共查询到20条相似文献,搜索用时 31 毫秒
1.
Atta Rasool 《Geomicrobiology journal》2013,30(10):854-868
AbstractThe study of microbial communities in river sediments contaminated by thallium (Tl) is necessary to achieve the information for in-situ microbially mediated bioremediation. However, little is known about the microbial community in Tl-contaminated river sediments. In the present study, we characterized the microbial community and their responses to Tl pollution in river sediments from the Tl-mineralized Lanmuchang area, Southwest Guizhou, China. Illumina sequencing of 16S rRNA amplicons revealed that over 40 phyla belong to the domain bacteria. In all samples, Proteobacteria, Cyanobacteria, and Actinobacteria were the most dominant phyla. Based on the UPGMA (Unweighted Pair Group Method with Arithmetic Mean) tree and PCoA (Principal Coordinates Analysis) analysis, microbial composition of each segment was distinct, indicating in-situ geochemical parameters (including Tl, sulfate, TOC, Eh, and pH) had influenced on the microbial communities. Moreover, canonical correspondence analysis (CCA) was employed to further elucidate the impact of geochemical parameters on the distribution of microbial communities in local river sediments. The results indicated that a number of microbial communities including Cyanobacteria, Spirochaete, Hydrogenophaga, and Acinetobacter were positively correlated with total Tl, suggesting potential roles of these microbes to Tl tolerance or to biogeochemical cycling of Tl. Our results suggested a reliable location for the microbial community’s diversity in the presence of high concentrations of Tl and might have a potential association for in-situ bioremediation strategies of Tl-contaminated river. Overall, in situ microbial community could provide a useful tool for monitoring and assessing geo-environmental stressors in Tl-polluted river sediments. 相似文献
2.
The pre-climax epiphytic communities of forests from the Spanish Mediterranean region (Central Plateau) were studied by numerical and traditional floristic methods. One hundred phytosociological relevés were analyzed through a classical numerical approach based on hard partitions improved by PCoA — Principal Coordinates Analysis — ordinations. Two groups easily related to two different suballiances included in Frullanion dilatatae Lecointe 1975, Ulotenion crispae (Barkman 1958) Lecointe 1975 and Fabronienion pusillae Barkman 1958 were detected. Two new syntaxa within the ass. Ortotrichetum lyellii (Allorge 1922) Lecointe 1975 are proposed. Phytogeography, syntaxonomy and ecology of these communities are discussed. 相似文献
3.
Herbivorous beetles comprise a significant fraction of eukaryotic biodiversity and their plant-feeding adaptations make them notorious agricultural pests. Despite more than a century of research on their ecology and evolution, we know little about the diversity and function of their symbiotic microbial communities. Recent culture-independent molecular studies have shown that insects possess diverse gut microbial communities that appear critical for their survival. In this study, we combined culture-independent methods and high-throughput sequencing strategies to perform a comparative analysis of Longitarsus flea-beetles microbial community diversity (MCD). This genus of beetle herbivores contains host plant specialists and generalists that feed on a diverse array of toxic plants. Using a deep-sequencing approach, we characterized the MCD of eleven Longitarsus species across the genus, several of which represented independent shifts to the same host plant families. Database comparisons found that Longitarsus-associated microbes came from two habitat types: insect guts and the soil rhizosphere. Statistical clustering of the Longitarsus microbial communities found little correlation with the beetle phylogeny, and uncovered discrepancies between bacterial communities extracted directly from beetles and those from frass. A Principal Coordinates Analysis also found some correspondence between beetle MCD and host plant family. Collectively, our data suggest that environmental factors play a dominant role in shaping Longitarsus MCD and that the root-feeding beetle larvae of these insects are inoculated by soil rhizosphere microbes. Future studies will investigate MCD of select Longitarsus species across their geographic ranges and explore the connection between the soil rhizosphere and the beetle MCD. 相似文献
4.
