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1.
S. NAGAI L. MCCAULEY N. YASUDA D. L. ERDNER D. M. KULIS Y. MATSUYAMA S. ITAKURA D. M. ANDERSON 《Molecular ecology resources》2006,6(3):756-758
Outbreaks of paralytic shellfish poisoning caused by the toxic dinoflagellate Alexandrium minutum (Dinophyceae) are a worldwide concern from both the economic and human health points of view. For population genetic studies of A. minutum distribution and dispersal, highly polymorphic genetic markers are of great value. We isolated 12 polymorphic microsatellites from this cosmopolitan, toxic dinoflagellate species. These loci provide one class of highly variable genetic markers, as the number of alleles ranged from four to 12, and the estimate of gene diversity was from 0.560 to 0.862 across the 12 microsatellites; these loci have the potential to reveal genetic structure and gene flow among A. minutum populations. 相似文献
2.
The outbreak of paralytic shellfish poisoning caused by the toxic dinoflagellate Alexandrium catenella (Dinophyceae) is a worldwide concern from the economic and food hygiene point of view. To assess the dispersal mechanism, a population genetic study using highly polymorphic genetic markers is one of the crucial approaches. We isolated 12 polymorphic microsatellites from this species. These loci provide one class of highly variable genetic marker as the number of alleles ranged from three to 12 and the estimate of gene diversity was from 0.248 to 0.897 across the 12 microsatellites; we consider that these loci have a potential to detail the genetic structure and gene flow among A. catenella populations. 相似文献
3.
Manal A. Al-Kandari Andrea C. Highfield Matt J. Hall Paul Hayes Declan C. Schroeder 《Harmful algae》2011,10(6):636-643
Karenia mikimotoi is a toxic dinoflagellate that is known to form extensive populations in the Eastern North Atlantic and Pacific Oceans that that impacts significantly on recreation and fisheries industries in these areas. Attempts to resolve the complex taxonomy within this “species” have been hampered by the requirement for fine-scale morphological analyses and by the lack of suitable genetic markers. Here we report the use of a novel combination of primer sets designed to facilitate amplification of the rDNA LSU and ITS, and the rbcL genes that can be used to discriminate between K. mikimotoi isolates originating from different geographical regions. We show that isolates from Europe and New Zealand are more closely related to each other than either is to isolates from Japan. Specific PCR-based primers were designed to amplify a region of the rbcL gene for subsequent high resolution analysis of the PCR amplicon melting temperatures. This innovative technique allows us to rapidly discriminate K. mikimotoi from distinct geographic localities and we propose the separation of K. mikimotoi into two distinct sub-species. 相似文献
4.
G. NISHITANI S. NAGAI C. L. LIAN H. YAMAGUCHI S. SAKAMOTO S. YOSHIMATSU K. OYAMA S. ITAKURA M. YAMAGUCHI 《Molecular ecology resources》2007,7(6):1251-1253
We isolated 12 polymorphic microsatellites from the noxious red‐tide‐causing alga Chattonella ovata. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from four to 12, and the observed and expected heterozygosities ranged from 0.238 to 0.850 and from 0.310 to 0.889, respectively. These loci are useful for revealing the genetic structure of and gene flow among C. ovata populations. 相似文献
5.
Changwei Shao Genbo Xu Lei Wang Xiaolin Liao Yongsheng Tian Songlin Chen 《Conservation Genetics》2009,10(3):717-719
Given the ecological and commercial importance of the summer flounder (Paralichthys dentatus), there is a surprising paucity of information on the molecular genetics of this species. Some studies published to date
are concentrated on the reproduction biology. To address this shortcoming, a microsatellite-enriched genomic DNA library of
P. dentatus was generated and screened by sequencing. Twelve dinucleotide microsatellite loci were characterized by genotyping 24 samples.
The observed number of alleles ranged from three to thirteen with an average of 8.25, while the effective number of alleles
ranged from 2.21 to 8.28 with an average of 5.06. The observed and expected heterozygosities ranged from 0.0833 to 0.9583
and from 0.5594 to 0.8980, respectively. Significant deviations from Hardy–Weinberg expectations were detected at three loci
and linkage disequilibrium between two loci was significant after applying Bonferroni correction. In cross-species amplification,
three species showed at least two polymorphic loci. The 12 highly polymorphic microsatellite markers represent a powerful
molecular tool, which will allow for detailed population genetic analyses on this important marine fish. 相似文献
6.
