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RNA-mediated transposition of the tobacco retrotransposon Tnt1 in Arabidopsis thaliana. 总被引:3,自引:0,他引:3
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The tobacco (Nicotiana tabacum) retrotransposon Tnt1 was introduced into Arabidopsis thaliana. In this heterologous host plant species, Tnt1 undergoes an RNA-mediated transposition and creates a 5 bp duplication at the insertion sites. This is the first report of transposition of a retrotransposon after introduction into a heterologous host species. Tnt1 transposed during in vitro regeneration of transformed A.thaliana, but no transposition event was detected as happening in T2 and T3 generation plants. Newly synthesized copies of Tnt1 can integrate into coding regions of the host DNA. Our results open up the possibility of using Tnt1 as a new tool for insertional mutagenesis and functional analysis of plant genomes, in addition to the strategies of T-DNA and transposon tagging. 相似文献
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Regulation of expression of the tobacco Tnt1 retrotransposon in heterologous species following pathogen-related stresses 总被引:2,自引:0,他引:2
Corinne Moreau-Mhiri Jean-Benoît Morel Colette Audeon Madina Ferault Marie-Angéle Grandbastien Hélène Lucas 《The Plant journal : for cell and molecular biology》1996,9(3):409-419
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The evolutionary analysis of the Tnt1 retrotransposon in Nicotiana species reveals the high variability of its regulatory sequences 总被引:2,自引:0,他引:2
We studied the evolution of the tobacco Tnt1 retrotransposon by analyzing
Tnt1 partial sequences containing both coding domains and U3 regulatory
sequences obtained from a number of Nicotiana species. We detected three
different subfamilies of Tnt1 elements, Tnt1A, Tnt1B, and Tnt1C, that
differ completely in their U3 regions but share conserved flanking coding
and LTR regions. U3 divergence between the three subfamilies is found in
the region that contains the regulatory sequences that control the
expression of the well-characterized Tnt1-94 element. This suggests that
expression of the three Tnt1 subfamilies might be differently regulated.
The three Tnt1 subfamilies were present in the Nicotiana genome at the time
of species divergence, but have evolved independently since then in the
different genomes. Each Tnt1 subfamily seems to have conserved its ability
to transpose in a limited and different number of Nicotiana species. Our
results illustrate the high variability of Tnt1 regulatory sequences. We
propose that this high sequence variability could allow these elements to
evolve regulatory mechanisms in order to optimize their coexistence with
their host genome.
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Wilhelm M Uzun O Mules EH Gabriel A Wilhelm FX 《The Journal of biological chemistry》2001,276(50):47695-47701
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Distribution dynamics of the Tnt1 retrotransposon in tobacco 总被引:1,自引:0,他引:1
Le QH Melayah D Bonnivard E Petit M Grandbastien MA 《Molecular genetics and genomics : MGG》2007,278(6):639-651
Retrotransposons contribute significantly to the size, organization and genetic diversity of plant genomes. Although many
retrotransposon families have been reported in plants, to this day, the tobacco Tnt1 retrotransposon remains one of the few
elements for which active transposition has been shown. Demonstration that Tnt1 activation can be induced by stress has lent
support to the hypothesis that, under adverse conditions, transposition can be an important source of genetic variability.
Here, we compared the insertion site preference of a collection of newly transposed and pre-existing Tnt1 copies identified
in plants regenerated from protoplasts or tissue culture. We find that newly transposed Tnt1 copies are targeted within or
close to host gene coding sequences and that the distribution of pre-existing insertions does not vary significantly from
this trend. Therefore, in spite of their potential to disrupt neighboring genes, insertions within or near CDS are not preferentially
removed with age. Elimination of Tnt1 insertions within or near coding sequences may be relaxed due to the polyploid nature
of the tobacco genome. Tnt1 insertions within or near CDS are thus better tolerated and can putatively contribute to the diversification
of tobacco gene function.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Umetsu A Sawada Y Mitsuhashi W Mazier M Toyomasu T 《Bioscience, biotechnology, and biochemistry》2011,75(12):2398-2400
A previous study generated lettuce (Lactuca sativa) mutant lines tagged by retrotransposon Tnt1 from tobacco (Nicotiana tabacum) and identified a homozygous mutant, Tnt6a, that exhibited severe dwarf phenotype. Here we show that Tnt1 is inserted into the intron of gibberellin biosynthetic gene LsGA3ox1 in Tnt6a mutants. Expression analysis suggests that LsGA3ox1 is nearly knocked out in the Tnt6a mutants. 相似文献