Testing DNA barcoding in closely related groups of Lysimachia L. (Myrsinaceae)

It has been suggested that rbcL and matK are the core barcodes in plants, but they are not powerful enough to distinguish between closely related plant groups. Additional barcodes need to be evaluated to improve the level of discrimination between plant species. Because of their well-studied taxonomy and extreme diversity, we used Chinese Lysimachia (Myrsinaceae) species to test the performance of core barcodes (rbcL and matK) and two additional candidate barcodes (trnH-psbA and the nuclear ribosomal ITS); 97 accessions from four subgenus representing 34 putative Lysimachia species were included in this study. And many closely related species pairs in subgen. Lysimachia were covered to detect their discriminatory power. The inefficiency of rbcL and matK alone or combined in closely related plant groups was validated in this study. TrnH-psbA combined with rbcL + matK did not yet perform well in Lysimachia groups. In contrast, ITS, alone or combined with rbcL and/or matK, revealed high resolving ability in Lysimachia. We support ITS as a supplementary barcode on the basis of core barcode rbcL and matK. Besides, this study also illustrates several mistakes or underlying evolutionary events in Lysimachia detected by DNA barcoding.     

五种珍珠菜的核型研究

本文对５种珍珠菜亚属植物进行了染色体数目及核型研究。其中瓣珍珠菜２ｎ＝２４＝１２ｍ＋８ｓｍ＋４ｓｔ（２ＳＡＴ）、黑腺珍珠菜２ｎ＝２２＝２ｍ＋４ｓｍ＋６ｓｔ＋１０ｔ、泽珍珠菜２ｎ＝２４＝１４ｍ＋６ｓｍ（２ＳＡＴ）＋４ｓｔ和小叶珍珠菜２ｎ＝４８＝３４ｍ＋１０ｓｍ＋４ｓｔ的染色体数目及核型为首次报道。中国九江产的红根草核型２ｎ＝２４＝２０ｍ＋４ｓｍ（２ＳＡＴ）与日本产的核型２ｎ＝２４＝１８ｍ（１ＳＡＴ）     

珍珠菜属(报春花科)一新种

描述了珍珠菜属(报春花科)一新种, 即祁门过路黄(Lysimachia qimenensis X.H.Guo, X.P.Zhang et J.W.Shao);并对祁门过路黄与其近缘种巴东过路黄(Lysimachia patungensis Hand.-Mazz.)的核型进行了报道。     

龙胜香草的名实订正

近年发表的报春花科Primulaceae龙胜香草Lysimachia longshengensis G. Z. Li &; S. C. Tang实为茄科Solanaceae单花红丝线Lycianthes lysimachioides (Wall.) Bitter之同物异名,故予以归并。     

珍珠菜属植物叶表皮纹饰特征及其系统学意义

利用光学显微镜和扫描电镜对21种珍珠菜属植物叶表皮纹饰特征进行了研究。根据叶表皮外壁纹饰特征,可以明显的划分为两种类型,即黑腺珍珠菜型和细梗香草型。这两种类型与以雄蕊群的构造为主要依据的属下分类系统的对应关系不密切,表明叶表皮纹饰特征在亚属级水平上分类学意义不大。但该属植物叶腺体的有无,颜色和分布以及叶表皮纹饰特征在种内相当稳定,种间差异较大,在种级水平上具有重要的分类学意义。     

安徽3种维管植物新记录

报道安徽省新分布1种蕨类顶芽狗脊[Woodwardia unigemmata (Makino) Nakai],以及2种被子植物广西过路黄(Lysimachia alfredii Hance)和见血青[Liparis nervosa (Thunb.) Lindl.]。标本藏于安徽中医药大学中药标本中心药用植物标本室(ACM)。     

珍珠菜属八种植物的花粉形态研究

利用扫描电子显微镜,首次对珍珠菜属(LysimachiaL.)8种植物的花粉形态进行了观察研究,报道了花粉形态特征,探讨了花粉形态特征在分类学上的意义。结果表明:该属花粉粒大小和外壁雕纹可作为分种的依据。     

Molecular phylogeny of Lysimachia (Myrsinaceae) based on chloroplast trnL-F and nuclear ribosomal ITS sequences

