共查询到20条相似文献,搜索用时 31 毫秒
1.
William B. Wehrenberg Nicholas Ling Paul Brazeau Fred Esch Peter Böhlen Andrew Baird Shao Ying Roger Guillemin 《Biochemical and biophysical research communications》1982,109(2):382-387
The synthetic replicate of a 44 amino acid peptide isolated from a human pancreatic tumor which had caused acromegaly possesses high specific activity to release growth hormone (GH) in anesthetized male rats. The GH secretion induced by this peptide is dose-dependent from 50 ng to 1 μg, with plasma GH concentrations increasing more than 10-fold within 5 min of iv administration at the higher doses. Two enzymatic degradation products of the 44 residue peptide were also isolated and consist of the first 37 and 40 amino acids. All three peptides appear to possess similar potency, on a molar basis, , contrary to results. The specificity of these peptides on GH release was shown by their failure to alter plasma concentrations of prolactin (PRL), thyroid-stimulating hormone (TSH), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and corticosterone. Based on these results, the three peptides with serve as powerful tools with which to investigate the mechanisms of GH secretion. 相似文献
2.
R Wallin R Selset K Sletten 《Biochemical and biophysical research communications》1978,81(4):1319-1328
Three chromophoric peptides have been isolated and characterized from tryptic digests of the α subunit of C-phycocyanin from ,. The amino acid sequences revealed that one phycocyanobilin was ester bond by a tyrosine residue, and another was most probably attached by a thioether linkage. Structural studies of the third chromophoric peptide gave no evidence of how the phycocyanobilin was attached. 相似文献
3.
Benedict G. Archer 《Analytical biochemistry》1981,113(2):439-443
A gel filtration method has been developed for the complete removal of sodium dodecyl sulfate (SDS) from proteins and peptides. The protein or peptide (20 μg–10 mg) containing SDS (up to 30–60 mg) is dissolved in a mixture of propionic acid, formic acid, and water (2:1:2, ). Under these conditions, protein-SDS (or peptide-SDS) complexes, as well as SDS micelles, are dissociated. Subsequently, protein and SDS can be separated on a small Sephadex G-25 superfine column. The recovery of protein is typically 90% or more. 相似文献
4.
Monica Galliano Lorenzo Minchiotti Paolo Osadarola Monica Stoppini Piero Giagnoni Scott Watkins Jeanne Madison Frank W. Putnam 《生物化学与生物物理学报:疾病的分子基础》1993,1225(1):27-32
Albumin Ortonovo is a slow moving variant of human serum albumin which has been found only in people coming from the small villages of Ortonovo and Nicola (Liguria, Italy) and reaches polymorphic frequency (≥1%) in the poorly admixed population group living in that area. This is the first report of a ‘private’ varint detected in a Caucasin population. It probably originated as a mutation in a founder individual many generations ago. Isoelectric focusing analysis of CNBr fragments from the purified variant localized the mutation in fragment CNBr (residues 447–548). This fragment was isolated on a preparative scale by reversed-phase HPLC and subjected to V8 proteinase digestion. Sequence analysis of the abnormal V8 peptide revealed that the variant arises from a previously unreported substitution at position 505 where glutamic acid has been replaced by lysine. The protein data were confirmed by DNA sequence analysis which indicated a single nucleotide change of in the corresponding codon of the structural gene. Since the amino acid substitution found in albumin Ortonovo accords with its electrophoretic mobility on cellulose acetate, residue 505 is probably exposed to the solvent. The clustering of the mutations in the intersubdomain connection linking subdomains IIIA and IIIB (residues 492–511) accords with the fact that this region lies on the molecular surface and is accessible to solvent. 相似文献
5.
Stimulation by interferon of induction of differentiation of human promyelocytic leukemia cells 总被引:3,自引:0,他引:3
F1-ATPase was isolated from yeast . The constituent subunits 1 and 2 were purified by gel permeation chromatography, and their amino acid compositions determined. Both subunits have a similar composition except for cystine, methionine, leucine, histidine, and tryptophan. When F1 is treated for three hours with 5′-p-[3H]fluorosulfonylbenzoyl adenosine in dimethylsulfoxide, 90% of the activity is lost. Disc gel electrophoresis of the modified complex showed that over 90% of the label was associated with subunit 2. A labelled peptide from a digest of subunit 2 was isolated and sequenced. It had the following amino acid sequence: His-Try1-Asp-Val-Ala-Ser-Lys-Val-Gln-Glu, whereby Tyr1 is the modified amino acid residue. This sequence shows homology to other sequences obtained from maize, beef heart, and F1-ATPases. 相似文献
6.
