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Cao Yujin Zhang Haibo Liu Hui Liu Wei Zhang Rubing Xian Mo Liu Huizhou 《Applied microbiology and biotechnology》2018,102(4):1535-1544
Applied Microbiology and Biotechnology - Sabinene is an important naturally occurring bicyclic monoterpene which can be used as flavorings, perfume additives, fine chemicals, and advanced biofuels.... 相似文献
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Biosynthesis and biotechnological production of flavanones: current state and perspectives 总被引:1,自引:0,他引:1
Polyphenols produced in a wide variety of flowering and fruit-bearing plants have the potential to be valuable fine chemicals
for the treatment of an assortment of human maladies. One of the major constituents within this chemical class are flavonoids,
among which flavanones, as the precursor to all flavonoid structures, are the most prevalent. We review the current status
of flavanone production technology using microorganisms, with focus on heterologous protein expression. Such processes appear
as attractive production alternatives for commercial synthesis of these high-value chemicals as traditional chemical, and
plant cell cultures have significant drawbacks. Other issues of importance, including fermentation configurations and economics,
are also considered. 相似文献
4.
Min-Ji Kim Bong-Gyu Kim Joong-Hoon Ahn 《Applied microbiology and biotechnology》2013,97(16):7195-7204
Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin biosynthesis pathway in E. coli. First, the shikimic acid pathway, which leads to the biosynthesis of tyrosine, was engineered in E. coli to increase the amount of available tyrosine. Second, several genes for the biosynthesis of ponciretin and sakuranetin such as tyrosine ammonia lyase (TAL), 4-coumaroyl CoA ligase (4CL), chalcone synthase (CHS), and O-methyltransferase (OMT) were overexpressed. In order to increase the supply the Coenzyme A (CoA), one gene (icdA, isocitrate dehydrogenase) was deleted. Using these strategies, we synthesized ponciretin and sakuranetin from glucose in E. coli at the concentration of 42.5 mg/L and 40.1 mg/L, respectively. 相似文献
5.
Hyung-Moo Jung Marimuthu Jeya Sang-Yong Kim Hee-Jung Moon Raushan Kumar Singh Ye-Wang Zhang Jung-Kul Lee 《Applied microbiology and biotechnology》2009,84(3):417-428
The glycopeptide teicoplanin isolated from the fermentation broth of Actinoplanes teichomyceticus is used to treat serious Gram-positive bacterial infections that are resistant to other antibiotics, e.g. β-lactams. The long time frame and progressively broader clinical use of teicoplanin has eventually led to the emergence and
spreading of resistance in enterococci and staphylococci towards the antibiotics. Given the structural complexity of the natural
product, only fermentative routes are available for bulk production of teicoplanin even though the total synthesis of the
antibiotic has been accomplished. Because the low productivity (0.1–3.1 g/L) is a limitation to the commercial production
of teicoplanin, substantial effort has been devoted to the strain improvement and process development for enhancing the productivity.
This review summarizes the current state of the action mechanism, antibacterial activity, resistance mechanism, biotechnological
production, and application of teicoplanin.
Hyung-Moo Jung and Marimuthu Jeya equally contributed to this work. 相似文献
6.
Biogas production: current state and perspectives 总被引:16,自引:0,他引:16
Peter Weiland 《Applied microbiology and biotechnology》2010,85(4):849-860
Anaerobic digestion of energy crops, residues, and wastes is of increasing interest in order to reduce the greenhouse gas
emissions and to facilitate a sustainable development of energy supply. Production of biogas provides a versatile carrier
of renewable energy, as methane can be used for replacement of fossil fuels in both heat and power generation and as a vehicle
fuel. For biogas production, various process types are applied which can be classified in wet and dry fermentation systems.
Most often applied are wet digester systems using vertical stirred tank digester with different stirrer types dependent on
the origin of the feedstock. Biogas is mainly utilized in engine-based combined heat and power plants, whereas microgas turbines
and fuel cells are expensive alternatives which need further development work for reducing the costs and increasing their
reliability. Gas upgrading and utilization as renewable vehicle fuel or injection into the natural gas grid is of increasing
interest because the gas can be used in a more efficient way. The digestate from anaerobic fermentation is a valuable fertilizer
due to the increased availability of nitrogen and the better short-term fertilization effect. Anaerobic treatment minimizes
the survival of pathogens which is important for using the digested residue as fertilizer. This paper reviews the current
state and perspectives of biogas production, including the biochemical parameters and feedstocks which influence the efficiency
and reliability of the microbial conversion and gas yield. 相似文献
7.