Both nuclear ribosomal ITS and chloroplast trnL-F sequences were acquired for 57 species (accessions) of Lysimachia and its close relatives, and were analyzed together with sequences retrieved from databases. The results of phylogenetic analyses based on these data (separately or combined) show that Lysimachia is paraphyletic, with the monotypic genus Glaux nested deeply inside. Previous suggestions that Anagallis and Trientalis could be ingroups of Lysimachia were not corroborated by our results. The molecular phylogenies do not support the current infrageneric divisions of Lysimachia. Subgenus Lysimachia contains at least five independent lineages. The Hawaii endemic subgenus Lysimachiopsis was shown to group with subgenera Palladia and Heterostylandra, instead of subgenus Idiophyton as previously suggested. The two North American representatives of Lysimachia, subgenus Seleucia and section Verticillatae of subgenus Lysimachia are group together as the most basal clade of the genus. Parallel and independent evolutions were inferred for morphological characters that were previously used as diagnostic criteria. Molecular phylogenies do not offer clear inferences on the overall historical biogeography of Lysimachia, but Southeast Asia origins of several clades, including the Hawaiian endemic clade and the Iberian Lysimachia ephemerum are strongly supported.     

5种珍珠菜属植物的核型分析

对 5种珍珠菜属 (LysimachiaL .)植物的核型进行了研究 ,其中巴东过路黄 2n =2x =2 4 =6m 4sm 6st 8t、光叶巴东过路黄 2n =2x =2 4 =6m 4sm 6st 8t、临时救 2n =2x =2 4=2m 4sm 4st 14t的核型属首次报道。过路黄 2n =2x =2 4 =2m 4sm 6st 12t和星宿菜2n =2x =2 4 =2 0m 4sm与前人报道的一致。本文还对该属已报道的 17种植物的核型资料进行了总结和比较分析 ,对珍珠菜属植物的核型进化方向作了初步推测。另外对一存疑过路黄(2n =2x =2 4 =2m 6sm 4st 12t)与其近缘种的核型进行了比较研究。     

珍珠菜属三种植物的核型研究

对国产三种珍珠菜属 (Lysimachia)植物进行了核型研究 ,其中点腺过路黄 (LysimachiahemsleyanaMaxim .)染色体核型 2n =2 2 =2m +4sm +8st+8t,聚花过路黄 (L .congestifloraHesmsl.)核型 2n =2 4=2m +2sm +1 0st+1 0t及山萝过路黄 (L .melampyroidesR .Knuth)染色体数目 2n =2 2 ,核型 2n =2 2 =4m +6sm +4st+8t,为首次报道。本文还分析了黄连花亚属 (subgen.Lysimachia) 2组 8种植物的核型 ,结果表明黄连花组(sect.Lysimachia)核型类型 1A ,过路黄组 (sect.Nummularia)核型类型 3A或 3B。     

珍珠菜属植物种皮微形态特征及其系统学意义

利用光学显微镜和扫描电镜对11种珍珠菜属植物种皮微形态特征进行了研究。根据种皮微形态特征,可以明显的划分为两种类型,即黑腺珍珠菜型和金爪儿型。珍珠菜亚属的7种植物均属于黑腺珍珠菜型,黄连花亚属的4种植物均属于金爪儿型。种皮微形态特征支持以雄蕊群的构造为主要依据的属下分类系统,在亚属级水平具有重要的分类学意义。     

罗霄山脉东坡—江西省种子植物新记录

报道江西省种子植物1个新记录属萼脊兰属Sedirea Garay et Sweet,及4个新记录种短茎萼脊兰Sedirea subparishii (Z. H. Tsi) Christenson、白花过路黄Lysimachia huitsunae Chien、黄脉莓Rubus xanthoneurus Focke ex Diels、桂东锦香草Phyllagathis guidongensis K. M. Liu & J. Tian,均发现于罗霄山脉东坡。文中简述各新记录种区系地理学意义。     

广东植物区系新资料

报道了广东7个新记录种,即绣球藤、尾囊草、栀子皮、白花过路黄、曲轴黑三棱、线叶玉凤花、丝瓣玉凤花。其中黑三棱科为广东新记录科;尾囊草属、栀子皮属及黑三棱属为广东新记录属。     

海南植物增补（X）

报道海南岛4种新记录植物,包括广防己Aristolochia fangchi Y．C．Wu ex L.D．Chow et S．M．Hwang、花叶秋海棠Begonia cathayana Hemsl．、细梗香草Lysimachia capillipes Hemsl．和毛萼山珊瑚Galeola lindleynan （Hook．f.et Thoms．）Rchb．f.。引用的标本全部存放在中国科学院华南植物园标本馆（IBSC）。     

