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1.
Mickova A Buzgo M Benada O Rampichova M Fisar Z Filova E Tesarova M Lukas D Amler E 《Biomacromolecules》2012,13(4):952-962
The broader application of liposomes in regenerative medicine is hampered by their short half-life and inefficient retention at the site of application. These disadvantages could be significantly reduced by their combination with nanofibers. We produced 2 different nanofiber-liposome systems in the present study, that is, liposomes blended within nanofibers and core/shell nanofibers with embedded liposomes. Herein, we demonstrate that blend electrospinning does not conserve intact liposomes. In contrast, coaxial electrospinning enables the incorporation of liposomes into nanofibers. We report polyvinyl alcohol-core/poly-ε-caprolactone-shell nanofibers with embedded liposomes and show that they preserve the enzymatic activity of encapsulated horseradish peroxidase. The potential of this system was also demonstrated by the enhancement of mesenchymal stem cell proliferation. In conclusion, intact liposomes incorporated into nanofibers by coaxial electrospinning are very promising as a drug delivery system. 相似文献
2.
Elisa Rasouli Wan Jeffrey Basirun Mohd Rafie Johan Majid Rezayi Majid Darroudi Kamyar Shameli Zohreh Shanavaz Omid Akbarzadeh Zahra Izadiyan 《Journal of cellular biochemistry》2019,120(4):6624-6631
In the present research, we report a greener, faster, and low-cost synthesis of gold-coated iron oxide nanoparticles (Fe3O4/Au-NPs) by different ratios (1:1, 2:1, and 3:1 molar ratio) of iron oxide and gold with natural honey (0.5% w/v) under hydrothermal conditions for 20 minutes. Honey was used as the reducing and stabilizing agent, respectively. The nanoparticles were characterized by X-ray diffraction (XRD), UV-visible spectroscopy, field emission scanning electron microscope (FESEM), energy-dispersive X-ray spectroscopy (EDXS), transmission electron microscopy (TEM), selected area electron diffraction (SAED), vibrating sample magnetometer (VSM), and fourier transformed infrared spectroscopy (FT-IR). The XRD analysis indicated the presence of Fe3O4/Au-NPs, while the TEM images showed the formation of Fe3O4/Au-NPs with diameter range between 3.49 nm and 4.11 nm. The VSM study demonstrated that the magnetic properties were decreased in the Fe3O4/Au-NPs compared with the Fe3O4-NPs. The cytotoxicity threshold of Fe3O4/Au-NPs in the WEHI164 cells was determined by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. It was demonstrated no significant toxicity in higher concentration up to 140.0 ppm which can become the main candidates for biological and biomedical applications, such as drug delivery. 相似文献
3.
The aim of this work was to produce and characterize solid lipid nanoparticles (SLN) containing levothyroxine sodium for oral administration, and to evaluate the kinetic release of these colloidal carriers. SLNs were prepared by microemulsion method. The particle size and zeta potential of levothyroxine sodium-loaded SLNs were determined to be around 153 nm,?43 mV (negatively charged), respectively by photon correlation spectroscopy. The levothyroxine entrapment efficiency was over 98 %. Shape and surface morphology were determined by TEM and SEM. They revealed fairly spherical shape of nanoparticles.SLN formulation was stable over a period of 6 months. There were no significant changes in particle size, zeta potential and polydispersity index and entrapment efficiency, indicating that the developed SLNs were fairly stable. 相似文献
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Bahareh Khodashenas Mehdi Ardjmand Azim Akbarzadeh Khiyavi 《Journal of biomolecular structure & dynamics》2020,38(15):4644-4654
AbstractCommunicated by Ramaswamy H. Sarma 相似文献
6.
With the remarkable development of nanotechnology in recent years, new drug delivery approaches based on the state-of-the-art nanotechnology have been receiving significant attention. Nanoparticles, an evolvement of nanotechnology, are increasingly considered as a potential candidate to carry therapeutic agents safely into a targeted compartment in an organ, particular tissue or cell. These particles are colloidal structures with a diameter smaller than 1,000 nm, and therefore can penetrate through diminutive capillaries into the cell's internal machinery. This innovative delivery technique might be a promising technology to meet the current challenges in drug delivery. When loaded with a gene or drug agent, nanoparticles can become nanopills, which can effectively treat problematical diseases such as cancer. This article summarizes different types of nanoparticles drug delivery systems under investigation and their prospective therapeutic applications. Also, this article presents a closer look at the advances, current challenges, and future direction of nanoparticles drug delivery systems. 相似文献
7.