Susan Walker Farmer W. Craig Clarke Harold Papkoff Richard S. Nishioka Howard A. Bern 《Life sciences》1975,16(1):149-158
A protein fraction containing prolactin activity from the pituitary tissue of a teleost fish, , has been purified by a combination of ion-exchange and exclusion chromatographic procedures. The purified prolactin was characterized by disc gel electrophoresis, amino-terminal group identification, and amino acid analysis. Its amino acid composition was found to be similar to ovine prolactin. The purified fish prolactin was found to be 40–50 times more potent than ovine prolactin in the sodium-retaining bioassay. However, it was found to be devoid of yellow pigment-dispersing activity which was previously thought to be a property of teleost prolactin. 相似文献
7.
Yoshihito Yamamoto Chizuko Yanaihara Yumiko Katsumaru Tohru Mochizuki Akihiro Tobe Mitsuo Egawa Hiroo Imura Shosaku Numa Noboru Yanaihara 《Regulatory peptides》1983,6(2):163-168
The carboxy-terminal nonacosapeptide sequence of porcine preproenkephalin B contains the sequence of Leu-enkephalin at its amino terminus. The endogenous existence of this peptide, leumorphin, has not yet been proved. Synthesis of leumorphin was carried out by a solid-phase technique and the purity and structure of the synthetic peptide were confirmed. Synthetic porcine leumorphin exhibited a dose-dependent opiate effect (ED50 4.70 · 10?9 M) on electrically stimulated contraction of the guinea pig ileum preparation. The potency was about 100 times as high as that of Leu-enkephalin. Leumorphin was less potent than dynorphin(1–13) (ED50 0.38 · 10?9 M) but it was more active than (ED50 18 · 10?9 M). The opiate activity was only partially reversed by naloxone. Intracisternal injection of synthetic leumorphin caused significant analgesia in mice (ED50 7.31 nmol/mouse). The potency was lower than that of (ED50 0.60 nmol/mouse) but higher than that of dynorphin(1–13) (ED50 16.10 nmol/mouse). Intracisternally injected leumorphin did not produce such a violent behavioral effect as did dynorphin(1–13), and it exhibited a mild sedative effect. The data supports the concept that leumorphin is a new type of opioid peptide and that the synthetic preparation will be useful for further biological and immunological studies on this peptide. 相似文献
8.
Indentification of two somatomedin A active polypeptides and in vivo effects of a somatomedin A concentrate 总被引:5,自引:0,他引:5
L Fryklund K Uthne H Sievertsson 《Biochemical and biophysical research communications》1974,61(3):957-962
The first chemical characterization of two polypeptides from human serum which stimulate the incorporation of 35S sulfate into chick cartilage is described. These two polypeptides, designated Somatomedin A1 and A2 have a molecular weight of approximately 7000. Although each peptide contains 1 cysteine residue and has asparagine as amino terminal residue, there are apparent differences in the amino acid composition. Administration of a Somatomedin A concentrate to hypophysectomized rats gave an increase in tibial width similar to that obtained with 20 μg human growth hormone. 相似文献
9.
When 30 S ribosomal subunits are irradiated with ultraviolet light, we have found that an RNA-protein crosslinking reaction occurs whose primary target is protein S7. This paper describes the identification of the oligopeptide and oligonucleotide at the crosslinking point, by direct analysis (a) of the peptide remaining attached to an oligonucleotide (after total digestion of the RNA in the crosslinked complex with ribonucleases A and T1, followed by digestion with trypsin), and (b) of the nucleotides remaining attached to the crosslinked protein (after digestion of the RNA in the complex with ribonuclease T1 alone).The crosslinking site was found to lie within a single short peptide, Ser-Met-Ala-Leu-Arg (positions 113 to 117 in the S7 sequence), with methionine as the probable amino acid concerned. The principal RNA site was found to lie within an oligonucleotide three to six bases long, the underlined portion of the partially ordered sequence in section P of the 16 S RNA. The methodology involved has been designed with a view to being generally applicable in future RNA-protein crosslinking studies, where several proteins are simultaneously attached to the RNA. 相似文献
10.
