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1.
In a taxonomic study on the ascomycetous yeasts isolated from plant materials collected in tropical forests in Yunnan and Hainan Provinces, southern China, four strains isolated from tree sap (YJ2E(T)) and flowers (YF9E(T), YWZH3C(T) and YYF2A(T)) were revealed to represent four undescribed yeast species. Molecular phylogenetic analysis based on the large subunit (26S) rRNA gene D1/D2 domain sequences showed that strain YJ2E(T) was located in a clade together with Candida haemulonii and C. pseudohaemulonii. Strain YF9E(T) was most closely related to C. azyma and strain YWZH3C(T) to C. sorbophila and C. spandovensis. Strain YYF2A(T) was clustered in a clade containing small-spored Metschnikowia species and related anamorphic Candida species. The new strains differed from their closely related described species by more than 10% mismatches in the D1/D2 domain. No sexual states were observed for the four strains on various sporulation media. The new species are therefore assigned to the genus Candida and described as Candida alocasiicola sp. nov. (type strain, YF9E(T) = AS 2.3484(T) = CBS 10702(T)), Candida hainanensis sp. nov. (type strain, YYF2A(T) = AS 2.3478(T) = CBS 10696(T)), Candida heveicola sp. nov. (type strain, YJ2E(T) = AS 2.3483(T) = CBS 10701(T)) and Candida musiphila sp. nov. (type strain, YWZH3C(T) = AS 2.3479(T) = CBS 10697(T)). 相似文献
2.
Entyloma species causing white smut disease of Cosmos species have been studied. Two species can be recognized, Entyloma holwayi on C. caudatus and C. sulphureus from North America, and E. cosmi sp. nov. on C. bipinnatus from Japan and Europe. 相似文献
3.
Murugesan Dhandapani Seung-Beom Hong Channa Reddy Aswath Doo Hwan Kim 《In vitro cellular & developmental biology. Plant》2008,44(1):8-13
We have optimized conditions for efficient regeneration of the vegetatively propagated zoysia grass (Zoysia matrella L. Merr) cultivar “Konhee”. Two explants, young inflorescences, and stem nodes, were used and they displayed different responses
to combinations and concentrations of plant growth regulators in callusing, embryogenic callus formation, and regeneration.
The highest callus initiation rate from young inflorescences was obtained on medium supplemented with 4.5 to 9.0 μM 2,4-dicholorophenoxy
acetic acid (2,4-D) and 0.44 μM 6-benzyl amino purine (BA). When the BA concentration was lowered to 0.044 μM, the highest
percent embryogenic callus induction from young inflorescences was achieved. The highest callus initiation rate from stem
nodes was obtained, when young inflorescences were cultured on MS medium supplemented with 4.5 to 9.0 μM 2,4-D, 0.44 μM BA,
and 0.037 μM abscisic acid (ABA). But embryogenic callus formation from the stem node was highest in the presence of 4.5 to
9.0 μM 2,4-D, 0.044 μM BA, and 0.037 μM ABA. Addition of ABA significantly increased embryogenic callus formation from stem
nodes, but not from young inflorescences. Regeneration percentage was variable in response to BA level, and inclusion of α-naphthalene
acetic acid (NAA) and gibberellic acid (GA3) further increased the regeneration percentage. The highest regeneration percentages obtained from the young inflorescences
and stem nodes were 82% and 67%, respectively. This is the first report showing that plants can be regenerated from young
inflorescences and stem nodes of vegetatively propagated zoysia grass. 相似文献
4.
Trimmatostroma abietis from the needles of conifers and from stone, mostly in Germany, is described. On the natural substrate it is characterized by stromatic conidiomata. Conidia are mostly two-celled, are arranged in long chains, and originate by intercalary dilatation of fertile hyphae. The wide morphological plasticity of the fungus and its physiological profile are described. Its occurrence as a saprophyte and possibly opportunistic pathogen on plants and as an invader of environments with low water activity, particularly stone surfaces, are discussed. Relationships with other taxa are outlined. 相似文献
5.
