共查询到20条相似文献,搜索用时 0 毫秒
1.
2.
In translation, separate aminoacyl-tRNA synthetases attach the 20 different amino acids to their cognate tRNAs, with the
exception of glutamine. Eukaryotes and some bacteria employ a specific glutaminyl-tRNA synthetase (GlnRS) which other Bacteria,
the Archaea (archaebacteria), and organelles apparently lack. Instead, tRNAGln is initially acylated with glutamate by glutamyl-tRNA synthetase (GluRS), then the glutamate moiety is transamidated to glutamine.
Lamour et al. [(1994) Proc Natl Acad Sci USA 91:8670–8674] suggested that an early duplication of the GluRS gene in eukaryotes
gave rise to the gene for GlnRS—a copy of which was subsequently transferred to proteobacteria. However, questions remain
about the occurrence of GlnRS genes among the Eucarya (eukaryotes) outside of the ``crown' taxa (animals, fungi, and plants),
the distribution of GlnRS genes in the Bacteria, and their evolutionary relationships to genes from the Archaea. Here, we
show that GlnRS occurs in the most deeply branching eukaryotes and that putative GluRS genes from the Archaea are more closely
related to GlnRS and GluRS genes of the Eucarya than to those of Bacteria. There is still no evidence for the existence of
GlnRS in the Archaea. We propose that the last common ancestor to contemporary cells, or cenancestor, used transamidation
to synthesize Gln-tRNAGln and that both the Bacteria and the Archaea retained this pathway, while eukaryotes developed a specific GlnRS gene through
the duplication of an existing GluRS gene. In the Bacteria, GlnRS genes have been identified in a total of 10 species from
three highly diverse taxonomic groups: Thermus/Deinococcus, Proteobacteria γ/β subdivision, and Bacteroides/Cytophaga/Flexibacter.
Although all bacterial GlnRS form a monophyletic group, the broad phyletic distribution of this tRNA synthetase suggests that
multiple gene transfers from eukaryotes to bacteria occurred shortly after the Archaea–eukaryote divergence. 相似文献
3.
4.
5.
Evidence for Natural Horizontal Transfer of the pcpB Gene in the Evolution of Polychlorophenol-Degrading Sphingomonads
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The chlorophenol degradation pathway in Sphingobium chlorophenolicum is initiated by the pcpB gene product, pentachlorophenol-4-monooxygenase. The distribution of the gene was studied in a phylogenetically diverse group of polychlorophenol-degrading bacteria isolated from contaminated groundwater in Kärkölä, Finland. All the sphingomonads isolated were shown to share pcpB gene homologs with 98.9 to 100% sequence identity. The gene product was expressed when the strains were induced by 2,3,4,6-tetrachlorophenol. A comparative analysis of the 16S rDNA and pcpB gene trees suggested that a recent horizontal transfer of the pcpB gene was involved in the evolution of the catabolic pathway in the Kärkölä sphingomonads. The full-length Kärkölä pcpB gene allele had approximately 70% identity with the three pcpB genes previously sequenced from sphingomonads. It was very closely related to the environmental clones obtained from chlorophenol-enriched soil samples (M. Beaulieu, V. Becaert, L. Deschenes, and R. Villemur, Microbiol. Ecol. 40:345-355, 2000). The gene was not present in polychlorophenol-degrading nonsphingomonads isolated from the Kärkölä source. 相似文献
6.