A novel label-free electrochemical immunoassay based on core/shell Ag@Au nanoparticles monolayer as sensing interface has been developed for probing IgG. Several coupling techniques, such as Ag nanoparticles, Au nanoparticles, and the core/shell Ag@Au nanoparticles with L-Cysteine (Cys) cross-linking, were investigated for the determination of IgG and a very good result was obtained with the core/shell Ag@Au nanoparticles coupling. With the core/shell Ag@Au nanoparticles coupling method, the effects of the incubation time and pH on amperometric responses of the immunoassay were studied. The strong attachment of the cross-linked complex to the core/shell Ag@Au nanoparticles surface resulted in an excellent storage lifetime of 33 days. A dynamic concentration range of 2.3 to 960 ng/mL with a detection limit of 10 ng/mL was observed. Analytical results of 30 human serum samples obtained using the developing technique are in satisfactory agreement with those given by ELISA. In addition, it presents some superior advantages over the traditional sandwich format in that the analyzing performances are direct, rapid, and simple without multiple separation and labeling steps. 相似文献
8.
Weslen S. Vedakumari Periyathambi Prabu Saravana C. Babu Thotapalli P. Sastry 《Biochimica et Biophysica Acta (BBA)/General Subjects》2013
Background
Several issues have been raised emphasizing the harmful toxic effects of metal nanoparticles towards biological systems. Search of biological nanoparticles with excellent biocompatibility and bioavailability could address this problem.Methods
Fibrin nanoparticles (FNP) were prepared using a novel technique and characterized for their physico-chemical properties. In vitro studies were performed to examine cytotoxicity and cellular uptake of FNP. Innate immune response to FNP was studied by (i) estimating in vitro generation of complement split products, C3a and C4d and (ii) in vivo expression of pro-inflammatory cytokines, TNF-α, IL-1 and IL-6. In vivo biodistribution study was carried out by intravenous administration of FITC-labelled FNP in mice.Results
FNP were spherical with size ranging from 25 to 28 nm. In vitro studies proved the biocompatibility of the nanoparticles, with their distribution across the cytoplasm and nucleus of treated cells. Complement activation studies showed insignificant increase in the level of C3a when compared with positive control. RT-PCR results revealed significant upregulation of TNF-α and downregulation of IL-6 cytokines after 6 h of FNP administration. In vivo biodistribution studies showed moderate blood circulation time, with predominant distribution of nanoparticles in the liver followed by the lungs, kidney and spleen. Haematology, serum biochemistry, and histopathology analyses demonstrated that FNP were non-toxic.Conclusion
Owing to their small size, low cost, ease of preparation and excellent biocompatibility, FNP might be a promising novel material for drug delivery applications.General significance
Our results demonstrate the safe and promising use of FNP for biomedical applications. 相似文献9.
Co–B/SiO2/NH2 magnetic nanoparticles (NPs) were prepared from a silica shell-coated Co–B core using the Stöber method and amine-modification on the surface. Glucose oxidase (GOD) was covalently immobilized on the surface of Co–B/SiO2/NH2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The magnetic NPs characteristics, such as the synthesis of Co–B/SiO2/NH2 NPs, effect of pH, temperature, and concentration of buffer for enzyme immobilization, were investigated. The optimal reaction conditions for immobilization were determined to be 0.1 M of phosphate buffer solution, pH 7.0, and 5 °C. In the case of immobilized GOD without d-glucose and with 0.1 M of d-glucose for blocking, 22.98 U/g and 24.83 U/g of their original activity were retained after 7 reuses, respectively. 相似文献
10.