海南植物增补(Ⅹ)

报道海南岛4种新记录植物,包括广防己Aristolochia fangchi Y.C.Wu ex L.D.Chow et S.M.Hwang、花叶秋海棠Begonia cathayana Hemsl.、细梗香草Lysimachia capillipes Hemsl.和毛萼山珊瑚Galeola lindleyana(Hook.f.etThoms.)Rchb.f.。引用的标本全部存放在中国科学院华南植物园标本馆(IBSC)。     

Zooplankton and environmental factors of a recovering eutrophic lake (Lysimachia Lake, Western Greece)

The present study investigates the zooplankton community dynamics and the abiotic environment in the eutrophic Lake Lysimachia (western Greece). The lake is considered to be recovering from eutrophication after the termination of an urban sewage inflow in 2000, and its waters are replenished constantly from the nearby oligotrophic Lake Trichonis. The results show that, although a decrease in nutrient concentrations was observed compared to the past, the lake still has eutrophic characteristics. This was reflected in the zooplankton community which is typical of those found in eutrophic lakes where rotifers prevail. Similarities among this lake and other nearby lakes were found considering the zooplankton community composition and seasonal variation. However, Lake Lysimachia is inhabited also by a number of different and even unique species (e.g., Moina micrura), suggesting that this ecosystem may be an important biodiversity refuge. Most of the zooplankton species were correlated with water temperature and, to a lesser extent, eutrophication key-water quality variables. Although there are few available data on the zooplankton of the lake, the abundance and composition of the community presenting characteristics indicative of intermediate trophic conditions and suggesting that the lake is probably under a kind of “biological” recovery.     

珍珠菜属四种植物的核型分析

对珍珠菜属4种植物的核型进行了研究。结果表明,叶头过路黄(Lysimachia phyllocephala Hand.-Mazz.)的核型为:2n=2x=24=2m 6sm 2st 14t,茂汶过路黄(L. stellarioidesHand.-Mazz.)的核型为:2n=2x=24=4m 2sm 18t,均属首次报道。矮桃(L. clethroidesDuby)的核型为:2n=2x=24=16m 8sm(1SAT),腺药珍珠菜(L. stenosepala Hemsl.)的核型为:2n=2x=24=10m 12sm 2st,与前人报道的有所不同。还对该属已报道的23种植物的核型进行了比较。     

陕西省种子植物区系新记录

报道了陕西省种子植物区系2新记录属[茜草科(Rubiaceae)的虎刺属(Damnacanthus Gaertn.f.),桔梗科(Campanulaceae)的刺萼参属(Echinocodon Hong)],3新记录种[四川虎刺(Damnacanthus officinarum Huang),刺萼参(Echinocodon lobophyllus Hong)及报春花科(Primulaceae)的异花珍珠菜Lysimachia crispidens(Hance)Hemsl.]。     

Permanent genetic resources added to Molecular Ecology Resources Database 1 December 2010-31 January 2011

This article documents the addition of 238 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Alytes dickhilleni, Arapaima gigas, Austropotamobius italicus, Blumeria graminis f. sp. tritici, Cobitis lutheri, Dendroctonus ponderosae, Glossina morsitans morsitans, Haplophilus subterraneus, Kirengeshoma palmata, Lysimachia japonica, Macrolophus pygmaeus, Microtus cabrerae, Mytilus galloprovincialis, Pallisentis (Neosentis) celatus, Pulmonaria officinalis, Salminus franciscanus, Thais chocolata and Zootoca vivipara. These loci were cross-tested on the following species: Acanthina monodon, Alytes cisternasii, Alytes maurus, Alytes muletensis, Alytes obstetricans almogavarii, Alytes obstetricans boscai, Alytes obstetricans obstetricans, Alytes obstetricans pertinax, Cambarellus montezumae, Cambarellus zempoalensis, Chorus giganteus, Cobitis tetralineata, Glossina fuscipes fuscipes, Glossina pallidipes, Lysimachia japonica var. japonica, Lysimachia japonica var. minutissima, Orconectes virilis, Pacifastacus leniusculus, Procambarus clarkii, Salminus brasiliensis and Salminus hilarii.