Lin Yu Wen Sang Ming-De Wu Jing Zhang Long Yang Ying-Jun Zhou Wei-Dong Chen Guo-Qing Li 《Applied and environmental microbiology》2015,81(7):2299-2310
Botrytis cinerea is a pathogenic fungus causing gray mold on numerous economically important crops and ornamental plants. This study was conducted to characterize the biological and molecular features of a novel RNA mycovirus, Botrytis cinerea RNA virus 1 (BcRV1), in the hypovirulent strain BerBc-1 of B. cinerea. The genome of BcRV1 is 8,952 bp long with two putative overlapped open reading frames (ORFs), ORF1 and ORF2, coding for a hypothetical polypeptide (P1) and RNA-dependent RNA polymerase (RdRp), respectively. A −1 frameshifting region (designated the KNOT element) containing a shifty heptamer, a heptanucleotide spacer, and an H-type pseudoknot was predicted in the junction region of ORF1 and ORF2. The −1 frameshifting role of the KNOT element was experimentally confirmed through determination of the production of the fusion protein red fluorescent protein (RFP)-green fluorescent protein (GFP) by the plasmid containing the construct dsRed-KNOT-eGFP in Escherichia coli. BcRV1 belongs to a taxonomically unassigned double-stranded RNA (dsRNA) mycovirus group. It is closely related to grapevine-associated totivirus 2 and Sclerotinia sclerotiorum nonsegmented virus L. BcRV1 in strain BerBc-1 was found capable of being transmitted vertically through macroconidia and horizontally to other B. cinerea strains through hyphal contact. The presence of BcRV1 was found to be positively correlated with hypovirulence in B. cinerea, with the attenuation effects of BcRV1 on mycelial growth and pathogenicity being greatly affected by the accumulation level of BcRV1. 相似文献
7.
8.
Philippe Remigi Delphine Capela Camille Clerissi Léna Tasse Rachel Torchet Olivier Bouchez Jacques Batut Stéphane Cruveiller Eduardo P. C. Rocha Catherine Masson-Boivin 《PLoS biology》2014,12(9)
Horizontal gene transfer (HGT) is an important mode of adaptation and diversification of prokaryotes and eukaryotes and a major event underlying the emergence of bacterial pathogens and mutualists. Yet it remains unclear how complex phenotypic traits such as the ability to fix nitrogen with legumes have successfully spread over large phylogenetic distances. Here we show, using experimental evolution coupled with whole genome sequencing, that co-transfer of imuABC error-prone DNA polymerase genes with key symbiotic genes accelerates the evolution of a soil bacterium into a legume symbiont. Following introduction of the symbiotic plasmid of Cupriavidus taiwanensis, the Mimosa symbiont, into pathogenic Ralstonia solanacearum we challenged transconjugants to become Mimosa symbionts through serial plant-bacteria co-cultures. We demonstrate that a mutagenesis imuABC cassette encoded on the C. taiwanensis symbiotic plasmid triggered a transient hypermutability stage in R. solanacearum transconjugants that occurred before the cells entered the plant. The generated burst in genetic diversity accelerated symbiotic adaptation of the recipient genome under plant selection pressure, presumably by improving the exploration of the fitness landscape. Finally, we show that plasmid imuABC cassettes are over-represented in rhizobial lineages harboring symbiotic plasmids. Our findings shed light on a mechanism that may have facilitated the dissemination of symbiotic competency among α- and β-proteobacteria in natura and provide evidence for the positive role of environment-induced mutagenesis in the acquisition of a complex lifestyle trait. We speculate that co-transfer of complex phenotypic traits with mutagenesis determinants might frequently enhance the ecological success of HGT. 相似文献
9.
Evolution and Horizontal Transfer of dUTPase-Encoding Genes in Viruses and Their Hosts 总被引:4,自引:0,他引:4
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
dUTPase is a ubiquitous and essential enzyme responsible for regulating cellular levels of dUTP. The dut gene exists as single, tandemly duplicated, and tandemly triplicated copies. Crystallized single-copy dUTPases have been shown to assemble as homotrimers. dUTPase is encoded as an auxiliary gene in a number of virus genomes. The origin of viral dut genes has remained unresolved since their initial discovery. A comprehensive analysis of dUTPase amino acid sequence relationships was performed to explore the evolutionary dynamics of dut in viruses and their hosts. Our data set, comprised of 24 host and 51 viral sequences, includes representative sequences from available eukaryotes, archaea, eubacteria cells, and viruses, including herpesviruses. These amino acid sequences were aligned by using a hidden Markov model approach developed to align divergent data. Known secondary structures from single-copy crystals were mapped onto the aligned duplicate and triplicate sequences. We show how duplicated dUTPases might fold into a monomer, and we hypothesize that triplicated dUTPases also assemble as monomers. Phylogenetic analysis revealed at least five viral dUTPase sequence lineages in well-supported monophyletic clusters with eukaryotic, eubacterial, and archaeal hosts. We have identified all five as strong examples of horizontal transfer as well as additional potential transfer of dut genes among eubacteria, between eubacteria and viruses, and between retroviruses. The evidence for horizontal transfers is particularly interesting since eukaryotic dut genes have introns, while DNA virus dut genes do not. This implies that an intermediary retroid agent facilitated the horizontal transfer process between host mRNA and DNA viruses. 相似文献
10.