S. NAGAI S. YAMAGUCHI C. L. LIAN Y. MATSUYAMA S. ITAKURA 《Molecular ecology resources》2006,6(2):477-479
We isolated 13 polymorphic microsatellites from the noxious red tide‐causing algae, Heterosigma akashiwo. These loci provide a class of highly variable genetic markers as the number of alleles ranged from three to 12 and the observed and expected heterozygosities ranged from 0.286 to 0.926 and from 0.314 to 0.888, respectively. We consider these loci potentially useful for detailing the genetic structure and gene flow among H. akashiwo populations. 相似文献
7.
We isolated 24 polymorphic microsatellite markers from the toxic dinoflagellate A. tamiyavanichii. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from 2 to 6, and the estimate
of gene diversity was from 0.269 to 0.739 across the 24 microsatellites. We consider that these loci have a potentially to
reveal the genetic structure and gene flow among A. tamiyavanichii populations. 相似文献
8.
G. NISHITANI S. NAGAI S. SAKAMOTO C. L. LIAN C. K. LEE T. NISHIKAWA S. ITAKURA M. YAMAGUCHI 《Molecular ecology resources》2007,7(5):827-829
We isolated 15 polymorphic microsatellites from Cochlodinium polykrikoides. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from two to 15, and the estimate of gene diversity was from 0.083 to 0.880 across the 15 microsatellites. We consider that these loci have a potential to reveal the genetic structure and gene flow among C. polykrikoides populations. 相似文献
9.
Xin Zhan Hai-Yan Hu Cai-Huan Ke Song-Nian Hu De-Xiang Wang Fei Chen 《Conservation Genetics》2009,10(4):1185-1187
Eleven novel microsatellite markers were isolated from small abalone, Haliotis diversicolor Reeve. These loci were tested on 22 individuals from two different geographic populations. We identified a total of 162 alleles
from the 11 microsatellite loci. All of the loci were highly polymorphic. Polymorphism information content (PIC) is ranging
from 0.7276 to 0.9163. Observed and expected heterozygosities ranged from 0.2727 to 1.0000 and from 0.7738 to 0.9429, respectively.
Three loci deviated significantly from Hardy–Weinberg equilibrium. No pairs of loci displayed linkage disequilibrium. These
polymorphic markers will be used to analyze population structure, genetic diversity and construct a genetic linkage map.
Xin Zhan and Hai-Yan Hu contribute equally to this study. 相似文献
10.
11.
S. NAGAI G. NISHITANI S. YAMAGUCHI N. YASUDA C. L. LIAN S. ITAKURA M. YAMAGUCHI 《Molecular ecology resources》2007,7(6):993-995
We isolated 15 polymorphic microsatellites from one of the most noxious red tide‐causing dinoflagellate species, Heterocapsa circularisquama. These loci provide one class of highly variable genetic markers, as the number of alleles ranged from two to six, and the estimate of gene diversity was from 0.205 to 0.684 across the 15 microsatellites. These loci have the potential to reveal genetic structure and gene flow among H. circularisquama populations. 相似文献
12.
S. NAGAI G. NISHITANI S. YAMAGUCHI N. YASUDA C. L. LIAN S. ITAKURA M. YAMAGUCHI 《Molecular ecology resources》2007,7(6):1108-1110
We isolated 15 polymorphic microsatellites from one of the most noxious red tide‐causing dinoflagellate species, Heterocapsa circularisquama. These loci provide one class of highly variable genetic markers, as the number of alleles ranged from two to six, and the estimate of gene diversity from 0.205 to 0.684 across the 15 microsatellites. These loci have the potential to reveal genetic structure and gene flow among H. circularisquama populations. 相似文献
13.
Michael G. Gardner Juan J. Sanchez Rachael Y. Dudaniec Leah Rheinberger Annabel L. Smith Kathleen M. Saint 《Conservation Genetics》2008,9(1):233-237
We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity
ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation
for conservation strategies for the endangered T. adelaidensis. 相似文献
14.
Stuart W. A’Hara Joan Elizabeth Cottrell 《Molecular breeding : new strategies in plant improvement》2009,23(2):349-355
Robust, polymorphic microsatellite DNA markers (simple sequence repeats—SSRs) are valuable tools for a range of tree conservation
and breeding applications. SSRs are routinely used in the study of population genetic structure and diversity, pedigree reconstruction
and genetic linkage mapping. Their abundance in the genome, co-dominant inheritance and potential for cross-species amplification
make microsatellites highly prized markers. This paper characterises 22 novel genomic polymorphic microsatellite loci for
Sitka spruce (Picea sitchensis (Bong.) Carr.). Amplification of DNA from Sitka spruce material was carried out both with a set of unrelated trees to obtain
diversity statistics for each locus, and with the progeny of a full-sib family to test simple Mendelian inheritance. Observed
heterozygosity ranged from 0.38 to 0.91 and allele number per locus ranged from 6 to 21, with a mean of 12.2. In addition,
the primer pairs were tested with DNA from Norway spruce (P. abies) and white spruce (P. glauca) to investigate their potential for cross-species amplification and ten loci amplified in all three species. The results
from these genomic microsatellites are compared to data generated from microsatellites derived from Picea EST libraries. In summary, this novel, highly polymorphic markers represent a significant addition to the rapidly expanding
Picea genomics tool-box.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
We isolated eight polymorphic microsatellites from the red-tide causing microalgae Prorocentrum micans. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from two to six with an average
of 3.9, and the estimate of gene diversity was from 0.15 to 0.55 with an average of 0.41 across the eight microsatellites.