The freshwater microbial community in a recreational area of Xochimilco, México was investigated and compared based on spatial
(three different sites) and temporal (dry and rainy seasons) environmental variables. Many of the 16S- and 18S rRNA gene sequences
recovered by DGGE fingerprinting analysis were related to phototrophic microbial phylotypes of known identity. Our genetic
and morphological analysis indicated the ubiquitous presence of the microeukaryotic green algae Desmodesmus-Scenedesmus spp. and of the unicellular cyanobacteria Cyanobium spp. as the most representative populations in the samples. While 18S rRNA-DGGE fingerprinting analysis revealed a homogeneous
community composition across sites and seasons, the 16S rRNA showed significant differences between localities and seasons.
None of the cyanobacteria species with potential to produce toxins were identified across the investigated samples. Correlations
between biotic and abiotic variables evidenced an important difference between the dry and the rainy season, with a greater
consistency in data from the rainy season. According to Principal Component Analysis (PCA), a strong relation between inorganic
nitrogen, species richness, and subaquatic irradiance determines environmental variability in Xochimilco. Complementary and
relevant data in results obtained from microscopy, fingerprinting, and statistical analysis applied in ecology indicate that
a multifaceted approach to the study of microbial communities is necessary to accomplish a comprehensive scientific framework
and to generate proper management strategies. 相似文献
5.
Cornelia Roder Chatchanit Arif Camille Daniels Ernesto Weil Christian R. Voolstra 《Molecular ecology》2014,23(4):965-974
Coral diseases are characterized by microbial community shifts in coral mucus and tissue, but causes and consequences of these changes are vaguely understood due to the complexity and dynamics of coral‐associated bacteria. We used 16S rRNA gene microarrays to assay differences in bacterial assemblages of healthy and diseased colonies displaying White Plague Disease (WPD) signs from two closely related Caribbean coral species, Orbicella faveolata and Orbicella franksi. Analysis of differentially abundant operational taxonomic units (OTUs) revealed strong differences between healthy and diseased specimens, but not between coral species. A subsequent comparison to data from two Indo‐Pacific coral species (Pavona duerdeni and Porites lutea) revealed distinct microbial community patterns associated with ocean basin, coral species and health state. Coral species were clearly separated by site, but also, the relatedness of the underlying bacterial community structures resembled the phylogenetic relationship of the coral hosts. In diseased samples, bacterial richness increased and putatively opportunistic bacteria were consistently more abundant highlighting the role of opportunistic conditions in structuring microbial community patterns during disease. Our comparative analysis shows that it is possible to derive conserved bacterial footprints of diseased coral holobionts that might help in identifying key bacterial species related to the underlying etiopathology. Furthermore, our data demonstrate that similar‐appearing disease phenotypes produce microbial community patterns that are consistent over coral species and oceans, irrespective of the putative underlying pathogen. Consequently, profiling coral diseases by microbial community structure over multiple coral species might allow the development of a comparative disease framework that can inform on cause and relatedness of coral diseases. 相似文献
6.
Arthur Escalas Thierry Bouvier Maud A. Mouchet Fabien Leprieur Corinne Bouvier Marc Troussellier David Mouillot 《Environmental microbiology》2013,15(10):2642-2657
Recent developments of molecular tools have revolutionized our knowledge of microbial biodiversity by allowing detailed exploration of its different facets and generating unprecedented amount of data. One key issue with such large datasets is the development of diversity measures that cope with different data outputs and allow comparison of biodiversity across different scales. Diversity has indeed three components: local (α), regional (γ) and the overall difference between local communities (β). Current measures of microbial diversity, derived from several approaches, provide complementary but different views. They only capture the β component of diversity, compare communities in a pairwise way, consider all species as equivalent or lack a mathematically explicit relationship among the α, β and γ components. We propose a unified quantitative framework based on the Rao quadratic entropy, to obtain an additive decomposition of diversity (γ = α + β), so the three components can be compared, and that integrate the relationship (phylogenetic or functional) among Microbial Diversity Units that compose a microbial community. We show how this framework is adapted to all types of molecular data, and we highlight crucial issues in microbial ecology that would benefit from this framework and propose ready‐to‐use R‐functions to easily set up our approach. 相似文献
7.