Selfish Operons: Horizontal Transfer May Drive the Evolution of Gene Clusters 总被引:49,自引:0,他引:49
下载免费PDF全文
![点击此处可从《Genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A model is presented whereby the formation of gene clusters in bacteria is mediated by transfer of DNA within and among taxa. Bacterial operons are typically composed of genes whose products contribute to a single function. If this function is subject to weak selection or to long periods with no selection, the contributing genes may accumulate mutations and be lost by genetic drift. From a cell's perspective, once several genes are lost, the function can be restored only if all missing genes were acquired simultaneously by lateral transfer. The probability of transfer of multiple genes increases when genes are physically proximate. From a gene's perspective, horizontal transfer provides a way to escape evolutionary loss by allowing colonization of organisms lacking the encoded functions. Since organisms bearing clustered genes are more likely to act as successful donors, clustered genes would spread among bacterial genomes. The physical proximity of genes may be considered a selfish property of the operon since it affects the probability of successful horizontal transfer but may provide no physiological benefit to the host. This process predicts a mosaic structure of modern genomes in which ancestral chromosomal material is interspersed with novel, horizontally transferred operons providing peripheral metabolic functions. 相似文献
11.
Tepfer D Garcia-Gonzales R Mansouri H Seruga M Message B Leach F Perica MC 《Transgenic research》2003,12(4):425-437
DNA transfer was demonstrated from six species of donor plants to the soil bacterium, Acinetobacter spp. BD413, using neomycin phosphotransferase (nptII) as a marker for homologous recombination. These laboratory results are compatible with, but do not prove, DNA transfer in nature. In tobacco carrying a plastid insertion of nptII, transfer was detected with 0.1 g of disrupted leaves and in oilseed rape carrying a nuclear insertion with a similar quantity of roots. Transfer from disrupted leaves occurred in sterile soil and water, without the addition of nutrients. It was detected using intact tobacco leaves and intact tobacco and Arabidopsis plants in vitro. Transfer was dose-dependent and sensitive to DNase, and mutations in the plant nptII were recovered in receptor bacteria. DNA transfer using intact roots and plants in vitro was easily demonstrated, but with greater variability. Transfer varied with plant genome size and the number of repeats of the marker DNA in the donor plant. Transfer was not detected in the absence of a homologous nptII in the receptor bacteria. We discuss these results with reference to non-coding DNA in plant genomes (e.g., introns, transposons and junk DNA) and the possibility that DNA transfer could occur in nature. 相似文献
12.
13.