Rafael Ischakov Lihi Adler-Abramovich Ludmila Buzhansky Talia Shekhter Ehud Gazit 《Bioorganic & medicinal chemistry》2013,21(12):3517-3522
Peptide-based hydrogel nanoparticles represent a promising alternative to current drug delivery approaches. We have previously demonstrated that the Fmoc-FF aromatic dipeptide building block can self-assemble in aqueous solutions to form nano-scaled ordered hydrogels of remarkable mechanical rigidity. Here, we present a scalable process for the assembly of this peptide into hydrogel nanoparticles (HNPs) aimed to be utilized as potential drug delivery carriers. Fmoc-FF based HNPs were formulated via modified inverse-emulsion method using vitamin E-TPGS as an emulsion stabilizer and high speed homogenization. The formed HNPs exhibited two distinguishable populations with an average size of 21.5 ± 1.3 and 225.9 ± 0.8 nm. Gold nanoparticles were encapsulated within the hydrogel nanoparticles as contrast agents to monitor the formation of the assemblies and their ultrastructural properties. Next, we demonstrated a robust experimental procedure developed and optimized for the formulation, purification, storage and handling procedures of HNPs. Encapsulation of doxorubicin (Dox) and 5-flourouracil (5-Fu) within the HNPs matrix showed release kinetics of the drugs depending on their chemical structure, molecular weight and hydrophobicity. The results clearly indicate that Fmoc-FF based hydrogel nanoparticles have the potential to be used as encapsulation and delivery system of various drugs and bioactive molecules. 相似文献
11.
Starch is a natural, renewable biopolymer widely used in pharmaceutical industry for the controlled release of drugs and hormones. Triphala Churna (TC) a tridoshic rasayana, rich in polyphenols and vitamin C possess balancing and rejuvenating effect on three constitutional elements that govern the human life. To enhance the solubility and efficiency of the ayurvedic drug TC, attempts were made to encapsulate the TC into starch biopolymers. Characterization of Starch Encapsulated Triphala Churna (SETC) by UV–Vis spectrum, showed a sharp absorption peak at 686 nm specific for polyherbal formulation. XRD analysis illustrated that SETC is amorphous in nature. Zeta potential and dynamic light scattering analysis illustrated that the SETC were highly stable at −12 mV with an average size of 282.9 nm. SETC exhibited high drug encapsulation efficiency and fast drug release at physiological pH 7.4. Evaluation of neuroprotective effect illustrated that SETC showed excellent free radical scavenging activity, reducing power and acetylcholinesterase inhibitory activity. SETC also showed potent antibacterial activity (against Salmonella typhi and Shigella dysenteriae) and antibiofilm activity (against ATCC MRSA 33591 and clinical strain N7). Results conclude that TC on encapsulation with starch retained its antimicrobial, antibiofilm and neuroprotective activities illustrating starch as suitable drug delivery system. 相似文献
12.
Oh KS Han SK Lee HS Koo HM Kim RS Lee KE Han SS Cho SH Yuk SH 《Biomacromolecules》2006,7(8):2362-2367
Core/shell nanoparticles with lipid core, were prepared and characterized as a sustained delivery system for protein. The lipid core is composed of protein-loaded lecithin and the polymeric shell is composed of Pluronics (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer, F-127). Based on the preparation method in the previous report by us, the freeze-drying of protein-loaded lecithin was performed in the F-127 aqueous solution containing trehalose used as a cryoprotectant to form stabilized core/shell nanoparticles. Cryo-TEM (transmittance electron microscopy) and a particle size analyzer were used to observe the formation of stabilized core/shell nanoparticles. For the application of core/shell nanoparticles as a protein drug carrier, lysozyme and vascular endothelial growth factor (VEGF) were loaded into the core/shell nanoparticles by electrostatic interaction, and the drug release pattern was observed by manipulating the polymeric shell. 相似文献
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Sorbi C Bergamin M Bosi S Dinon F Aroulmoji V Khan R Murano E Norbedo S 《Carbohydrate research》2009,344(1):91-2058
Selective halogenation of hyaluronan and partial halogen substitution by methotrexate led to 6-chloro-6-deoxy-6-O-methotrexylhyaluronan, a potential antitumor drug. The remaining halogen could be further substituted by a second organic carboxylate, leading to mixed esters. 6-O-Acetyl-6-O-methotrexylhyaluronan and 6-O-butyryl-6-O-methotrexylhyaluronan were thus synthesized and characterized by NMR spectroscopy. 相似文献
15.
