Phillips Dikdiks (Madoqua saltiana phillipsi) in der Al Wabra Preservation (AWWP)/Katar

Since the 1990s Phillips dikdiks (Madoqua saltiana phillipsi) are kept and successfully bred at the Al Wabra Wildlife Preservation (AWWP) in Qatar. This paper presents the observations on the social behaviour and the biology of this subspecies of the Salt's dikdik, originated at the Somali gulf coast. Husbandry, nutrition and handling of this species are explained as well as the demography of the AWWP population, diseases and death causes. AWWP is so far the only known institution which keeps Phillips dikdiks.     

Haltung und Zucht des Spix-Aras (Cyanopsitta spixii) in der Loro Parque Fundación auf Teneriffa

Since 2004 the Loro Parque Fundación (LPF) has again achieved breeding success with the Spix's Macaw (Cyanopsitta spixii), and has been regularly rearing young (n=4) by hand-feeding. Currently this parrot species, which went extinct in the wild in 2000, cannot be seen on exhibit in any zoo in the world. There are only five breeding centres worldwide which keep this species, all being members of the Spix's Macaw Recovery Programme. The conditions and techniques used by the LPF to maintain, breed and hand-rear this species are described.     

Pansenfehlgärung und Lösungsansätze bei der Handaufzucht einer verwaisten Beira-Antilope (Dorcatragus megalotis)

Within the last 11 years several Beira antelope fawns (Dorcatragus megalotis) had been hand reared for several reasons at Al Wabra Wildlife Preservation (AWWP) in Qatar (Abb. 1). Hand rearing Beira antelopes is usually not more difficult than hand rearing any other small antelope species as long as no complications occur. The following case report describes the problems that occurred while artificially rearing an orphaned Beira fawn as well as our approach to a solution.     

Body size development of captive and free‐ranging Leopard tortoises (Geochelone pardalis)

The growth and weight development of Leopard tortoise hatchings (Geochelone pardalis) kept at the Al Wabra Wildlife Preservation (AWWP), Qatar, was observed for more than four years, and compared to data in literature for free‐ranging animals on body weight or carapace measurements. The results document a distinctively faster growth in the captive animals. Indications for the same phenomenon in other tortoise species (Galapagos giant tortoises, G. nigra; Spur‐thighed tortoises, Testudo graeca; Desert tortoises, Gopherus agassizi) were found in the literature. The cause of the high growth rate most likely is the constant provision with highly digestible food of low fiber content. Increased growth rates are suspected to have negative consequences such as obesity, high mortality, gastrointestinal illnesses, renal diseases, “pyramiding,” fibrous osteodystrophy or metabolic bone disease. The apparently widespread occurrence of high growth rates in intensively managed tortoises underlines how easily ectothermic animals can be oversupplemented with nutrients. Zoo Biol 29:517–525, 2010. © 2009 Wiley‐Liss, Inc.     

Reducing the conservation reliance of the endangered Kirtland's warbler through adaptive management

Species are considered conservation-reliant when their continued existence is dependent on human assistance. Conservation reliance challenges the conservation community in terms of their ability to sustain the funding and public-private partnerships needed for indefinite management. Although increased funding for conservation is critical, reducing conservation reliance through adaptive management represents an attractive alternative. We used a large-scale ecological experiment as a case study in the use of adaptive management to reduce conservation reliance. For >40 years, the United States Fish and Wildlife Service has trapped and lethally removed an obligate brood parasite, the brown-headed cowbird (Molothrus ater), to protect the Kirtland's warbler (Setophaga kirtlandii) from the negative effects that brood parasitism has on its reproductive success. To determine if the conservation reliance of the Kirtland's warbler could be reduced through optimization of the cowbird control program, we used an adaptive management approach. In collaboration with stakeholders, we experimentally reduced cowbird trapping effort across nearly all of the Kirtland's warbler breeding range. We monitored the resulting cowbird abundance and rate of parasitism, and then adjusted the scale of trap reductions based on the previous year's results. Despite reducing (2015–2017) and eventually eliminating (2018) cowbird trapping, we detected only 20 cowbirds (2015–2017) and found that just 4 of 514 (<1%) nests were parasitized (2015–2018). Our results indicate that the cowbird control program can at least temporarily be suspended, thereby reducing conservation reliance in the Kirtland's warbler and freeing funds for other management. We urge the conservation community to consider the use of adaptive management to reduce conservation reliance in other threatened and endangered taxa. © 2019 The Wildlife Society.     