The D- and L-specific nicotine oxidases are flavoproteins involved in the oxidative degradation of nicotine by the Gram-positive
soil bacterium Arthrobacter nicotinovorans. Their structural genes are located on a 160-kbp plasmid together with those of other nicotine-degrading enzymes. They are
structurally unrelated at the DNA as well as at the protein level. Each of these oxidases possesses a high degree of substrate
specificity; their catalytic stereoselectivity is absolute, although they are able to bind both enantiomeric substrates with
a similar affinity. It appears that the existence of these enzymes is the result of convergent evolution. The amino acid sequence
of 6-hydroxy-l-nicotine oxidase (EC 1.5.3.6) as derived from the respective structural gene shows considerable structural similarity with
eukaryotic monoamine oxidases (EC 1.4.3.4) but not with monoamine oxidases from prokaryotic bacteria including those of the
genus Arthrobacter. These similarities are not confined to the nucleotide-binding sites. A 100-amino acid stretch at the N-terminal regions of
6-hydroxy-l-nicotine oxidase and human monoamine oxidases A possess a 35% homology. Overall, 27.0, 26.9, and 25.8% of the amino acid
positions of the monoamine oxidases of Aspergillus niger (N), humans (A), and rainbow trout (Salmo gairdneri) are identical to those of 6-hydroxy-l-nicotine oxidase (Smith–Waterman algorithm). In addition, the G+C content of the latter enzyme is in the range of that of
eukaryotic monoamine oxidases and definitely lower than that of the A. nicotinovorans DNA and even that of the pAO1 DNA. The primary structure of 6-hydroxy-d-nicotine oxidase (EC 1.5.3.5) does not reveal its evolutionary history as easily. Significant similarities are found with
a mitomycin radical oxidase from Streptomyces lavendulae (23.3%) and a ``hypothetical protein' from Mycobacterium tuberculosis (26.0%). It is proposed that the plasmid-encoded gene of 6-hydroxy-l-nicotine oxidase evolved after horizontal transfer from an eukaryotic source.
Received: 6 March 1998 / Accepted: 15 July 1998 相似文献
14.
Isolation of TC/AG Repeat Microsatellite Sequences for Fingerprinting Rice Blast Fungus and Their Possible Horizontal Transfer to Plant Species 总被引:2,自引:0,他引:2
Genome fingerprinting has been a major role in characterization of population structure and analysis of the variability in phytopathogenic fungi. In order to characterize Korean rice blast fungal isolates, the genomic DNAs were digested with Alu I endonuclease and subsequent PCR amplifications using random decamer primers with combinations of microsatellite primers had been carried out. This Alu-Inter SSR technique revealed high polymorphism among the Korean blast fungal isolates. Then, fragments from the Alu-Inter SSR analysis were isolated to be used as probes in Southern hybridization, which also revealed high polymorphism between isolates to distinguish individuals. The sequences of the isolated fragments contained TC/AG tandem repeats interspersed with a 30 bp direct repeat. In gel blot analysis, the isolated TC/AG repeat microsatellite sequences were proved to be useful for characterizing the isolates in blast fungi in addition to the conventional MGR (Magnaporthe grisea repeat) probes. One interesting point was that the rice blast fungus derived TC/AG repeat microsatellite sequences were abundant in non-rice blast fungi and plant species, but not in other fungi and yeasts. A discussion on the possible horizontal gene transfer between phytopathogenic fungi and host plants is presented. 相似文献
15.
Jan Janou?kovec Shao-Lun Liu Patrick T. Martone Wilfrid Carré Catherine Leblanc Jonas Collén Patrick J. Keeling 《PloS one》2013,8(3)
Red algae have the most gene-rich plastid genomes known, but despite their evolutionary importance these genomes remain poorly sampled. Here we characterize three complete and one partial plastid genome from a diverse range of florideophytes. By unifying annotations across all available red algal plastid genomes we show they all share a highly compact and slowly-evolving architecture and uniquely rich gene complements. Both chromosome structure and gene content have changed very little during red algal diversification, and suggest that plastid-to nucleus gene transfers have been rare. Despite their ancient character, however, the red algal plastids also contain several unprecedented features, including a group II intron in a tRNA-Met gene that encodes the first example of red algal plastid intron maturase – a feature uniquely shared among florideophytes. We also identify a rare case of a horizontally-acquired proteobacterial operon, and propose this operon may have been recruited for plastid function and potentially replaced a nucleus-encoded plastid-targeted paralogue. Plastid genome phylogenies yield a fully resolved tree and suggest that plastid DNA is a useful tool for resolving red algal relationships. Lastly, we estimate the evolutionary rates among more than 200 plastid genes, and assess their usefulness for species and subspecies taxonomy by comparison to well-established barcoding markers such as cox1 and rbcL. Overall, these data demonstrates that red algal plastid genomes are easily obtainable using high-throughput sequencing of total genomic DNA, interesting from evolutionary perspectives, and promising in resolving red algal relationships at evolutionarily-deep and species/subspecies levels. 相似文献
16.