Hosakatte Niranjana Murthy Yun-Soo Kim Milen I. Georgiev Kee-Yoeup Paek 《Applied microbiology and biotechnology》2014,98(17):7319-7329
Eleutherosides, the phenylpropanoid and lignan glycosides, are the active ingredients accumulated in the roots and stems of Eleutherococcus species and in Eleutherococcus senticosus in particular. Syringin (=eleutheroside B) and (?) syringaresinol-di-O-β-d-glucoside (=eleutheroside E) appear as the most important bioactive compounds which are used as adaptogens, besides their abundant antidiabetic and anticancer properties. As the availability of “Eleuthero” is becoming increasingly limited because of its scanty natural distribution, the production of these compounds by biotechnological means has become an attractive alternative. In E. senticosus and other closely related species, Eleutherococcus sessiliflorus, Eleutherococcus chiisanensis, and Eleutherococcus koreanum, organogenic cultures have been induced for the production of eleutherosides. Bioreactor cultures have been established and various parameters, which influence on the accumulation of biomass and secondary metabolites, have been thoroughly investigated. Pilot-scale cultures have also been accomplished for the large-scale production of somatic embryos containing abundant amounts of eleutherosides. This review describes the biotechnological approaches and challenges for the production of eleutherosides. 相似文献
8.
Toru Matsui Yasumasa Dekishima Makoto Ueda 《Applied microbiology and biotechnology》2014,98(18):7699-7706
Chiral organic sulfoxides (COSs) are important compounds that act as chiral auxiliaries in numerous asymmetric reactions and as intermediates in chiral drug synthesis. In addition to their optical resolution, stereoselective oxidation of sulfides can be used for COS production. This reaction is facilitated by oxygenases and peroxidases from various microbial resources. To meet the current demand for esomeprazole, a proton pump inhibitor used in the treatment of gastric-acid-related disorders, and the (S)-isomer of an organic sulfoxide compound, omeprazole, a successful biotechnological production method using a Baeyer-Villiger monooxygenase (BVMO), was developed. In this review, we summarize the recent advancements in COS production using biocatalysts, including enzyme identification, protein engineering, and process development. 相似文献
9.
Current state and perspectives in hydrogen production by Escherichia coli: roles of hydrogenases in glucose or glycerol metabolism 总被引:1,自引:0,他引:1
Maeda Toshinari Tran Kien Trung Yamasaki Ryota Wood Thomas K. 《Applied microbiology and biotechnology》2018,102(5):2041-2050
Applied Microbiology and Biotechnology - Escherichia coli has been a robust host strain for much biological research, in particular, research in metabolic engineering, protein engineering, and... 相似文献
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N-Acetyl-d-neuraminic acid (Neu5Ac) and its derivates are a very important group of biomolecules because these sugars occupy the terminal positions in numerous macromolecules, such as the glycans of glycoproteins, and are involved in many biological and pathological phenomena. The synthesis and applications of Neu5Ac are attracting much interest due to the potential applications of this compound in the pharmaceutical industry, such as in the synthesis of the anti-flu drug zanamivir. In this review article, we discuss existing knowledge on the biotechnological production and applications of Neu5Ac and also propose some guidelines for future studies. 相似文献
11.
He Wang Ruijin Yang Xiao Hua Wei Zhao Wenbin Zhang 《Applied microbiology and biotechnology》2013,97(14):6167-6180
Lactulose, a synthetic ketose disaccharide, has been widely used in food and pharmaceutical industries as prebiotic food additives and drugs against constipation and hepatic encephalopathy. Lactulose has, so far, been produced chemically using lactose on a commercial scale. The key problems associated with such chemical process are the cost of removal of the catalyst and colored by-products and the product safety. Enzymatic production of lactulose is safe, environment-friendly, and simpler in comparison to the chemical method. As a promising alternative to the chemical method, enzymatic conversion of lactose into lactulose by β-galactosidase or cellobiose 2-epimerase has recently gained a great deal of attention. This could be considered as a possible route for whey surplus because lactose is the major component of cheese whey. Herein, we summarize recent advances on the enzymatic synthesis of lactulose with emphasis on the selectivity of biocatalysts and their catalytic efficiency in free and immobilized forms. The production of 1-lactulose by enzymatic bioconversion of lactose has also been discussed. Furthermore, future research needs of β-galactosidase and cellobiose 2-epimerase for the enzymatic synthesis of lactulose and 1-lactulose are reviewed. 相似文献
12.
