THE FROND OF HETERANGIUM

Frond members belonging to the monostelic seed fern genus Heterangium have been found in Pennsylvanian age coal balls collected in the Illinois Basin. Petioles bear small pinnae below a dichotomy which produced a bipartite frond. Pinnules of the Sphenopteris obtusiloba type are borne on secondary pinnae. The anatomy of each pinna order and the pinnules is described.     

Interaction Between Cryopreservation, Rewarming Rate and Seed Humidification on the Germination of Two Spanish Endemic Species

The effects of humidification, storage in liquid nitrogen (1or 30 d) and rewarming rate on seed germination were studiedin two Spanish endemics. Humidification resulted in higher germinationpercentages only in the species with hard covers, especiallyin slowly rewarmed seeds. In an experiment lasting 21 weeks,seeds stored in liquid nitrogen were removed for 10 min eachweek to mimic the withdrawal of samples from a seed bank; thishad no effect on germination.Copyright 1998 Annals of BotanyCompany Centaurea hyssopifolia,Limonium dichotomum, cryopreservation, cypsela, endemics, germination, humidification, seeds.     

Anomalous branching patterns in some fossilFilicales: Implications in the evolution of the megaphyll and the lateral branch,habit and growth pattern

From middle Carboniferous coal balls leaves and stems of structurally preserved Filicalean ferns (Psalixochlaenaceae, Botryopteridaceae) are described that deviate from the normal pattern of branching. From this, interpretations are made as to the origin and relationship of axillary branching and dichotomy in this line of ferns. Their megaphylls apparently have orginated from a three-dimensional dichotomous branching system. This is inferred from the widespread occurrence of short, leafy epiphyllous buds on their fronds and is related to their habit where the leaf often plays the dominant role and effects vegetative propagation.     

ETAPTERIS LECLERCQII SP. N., A ZYGOPTERID FERN FROM THE PENNSYLVANIAN OF NORTH AMERICA

The zygopterid fern Etapteris leclercqii sp. n. is described from Lower Pennsylvanian age coal balls from the Lewis Creek, Kentucky, locality. Isolated petioles extend up to 15.0 cm in length and 5.0 mm in diam; no lateral axes have been observed. The clepsydroid-shaped petiole trace is characterized by lateral arms that sharply taper and by the production of peripheral loops prior to the separation of pinna traces from the stele. Large irregularly shaped multicellular hairs are randomly scattered over the surface of the petioles. When compared with other currently recognized zygopterid ferns, E. leclercqii appears most similar to Metaclepsydropsis duplex. Based on the configuration of the leaf trace and the occurrence of peripheral loops, it is suggested that E. leclercqii may represent the most primitive species of the genus known to date.     

Aspects of the Comparative Physiology of Ranunculus bulbosus L. and Ranunculus repens L.: I. Response to Nitrogen

Ranunculus bulbosus and R. repens are closely related taxonomicallyand coexist in the same general habitat but differ in that R.bulbosus only exceptionally shows vegetative multiplicationcontrasting with R. repens which reproduces extensively by meansof long branched stolons which root and form new daughter plantsat the nodes. The response of these two species to nitrogen was similar inthat an increase from 10 to 50 ppm nitrogen gave rise to greatlyincreased total dry weight and higher shoot to root ratios,whereas further nitrogen increase from 50 to 200 ppm had littleeffect. However, they differed in that at low nitrogen levelsthe perennating organ (corm) took priority in R. bulbosus withsexual reproduction assuming greater importance at the highernitrogen levels, whereas in R. repens the main storage organ(the stock) was not especially favoured at low nitrogen levelsreflecting perhaps the differences between this structure andthe corm in R. bulbosus. In R. repens the greatest responseto nitrogen was shown by the stolons although this was due toincreased branching and a higher dry weight per unit lengthat the higher levels of nitrogen (50 and 200 ppm). Primary stolonlength was not greatly affected by nitrogen level. Dry weightsof flowers and fruits in R. repens were very much lower thanin R. bulbosus. It is suggested that in R. bulbosus the emphasis lies firstin replacement of the parent at approximately the same siteby a daughter from the new corm and secondly in sexual reproductivespread. In R. repens lateral spread by vegetative propagationtakes precedence over all else and under low nitrogen conditionsbranching is sacrificed and energy is diverted to maintaininglong primary stolons. These, in turn, have the potential forproducing daughter plants at some distance from the parent andthis may be of considerable importance in field situations sincethe daughter may come to occupy more favourable sites than theparent.     