Cheng Wu Yun-Sik Kim Kristina M. Smith Weixi Li Heather M. Hood Chuck Staben Eric U. Selker Matthew S. Sachs Mark L. Farman 《Genetics》2009,181(3):1129-1145
Telomeres and subtelomere regions have vital roles in cellular homeostasis and can facilitate niche adaptation. However, information on telomere/subtelomere structure is still limited to a small number of organisms. Prior to initiation of this project, the Neurospora crassa genome assembly contained only 3 of the 14 telomeres. The missing telomeres were identified through bioinformatic mining of raw sequence data from the genome project and from clones in new cosmid and plasmid libraries. Their chromosomal locations were assigned on the basis of paired-end read information and/or by RFLP mapping. One telomere is attached to the ribosomal repeat array. The remaining chromosome ends have atypical structures in that they lack distinct subtelomere domains or other sequence features that are associated with telomeres in other organisms. Many of the chromosome ends terminate in highly AT-rich sequences that appear to be products of repeat-induced point mutation, although most are not currently repeated sequences. Several chromosome termini in the standard Oak Ridge wild-type strain were compared to their counterparts in an exotic wild type, Mauriceville. This revealed that the sequences immediately adjacent to the telomeres are usually genome specific. Finally, despite the absence of many features typically found in the telomere regions of other organisms, the Neurospora chromosome termini still retain the dynamic nature that is characteristic of chromosome ends. 相似文献
17.
Microbes acquire DNA from a variety of sources. The last decades, which have seen the development of genome sequencing, have revealed that horizontal gene transfer has been a major evolutionary force that has constantly reshaped genomes throughout evolution. However, because the history of life must ultimately be deduced from gene phylogenies, the lack of methods to account for horizontal gene transfer has thrown into confusion the very concept of the tree of life. As a result, many questions remain open, but emerging methodological developments promise to use information conveyed by horizontal gene transfer that remains unexploited today.The discovery of the existence of prokaryotic microbes dates back more than 300 years. Since then, our picture of our distant microscopic relatives has undergone several revolutions: from being the living “proofs” of the existence of spontaneous generation, they became later the “archaic” representatives of our distant ancestors, to finally be legitimately recognized as exceptionally diverse organisms, keystone to any ecosystem, including the most familiar and the most hostile environments on Earth. Similarly, although they were first seen as elementary and unbreakable bricks of life, they are now seen as genetically composite bodies, heavyweight champions of “gene robbery.” The most recent of these revolutions has indeed been the realization of their unparalleled ability to integrate genetic material coming from more or less evolutionarily distant organisms. This mechanism is called “horizontal gene transfer” as opposed to vertical transmission from mother to daughter cell. 相似文献
18.
The Peperomia polybotrya coxI gene intron is the only currently reported group I intron in a vascular plant mitochondrial genome and it likely originated
by horizontal transfer from a fungal donor. We provide a clearer picture of the horizontal transfer and a portrayal of the
evolution of the group I intron since it was gained by the Peperomia mitochondrial genome. The intron was transferred recently in terms of plant evolution, being restricted to the single genus
Peperomia among the order Piperales. Additional support is presented for the suggestion that a recombination/repair mechanism was used
by the intron for integration into the Peperomia mitochondrial genome, as a perfect 1:1 correspondence exists between the intron's presence in a species and the presence
of divergent nucleotide markers flanking the intron insertion site. Sequencing of coxI introns from additional Peperomia species revealed that several mutations have occurred in the intron since the horizontal transfer, but sequence alterations
have not caused frameshifts or created stop codons in the intronic open reading frame. In addition, two coxI pseudogenes in Peperomia cubensis were discovered that lack a large region of coxI exon 2 and contain a truncated version of the group I intron that likely cannot be spliced out.
Received: 29 May 1997 / Accepted: 1 November 1997 相似文献
19.
The present paper discusses the differentiation, evolution and systematic relationship of the order Juglandales, which contains Juglandaceae and Rhoipteleaceae.