M. A. Sanzhakov V. N. Prozorovskyi O. M. Ipatova E. G. Tikhonova N. V. Medvedeva T. I. Torkhovskaya 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2014,8(2):177-180
Low bioavailability of rifampicin, one of the main antituberculous agents, stimulates searches of its new optimized formulations. The present study has shown a possibility of rifampicin incorporation into nanoparticles from plant phosphatidylcholine (diameter of 20–30 nm). Addition of sodium oleate to the phospholipid system caused a 2-fold increase in the percent of rifampicin incorporation. The maximal concentration of rifampicin assayed in plasma samples by LC/MS was observed 1 h after oral administration to rats (6 mg/kg) and represented 0.5 and 4.2 μg/mL for free rifampicin and rifampicin incorporated in the phospholipids-oleate nanoparticles, respectively. These levels were maintained for more than 2 h of the experiment. High rifampicin bioavailability in the oleate containing phospholipid nanosystem suggests its prospects for practical use. 相似文献
16.
The purpose of the present research was to produce a quick/slow biphasic delivery system for ibuprofen. A dual-component tablet
made of a sustained release tableted core and an immediate release tableted coat was prepared by direct compression. Both
the core and the coat contained a model drug (ibuprofen). The sustained release effect was achieved with a polymer (hydroxypropyl
methylcellulose [HPMC] or ethylcellulose) to modulate the release of the drug. The in vitro drug release profile from these
tablets showed the desired biphasic release behavior: the ibuprofen contained in the fast releasing component was dissolved
within 2 minutes, whereas the drug in the core tablet was released at different times (⊂16 or >24 hours), depending on the
composition of the matrix tablet. Based on the release kinetic parameters calculated, it can be concluded that the HPMC core
was suitable for providing a constant and controlled release (zero order) for a long period of time.
Published: September 21, 2007 相似文献
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Tuftsin-modified alginate nanoparticles as a noncondensing macrophage-targeted DNA delivery system 总被引:1,自引:0,他引:1
The main objective of this study was to evaluate macrophage-targeted alginate nanoparticles as a noncondensing gene delivery system for potential anti-inflammatory therapy. An external gelation method was employed to form plasmid DNA-encapsulated alginate nanoparticles. The nanoparticle surface was modified with a peptide sequence containing tuftsin (TKPR), and transfection efficiency was determined in J774A.1 macrophages. The effect of transfected mIL-10 in blocking expression of tumor necrosis factor-alpha (TNF-α) was evaluated in lipopolysaccharide (LPS)-stimulated cells. Scrambled peptide- and tuftsin-modified cross-linked alginate nanoparticles efficiently encapsulated plasmid DNA and protected against DNase I degradation. The transgene expression efficiencies, measured using GFP and mIL-10 expressing plasmid DNA, were highest with tuftsin-modified nanoparticles. Levels of TNF-α were significantly lower (p < 0.0001) in LPS-stimulated cells that were transfected with mIL-10 using alginate nanoparticles. The results of the study show that noncondensing alginate nanoparticles can efficiently deliver plasmid DNA, leading to sustained in vitro gene expression in macrophages. 相似文献
19.
Saboktakin MR Tabatabaie RM Maharramov A Ramazanov MA 《International journal of biological macromolecules》2011,48(3):381-385
The purpose of this study was to examine chitosan (CS)-carboxymethyl starch (CMS) nanoparticles as drug delivery system to the colon. The 5-aminosalicylic acid (5-ASA) was chosen as model drug molecule. CS-CMS nanoparticles were formulated by a complex coacervation process under mild conditions. The influence of process variables, including the two ionic polymers, on particle size, and nanoparticles entrapment of 5-ASA was studied. In vitro release of 5-ASA was also evaluated, and the integrity of 5-ASA in the release fraction was assessed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The release of 5-ASA from nanoparticle was based on the ion-exchange mechanism. The CS-CMS nanoparticles developed based on the modulation of ratio show promise as a system for controlled delivery of drug to the colon. 相似文献
20.
Shichao Wu Xiangrui Yang Fanghong Luo Ting Wu Peilan Xu Mingyuan Zou Jianghua Yan 《Journal of nanobiotechnology》2018,16(1):90