Variability of morphological characters and Inter Simple Sequence Repeat (ISSR) markers is studied in a mixed multispecies
sympatric growth of wild roses in Lugansk province of Ukraine. Several methods of data analysis are used, such as Principal
Coordinates Analysis, cluster analysis (UPGMA), and Bayesian analyses of population structure with the Structure 2.2 and NewHybrids
programs. The results indicate that there are four species growing at the site, viz. R. canina, R. villosa, R. dimorpha, and R. podolica, as well as various hybrids between them. The hybrid nature of the specimens is confirmed by molecular data, however they
are not intermediate between the parental species as to their morphology. The hybrids either coincide morphologically with
one of the parents, or demonstrate novel combinations of diagnostic characters. 相似文献
8.
Timothy L Tickle Nicola Segata Levi Waldron Uri Weingart Curtis Huttenhower 《The ISME journal》2013,7(12):2330-2339
Microbial community samples can be efficiently surveyed in high throughput by sequencing markers such as the 16S ribosomal RNA gene. Often, a collection of samples is then selected for subsequent metagenomic, metabolomic or other follow-up. Two-stage study design has long been used in ecology but has not yet been studied in-depth for high-throughput microbial community investigations. To avoid ad hoc sample selection, we developed and validated several purposive sample selection methods for two-stage studies (that is, biological criteria) targeting differing types of microbial communities. These methods select follow-up samples from large community surveys, with criteria including samples typical of the initially surveyed population, targeting specific microbial clades or rare species, maximizing diversity, representing extreme or deviant communities, or identifying communities distinct or discriminating among environment or host phenotypes. The accuracies of each sampling technique and their influences on the characteristics of the resulting selected microbial community were evaluated using both simulated and experimental data. Specifically, all criteria were able to identify samples whose properties were accurately retained in 318 paired 16S amplicon and whole-community metagenomic (follow-up) samples from the Human Microbiome Project. Some selection criteria resulted in follow-up samples that were strongly non-representative of the original survey population; diversity maximization particularly undersampled community configurations. Only selection of intentionally representative samples minimized differences in the selected sample set from the original microbial survey. An implementation is provided as the microPITA (Microbiomes: Picking Interesting Taxa for Analysis) software for two-stage study design of microbial communities. 相似文献
9.
Metagenome skimming for phylogenetic community ecology: a new era in biodiversity research
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It is now well recognized that considering species evolutionary history is crucial for understanding the processes driving community assembly (Cavender‐Bares et al. 2009 ). Considerable efforts have been made to integrate phylogenetics and community ecology into a single theoretical framework. Yet, assessing phylogenetic structure at the community scale remains a great challenge, in particular for poorly known organisms. While DNA metabarcoding is increasingly used for assessing taxonomic composition of complex communities from environmental samples, biases and limitations of this technique can preclude the retrieval of information on phylogenetic community structure. In this issue of Molecular Ecology, Andújar et al. (2015) demonstrate that shotgun sequencing of bulk samples of soil beetles and subsequent reconstruction of mitochondrial genomes can provide a solid phylogenetic framework to estimate species diversity and gain insights into the mechanisms underlying the spatial turnover of soil mesofaunal assemblages. This work highlights the enormous potential of ‘metagenome skimming’ not only for improving the current standards of DNA‐based biodiversity assessment but also for opening up the application of phylogenetic community ecology to hyperdiverse and poorly known biota, which was heretofore inconceivable. 相似文献
10.
With the use of molecular ecology methods, the archaeal component of microbial mats on coral-like structures associated with methane seeps occurring at a depth of about 200 m in the Black Sea was investigated without the isolation of pure cultures. Using archaea-specific 16S rDNA–targeted oligonucleotide primes, long fragments of genes were amplified, cloned, and sequenced and their phylogenetic analysis was carried out. It was shown that archaea in microbial mats on coral-like structures are represented by two dominant phylotypes that belong to the kingdoms Crenarchaeota and Euryarchaeota and are not specifically related to any described archaeal species. The possible role of the revealed archaea in the process of anaerobic methane oxidation is discussed. 相似文献
11.
