共查询到20条相似文献,搜索用时 15 毫秒
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Patrick C. Gallagher Teri L. Lear Linda D. Coogle Ernest Bailey 《Mammalian genome》1999,10(2):140-144
BLAST searches of 61 equine microsatellite sequences revealed two related families of retroposons. The first family included
seven markers, all of which showed significant homology to the Equine Repetitive Element-1 (ERE-1) Short Interspersed Nucleotide
Element (SINE) sequence. Length of homology ranged from 76 to 171 bases with identities to the ERE-1 consensus sequence ranging
from 71% to 83%. The second family referred to as Equine Repetitive Element-2 (ERE-2) has a consensus sequence that showed
homology to ERE-1 over approximately 60 bases. These 60 bases comprised subunit I. Sequence comparisons for the two retroposons
led to the identification of a subunit II, subunit III, as well as the tRNAser subunit. The subunit structure of ERE-1 was tRNAser-I-II. By contrast, the subunit structure of ERE-2 was I-III-III. The
nine markers related to ERE-2 showed homology lengths ranging from 84 to 163 bases with identities ranging from 75% to 99%.
In addition to being present in microsatellites, ERE-2 appeared in three separate equine genes. It occurred in an intron of
DNA-PK, in an untranslated region as well as in the promoter of PGHS, and in the coding region of PAM. The amino acids corresponding
to the ERE-2 sequence in PAM were not present in the human or mouse PAM homologs. These amino acids associated with the ERE-2
sequence were present on the cytosolic side of the transmembrane domain of the PAM enzyme.
Microsatellite markers in the ERE-1 and ERE-2 families were found throughout the genus equus and also for rhinoceros, indicating
that the appearance of both retroposons predates the divergence of equids from the other perissodactyls. The markers did not
amplify in human or bovine DNA. This indicated that ERE-1 and ERE-2 are, at least, perissodactyl specific.
Received: 20 July 1998 / Accepted: 29 September 1998 相似文献
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Lee JM Nahm SH Kim YM Kim BD 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(4):619-627
Microsatellites or simple sequence repeats are highly variable DNA sequences that can be used as informative markers for the genetic analysis of plants and animals. For the development of microsatellite markers in Capsicum, microsatellites were isolated from two small-insert genomic libraries and the GenBank database. Using five types of oligonucleotides, (AT)15, (GA)15, (GT)15, (ATT)10 and (TTG)10, as probes, positive clones were isolated from the genomic libraries, and sequenced. Out of 130 positive clones, 77 clones showed microsatellite motifs, out of which 40 reliable microsatellite markers were developed. (GA)
n
and (GT)
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sequences were found to occur most frequently in the pepper genome, followed by (TTG)
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and (AT)
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. Additional 36 microsatellite primers were also developed from GenBank and other published data. To measure the information content of these markers, the polymorphism information contents (PICs) were calculated. Capsicum microsatellite markers from the genomic libraries have shown a high level of PIC value, 0.76, twice the value for markers from GenBank data. Forty six microsatellite loci were placed on the SNU-RFLP linkage map, which had been derived from the interspecific cross between Capsicum annuum TF68 and Capsicum chinense Habanero. The current SNU2 pepper map with 333 markers in 15 linkage groups contains 46 SSR and 287 RFLP markers covering 1,761.5 cM with an average distance of 5.3 cM between markers.Communicated by J. Dvorak 相似文献
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Tozaki T Kakoi H Mashima S Hirota K Hasegawa T Ishida N Miura N Tomita M 《Animal genetics》2000,31(3):234-236
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