Kurtzman CP 《Antonie van Leeuwenhoek》2007,92(2):221-231
Three new species of Candida and a new species of Trigonopsis are described based on their recognition from phylogenetic analysis of gene sequences from large subunit ribosomal RNA, ITS1/ITS2
rRNA, mitochondrial small subunit rRNA and cytochrome oxidase II. Candida
infanticola sp. nov. (type strain NRRL Y-17858, CBS 7922) was isolated from the ear of an infant in Germany and is closely related to
Candida sorbophila. Candida polysorbophila sp. nov. (type strain NRRL Y-27161, CBS 7317) is a member of the Zygoascus clade and was isolated in South Africa as a contaminant from an emulsion of white oil and polysorbate. Candida transvaalensis sp. nov. (type strain NRRL Y-27140, CBS 6663) was obtained from forest litter, the Transvaal, South Africa, and forms an
isolated clade with Candida santjacobensis. Trigonopsis californica sp. nov. (type strain NRRL Y-27307, CBS 10351) represents a contaminant from wine in California, and forms a well-supported
clade with Trigonopsis cantarellii, Trigonopsis variabilis and Trigonopsis vinaria. 相似文献
6.
Harald Riedl 《Plant Systematics and Evolution》1981,138(3-4):283-286
Cynoglossopsis somaliensis
H. Riedl, sp. nov., is described as a new species of the hitherto monotypic genusCynoglossopsis
Brand which is closest related toCynoglossum L. but has to be included inBoraginoideae-Eritrichieae from the way the nutlets are attached to the gynobasis. 相似文献
7.
Four novel yeast species are described, two from decaying mushrooms, viz. Candida cretensis and Candida vadensis, and two from rotten wood, viz. Blastobotrys robertii and Candida scorzettiae. Accession numbers for the CBS and ARS Culture Collections, and GenBank accession numbers for the D1/D2 domains of the large
subunit of ribosomal DNA are: B. robertii CBS 10106T, NRRL Y-27775, DQ839395; C. cretensis CBS 9453T, NRRL Y-27777, AY4998861 and DQ839393; C. scorzettiae CBS 10107T, NRRL Y-27665, DQ839394; C. vadensis CBS 9454T, NRRL Y-27778, AY498863 and DQ839396. The GenBank accession number for the ITS region of C. cretensis is AY498862 and that for C. vadensis is AY498864. C. cretensis was the only species of the four that displayed fermentative activity. All four type strains grew on n-hexadecane. C. scorzettiae is the only one of the new species that assimilates some phenolic compounds, viz. 3-hydroxy derivatives of benzoic, phenylacetic
and cinnamic acids, but not the corresponding 4-hydroxy acids. This is indicative of an operative gentisate pathway. 相似文献
8.
Lengyel K Lang E Fodor A Szállás E Schumann P Stackebrandt E 《Systematic and applied microbiology》2005,28(2):115-122
The taxonomic affiliation was determined for four Xenorhabdus strains isolated from four Steinernema hosts from different countries. As compared to the five validly described Xenorhabdus species, i.e., X. nematophila, X. japonica, X. beddingii, X. bovienii and X. poinarii, these isolates represented novel species on the basis of 16S rRNA gene sequences and riboprint patterns, as well as by physiological and metabolic properties. They were named Xenorhabdus budapestensis sp. nov., type strain DSM 16342T, isolated from Steinernema bicornutum; Xenorhabdus ehlersii sp. nov., type strain DSM 16337T, isolated from Steinernema serratum; Xenorhabdus innexi sp. nov., type strain DSM 16336T isolated from Steinernema scapterisci; and Xenorhabdus szentirmaii sp. nov., type strain DSM 16338T, isolated from Steinernema rarum. 相似文献
9.
Transgenic zoysiagrass (Zoysia japonica) plants obtained by Agrobacterium-mediated transformation 总被引:1,自引:0,他引:1
Zoysiagrass (Zoysia japonica Steud.) is an important turfgrass that spreads by stolons and rhizomes. By exploring the potential of direct shoot formation from stolons, we developed a straightforward and efficient transformation protocol without callus induction and propagation. Sterilized stolon nodes were infected and co-cultivated with Agrobacterium tumefaciens harboring pCAMBIA vectors. Hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin was used as the selection agent. Both green and albino shoots were directly regenerated from the infected stolon nodes 4–5 weeks after hygromycin selection. Greenhouse-grown plants were obtained 10–12 weeks after Agrobacterium-mediated transformation. Based on the number of transgenic plants obtained and the number of stolon nodes infected, a transformation frequency of 6.8% was achieved. Stable integration of the transgenes in the plant genome was demonstrated by PCR and Southern blot hybridization analyses. Expression of the transgenes was confirmed by RT-PCR analysis and GUS staining. The new transformation system opens up new opportunities for the functional characterization of genes and promoters and the development of novel germplasm in zoysiagrass. 相似文献
10.