Arthropod communities on hybrid and parental cottonwoods are phylogenetically structured by tree type: Implications for conservation of biodiversity in plant hybrid zones
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Karl J. Jarvis Gerard J. Allan Ashley J. Craig Rebecca K. Beresic‐Perrins Gina Wimp Catherine A. Gehring Thomas G. Whitham 《Ecology and evolution》2017,7(15):5909-5921
Although hybridization in plants has been recognized as an important pathway in plant speciation, it may also affect the ecology and evolution of associated communities. Cottonwood species (Populus angustifolia and P. fremontii) and their naturally occurring hybrids are known to support different plant, animal, and microbial communities, but no studies have examined community structure within the context of phylogenetic history. Using a community composed of 199 arthropod species, we tested for differences in arthropod phylogenetic patterns within and among hybrid and parental tree types in a common garden. Three major patterns emerged. (1) Phylogenetic diversity (PD) was significantly different between arthropod communities on hybrids and Fremont cottonwood when pooled by tree type. (2) Mean phylogenetic distance (MPD) and net relatedness index (NRI) indicated that communities on hybrid trees were significantly more phylogenetically overdispersed than communities on either parental tree type. (3) Community distance (Dpw) indicated that communities on hybrids were significantly different than parental species. Our results show that arthropod communities on parental and hybrid cottonwoods exhibit significantly different patterns of phylogenetic structure. This suggests that arthropod community assembly is driven, in part, by plant–arthropod interactions at the level of cottonwood tree type. We discuss potential hypotheses to explain the effect of plant genetic dissimilarity on arthropod phylogenetic community structure, including the role of competition and environmental filtering. Our findings suggest that cottonwood species and their hybrids function as evolutionarily significant units (ESUs) that affect the assembly and composition of associated arthropod communities and deserve high priority for conservation. 相似文献
12.
Reysenbach AL Ehringer M Hershberger K 《Extremophiles : life under extreme conditions》2000,4(1):61-67
The use of molecular phylogenetic approaches in microbial ecology has revolutionized our view of microbial diversity at high
temperatures and led to the proposal of a new kingdom within the Archaea, namely, the "Korarchaeota." We report here the occurrence
of another member of this archaeal group and a deeply rooted bacterial sequence from a thermal spring in Yellowstone National
Park (USA). The DNA of a mixed community growing at 83°C, pH 7.6, was extracted and the small subunit ribosomal RNA gene (16S
rDNA) sequences were obtained using the polymerase chain reaction. The products were cloned and five different phylogenetic
types ("phylotypes") were identified: four archaeal phylotypes, designated pBA1, pBA2, pBA3, and pBA5, and only one bacterial
phylotype, designated pBB. pBA5 is very closely related to the korarchaeotal phylotype, pJP27, from Obsidian Pool in Yellowstone
National Park. The pBB phylotype is a lineage within the Aquificales and, based on 16S rRNA sequence, is different enough from the members of the Aquificales to constitute a different genus. In situ hybridization with bacterial-specific and Aquificales-specific fluorescent oligonucleotide probes indicated the bacterial population dominated the community and most likely contributed
significantly to biogeochemical cycling within the community.
Received: August 29, 1999 / Accepted: November 16, 1999 相似文献
13.
Rittmann BE 《Trends in biotechnology》2006,24(6):261-266
Microbial ecology and environmental biotechnology are inherently tied to each other. The concepts and tools of microbial ecology are the basis for managing processes in environmental biotechnology; and these processes provide interesting ecosystems to advance the concepts and tools of microbial ecology. Revolutionary advancements in molecular tools to understand the structure and function of microbial communities are bolstering the power of microbial ecology. A push from advances in modern materials along with a pull from a societal need to become more sustainable is enabling environmental biotechnology to create novel processes. How do these two fields work together? Five principles illuminate the way: (i) aim for big benefits; (ii) develop and apply more powerful tools to understand microbial communities; (iii) follow the electrons; (iv) retain slow-growing biomass; and (v) integrate, integrate, integrate. 相似文献
14.