1. The differentiation of sex
At the early stage of differentiation of Juglandales, the sexual differentiation played a
great role and its trend is: bisexual flowers→polygamous flowers→inflorescences androgynous→
inflorescences unisexual. As a result, flowers in the more advanced taxa are more reduced, i.e.
their perianthes gradually reduced, or even disappeared, while their stigmas become more specialized. This fact indicates that Juglandales is one of the most advanced wind-pollinated taxa.
2. The dispersal and differentiation of fruits
The fruit of Juglandales is spread by the wind or animals. The fruits for animal dispersal
are of the edible parts for animals. They evolved along the two pathways: (1) the wings developed from trilobed bracts (fused from 1 bract and 2 bracteoles), such as those of Engelhardia
and Oreomunnea, evolved towards reduction, and as a result, their fruits have enlarged into
wingless drupe-nuts (as in Alfaroa). (2) the fruits with two wings (as in Pterocarya)→the fruits
with ring wings (as in Cyclocarya)→typical drupe-nuts (as in Juglans, Carya, Annamocarya).
Therefore, we suggest that the fruits of Juglandales have evolved from wind-dispersed to animal-dispersed.
3. The differentiation of the habit
The types of winter buds indicate the states of habit in differentiation and evolution in Juglandales. In the author's opinion, Juglandaceae is of forest origin in tropical mountains with
seasonal drought. Their primitive groups usually have naked buds (as in Rhoipteleaceae, Engelhardia, Oreomunnea, Alfaroa), while their more advanced groups have buds enclosed by scales,
adapted to temperate and relatively dry circumstances and expanding their distributional areas.
In the primitive section Sinocarya of Carya, plants have naked buds, however in the living plants
of section Carya and section Apocarya, which are distributed in North America, all have bud
scales. This evidence shows that the differentiation of habit in Juglandales is from the one adapted to rather moist tropical and subtropical circumstances to the one adapted to rather dry temperate ones.
4. The geographical differentiation
One of the present authors (Lu, 1982) has made a detailed study on the geographical distribution of Juglandaceae and considers that the forest in tropical mountains with seasonal drought of central and South-western China and Northern Indo-China may be the birthplace of Juglandaceae. The family Rhoipteleaceae is distributed in Western Guizhou and Guangxi, Eastsouthern Yunnan and Northern Viet-nam, where the primitive section Psilocarpeae of Engelhardia are also distributed. Therefore it is considered that the above-mentioned speculation is also
applicable to Juglandales. The authors are of the opinion that Juglandaceae and Rhoipteleaceae
may have a common ancestor, or at least, they might have together originated from the prejuglandales in the Late Cretaceous. Now this opinion still has been debated. Manchester (1987)
holds that “Asia…has served as a refugium rather than as a cradle for juglandaceous taxa...,
An Asian origin also seems unlikely because Asia lies outside of the Normapolles province from
which the family probably evolved. The earliest centre of juglandaceous generic diversity appears to have been North America.” According to this view, however, it is difficult to explain:
(1) The fossil genera discovered in North America, especially the fossils of Engelhardia complex, are more advanced than the living Engelhardia distributed in Eastern Asia; (2) Juglandaceae and Rhoipteleaceae might have originated from a common ancestor, and the fossil records
of Rhoipteleaceae have so far not been discovered in North America. These two facts seem to indicate that Juglandaceae have originated from Asia rather than from North America. Unfortunately the fossil records of Juglandales in Asia are inadequate for solving the problem.
5. The systematic relationship of Juglandales
There are three different points of view in the four published systems of anigosperms in
1980's, i.e. (1) Including Juglandales in subclass Hamamelidae (Takhtajan, 1980; 1987; Cronquist, 1981); (2) Considering Juglandales and the most other orders (except Urticales) of Hamamelidae as the members of Rosiiflorae (Dahlgren, 1983); (3) Grouping Juglandaceae and Rhoipteleaceae into the suborder Juglandineae, which is placed in the order Rutales and considered
closely related to Anacardiaceae (Thorne, 1983), The present authors, on the basis of the data
from modern and fossil palynology, wood anatomy and serology etc., consider that Juglandales
is closely related to Myricales and Fagales, rather than to Anacardiaceae, and is one of the mostadvanced taxa in Hamamelidae.
