The antioxidant and peroxidase activities of saliva in patients with inflammatory periodontal diseases and possibility of their correction

The antioxidant and peroxidase activities of mixed saliva have been investigated during test loads in control group and patients with caries and combination of caries with inflammatory periodontal diseases. Antioxidant activity of saliva was significantly decreased in the last two groups and so its was corrected by means of xidiphone.     

Isolation of bacteriophages from the oral cavity

AIMS: To isolate bacteriophages lytic for oral pathogens from human saliva, dental plaque and mature biofilms constituted from saliva-derived bacteria. METHODS AND RESULTS: Saliva and dental plaque samples from healthy volunteers and from patients with gingivitis and periodontitis were examined for the presence of lytic bacteriophage using a panel of oral pathogens and bacteria isolated from the samples. Samples were also enriched for bacteriophage using static culture techniques and mature biofilms. A limited number of samples contained bacteriophage particles that were visualized using electron microscopy. Cultures yielded phage infecting non-oral bacteria (Proteus mirabilis) but no bacteriophage specific for recognized oral pathogens were found. Some micro-organisms from the oral microflora elaborated antibacterial substances that inhibited growth of other residents of the oral cavity. CONCLUSIONS: Unlike other ecosystems, the composition of the oral cavity does not appear to be heavily influenced by interactions between bacteriophages and their hosts. SIGNIFICANCE AND IMPACT OF THE STUDY: Bacteriophage for control of oral infections may need to be obtained from other sources. Antibacterial substances derived from some members of the oral microflora warrant investigation as potential antibiotics.     

Serum levels of oncostatin M (a gp 130 cytokine): an inflammatory biomarker in periodontal disease

Objective: Periodontitis is considered to be a risk factor for systemic diseases such as atherosclerosis, diabetes, etc., and cytokines play a key role. The present study was carried out to measure the level of serum oncostatin M (OSM) in patients with chronic periodontitis, and to evaluate the effect of non-surgical periodontal therapy on the serum OSM concentration.

Materials and methods: Sixty subjects were divided into three groups (each group n?=?20) based on the gingival index (GI), probing pocket depth (PPD) and clinical attachment level (CAL): group I healthy; group II gingivitis; and group III chronic periodontitis. Group III patients were followed for 8 weeks after non-surgical periodontal therapy as the after-treatment group (group IV). Estimation of serum OSM was done using an enzyme-linked immunosorbent assay.

Results: The mean OSM concentrations in serum were highest in the chronic periodontitis group (mean 68.05 pg ml^?1) and decreased following treatment (39.65 pg ml^?1) while OSM was undetectable in healthy subjects or in patients with gingivitis.

Conclusion: Increased serum OSM concentration in patients with chronic periodontitis and its positive correlation with PPD and CAL, suggest its role as an inflammatory biomarker in periodontal disease and it may exaggerate other systemic conditions such as atherosclerosis and rheumatoid arthritis.     

Proteomics for the discovery of biomarkers and diagnosis of periodontitis: a critical review

Periodontitis is a common chronic and destructive disease whose pathogenetic mechanisms remain unclear. Due to their sensitivity and global scale, proteomics studies offer the opportunity to uncover critical host and pathogen activity indicators and can elucidate clinically applicable biomarkers for improved diagnosis and treatment of the disease. This review summarizes the literature of proteomics studies on periodontitis and comprehensively discusses commonly found candidate biomarkers. Key considerations in the design of an experimental proteomics platform are also outlined. The applicability of protein biomarkers across the progression of periodontitis and unexplored areas of research are highlighted.     

Complete genome sequence of Capnocytophaga ochracea type strain (VPI 2845)

Capnocytophaga ochracea (Prévot et al. 1956) Leadbetter et al. 1982 is the type species of the genus Capnocytophaga. It is of interest because of its location in the Flavobacteriaceae, a genomically not yet charted family within the order Flavobacteriales. The species grows as fusiform to rod shaped cells which tend to form clumps and are able to move by gliding. C. ochracea is known as a capnophilic (CO(2)-requiring) organism with the ability to grow under anaerobic as well as aerobic conditions (oxygen concentration larger than 15%), here only in the presence of 5% CO(2). Strain VPI 2845(T), the type strain of the species, is portrayed in this report as a gliding, Gram-negative bacterium, originally isolated from a human oral cavity. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first completed genome sequence from the flavobacterial genus Capnocytophaga, and the 2,612,925 bp long single replicon genome with its 2193 protein-coding and 59 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.     