Prochloron (Prochlorophyta): Biochemical Contributions to the Chlorophyll and RNA Composition

The prochlorophyte Prochloron, a symbiont of the colonial ascidianDidemnum molle, was collected in the Indian Ocean around Giravaru(Maldives) in depths between 1 and 40 m. The chlorophyll a tob ratio of the algal symbionts was higher in colonies livingbetween 1–6 m, compared to that determined for Prochloronfrom a depth of 30 m. This property for chromatic adaptationin correlation with changes in the total content of chlorophyllis dependent upon environmental factors. The association betweenDidemnum and Prochloron is only a facultative symbiosis. Thesize of the colonies, growing near the water surface is large(up to 3 cm), and it gradually decreases to 0.2 cm in a depthof 30 m dim locations. At a depth of 40 m the tunicates do notcontain the algal symbionts. Applying quantitative preparative isolation and sensitive immunologicalas well as biochemical detection techniques we have no evidencefor the existence of poly(A) stretches in RNA species from Prochloron.Moreover, we failed to detect both sn/scRNAs and their proteins,typically associated with them in RNP complexes from eukaryotes.From the data we suggest that mRNA synthesis proceeds in Prochloronin a way similar to prokaryotes. ¹ This contribution is dedicated to Prof. Dr. Dr. B. Schmidton the occasion of his 50th birthday.     

The Development of Fast-Start Performance in Fishes: Escape Kinematics of the Chinook Salmon (Oncorhynchus tshawytscha)

C-starts are high acceleration swimming movements critical forpredator avoidance by fishes. Since larval fishes are particularlyvulnerable to predation, C-start behavior is likely to be especiallyimportant during early life history stages. This paper examinesthe developmental changes in C-start performance with kinematicdata on immature chinook salmon (Oncorhynchus tshawytscha) (eleuthroembryostage, sensu Balon, 1975). The scaling of C-start kinematicsof immature fishes differs from that of adults. Adult C-startdurations increase with increasing body length while C-startdurations of immature fishes decrease (e.g., adult stage 1 duration[sec] = 0.0019.length [L] [cm] $ 0.026 [R² = 0.77] [Webb, 1978];eleuthroembryos stage 1 duration [sec] = –0.026L [cm]$ 0.100 [R² = 0.81]). Distance traveled during stage 2 alsodiffers between adult and immature fishes. Adult distance traveledscales directly with length (distance [cm] = 0.38L^1.01 [cm],R² = 0.96 [Webb, 1978]) while chinook eleuthroembryo distancetraveled is positively allometric with length (distance [cm]=0.37L¹³¹ [cm], R² = 0.83). There are similarities in the developmentof C-starts and burst swimming. For example, mean velocity scalessimilarly between the two locomotor modes (For burst swimming:U_mean [cm/sec] = 8.1 ± 1.1L [cm] $ 4.89 [R² = 0.86] [Webband Corolla, 1981]. For C-start stage 2: U_mean [cm/sec] = 10.96L[cm] - 14.09 [R² = 0.70]). This study demonstrates that C-startescape performance improves during early post-hatching development.Comparisons of immature chinook salmon fast-starts with dataon larval burst swimming and on adult C-starts suggest thatchanges specific to developing fish affect the scaling of kinematicparameters.     

New Observations on the Branching of Carboniferous Ferns and Pteridosperms

Additional information is presented on the oldest known exampleof axillary branching seen in some Calamopitys from the lowermostCarboniferous of France; these putative pteridosperms are amongstthe earliest plants to be attributed to the spermatophytes.Comparative anatomical studies of coenopterid ferns from theLower and Upper Carboniferous show four categories of caulinebranching morphology: unbranched, dichotomous, lateral-monopodialand branches borne on leaves. It is suggested that leaf evolutionand thus, the axillary type of branching, was achieved earlierin the pteridosperms than in the ferns. Calamopitys, pteridosperms, coenopterid ferns, axillary branching, stem dichotomy, evolution, Carboniferous, fossil anatomy     

The Effect of Segment Length on Conductance Measurements in Lonicera fragrantissima