The pharmacologic activity of the hexapeptide fragment corresponding to the amino acid fragment 60–65 in β-lipotropin, (β-LPH-(60–65)) was studied and . In binding assays on synaptosomal plasma membrane the peptide was found to be equipotent to met-enkephalin, but behaved differently to cations; in contrast to met-enkephalin both Mn+2 and Na+ enhanced the binding of β-LPH-(60–65) to synaptosomal plasma membrane. On both the quinea pig ileum and mouse vas deferens β-LPH-(60–65) inhibited contractions elicited by electrical stimulation and each effect was reversible by naloxone. On the guinea pig ileum β-LPH-(60–65) was equipotent to met-enkephalin and 0.5 as potent as normorphine but on the vas deferens it was 4.6 times more potent than normorphine. The activities of β-LPH-(60–65) appear to be due to the intact compound rather than to its conversion to met-enkephalin, since the peptide extracted from the ileum assay was found to behave identically as β-LPH-(60–65) with high pressure liquid chromatography. When β-LPH-(60–65) was administered centrally to mice and rats, no overt central actions were observed and an antinociceptive effect could not be demonstrated. Nor did β-LPH-(60–65) antagonize morphine action or precipitate the withdrawal syndrome in morphine dependent animals. It is concluded that the good agreement which generally exists between and assay procedures for opiate-like activity of morphine and its surrogates does not necessarily hold for the endogenous peptides with similar actions. 相似文献
11.
12.
A peptide released from plasma fibrin stabilzing factor in the conversion to the active enzyme by thrombin 总被引:1,自引:0,他引:1
Y Mikuni S Iwanaga K Konishi 《Biochemical and biophysical research communications》1973,54(4):1393-1403
A peptide, which was released accompanying with the activation of bovine plasma fibrin stabilizing factor (FSF) by thrombin, was isolated and characterized. The peptide consisted of Asp4, Thr3, Ser4, Glu4, Pro5, Gly4, Ala4, Val2, Ile1, Leu2, Phe1, and Arg3. The content of proline was highest in all of these amino acids. The carboxyl-terminal residue of the peptide was identified as arginine. However, no N-terminal amino acid reactive with phenylthiocyanate and dansyl chloride could be determined. Edman degradation on the inactive FSF showed glutamic acid or glutamine as one N-terminal residue. After the activation of FSF by thrombin, glycine was identified as a second N-terminal residue, in addition to glutamic acid (glutamine).These results indicate that the transformation of FSF to the active enzyme by thrombin involves proteolysis of an arginyl-glycyl bond located in the N-terminal region of one of the subunits of the proenzyme. 相似文献
13.
The amino acid sequence of desulforedoxin, a new type of non heme iron protein from Desulfovibrio gigas 总被引:3,自引:0,他引:3
M Bruschi I Moura J Le Gall A V Xavier L C Sieker P Couchoud 《Biochemical and biophysical research communications》1979,90(2):596-605
A new type of non heme iron protein called desulforedoxin has been isolated from the sulfate reducing bacterium, . The complete amino acid sequence has been established. The 36 amino acid residues of the sequence are aligned with the aid of peptides obtained by cyanogen bromide cleavage and by hydrolysis with a peptidase isolated from . Desulforedoxin has been described as a non heme iron protein of molecular weight 7,600 with 2 iron atoms linked to eight cysteine residues. In fact, sequence elucidation shows that it consists of a dimer of a peptide containing 36 aminoacids. We do not know whether if each monomer contains 1 iron atom linked to 4 cysteine residues or whether the two iron cross link the two monomers. Additional studies on the elucidation of the structure of this new cluster are presently under study. 相似文献
14.
Charles M. Stagg Milton S. Feather 《Biochimica et Biophysica Acta (BBA)/General Subjects》1973,320(1):64-72
Exhaustive extraction of the cell walls of Aspergillus niger with 10% NaOH solution leaves an alkali-resistant residue containing chitin and glucan as the major components. The glucan in this residue comprises 58.7% of the total cell wall glucan and was characterized by permethylation, and identification of the resulting obtained after hydrolysis by gas-liquid chromagtography and mass spectrometry of the derived partially acetylated, partially methylated, [1-2H]alditols. The glucan was separated from the chitin by acetylation of the alkali-resistance material, a procedure which separates a large portion of the total glucan as a chloroformsoluble acetate, abd by treatment of the alkali-insoluble residue with nitrous acid, a procedure which was found to render the complex soluble in dimethylsulfoxide and amenable, therefore, to permethylation. The data collected suggests that the preparation is an essentially linear glucan containing 85–95% 1 → 3 linkages and 10–15% 1 → 4 linkages. An analysis of the glycosidic linkages using NMR spectroscopy indicate that both α and β linkages are present in the ratio of 4:1. An identical glucan appears to be present in the cell walls of Penicillium chrysogenum as well as the spore cell walls of both organisms, as evidenced by methylation studies. 相似文献
15.
A K Koul G F Wasserman P K Warme 《Biochemical and biophysical research communications》1979,89(4):1253-1259
New semi-synthetic peptide analogs of horse heart cytochrome have been prepared by replacing tyrosine at position 67 by phenylalanine and L-parafluorophenylalanine. The former has 56% biological activity in the succinate oxidase system whereas the parafluorophenylalanine analog is totally inactive. The tyrosine at position 74 has also been replaced by leucine. It shows a high level of biological activity (58%), which demonstrates that the presence of an aromatic residue at this position is not required for electron transfer. 相似文献
16.
