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Identification of a negative regulatory region 5' of the human apolipoprotein B promoter 总被引:5,自引:0,他引:5
B Paulweber A R Brooks B P Nagy B Levy-Wilson 《The Journal of biological chemistry》1991,266(32):21956-21961
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Identification of human papillomavirus type 18 transforming genes in immortalized and primary cells. 总被引:35,自引:17,他引:18 下载免费PDF全文
The selective retention and expression of the E6-E7 region of human papillomavirus (HPV) types 16 and 18 in cervical carcinomas suggests that these viral sequences play a role in the development of genital neoplasia. Each of three possible gene products, E6, E6*, and E7, from this region of HPV-18 were examined for transforming properties in several types of rodent cells. We have found that in immortalized fibroblasts, both E6 and E7 (but not E6*) are capable of inducing anchorage-independent growth. In rat embryo cells, the HPV-18 E7 open reading frame was an effective immortalizing agent and complemented an activated ras oncogene for transformation. In both immortalized and primary cells, transformation was observed when the HPV-18 sequences were expressed from either the HPV-18 promoter or a heterologous promoter. The E6-E7 region is not, however, the sole transforming domain of HPV-18, since another portion of the early region, possibly E5, also exhibited transforming capability in immortalized fibroblasts. The development of human cervical carcinomas may therefore involve a series of steps involving multiple viral and cellular gene products. 相似文献
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