The effect of stem segment length on conductance measurementswas determined by repeatedly shortening the transport distancein isolated stem segments of Lonicera fragrantissima. If vesselends limit water flow, shorter segment lengths could resultin higher readings of K_h (hydraulic conductance per unit pressuregradient). The mean K_h in the successively shortened segmentsshowed no statistically significant change when shortened from20 to 2 cm, even though maximum vessel lengths ranged from 6to 14 cm. This suggests vessel ends may be less limiting towater flow than are vessel lumens. There was a statisticallysignificant drop in K_h (19%, s.e.=0·29%) when segmentswere shortened from 2 to 1 cm, regardless of the position ofthe segment within the branch. The K_h remained constant withapplied pressure in 2 cm segments while it was more variablewith applied pressure in 1 cm segments. Based on the paint-infusionmethod mean vessel lengths were from 0·6 to 1·2cm. The consistently lower K_h in 1 cm segments might be dueto an end effect in segments with a high percentage of cut openvessels. Within a branch, distal and proximal stem segmentshad lower K_h values than segments from intermediate positions,but nodes had no measurable impact on K_h. Longer segments allowfor more accurate and consistent measurements of K_h since theyintegrate localized variation in K_h while minimizing the segmentend effect. Key words: Vessel length, vessel end, hydraulic conductance, paint-infusion method, Lonicera fragrantissima     

Light Quality and Vernalization Interact in Controlling Late Flowering in Arabidopsis Ecotypes and Mutants

The late flowering ecotypes of Arabidopsis thaliana L. (Heyn.)Eifel, Pitztal and Innsbruck responded to 10 d vernalization(cold treatment) by flowering earlier with less with less thanhalf the number of leaves of non-induced plants. The vernalizationresponse was cumulative: increased numbers of days of vernalizationinduced earlier flowering up to an apparent saturation in responseafter 30 to 40 d. The ratio of red:far-red (R:FR) light alsoaffected non-vernalized time-to-flower. When grown under fluorescentplus incandescent lamps (R:FR = 1·0), time-to-flowerwas approximately half that required by plants grown under fluorescentlamps (R:FR = 5·8) at the same photon flux density andphotoperiod. Leaf production rate was unaffected by either vernalizationor light quality changes and time-to-flower and leaf numberwere highly correlated (r² = 0·973). The late flowering mutants of Landsberg erecta were grown underlighting which displayed a gradient of R:FR. Some mutants likeco, flowered at the same time in all R:FR treatment, while otherlike fca took nearly twice as long to flower, with double thenumber of leaves at R:FR ratio of 5·8 compared with theR:FR = 1 treatment. The ranking of the response from least tomost responsive was co, fe, gi, WT, fd, fwa, ft, fha, fpa, fy,fve and fca. Vernalization of these Landsberg mutants always resulted inearlier flowering, although only fca, fve, fy and fpa were significantlymore sensitive to thermoinduction than the wild type parent.There was a high correlation (r² = 0·89 between the responseto thermoinduction and to R:FR ratio. Vernalization of fca for24 d largely eliminated the R:FR time-to-flower response. Vernalizationand photoinduction similarly affect late flowering and can substitutefor each another.Copyright 1993, 1999 Academic Press Light quality, vernalization, flowering, Arabidopsis thaliana, phytochrome, thermoinduction, photoperiod, photoinduction, growth conditions, photon flux density, daylength, spectral quality, far-red light     

EASTERN MEDITERRANEAN OPISTHOBRANCHIA: RUNCINIDAE (RUNCINACEA), WITH A REVIEW OF RUNCINID CLASSIFICATION AND A DESCRIPTION OF A NEW SPECIES FROM FIJI

An account is given of the runcinaceans Runcina adriatica, R.brenkoae and R. zavodniki collected on diving expeditions inthe northern Adriatic Sea. The new data permit a clear separationof these from other runcinaceans of the north Atlantic Ocean.Several diagnostic features of the radulae and the oral cuticu-lararmature are corrected or amplified. Dental metamorphoses aredescribed for R. adriadca and R. zavodniki. The world's runcinaceanfamilies, genera and species are reviewed, and Runcina fijiensisn.sp. is described from the Pacific island of Viti Levu, Fiji (Received 14 December 1987; accepted 7 January 1988)     