Salvador Peirú Eduardo Rodríguez Hugo G. Menzella John R. Carney Hugo Gramajo 《Microbial biotechnology》2008,1(6):476-486
Significant achievements in polyketide gene expression have made Escherichia coli one of the most promising hosts for the heterologous production of pharmacologically important polyketides. However, attempts to produce glycosylated polyketides, by the expression of heterologous sugar pathways, have been hampered until now by the low levels of glycosylated compounds produced by the recombinant hosts. By carrying out metabolic engineering of three endogenous pathways that lead to the synthesis of TDP sugars in E. coli, we have greatly improved the intracellular levels of the common deoxysugar intermediate TDP‐4‐keto‐6‐deoxyglucose resulting in increased production of the heterologous sugars TDP‐L‐mycarose and TDP‐d ‐desosamine, both components of medically important polyketides. Bioconversion experiments carried out by feeding 6‐deoxyerythronolide B (6‐dEB) or 3‐α‐mycarosylerythronolide B (MEB) demonstrated that the genetically modified E. coli B strain was able to produce 60‐ and 25‐fold more erythromycin D (EryD) than the original strain K207‐3, respectively. Moreover, the additional knockout of the multidrug efflux pump AcrAB further improved the ability of the engineered strain to produce these glycosylated compounds. These results open the possibility of using E. coli as a generic host for the industrial scale production of glycosylated polyketides, and to combine the polyketide and deoxysugar combinatorial approaches with suitable glycosyltransferases to yield massive libraries of novel compounds with variations in both the aglycone and the tailoring sugars. 相似文献
13.
Outdoor cultivation of microalgae for carotenoid production: current state and perspectives 总被引:1,自引:0,他引:1
Del Campo JA García-González M Guerrero MG 《Applied microbiology and biotechnology》2007,74(6):1163-1174
Microalgae are a major natural source for a vast array of valuable compounds, including a diversity of pigments, for which
these photosynthetic microorganisms represent an almost exclusive biological resource. Yellow, orange, and red carotenoids
have an industrial use in food products and cosmetics as vitamin supplements and health food products and as feed additives
for poultry, livestock, fish, and crustaceans. The growing worldwide market value of carotenoids is projected to reach over
US$1,000 million by the end of the decade. The nutraceutical boom has also integrated carotenoids mainly on the claim of their
proven antioxidant properties. Recently established benefits in human health open new uses for some carotenoids, especially
lutein, an effective agent for the prevention and treatment of a variety of degenerative diseases. Consumers’ demand for natural
products favors development of pigments from biological sources, thus increasing opportunities for microalgae. The biotechnology
of microalgae has gained considerable progress and relevance in recent decades, with carotenoid production representing one
of its most successful domains. In this paper, we review the most relevant features of microalgal biotechnology related to
the production of different carotenoids outdoors, with a main focus on β-carotene from Dunaliella, astaxanthin from Haematococcus, and lutein from chlorophycean strains. We compare the current state of the corresponding production technologies, based
on either open-pond systems or closed photobioreactors. The potential of scientific and technological advances for improvements
in yield and reduction in production costs for carotenoids from microalgae is also discussed. 相似文献
14.
Lignocellulosic biomass contains a variety of carbohydrates, and their conversion into ethanol by fermentation requires an
efficient microbial platform to achieve high yield, productivity, and final titer of ethanol. In recent years, growing attention
has been devoted to the development of cellulolytic and saccharolytic thermophilic bacteria for lignocellulosic ethanol production
because of their unique properties. First of all, thermophilic bacteria possess unique cellulolytic and hemicellulolytic systems
and are considered as potential sources of highly active and thermostable enzymes for efficient biomass hydrolysis. Secondly,
thermophilic bacteria ferment a broad range of carbohydrates into ethanol, and some of them display potential for ethanologenic
fermentation at high yield. Thirdly, the establishment of the genetic tools for thermophilic bacteria has allowed metabolic
engineering, in particular with emphasis on improving ethanol yield, and this facilitates their employment for ethanol production.
Finally, different processes for second-generation ethanol production based on thermophilic bacteria have been proposed with
the aim to achieve cost-competitive processes. However, thermophilic bacteria exhibit an inherent low tolerance to ethanol
and inhibitors in the pretreated biomass, and this is at present the greatest barrier to their industrial application. Further
improvement of the properties of thermophilic bacteria, together with the optimization production processes, is equally important
for achieving a realistic industrial ethanol production. 相似文献
15.