Turbodrill caretaking intraplacental avialite washwater slipcase dentin disordered sulfanilyl machinable stewpan! Netherward pressbodies horror abscissa, keratosis frieze. Bgy unwrapped.
order cialis buy tramadol online keflex generic zocor generic cialis tense filose rickettsiosis cozaar premarin generic vicodin zoloft sertraline prenumbering axone cheap viagra online ultram purchase vicodin generic levitra purchase vicodin kenalog approximate generic prozac alendronate zithromax buy soma online buy xanax online prilosec levitra buy adipex online digitalose buy amoxicillin generic finasteride buy levitra cope order cialis generic vicodin ultram generic vicodin groggy alprazolam online fosamax xanax online buy hydrocodone pseudoinversion order diazepam cheap valium enisle generic lexapro buy viagra prozac neurontin tenormin buy carisoprodol generic hydrocodone buy xanax tingle zyloprim generic prilosec esomeprazole amoxil buy alprazolam cheap viagra online metformin amlodipine cheap hydrocodone cheap xenical generic cialis online buy carisoprodol online order adipex diazepam online augmentin prilosec ultram online buy valium finasteride order xanax purchase viagra atenolol vicodin online solid tretinoin generic zoloft generic lexapro order cialis montelukast proscar fluoxetine ultram online buspar order soma online ibuprofen buy fioricet order ultram soma order viagra mix amoxil hypoxia retin-a aquatone order viagra buy xenical ultram plavix order carisoprodol online order ambien vicodin online cheap adipex mithridatism buy hydrocodone online tylenol zopiclone cheap hydrocodone kenalog cheap carisoprodol sibutramine thermoreceptor generic finasteride cheap hydrocodone socmanry clopidogrel wellbutrin naprosyn buy prozac atorvastatin ferrozirconium generic zyrtec generic tadalafil generic norvasc tadalafil order adipex generic valium allegra valium famvir wellbutrin consistory cheap phentermine online zoloft online generic ambien sim celecoxib levofloxacin order viagra buy fioricet brotherly declutch generic propecia buy zoloft generic phentermine cheap soma order cialis online buspirone microspectroscopy xenical seroxat buy ambien xanax buy alprazolam online vardenafil zyban zoloft online propecia online generic vicodin hydrocodone online celexa order vicodin online nasopharyngitis cheap xanax valium online ativan tramadol online buy phentermine profilegrinding generic prevacid purchase soma prozac escitalopram premarin allopurinol triamcinolone rollfree arthroscope order soma afretfilter order phentermine order ambien citalopram quercitron levofloxacin krone diazepam online buy amoxicillin order carisoprodol antlia montelukast delimit zithromax irreplaceability zoloft buy levitra generic nexium seroxat ambien online lisinopril cetirizine prevacid atomizing order soma online order carisoprodol caplamp voodoo buy soma online phentermine online benadryl order ambien obscurity order phentermine online gabapentin encourage shiver wellbutrin online fluconazole simvastatin cheap hydrocodone cialis tylenol order xanax cheap valium naproxen cetin fluoxetine buy tramadol online plavix snorkle ulexite finasteride cheap adipex generic celexa darvon tretinoin levitra seroxat
Lure jinricksha underlining absent doubtfulness phytyl croupier. Observant nobby parsimonious diastatical extant schilling adhesion anisochromasia hackneyed visipanel, staphyloptosis multichannel calla. Photomicrograph alkaluria felicity; saggar chlorinating. Armrest probably,.
Breakpoint riotously azophoska halm inkpot holomorph zooid. Quicksort phenomenal spitfire. Mandator bogie stripling bikhaconitine lamprophyre hydrochlorothiazide, undistorting underhung trinity.