Physiological Studies on Nodule Formation. The Characteristics and Inheritance of Abnormal Nodulation of Trifolium pratense by Rhizobium leguminosarum

Five to 7 per cent of plants of Trifolium repens L. and T.pratenseL. and 100 per cent of plants of T. subterraneum L. were nodulatedby Rhizobium leguminosarum but none of T. hybridum L., T. glomeratumL. or T parvifirum Ehrh. The frequency of nodulation of T. pratenseby R. leguminosarum was much increased by breeding from susceptibleplants. Such plants were not nodulated by bacteria isolatedfrom any other cross-inoculation group, but remained fully susceptibleto R. trifolii. The nodules formed by R. leguminosarum are generallyassociated with lateral roots and are ineffective.     

Hair Curling Induced in Heterologous Legumes and Monocots by Flavonoid-Treated Rhizobia

The curling of root hairs and the deformation response wereobserved when white clover was infected with homologous strainsof Rhizobium leguminosarum biovar trifolii 4S and 0403. In thecase of Rhizobium meliloti NZ and Rhizobium leguminosarum biovarviciae 128C53, however, curling was only induced when thesebacteria were pretreated with flavonoids: luteolin in the caseof R. meliloti and naringenin for R.I. viciae. The same resultswere obtained with oat, a monocotyledonous non-leguminous plant.The two flavonoids mentioned are secreted from the host plantsand induce the expression of genes for root hair curling (Hac)on Sym plasmid in homologous rhizobia, therefore, the curlingresponse in both white clover and oat appears to be correlatedwith the activation of the Hac genes. These results suggestthat a factor(s) that activates the Hac genes, such as 7,4'-dihydroxyflavonewhich is known as the factor required by R. I. trifolii, issecreted from the oat roots. (Received June 12, 1989; Accepted November 9, 1989)     

Mechanical and anatomical adaptations in terrestrial and aquatic buttercups to their respective environments

The mechanical adaptations of the stems of four species of Ranunculusto their respective environments were studied by combining tensile,bending and flow-tunnel tests, with anatomical observation. Stems of the two terrestrial species, R. acris and R. repens,had high values for rigidity, EI, because they were stiffenedby large quantities of peripherally placed lignified material.This trend is less evident in R. repens, which had a lower rigidity,though it with stood a higher breaking strain than R. acris.This may adapt R. repens to its creeping habit and help it withstandtrampling. The aquatic R. peltatus and R. fluitans, which live in stilland fast-flowing water, respectively, are both more flexibleand have higher breaking strains, of 0.1–0.15, than terrestrialplants, which may allow them to withstand sudden tugs due toflow. R. peltatus maintains the central lumen, places structuralelements away from the centre, and has a higher rigidity thanR. fluitans, which may allow it to avoid self-shading, and supportitself when the water level falls. The stem of R. fluitans shows adaptations for withstanding dragfrom fast-flowing water. The stem has a low rigidity which allowsit to minimize inertial drag forces by aligning itself parallelto the direction of the local flow. However, the rigidity (andthe second moment of area, I) does not appear to be minimized.This may allow the plant to avoid drag due to flag-like fluttering.A weak region of the stem near the base may act as a ‘mechanicalfuse’ which protects the root system by allowing seasonalgrowth to be lost. Key words: Ranunculus, mechanics, flow, anatomy     

Aspects of the Comparative Physiology of Ranunculus bulbosus L. and Ranunculus repens L.: II. Carbon Dioxide Assimilation and Distribution of Photosynthates

