yield of astaxanthin to be obtained from 11 of nutrient medium, it is necessary to carry out cultivation, beginning with the first day, at a growth rate significantly lower than mu(max). The optimum budding rate of the mutant strain Ph. rhodozyma VKPM Y-2409 consistent with the maximum astaxanthin synthesis was determined. The specific astaxanthin productivity of the strain studied was about 7.0 mg/g of dry biomass at a budding rate of <0.5. 相似文献
17.
Costa JA de Morais MG Dalcanton F Reichert Cda C Durante AJ 《Zeitschrift für Naturforschung. C, Journal of biosciences》2006,61(1-2):105-110
Mangueira Lagoon, located in the extreme south of Brazil, has water with physicochemical characteristics such as alkaline pH and carbonate levels propitious for the growth of the cyanobacterium Spirulina platensis. Previously published studies have shown that Mangueira Lagoon water supplemented with small quantities of carbon and nitrogen is suitable for S. platensis cultivation and can significantly reduce production costs. We studied mixed cultures of Spirulina platensis and the toxic cyanobacterium Microcystis aeruginosa using a 2(3) factorial design in which the three factors were the initial biomass concentration of S. platensis and M. aeruginosa and the type of culture medium (100% Zarrouk's medium or 80% Mangueira Lagoon water plus 20% Zarrouk's medium). The highest S. platensis maximum specific growth rate (mu(max)) occurred in the culture with the highest M. aeruginosa biomass concentration and when undiluted culture medium was used (micro(max) = 0.283 d(-1)). The highest M. aeruginosa specific death rate (k) was obtained in the presence of S. platensis (k = 0.555 d(-1)) and was independent of the initial M. aeruginosa biomass concentration and culture medium, demonstrating that S. platensis cultures are not susceptible to contamination by M. aeruginosa. The culture medium had no significant influence (p > 0.05) on S. platensis micro(max) values, indicating that production costs could be reduced by using a medium consisting of 80% Mangueira Lagoon water plus 20% Zarrouk's medium. 相似文献
18.
Effect of elevated dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum on D-glucose and L-lactate 总被引:1,自引:0,他引:1
Bäumchen C Knoll A Husemann B Seletzky J Maier B Dietrich C Amoabediny G Büchs J 《Journal of biotechnology》2007,128(4):868-874
The effect of increased dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum was studied with continuous turbidostatic cultures. The carbon sources were either l-lactate or d-glucose. To increase the dissolved carbon dioxide concentration the carbon dioxide partial pressure of the inlet gas stream pCO2,IN was increased stepwise from 0.0003 bar (air) up to 0.79 bar, while the oxygen partial pressure of the inlet gas stream was kept constant at 0.21 bar. For each resulting carbon dioxide partial pressure pCO2 the maximum specific growth rate mu(max) was determined from the feed rate resulting from the turbidostatic control. On d-glucose and pCO2 up to 0.26 bar, mu(max) was mostly constant around 0.58 h(-1). Higher pCO2 led to a slight decrease of mu(max). On l-lactate mu(max) increased gradually with increasing carbon dioxide partial pressures from 0.37 h(-1) under aeration with air to a maximum value of 0.47 h(-1) at a pCO2 of 0.26 bar. At very high pCO2 (0.81 bar) mu(max) decreased down to 0.35 h(-1) independent of the carbon source. 相似文献
19.
Seven strains of 2,4-dichlorophenoxyacetic acid-degrading bacteria, including Pseudomonas, Alcaligenes, and Bordetella spp., were compared on the basis of growth kinetics. Estimates of maximum growth rate (mu max, k1) and half-saturation growth constant (Ks, k3) were obtained by fitting substrate depletion curves to a four-parameter version of the integrated Monod equation. Estimates of Ks ranged from 2.2 micrograms/ml (10 microM) to 33.8 micrograms/ml (154 microM), and estimates of mu max ranged from 0.20 h-1 (Td = 3.5 h) to 0.32 h-1 (Td = 2.2 h). Estimates of mu max, but not Ks, were affected by changes in initial inoculum density. Maximum growth rates (mu max) were also estimated from turbidity measurements. They ranged from 0.10 h-1 (Td = 6.9 h) to 1.0 h-1 (Td = 0.7 h). There was no correlation between estimates of mu max derived from substrate depletion curves and those derived from turbidity measurements (P = 0.20). 相似文献
20.
Kinetic comparison of seven strains of 2,4-dichlorophenoxyacetic acid-degrading bacteria. 总被引:6,自引:6,他引:0
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Seven strains of 2,4-dichlorophenoxyacetic acid-degrading bacteria, including Pseudomonas, Alcaligenes, and Bordetella spp., were compared on the basis of growth kinetics. Estimates of maximum growth rate (mu max, k1) and half-saturation growth constant (Ks, k3) were obtained by fitting substrate depletion curves to a four-parameter version of the integrated Monod equation. Estimates of Ks ranged from 2.2 micrograms/ml (10 microM) to 33.8 micrograms/ml (154 microM), and estimates of mu max ranged from 0.20 h-1 (Td = 3.5 h) to 0.32 h-1 (Td = 2.2 h). Estimates of mu max, but not Ks, were affected by changes in initial inoculum density. Maximum growth rates (mu max) were also estimated from turbidity measurements. They ranged from 0.10 h-1 (Td = 6.9 h) to 1.0 h-1 (Td = 0.7 h). There was no correlation between estimates of mu max derived from substrate depletion curves and those derived from turbidity measurements (P = 0.20). 相似文献