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

The aim of the present study was to characterize the epididymal sperm of Spix's yellow‐toothed cavy (Galea spixii) through two different recovery methods. Nine sexually mature males were euthanized and the complexes, testes–epididymis, were dissected. For each animal, one epididymis was processed by flotation method and the other was processed by retrograde flushing method, both using a TES‐based buffered media. After recovery, we evaluated the sperm for motility, vigour, viability, functional membrane integrity and morphology. Morphometric data from the different sperm regions were evaluated by using an appropriate software. After recovery, both methods provide similar values for all the sperm parameters, aiming the recovery of more than 300 × 10⁶ sperm, presenting >50% motile sperm, with normal morphology and functional membrane. The total sperm length in this sperm was 48.87 ± 0.1, and the sperm head presented 9.4%, on average. A notable characteristic was the prominent acrosome found in the G. spixii sperm. In conclusion, we demonstrate that either flotation or retrograde flushing methods are suitable for the recovery of sperm from cauda epididymis of Spix's yellow‐toothed cavies.     

Parentage verification of a mountain zebra (Equus zebra hartmannae) using polymorphic microsatellite markers

The Hartmann's mountain zebra (Equus zebra hartmannae) has been classified by the IUCN as a vulnerable species. Approximately 300 individuals, maintained in zoos throughout Europe and the United States of America, are being managed as part of a captive breeding program. An International Studbook is maintained for the Hartmann's mountain zebra at Marwell Zoological Park, UK. Despite the use of a variety of means to identify each individual in a captive herd, confusion sometimes occurs, resulting in the misidentification of an animal. Here we report the first application of DNA typing, using polymorphic microsatellite loci, to resolve a misidentification involving two female Hartmann's mountain zebra. This case demonstrates the way in which genetic tests derived from a related domesticated species may be used as an effective tool for captive management. Further, this case highlights the need to be able to conclusively identify captive individuals and to maintain accurate pedigree information for successful captive management. © 1995 Wiley-Liss, Inc.     

Factors influencing breeding success,ovarian cyclicity,and cub survival in zoo-managed tigers (Panthera tigris)

Understanding factors that influence reproduction and offspring survival in zoo populations is critical for management of threatened and endangered species. Examination of long-term data (1989–2011) compiled from the Association of Zoos and Aquarium's zoo-managed tiger breeding program provides the basis for a more thorough understanding of reproduction and scientifically based decisions for effective population management in this endangered felid. Biological and management-related factors that could influence tiger breeding success and cub survival were evaluated using logistic mixed models. Breeding success improved with female age until approximately age five, then declined thereafter. Experienced female breeders had greater breeding success than inexperienced females. Litter size was most predictive of cub survival, with average-sized litters (3–4 cubs) experiencing the highest proportional survival. Management-related factors, such as whether the breeding institution had a recent tiger litter and whether both animals were already located at the same institution, also influenced breeding success and cub survival. These results highlight the importance of institutional husbandry experience and the need to retain knowledge through staff turnovers to achieve optimal reproductive success. Using fecal estrogen data, frequency of ovarian cyclicity and mean cycle length did not differ by female age or parity; thus, lack of cyclicity and/or increased cycle duration are not likely explanations for declining breeding success with age. These results provide valuable reproductive information that should improve scientific management of zoo-based tiger populations.     