Detailed analysis of the interrelationships between sourcesof photosynthate production and sites of utilization in thetaxonomically closely related species Ranunculus bulbosus L.and R. repens L. showed that leaves whether present on rosette,stem, or stolon had similar levels of ¹⁴CO₂-fixation but thepattern of distribution of radiocarbon to the rest of the plantdiffered. Fruits of R. bulbosus had a lower fixation rate thanleaves but were characterized by total retention of the fixedradiocarbon. Rosette leaves of R. bulbosus supplied the youngleaves, developing apices in the rosette, roots, and corms,whereas the labelled assimilates from cauline leaves were evenlydistributed between reproductive and vegetative parts. The cormwas the major sink both at the flowering and fruiting stages.When plants were treated with ¹⁴CO₂ in the field even higherlevels of radiocarbon moved into the corm than in comparableexperiments under greenhouse conditions. The rosette leaf ofR. repens exported mainly to actively growing stolons in plantswith many stolons bearing rooted ramets although growth of astolon was also substantially supported by photosynthates producedby its own ramets. A proportion of the radiocarbon fixed byleaves of mature ramets was exported and moved in a predominantlyacropetal direction into the stolon apex, stolon axis, and youngramets of the same stolon. The stock in R. repens had a muchlower demand for assimilates than the corm in R. bulbosus. The results are consistent with the concept that R. bulbosusoperates a conservative policy involving the replacement ofthe parent in situ by a daughter from the corm, coupled withextensive fruit production. In R. repens the emphasis is onlateral spread and exploitation of substantial areas of groundby vegetative spread and replacement of the parent by daughtersmany of which may occupy sites some distance from the parent.     

Electroantennographic resolution of pulsed pheromone plumes in two species of moths with bipectinate antennae

Trains of 20-ms-duration pulses of pheromone were deliveredat rates of 1–33 Hz to antennal preparations of malesof Bombyx mori and Lymantria dispar, two moth species with bipectinateantennae. Resolution of rapidly pulsed plumes of pheromone wasnot compromised by a complex antennal morphology or by moderatechanges in wind speed (25–50 cm/s). Fourier analysis ofthe electroantennograms resolved the temporal structure of thesignal at frequencies up to 25 Hz for B. mori and up to 5 Hzfor L. dispar. The ability of these sensory structures to identifythe original (unchanged) frequency of the pulse train is particularlynoteworthy because air is slowed by about an order of magnitudeas it passes through bipectinate antennae. Although an unchangingfrequency in slowed airflow may be counterintuitive, this flowpattern, and its effects on odorant patch shape and spacing,is explained from fluid mechanical principles (i.e., the principleof continuity). An unchanging frequency suggests that as deceleratingair passes through a bipectinate antenna, the slowed patchesof odorant are stretched, thinned, and brought closer togetherby the same factor with which they are slowed.     

中国细草螟属Roxita的研究及二新种记述

对中国细草螟属Roxita Bleszynski, 1963进行了订正研究。对该属在中国分布的6个种进行了记述和特征绘图,包括２新种：福建细草螟R. fujianella sp.nov．,模式产地福建崇安,与宽带细草螟R. eurydyce近缘;云南细草螟 R. yunnanella sp.nov．,模式产地云南屏边,与双纹细草螟 R. bipunctella近缘。给出了新种与近缘种的比较及世界全部已知种检索表。     

Experimental evaluation of herbivory in the ctenophore Mnemiopsis leidyi relevant to ctenophore-zooplankton-phytoplankton interactions in Narragansett Bay, Rhode Island, USA

Herbivory of Mnemiopsis leidyi and its interactions with phytoplanktonand non-gelatinous zooplankton were examined in small-scalemicrocosm experiments. Clearance rates for M. leidyi incubatedwith phytoplankton were generally negative, but ranged up to4.5 1 ctenophore^–1 day^–1 when the large (80 µmø) diatom Ditylum brightwelli was offered as a food source.These highest ingestion rates would provide Mnemiopsis withonly 21 % of its daily carbon requirements for respiration.Mean shrinkage of M. leidyi was 8.2–51% when incubatedwith phytoplankton. Although M. leidyi neither fed activelyon phytoplankton, nor satisfied its nutritional needs on sucha diet, the chain-forming diatom Skeletonema costatum becameentangled in mucus strands and balls produced by M. leidyi inthe absence of zooplankton. Attachment onto mucus occurred atphytoplankton concentrations commonly observed in NarragansettBay and may be important in the formation of "marine snow" duringsummer M. leidyi pulses; phytoplankton sinking rate and the"package size" available to herbivores would also be affected.The experiments support our previous hypothesis based on fieldobservations in Narragansett Bay that M. leidyi indirectly regulatesphytoplankton abundance there during the summer bloom as a consequenceof predation on zooplankton. The extent to which M. leidyi influencedphytoplankton dynamics in the microcosms was dependent on therelative abundance and physiological state of the three trophiclevels. A food web diagram for M. leidyi is presented.