Diseases caused in wheat by Bipolaris sorokiniana and Drechslera tritici-repentis have led to considerable yield and production losses. In wheat seeds another isolate has recently been identified, resembling Bipolaris bicolor. The objective of the present trial was to differentiate and identify isolates of these fungi based on electrophoretic analyses and morphology. Esterase electrophoresis enabled the differentiation between Drechslera sp. and Bipolaris sp. isolates. In relation to morphology, conidia from D. tritici-repentis isolates were significantly longer than the isolates of B. sorokiniana. Bipolaris bicolor isolates, on the other hand, presented wider conidia than those of D. tritici-repentis and B. sorokiniana. 相似文献
11.
A new Conchostracan species, Eocyzicus spinifer from Iraq is described. A table is given to distinguish the known species of the genus Eocyzicus. 相似文献
12.
O. R. Kotsyurbenko M. V. Simankova A. N. Nozhevnikova T. N. Zhilina N. P. Bolotina A. M. Lysenko G. A. Osipov 《Archives of microbiology》1995,163(1):29-34
Three strains of new acetogenic bacteria were isolated from several low temperature environments. Cells were gram-positive, oval-shaped flagellated rods. The organisms fermented H2/CO2, CO, formate, lactate, and several sugars to acetate. Strains Z-4391 and Z-4092 grew in the temperature range from 1 to 30°C with an optimum at 20°C; strain Z-4290 grew in the range from 1 to 35°C with an optimum at 30°C. The DNA G+C content of strains Z-4391, Z-4092, and Z-4290 was 42.1, 41.7, and 45.8 mol% respectively. 相似文献
13.
Manfio GP Atalan E Zakrzewska-Czerwinska J Mordarski M Rodríguez C Collins MD Goodfellow M 《Antonie van Leeuwenhoek》2003,83(3):245-255
The taxonomic positions of soil isolates known as Streptomyces groups A, B and C were clarified. Comparative 16S rDNA sequence studies indicated that representatives of all three taxa
formed distinct phyletic lines within the Streptomyces tree though the group A strains were shown to be related to Streptomyces griseus and associated validly described species. The taxonomic integrity of all three groups was highlighted by DNA:DNA relatedness
and ribotype data though the group A strains encompassed a higher degree of genetic variation than the group B and C strains.
In light of these and earlier phenotypic data it is proposed that Streptomyces groups A, B and C be given species status as Streptomyces sanglieri sp. nov., Streptomyces aureus sp. nov. and Streptomyces laceyi sp. nov., respectively.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
14.
Takeo Oguchi 《Mycoscience》2001,42(2):217-221
A new species ofAciculosporium, A. sasicola (anam.Albomyces sasicola), is described and illustrated. The fungus differs fromAciculosporium take by its small asci and ascospores, though witches' broom and signs probably caused by the former onSasa senanensis are similar to those caused by the latter on bamboos. 相似文献
15.
Daphnia (C.) hispanica n. sp. has thoracic limbs of A D. (C.) atkinsoni type and is very similar toD. chevreuxi Richard, 1896. With this speciesD. (C.) hispanica shares the presence of a soft accessory seta on the outer side of the trunk of the first thoracic limb, apparently unique among the genusDaphnia. Although other morphological characteristics are very similar in this two species, a detailed comparison reveals that they can easily be separated as different species. The most useful features in differentiating both species are:D. (C.) hispanica has a dorsal crest in the head, its rostrum is longer, its accesory seta of the first thoracic limb is more developed, the juveniles are not helmeted and its male postabdomen lacks individual denticles on the anal and preanal margin.The new species is, up to now, restricted to Spain and inhabits the temporary and low mineralized waters of the steppe regions. 相似文献
16.