We consider that these loci have a potential to reveal the genetic structure and gene flow among P. micans populations. 相似文献
16.
H. VIGNES M. HOSSAERT‐MCKEY D. BEAUNE D. FEVRE M.‐C. ANSTETT R. M. BORGES F. KJELLBERG M. H. CHEVALLIER 《Molecular ecology resources》2006,6(3):792-795
The animal‐mediated pollination and seed‐dispersal mutualisms of Ficus species give them key roles in tropical ecosystems, but may make them vulnerable to habitat fragmentation. The development of highly polymorphic markers is needed to analyse their genetic diversity and investigate the effects of fragmentation on gene flow. Of thirteen microsatellite loci isolated from Ficus insipida, a monoecious species in French Guiana, eleven were polymorphic (two to six alleles each). High levels of variation were found among loci; expected heterozygosities ranged from 0.151 to 0.715. All markers revealed a broad cross‐species affinity when tested in 23 other Ficus species. 相似文献
17.
Na Chen Yan-Zhuo Yang Xiao-li Yang Xing-xu Zhang Chun-jie Li 《Conservation Genetics》2008,9(4):961-963
Microsatellites are often highly variable and abundant in most complex genomes, therefore are widely used in population genetic
studies. In this study, twelve polymorphic microsatellite loci were isolated and characterized for the Achnatherum inebrians, a plant abundant in grasslands of Northwest China. Characterization of 24 A. inebrians individuals form four geographically distant populations (Gansu, Qinghai, Xinjiang and Inner Mongolia provinces) showed moderate
to high allelic diversity ranging from 3 to 13 alleles per locus, and the expected heterozygosity ranging from 0.41 to 0.67.
No evidence of linkage disequilibrium was found for any locus pairwise comparisons. The markers described here will be useful
for investigating the genetic diversity, genetic structure and gene flow of this species.
Na Chen and Yan-Zhuo Yang contributed equally to this work. 相似文献
18.
Nine highly polymorphic microsatellite markers were developed for Gehyra variegata from an enriched library screened for tetranucleotide repeats. An average of 19.44 alleles per locus and an average observed heterozygosity of 0.86 were found in 81 samples from fragmented populations in the Western Australian wheatbelt. Different gecko species exhibit varying rates of extinction in fragmented landscapes and the genetic markers were developed to test whether these difference are due to different rates of dispersal. The loci were tested for their ability to cross amplify in 12 species of Australian Gekkonidae and Pygopodidae, in an attempt to identify species which might be amenable to population genetic analysis using these microsatellite markers. 相似文献
19.
We report the development of 15 new microsatellite markers for Emys orbicularis and Emys trinacris. A survey of 20 individuals showed that all loci are highly polymorphic with 3–14 alleles per locus. Additionally, 22 Glyptemys muhlenbergii primers were checked for cross-species amplification, with 14 being amplified successfully and polymorphic (2–14 alleles).
A set of eight markers was selected and combined into two multiplex PCRs. Levels of genetic diversity were assessed in 648
individuals covering the complete distribution area. The number of alleles ranged from 13 to 24 and observed heterozygosity
varied between 0.515 and 0.852. 相似文献
20.
Hye Suck An Jung Youn Park Mi-Jung Kim Eun Young Lee Kyung Kil Kim 《Conservation Genetics》2009,10(6):1969-1972
Here we report development and characterization of 14 polymorphic microsatellite loci from Sebastes schlegel. Polymorphism at these loci revealed from 3 to 23 alleles. The observed heterozygosity ranged from 0.34 to 1.00, and the
expected heterozygosity ranged from 0.31 to 0.95. No linkage disequibrium was found. Two loci were significantly deviated
from HWE (P < 0.01). The 14 loci were also surveyed in four other Sebastes species and 12 loci successfully amplified, where allelic diversity ranged from highly polymorphic to monomorphic. These
results demonstrate these microsatellite markers can be used for the study of intra- and inter-specific genetic diversity. 相似文献