Fialho AM Moreira LM Granja AT Popescu AO Hoffmann K Sá-Correia I 《Applied microbiology and biotechnology》2008,79(6):889-900
Bacterial exopolysaccharides (EPS) are products of biotechnology that are of high interest due to their rheological properties. This is the case of sphingans, a group of structurally related EPS secreted by members of the genus Sphingomonas. Among these, gellan is a multifunctional gelling agent produced in high yields by the non-pathogenic strain Sphingomonas elodea ATCC 31461. In its native form, gellan is a linear anionic EPS based on a tetrasaccharide repeat unit composed of two molecules of D: -glucose, one of L: -rhamnose and one of D: -glucuronic acid. The native gellan is partially esterified with acyl substituents (1 mol of glycerate and 0.5 mol of acetate) per repeat unit. Gellan has unique characteristics and has many applications, particularly in the food, pharmaceutical, and biomedical fields. This review summarizes current knowledge on the structure and properties of gellan and provides details about the biosynthesis of this exopolysaccharide. In addition, a highlight of the importance of gellan in industrial and medicinal applications is given. 相似文献
16.
Artemisinin isolated from the aerial parts of Artemisia annua L. is a promising and potent antimalarial drug which has a remarkable activity against chloroquine-resistant and chloroquine-sensitive strains of Plasmodium falciparum, and is useful in treatment of cerebral malaria. Because the low content (0.01–1 %) of artemisinin in A. annua is a limitation to the commercial production of the drug, many research groups have been focusing their researches on enhancing the production of artemisinin in tissue culture or in the whole plant of A. annua. This review mainly focuses on the progresses made in the production of artemisinin from A. annua by biotechnological strategies including in vitro tissue culture, metabolic regulation of artemisinin biosynthesis, genetic engineering, and bioreactor technology. 相似文献
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Gullo Maria La China Salvatore Falcone Pasquale Massimiliano Giudici Paolo 《Applied microbiology and biotechnology》2018,102(16):6885-6898
Applied Microbiology and Biotechnology - Bacterial cellulose is an attractive biopolymer for a number of applications including food, biomedical, cosmetics, and engineering fields. In addition to... 相似文献
18.
Biosynthesis of cardiolipin in Escherichia coli 总被引:13,自引:0,他引:13
19.
Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives 总被引:1,自引:0,他引:1
Akinori Matsushika Hiroyuki Inoue Tsutomu Kodaki Shigeki Sawayama 《Applied microbiology and biotechnology》2009,84(1):37-53
Bioethanol production from xylose is important for utilization of lignocellulosic biomass as raw materials. The research on
yeast conversion of xylose to ethanol has been intensively studied especially for genetically engineered Saccharomyces cerevisiae during the last 20 years. S. cerevisiae, which is a very safe microorganism that plays a traditional and major role in industrial bioethanol production, has several
advantages due to its high ethanol productivity, as well as its high ethanol and inhibitor tolerance. However, this yeast
cannot ferment xylose, which is the dominant pentose sugar in hydrolysates of lignocellulosic biomass. A number of different
strategies have been applied to engineer yeasts capable of efficiently producing ethanol from xylose, including the introduction
of initial xylose metabolism and xylose transport, changing the intracellular redox balance, and overexpression of xylulokinase
and pentose phosphate pathways. In this review, recent progress with regard to these studies is discussed, focusing particularly
on xylose-fermenting strains of S. cerevisiae. Recent studies using several promising approaches such as host strain selection and adaptation to obtain further improved
xylose-utilizing S. cerevisiae are also addressed. 相似文献
20.
Tissue engineering: current state and perspectives 总被引:23,自引:0,他引:23
Tissue engineering is an interdisciplinary field that involves cell biology, materials science, reactor engineering, and clinical research with the goal of creating new tissues and organs. Significant advances in tissue engineering have been made through improving singular aspects within the overall approach, e.g., materials design, reactor design, or cell source. Increasingly, however, advances are being made by combining several areas to create environments which promote the development of new tissues whose properties more closely match their native counterparts. This approach does not seek to reproduce all the complexities involved in development, but rather seeks to promote an environment which permits the native capacity of cells to integrate, differentiate, and develop new tissues. Progenitors and stem cells will play a critical role in understanding and developing new engineered tissues as part of this approach. 相似文献