共查询到20条相似文献,搜索用时 15 毫秒
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Elisabeth Alliot Annie Pastoureaud Joël Trellu 《Biochemical Systematics and Ecology》1980,8(4):441-445
The activities of digestive enzymes were studied during the course of larval development (from hatching to 30 days) in the sole, Solea solea. No glycosidase activity was present, but alkaline proteolytic activity increased from hatching to day 5, then decreased until day 15. In the acid range, proteolytic activity was relatively stable from the 10th to the 30th day. There were also changes in the number of esterase-like isozymes which increased between day 20 and 30, with minimum activity observed during metamorphosis. 相似文献
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Variation of intratissular carbohydrates during bud formation in root explants of Cichorium intybus cultivated in vitro .
During the cellular activation that begins with excision of root explants from Cichorium intybus L. var. Witloof cv. Zoom cultured in vitro, hydrolysis of fructose polymers, in particular of the polyfructosans (inulin) takes place. The products of degradation are used to cover the energetic needs connected with the increase of the mitotic activity. After day 2 the intracellular carbohydrates (sucrose and reducing sugars) develop differently according to further development of the explants. When growth of unorganized callus is favoured and organ formation inhibited by medium supplemented with auxin, fructose is accumulated; but under bud-forming conditions it is the amount of sucrose that increases. These differences were most notable between days 3 and 10 in culture, the period during which primordia occurred in the shoot-forming callus 相似文献
During the cellular activation that begins with excision of root explants from Cichorium intybus L. var. Witloof cv. Zoom cultured in vitro, hydrolysis of fructose polymers, in particular of the polyfructosans (inulin) takes place. The products of degradation are used to cover the energetic needs connected with the increase of the mitotic activity. After day 2 the intracellular carbohydrates (sucrose and reducing sugars) develop differently according to further development of the explants. When growth of unorganized callus is favoured and organ formation inhibited by medium supplemented with auxin, fructose is accumulated; but under bud-forming conditions it is the amount of sucrose that increases. These differences were most notable between days 3 and 10 in culture, the period during which primordia occurred in the shoot-forming callus 相似文献
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RNA synthesis in radish is studied during the first stages of germination. The radish seeds allowed to germinate in the dark, on distilled water, synthesize ribosomal RNA and accumulate a particular RNA, not incorporated in ribosomes. The results of 32P incorporation in RNA of radish seedlings indicate a progressive formation of ribosomal RNA. Two species of rapidly labelled RNA are synthesized. With labelling time, their chromatographic behaviour on MAK columus evolves, while their electrophoretic characteristics remain stable. It is assumed that these two species are involved in ribosome formation. In vivo experiments with chloramphenicol support this conclusion. RNA which accumulates during germination, could be a particular type of ribosomal RNA which could be enable, under the definite culture conditions, to enter into ribosomal structures. 相似文献
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A. Ferran P. Gubanti G. Iperti A. Migeon et J. Onillon 《Entomologia Experimentalis et Applicata》1989,53(3):229-236
Résumé Au début du printemps les adultes de Coccinella septempunctata L. (Col., Coccinellidae) issus des sites d'hivernation, les larves et les nymphes qui en proviennent, fréquentent le sol et les parties basses du blé. En juin, les adultes nés dans le champ et leur descendance sont dispersés sur toute la hauteur des talles bien qu'une fraction non négligeable de leur population se rencontre à l'extrémité supérieure. La plupart des pontes sont déposées sous les mottes de terre, les pierres et les petites adventices qui constituent des sites privilégiés. Ces variations de la distribution de cette coccinelle peuvent être mises en relation avec le climat, en particulier la température, avec la thermophilie de cette espèce et avec la répartition sur les différentes parties des talles des 3 espèces de puceron qui se succèdent au cours de la saison. Ces observations sont importantes à considérer lors du choix ou de la mise au point d'une méthode d'échantillonnage. 相似文献
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J. -C. Jeanny 《Experimental cell research》1976,102(2)