Data are presented on the isolation, biosynthesis, and identification of a small peptide (H) from parathyroid gland. Under our experimental conditions this peptide (H) represents, in addition to secretory protein-I and proparathyroid hormone, the other major protein which is rapidly synthesized during shorterm incubations of tissue slices. N-terminal sequence analysis was performed on samples of peptide H and the resulting data used to conduct a search of the sequence data bank. The search established the identity of peptide H as ubiquitin. These findings establish parathyroid gland as another system which rapidly produces ubiquitin , in addition to the systems employing hypothalamus and pituitary where ubiquitin biosynthesis was initially observed by Seidah and Scherrer . 相似文献
17.
Makoto Ito Tatsuya Yamagata 《Biochimica et Biophysica Acta (BBA)/General Subjects》1984,801(3):381-387
The linkage of teleost skin keratan sulfate to protein was investigated. Afeter its exhaustive digestion with pronase, peptidokeratan sulfate was obtained with aspartic acid as the predominant amino acid. The N-terminal of the amino acid residues of the preparation was dansylated, and the carbohydrate-peptide linkage fragment was isolated, with the aid of fluorescence, by sequential digestion with Flavobacterium endo-β-galactosidase, β-galactosidase, β-N-acetylhexosaminidase and endo-β-N-acetylglucosaminidase D, followed by Bio-Gel p-4 column chromatography. The structure of the dansylated fragment thus obtained was identified dansylated asparaginyl . Treatment of the dansylated keratan sulfate peptide with almond glycopeptidase, which specially cleaves thet asparaginyl linkage in the glycoproteins, also showed asparaginyl linkage to be in the core region of this keratan sulfate. We conclude that teleost skin keratan sulfate is bound to protein via an N-glycosyl linkage between and asparagine. The keratan sulfate core apparently consist of trimannosyl-N,N′-diacetylchitobiose units, considering the specificity of endo-β-N-acetylglucosaminidase D. 相似文献
18.
Mayte Villalba Isabel Varela Isabel Mérida María A. Pajares Alvaro Martínez del Pozo JoséM. Mato 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1985,847(3):273-279
The present results show that the catalytic subunit of cyclic AMP-dependent protein kinase phosphorylates the 50 kDa protein of rat liver phospholipid methyltransferase at one single site on a serine residue. Phosphorylation of this site is stimulated 2- to 3-fold by S-adenosylmethionine. S-adenosylmethionine-dependent protein phosphorylation is time- and dose-dependent and occurs at physiological concentrations. S-adenosylhomocysteine has no effect on protein phosphorylation but inhibits S-adenosylmethionine-dependent protein phosphorylation. ratios varying from 0 to 5 produce a dose-dependent stimulation of the phosphorylation of the 50 kDa protein. In conclusion, these results show, for the first time, that the ratio can modulate phosphorylation of a specific protein. 相似文献
19.
A V Schally W Y Huang T W Redding A Arimura D H Coy K Chihara R C Chang V Raymond F Labrie 《Biochemical and biophysical research communications》1978,82(2):582-588
A peptide found in acetic acid extracts of porcine hypothalami and capable of stimulating the release of ACTH in vitro was isolated in pure state, structurally identified as Phe-Leu-Gly-Phe-Pro-Thr-Thr-Lys-Thr-Tyr-Pre-Pro-His-Phe and synthesized. This tetradecapeptide, which corresponds to amino acid residues no. 33–46 in the sequence of the α-chain of porcine hemoglobin, probably represents an artefact of extraction or isolation procedures. Since this peptide stimulates ACTH release from rat pituitary fragments and from monolayer cultures of pituitary cells, but not , caution must be exercised in interpreting the results of assays for corticotropin releasing factor. 相似文献
20.
Akira Yanai Keijiro Kato Teruhiko Beppu Kei Arima 《Biochemical and biophysical research communications》1976,68(4):1146-1152
The mode of action of bacteriophage-induced lytic enzyme “LE95” was investigated. The LE95 hydrolyzed peptide portion in peptidoglycan of and . The exposed amino terminal amino acid was identified as glutamic acid by analysis of terminal amino acid by dinitrophenylation. This result suggested the LE95 hydrolyzed the peptide bond between L-alanine and D-glutamic acid in the peptidoglycan of and . The enzyme did not hydrolyze various peptides prepared from bacterial cell wall. This experimental result suggested that the glycan chain of peptidoglycan would be essential for the enzymic activity. 相似文献