The development of multispecies oral microbial communities involves complex intra- and interspecies interactions at various
levels. The ability to adhere to the resident bacteria or the biofilm matrix and overcome community resistance are among the
key factors that determine whether a bacterium can integrate into a community. Fusobacterium nucleatum is a prevalent Gram-negative oral bacterial species that is able to adhere to a variety of oral microbes and has been implicated
in playing an important role in the establishment of multispecies oral microbial community. However, the majority of experiments
thus far has focused on the physical adherence between two species as measured by in vitro co-aggregation assays, while the community-based effects on the integration of F. nucleatum into multispecies microbial community remains to be investigated. In this study, we focus on community integration of F. nucleatum. We demonstrated using an established in vitro mice oral microbiota (O-mix) that the viability of F. nucleatum was significantly reduced upon addition to the O-mix due to cell contact-dependent induction of hydrogen peroxide (H2O2) production by oral community. Interestingly, this inhibitory effect was significantly alleviated when F. nucleatum was allowed to adhere to its known interacting partner species (such as Streptococcus sanguinis) prior to addition. Furthermore, this aggregate formation-dependent protection was absent in the F. nucleatum mutant strain ΔFn1526 that is unable to bind to a number of Gram-positive species. More importantly, this protective effect was also observed during
integration of F. nucleatum into a human salivary microbial community (S-mix). These results suggest that by adhering to other oral microbes, F. nucleatum is able to mask the surface components that are recognized by H2O2 producing oral community members. This evasion strategy prevents detection by antagonistic oral bacteria and allows integration
into the developing oral microbial community. 相似文献
15.
以延河流域不同植被区内人工刺槐(Robinia pseudoacacia)群落和乡土植物群落的土壤微生物为研究对象,利用Biolog微平板技术对土壤微生物功能多样性进行测定,分析人工引种刺槐在不同环境梯度(3个植被区)下对土壤微生物功能多样性的影响。结果表明:刺槐对不同环境梯度下的土壤微生物的影响明显不同。从草原区到森林区,刺槐林之间土壤微生物群落的平均颜色变化率(AWCD)和土壤微生物功能多样性指数均没有显著变化;但与乡土植物群落比较,草原区、森林草原区和森林区土壤AWCD分别表现为刺槐乡土植物、刺槐乡土植物、刺槐乡土植物;在草原区和森林草原区刺槐林土壤微生物群落的群落丰富度指数(S)、Shannon-Wiener指数(H)、Simpson指数(D)、Mc Intosh指数(U)均大于乡土植物,森林区刺槐林群落丰富度指数(S)、Shannon指数(H)、Simpson指数(D)均小于乡土植物;刺槐林和乡土植物群落下土壤微生物碳源利用存在差异,主要体现在对糖类、氨基酸类的利用上。PCA分析显示主成分1贡献较大的碳源有24种,在主成分分离中起主要贡献作用的是糖类、氨基酸类和羧酸类。土壤碳氮含量能影响土壤微生物功能多样性指数,土壤含水量和温湿度能够影响碳源的利用类型。刺槐对土壤微生物功能多样性的影响存在区域差异,在评价刺槐对土壤生态过程与功能的影响时必须要考虑这种空间差异性。 相似文献
16.
Microbial mats of twenty-six lakes from Potter Peninsula,King George Island,Antarctica 总被引:3,自引:0,他引:3
The taxonomic composition and ecology of microbial mats were investigated in 26 lentic environments of Potter Peninsula (King George Island, Antarctica) during the summer of 1995/96. These have different limnological characteristics according to the location of the basins. Among the 139 algal taxa registered, 10 are new records for Antarctica. Bacillariophyceae was the dominant class in terms of species richness, followed by the Cyanobacteria and Chlorophyceae. TWINSPAN classification was used to describe six epilithic algal assemblages; Canonical Correspondence Analysis ordination showed that soluble reactive phosphorus, phytoplanktonic chlorophyll a concentration and conductivity were the main sources for the variation in the data. 相似文献
17.
Bicego M Dellaglio F Felis GE 《Journal of bioinformatics and computational biology》2007,5(5):1069-1085
The crucial role played by the analysis of microbial diversity in biotechnology-based innovations has increased the interest in the microbial taxonomy research area. Phylogenetic sequence analyses have contributed significantly to the advances in this field, also in the view of the large amount of sequence data collected in recent years. Phylogenetic analyses could be realized on the basis of protein-encoding nucleotide sequences or encoded amino acid molecules: these two mechanisms present different peculiarities, still starting from two alternative representations of the same information. This complementarity could be exploited to achieve a multimodal phylogenetic scheme that is able to integrate gene and protein information in order to realize a single final tree. This aspect has been poorly addressed in the literature. In this paper, we propose to integrate the two phylogenetic analyses using basic schemes derived from the multimodality fusion theory (or multiclassifier systems theory), a well-founded and rigorous branch for which its powerfulness has already been demonstrated in other pattern recognition contexts. The proposed approach could be applied to distance matrix-based phylogenetic techniques (like neighbor joining), resulting in a smart and fast method. The proposed methodology has been tested in a real case involving sequences of some species of lactic acid bacteria. With this dataset, both nucleotide sequence- and amino acid sequence-based phylogenetic analyses present some drawbacks, which are overcome with the multimodal analysis. 相似文献
18.