DNA and histones were studied by cytophotometry, in cartilaginous cells from urodele amphibians (Triturus vulgaris and Triturus cristatus) during ageing. With the Feulgen reaction, the DNA colorability decreased and their acid hydrolysis was more difficult. The coloration of the histones linked to DNA in chromatin was also changed. The results are discussed in relation to numerous works on deoxyribonucleoprotein modifications during cell ageing.ADN et histones ont été étudiés par cytophotométrie dans les cellules cartilagineuses d'Amphibiens Urodèles (Triturus vulgaris et Triturus cristatus) au cours du viellissement. Nous avons observé une baisse des affinités tinctoriales des ADN et une augmentation de leur résistance á l'hydrolyse acide pratiquée dans le cadre de la réaction de Feulgen. La colorabilité des histones associées aux ADN dans la chromatine est aussi modifiée. Tous ces résultats sont discutés en liaison avec les nombreux travaux effectués sur les modifications des désoxyribonucléoprotéines au cours du viellissement cellulaire. 相似文献
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B. R. Berland D. J. Bonin S. Y. Maestrini J. P. Pointier 《International Review of Hydrobiology》1972,57(6):933-944
Several methods of growth potential estimation of marine productivity are discussed: (1) simultaneous measurements of nutrient content and organic production, (2) chemical composition of algal cells, (3) bioassay with algal cultures. This last method appears to be the most convenient; it allows to study the role of a nutrient component both when utilized alone and when combined in a pool of nutrients. Several aspects of bioassay technology are discussed: test species, chemical enrichments and growth estimation of test cultures. 相似文献
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Résumé A différents temps après le début de l'infection, puis de la réinfection du cobaye par T. mentagrophytes, furent pratiqués des tests de détection de l'hypersensibilité retardée in vivo (intra dermo réaction) et in vitro (inhibition de la migration des macrophages péritonéaux) ainsi qu'un test destiné à mettre en évidence la secrétion de lymphotoxines actives vis à vis des éléments fongiques.Les résultats obtenus concernant l'évolution cinétique des réactions plaident en faveur de la plus grande sensibilité du test d'inhibition de la migration des macrophages pour la détection de l'hypersensibilité retardée. Ils confirment le rôle prépondérant des réactions de type cellulaire dans la résistance et soulignent la particularité de ce modèle expérimental oú se manifestent des phénomènes d'immunité locale. L'ensemble de nos résultats et, notamment, la démonstration de l'existence de médiateurs d'origine lymphocytaire toxiques pour les spores de T. mentagrophytes sont discutés dans le cadre de la réaction cutanée à l'agression parasitaire. 相似文献
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Phospholipid analysis of the membranes associated with fast sedimenting folded chromosomes prepared by lysis of E. coli CR 34 shows that both inner and outer membranes are parts of the complex, in proportions not very different from that found in the whole bacteria.During the preparation of the folded chromosomes, the most recently synthesized molecules of phosphatidylglycerol and phosphatidylethanoamine are more sensitive to solubilisation, particularly those from the cytoplasmic membrane. Identification of a dominant fraction, the outer membrane, in some complexes, results from a preferential solubilization of the inner membrane.These results do not favor any specific association between the folded chromosome and the membranes. 相似文献
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The presence of a nuclear DNA polymerase activity has been demonstrated in the mouse testis by a cytochemical method. The enzyme was present in the nuclei of the various cell types of mouse spermatogenesis, including spermatozoa. The nuclear activity increased during meiotic prophase and then decreased in spermatids and spermatozoa. A peculiar attention was devoted to the properties of the nuclear DNA polymerase present in spermatozoa. All four deoxyribonucleoside-triphosphates were needed for optimal activity; the acid-insoluble radioactive product obtained by polymerization in situ was completely removed by DNase, but not by RNase nor by pronase. The reaction of polymerization was DNA-dependent, sensitive to high concentrations of KCl and not affected by NEM nor by PHMB. 相似文献
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Louis-Marie Lalancette 《International Review of Hydrobiology》1987,72(2):205-223
The Comparative Productivity of Two Ponds in the Vicinity of Lake St-John, Québec In 1980 and 1981, the productivity of two ponds near Lake St. John were evaluated. One, the pond of Desbiens, did not communicate with Lake St. John and the other, the pond of St-Gédéon, was connected to the lake. The aim of this study was to evaluate the contribution that a pond could make to the increase in productivity of Lake St. John which is a reservoir. The pond of Desbiens is more productive than Lake St. John but two to three times less than the pond of St-Gédéon. The pond of Desbiens shows an advanced degree of eutrophication whereas the pond of St-Gédéon is similar to the northern lakes by its physico-chemistry, zooplankton, sediments and benthic fauna. If we want to create artificial ponds, for a better productivity of reservoirs, it is important that they be connected to each other. 相似文献
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