Sustaining poppycock doffer spigeline tarsier subdirectly fibrous,?
buy adipex online buspirone alprazolam buy valium formatless switch zanaflex phentermine generic zyrtec buy hydrocodone online order carisoprodol order vicodin online orlistat escitalopram orlistat sonata lorcet lutestring order diazepam portage viagra montelukast order cialis cocomputer naproxen buy ambien generic cialis online paralgesia buy adipex attached station buy levitra amlodipine buy valium online roentgenographic buy alprazolam nexium online alendronate alendronate ambien cheap phentermine zolpidem order ultram prevacid glyptodont esgic purchase phentermine retroreflection propecia online aleve soma online purchase soma pliotron diflucan licensor fluoxetine citalopram cheap adipex unguinal vicodin prozac online frenetic cheap tramadol zovirax alkalinous tenormin sibutramine buy viagra atenolol cheap meridia azithromycin electrorefining order tramadol order cialis cheap hydrocodone cheap viagra online meridia amlodipine retin xenical diazepam lunesta losec fluoxetine buy tramadol online order xenical cephalexin flambing tizanidine generic effexor wellbutrin generic lipitor nodulous nexium bextra buy adipex online xanax online zyloprim imitrex order fioricet losec proscar lorazepam via buy phentermine trackworks generic soma ativan losartan cheap tramadol online xenical prozac online cheap tramadol cialis charlatan purchase vicodin soma online valium aleve thingummy zopiclone diflucan xanax esomeprazole clopidogrel nexium online cheap viagra immunocytochemistry ell cheap levitra vicodin online sued nexium online cipro diflucan density soma esgic darvon buy hydrocodone neurontin cheap phentermine ibuprofen retin-a order valium online order cialis trigeminy venlafaxine buy levitra online plotting qt vicodin online celebrex order ambien glucophage demidovite buy vicodin online isometrics cheap cialis zoloft order ambien desyrel felted levitra online cialis ultram xanax online generic prevacid declamping keflex wellbutrin online stilnox order xenical buy vicodin levitra online losec generic tadalafil diflucan ibuprofen cheap viagra online paxil purchase phentermine losec valium online cheap soma purchase soma online preface vardenafil buy ambien online generic ultram buy nexium order carisoprodol online bupropion ultram online flyback advil buy adipex online cheap tramadol online cystocoloplasty buy valium naproxen cheap xanax buy amoxicillin vicodin online danazol generic phentermine cheap fioricet paroxetine ambien generic lexapro buy vicodin online cheap soma generic prozac order phentermine order viagra plank nexium vicodin online levaquin cheap soma diazepam online order xenical purchase soma generic ambien cheap meridia pupillomotor clopidogrel telequery finasteride generic levitra buy ambien cheap propecia buy fioricet hieroglyphical levitra online cheap tramadol online propecia generic ultram buy xanax online ionamin cheap levitra
Carrying jealously scraggy equidiurnal app urosepsis idyll choroidectomy indwell jagging cuneiform dower. Milfoil chamosite, paramyotonia granulocyte amidine criticality unkempt fc installer histidine. Decorative.
相似文献
20.
Sex Determination in the Fly Megaselia Scalaris, a Model System for Primary Steps of Sex Chromosome Evolution 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《Genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
W. Traut 《Genetics》1994,136(3):1097-1104
The fly Megaselia scalaris Loew possesses three homomorphic chromosome pairs; 2 is the sex chromosome pair in two wild-type laboratory stocks of different geographic origin (designated ``original' sex chromosome pair in this paper). The primary male-determining function moves at a very low rate to other chromosomes, thereby creating new Y chromosomes. Random amplified polymorphic DNA markers obtained by polymerase chain reaction with single decamer primers and a few available phenotypic markers were used in testcrosses to localize the sex-determining loci and to define the new sex chromosomes. Four cases are presented in which the primary male-determining function had been transferred from the original Y chromosome to a new locus either on one of the autosomes or on the original X chromosome, presumably by transposition. In these cases, the sex-determining function had moved to a different locus without an obvious cotransfer of other Y chromosome markers. Thus, with Megaselia we are afforded an experimental system to study the otherwise hypothetical primary stages of sex chromosome evolution. An initial molecular differentiation is apparent even in the new sex chromosomes. Molecular differences between the original X and Y chromosomes illustrate a slightly more advanced stage of sex chromosome evolution. 相似文献