János Podani 《Plant Ecology》1989,83(1-2):111-128
The methodology of comparing the results of multivariate community studies (resemblance matrices, ordinations, hierarchical and nonhierarchical classifications) is reviewed from two viewpoints: basic strategy and measure employed. The basic strategy is determined by 7 choices concerning the type of results, consensus methods or resemblance measures, hypothesis testing or exploratory analysis, lack or presence of reference basis, data set congruence or algorithmic effects, number of factors responsible for differences among results, and the number of properties considered in the comparison. Included is a brief summary of methods applicable to vegetation studies. Examples from a grassland survey demonstrate the utility of comparisons in evaluating the effects of plot size, data type, standardization, taxonomic level and number of species on classifications and ordinations.Abbreviations OUC =
Operational Unit of Comparison
- PCA =
Principal Components Analysis
- PCoA =
Principal Coordinates Analysis
- SSA =
Incremental Sum of Squares Agglomeration 相似文献
19.
The anaerobic ammonium oxidation process is a new process for ammonia removal from wastewater. It is also a new microbial
physiology that was previously believed to be impossible. The identification of Candidatus Brocadia anammoxidans and its relatives as the responsible bacteria was only possible with the development of a new experimental
approach. That approach is the focus of this paper. The approach is a modernisation of the Winogradsky/Beyerinck strategy
of selective enrichment and is based on the introduction of the molecular toolbox and modern bioreactor engineering to microbial
ecology. It consists of five steps: (1) postulation of an ecological niche based on thermodynamic considerations and macro-ecological
field data; (2) engineering of this niche into a laboratory bioreactor for enrichment culture; (3) black-box physiological
characterisation of the enrichment culture as a whole; (4) phylogenetic characterisation of the enriched community using molecular
tools; (5) physical separation of the dominant members of the enrichment culture using gradient centrifugation and the identification
of the species of interest in accordance with Koch's postulates; (6) verification of the in situ importance of these species in the actual ecosystems. The power of this approach is illustrated with a case study: the identification
of the planctomycetes responsible for anaerobic ammonium oxidation. We argue that this was impossible using molecular ecology
or conventional ‘cultivation based techniques’ alone. We suggest that the approach might also be used for the microbiological
study of many interesting microbes such as anaerobic methane oxidisers.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
20.
Pedro A. Sosa Miguel A. González-Pérez Claudio Moreno Jake B. Clarke 《Conservation Genetics》2010,11(6):2357-2368
Five polymorphic microsatellites (simple sequence repeat; SSR) markers were used to estimate the levels of genetic variation
within and among natural populations from different islands of the endangered endemic from the Canary Islands Sambucus palmensis Link (Sambucaceae). Genetic data were used to infer potential evolutionary processes that could have led to present genetic
differentiation among islands. The levels of genetic variability of S. palmensis were considerably high; proportion of polymorphic loci (P = 100%), mean number of alleles per locus (A = 6.8), average expected
heterozygosity (He = 0.499). In spite of its small population size and endemic character, 58 different multilocus genotypes
were detected within the 165 individuals analyzed. All samples located in different islands always presented different multilocus
genotypes. Principal Coordinates Analysis, genetic differentiation analysis (F
ST and G
ST
′) and Bayesian Cluster Analysis revealed significant genetic differences among populations located in different islands. However,
this genetic differentiation was not recorded among Tenerife and La Gomera populations, possibly revealing the uncontrolled
transfer of material between both islands. AMOVA analysis attributed 77% of the variance to differences within populations,
whereas 8% was distributed between islands. The levels of genetic differentiation observed among populations, and the genetic
diversity distribution within populations in S. palmensis, indicate that management should aim to conserve as many of the small populations as possible. Concentrating conservation
efforts only on the few large populations would result in the likelihood of loss of genetic variability for the species. 相似文献