Spirobolbolaimus bathyalis gen. nov., sp. nov. is described from the continental slope off Calvi (Mediterranean). The new genus Spirobolbolaimus belongs to the Microlaimidae Micoletzky, 1922 and is closely related to the genus Bolbolaimus Cobb, 1920. It can be differentiated from Bolbolaimus by its multispiral amphideal fovea and by its six rows of postamphideal setae. 相似文献
17.
From approximately 200 basidiomycetous yeast isolates forming orange or orange-red colonies isolated from senescent leaves collected in different regions of China, 29 representative strains varying in their geographic distribution and ballistoconidium forming ability were selected for further phenotypic and molecular taxonomic studies. Sequence analysis of the large subunit (26S) rDNA D1/D2 domain and the internal transcribed spacer (ITS) region including 5.8S rRNA from the strains resulted in the recognition of seven Dioszegia species, including four described species, namely D. aurantiaca, D. fristingensis, D. hungarica and D. zsoltii var. zsoltii and D. zsoltii var. yunnanensis, and three undescribed species. The three new species are described as Dioszegia athyri sp. nov. (type strain: CB 159(T) = AS 2.2559(T) = CBS 10119(T)), Dioszegia butyracea sp. nov. (type strain: CB 261(T) = AS 2.2600(T) = CBS 10122(T)) and Dioszegia xingshanensis sp. nov. (type strain: HB 1.4(T) = AS 2.2481(T) = CBS 10120(T)) in the present study. 相似文献
18.
Itoh T Yamaguchi T Zhou P Takashina T 《Extremophiles : life under extreme conditions》2005,9(2):111-116
Three novel isolates of haloalkaliphilic archaea, strains IHC-005T, IHC-010, and N-1311T, from soda lakes in Inner Mongolia, China, were characterized to elucidate their taxonomic positions. The three strains were aerobic, Gram-negative chemoorganotrophs growing optimally at 37–45°C, pH 9.0–9.5, and 15–20% NaCl. Cells of strains IHC-005T/IHC-010 were motile rods, while those of strain N-1311T were non-motile pleomorphic flats or cocci. The three strains contained diphytanyl and phytanyl-sesterterpanyl diether derivatives of phosphatidylglycerol and phosphatidylglycerophosphate methyl ester. No glycolipids were detected. On phylogenetic analysis of 16S rRNA gene sequences, they formed an independent cluster in the Natro group of the family Halobacteriaceae. Comparison of their morphological, physiological, and biochemical properties, DNA G+C content and 16S rRNA gene sequences, and DNA-DNA hybridization study support the view that strains IHC-005T/IHC-010 and strain N-1311T represent separate species. Therefore, we propose Natronolimnobius baerhuensis gen. nov., sp. nov. for strains IHC-005T (=CGMCC 1.3597T =JCM 12253T)/IHC-010 (=CGMCC 1.3598=JCM 12254) and Natronolimnobius innermongolicus sp. nov. for N-1311T (=CGMCC 1.2124T =JCM 12255T). 相似文献
19.
Alternaria jesenskae sp. nov. recovered from seeds of a shrubby perennial plant Fumana procumbens (Cistaceae) in Slovakia is described and illustrated. The new taxon can be clearly separated from the other related large-spored and filament-beaked Alternaria species based on sequences of the ITS1, 5.8S and ITS2 region as well as by its distinctive morphology. Even though the molecular data have shown close relatedness with A. multirostrata, the new species is morphologically most similar to A. tomatophila distinguished primarily by the pronounced colony pigmentation, conidial septation and beak branching. 相似文献
20.
Aecidium dispori forms spermogonium and aecium onDisporum sessile andD. smilacinum, which are distributed in East Asia. TheAecidium species is found to be an aecial anamorph of aPuccinia fungus, with its uredinial-telial stage being formed onCarex conica, C. dolichostachya subsp.multifolia, C. pisiformis subsp.alterniflora andC. rugata. Urediniospores of this fungus are large, colorless, thick-walled with 4–5 equatorial germ pores. The morphological characteristics
of urediniospores and the spermogonial-aecial host do not fit to any set of circumscribing characters of previously described
species. We consider the fungus to be a new species and propose a new name,Puccinia albispora, for the fungus. 相似文献