Predictive modeling of gingivitis severity and susceptibility via oral microbiota

Predictive modeling of human disease based on the microbiota holds great potential yet remains challenging. Here, 50 adults underwent controlled transitions from naturally occurring gingivitis, to healthy gingivae (baseline), and to experimental gingivitis (EG). In diseased plaque microbiota, 27 bacterial genera changed in relative abundance and functional genes including 33 flagellar biosynthesis-related groups were enriched. Plaque microbiota structure exhibited a continuous gradient along the first principal component, reflecting transition from healthy to diseased states, which correlated with Mazza Gingival Index. We identified two host types with distinct gingivitis sensitivity. Our proposed microbial indices of gingivitis classified host types with 74% reliability, and, when tested on another 41-member cohort, distinguished healthy from diseased individuals with 95% accuracy. Furthermore, the state of the microbiota in naturally occurring gingivitis predicted the microbiota state and severity of subsequent EG (but not the state of the microbiota during the healthy baseline period). Because the effect of disease is greater than interpersonal variation in plaque, in contrast to the gut, plaque microbiota may provide advantages in predictive modeling of oral diseases.     

Morningness–eveningness and the environment hypothesis – A cross-cultural comparison of Turkish and German adolescents

Individuals differ in morningness–eveningness, which is their preferred time for intellectual and physical activities. Although it is a basic human trait, cross-cultural comparisons are scarce but interesting because they help to determine the influence of exogenous factors, such as ambient temperature, climate and photoperiod. We here compare a large sample of German and Turkish adolescents (N?=?26?465) by using the Composite Scale of Morningness and the Morning Affect Factor. First, we show that the Morning Affect Factor and Morningness scores are equivalent in both countries and we report country differences with Turkish adolescents scoring higher on morningness (F_1,26?038?=?1293.313, p?<?0.001) and in the morning affect (F_1,26?038?=?133.833, p?<?0.001). This fits into the environment hypothesis suggesting that populations near the equator should be more morning oriented. Meanwhile, gender differences were small and adolescents showed a lower morningness and a lower morning affect with increasing age.     

国人颅骨某些角度的测量

本文对青岛和长春地区出土的124例(男71,女53)成人颅骨进行了某些角度的测量。测量结果,进行了颅骨的人类学特征和性别差异的讨论。并与其它地区的国人资料进行了比较。     

黄胸鼠的年龄鉴定和种群年龄组成

本文经晶体干重法鉴定黄胸鼠的年龄,分析其种群年龄组成,并与其他年龄鉴定方法如体重法、体长法、臼齿磨损法进行了比较。     

中国早期人类化石层位与黄土-深海沉积古气候旋回的对比

本文试从古人类化石层位在黄土剖面中的序列位置,结合黄土剖面磁性地层学研究成果,对比黄土-深海沉积古气候旋回与氧同位素期。列出中国已发现的第四纪最早的人类化石是公王岭蓝田猿人,距今年代为73—80万年。北京猿人的生活时期(北京猿人洞穴堆积1—11层)可能为距今59—12.8万年。元谋猿人,陈家窝蓝田猿人与早期北京猿人时代相当,和县猿人与晚期北京猿人时代相当,大荔人和丁村人与最后期的北京猿人时代相当。     

Age estimation by dental attrition in an independently controlled early 19th century sample from Zwolle, The Netherlands

This paper reports the investigation of macroscopic aging methods in a skeletal sample from the early 19th century, excavated in The Netherlands and containing a number of individuals of known age and sex. A method is proposed for calibrating age estimations based on dental attrition.     

Efficient determination of angles subtended by Cα-Hα and N-HN vectors in proteins via dipole-dipole cross-correlation§

The angle CH,NHN subtended by the internuclear vectors 13C-H and 15N-HN in doubly-labeled proteins can be determined by observing the effect of cross-correlation between the dipolar interactions on zero- and double-quantum coherences involving 13C and 15N. Two complementary 2D experiments with the appearance of 15N-HN correlation spectra yield signal intensities that depend on the rate of interconversion through cross-correlated relaxation of in-phase and doubly antiphase zero- and double-quantum coherences. The ratio of the signal intensities in the two experiments bears a simple relationship to the cross-correlation rate, and hence to the angle CH,NHN. Assuming planarity of the peptide bond, the dihedral angle (between C and C) can be determined from the knowledge of CH,NHN. The experiments are very time-effective and provide good sensitivity and excellent spectral resolution.     

Simultaneous determination of Ψ and Φ angles in proteins from measurements of cross-correlated relaxation effects

A method is presented to determine both and backbone angles in proteins simultaneously. This is achieved by measuring the effect on two-spin coherences of cross-correlation between ¹⁵ N-¹H^N and ¹³ vectors. The cross-correlation rates are obtained by comparing two complementary three-dimensional experiments.