Pedigree analysis for the genetic management of group‐living species

Captive breeding programs are an important tool for the conservation of endangered species. These programs are commonly managed using pedigrees containing information about the history of each individual's family, such as breeding pairs and parentage. However, there are some species that are kept in groups where it is hard to distinguish between particular individuals within the group, making it very difficult to record any information at an individual level. Currently, software and methods commonly used for registering and analyzing pedigrees to help manage populations at an individual level are not adequate for managing these group‐living species. Therefore, there is a need to further develop these tools and methodologies for pedigree analysis to better manage group‐living species. PMx is a program used for the management of ex situ populations in zoos and aquariums. We adapted the pedigree analysis method implemented in PMx to analyze pedigrees (records of descendant lineages) of group‐living species. In addition, we developed a group pedigree data entry sheet and group2PMx, a converter program that enables group datasets to be imported into PMx. We show how pedigree analysis of a group‐living species can be used for population management using the studbook of the endangered Texas blind cave salamander Eurycea rathbuni. Such analyses of the pedigree of groups can improve the management of group‐living species in ex situ breeding programs. Firstly, it enables better management decisions based on more accurate genetic measures between groups, allowing for greater control of inbreeding. Secondly, it can improve the conditions in which group‐living species are held by adapting husbandry practices to better reflect conditions of these species living in the wild. The use of the spreadsheet and group2PMx extends the application of PMx_, allowing conservation managers and other institutions outside the zoo and aquarium community to easily import and analyze their pedigree data.     

福建省自然保护地管理有效性评估——基于保护地快速评估和优先性确定方法

在自然保护地管理体制改革之际,对保护地开展管理有效性评价可以识别保护地面临的威胁与压力,确定保护地管理工作的优先性。研究以保护地快速评估和优先性确定方法(Rapid Assessment and Prioritization of Protected Areas Management, RAPPAM)为基础,结合福建省省情优化管理评价指标体系,对福建省9个地级市的53处自然保护地开展管理有效性评估,识别自然保护地面临的主要压力与威胁,分析保护地管理上的优劣势,并选用斯皮尔曼相关性分析,识别有利于减轻保护地威胁的相关管理环节,为福建省保护地管理工作的优先性调整提供参考。结果表明：(1)福建省自然保护地面临的主要威胁和压力依次为火灾、种养殖、虫害、环境污染和旅游,其中,环境污染与旅游对保护地的影响严重。(2)福建省自然保护地体系管理水平总体一般,存在地域性差异。自然保护地普遍存在多头管理、管理投入不足、管理实施计划缺失等问题。(3)由斯皮尔曼关联性分析可知,保护地管理过程与投入的提升有助于降低火灾、虫害、种养殖的威胁。环境污染和旅游威胁受宏观经济发展、保护地及周边用地规划影响显著。     

Between global citizenship and Qatarization: negotiating Qatar's new knowledge economy within American branch campuses

Over the last decade, the Gulf state of Qatar has invested billions of dollars in American branch campuses as part of its development as a ‘knowledge-based economy’. A knowledge economy will allow Qatar to diversify from petroleum wealth and reduce the country's reliance on foreign labour by introducing more citizens into the workforce, a process called ‘Qatarization’. While intended to bolster nativism, branch campuses are organized around certain Western liberal norms, such as meritocracy, egalitarianism and multiculturalism. These manifest in several ways, including English education, gender integration and a student body that is composed of more non-citizens than Qatari nationals. In this article, I explore how non-citizen students in particular, many of who were born and raised in Qatar, interact with Qatar's new knowledge economy, paying particular attention to the seemingly contradictory models of ‘global citizenship’ on the one hand and ‘Qatarization’ on the other – one a philosophy that is open and inclusive, and the other specifically closed and exclusive.     

Elimination of inbreeding depression from a captive population of Speke's gazelle: Validity of the original statistical analysis and confirmation by permutation testing

The Speke's gazelle captive breeding program was designed in the early 1980s to simultaneously maintain the population's genetic diversity while reducing the severity of the inbreeding depression in a situation in which inbreeding could not be avoided. Statistical analyses of the resulting data using both regression techniques and nonparametric exact contingency tests revealed that the inbreeding depression was indeed reduced, and genetic surveys revealed that high levels of nuclear genetic diversity had indeed been maintained. Hence, the twin goals of the breeding program appeared to have been achieved. Recently, several papers have been published that question the validity of the original statistical analyses and resulting biological conclusions. Specifically, these papers raise three major issues: (1) that a small sample correction factor used in the regression analysis represents a statistical “flaw,” (2) that new analyses of the data do not confirm the original conclusion of a significant reduction in the level of inbreeding depression, and (3) that the biological conclusions about the program are not justified. In this paper we show (1) that there is no “flaw” in the small sample correction, (2) that the recent permutational test given by Willis and Wiese seriously violates standard procedures and has no statistical validity, (3) that the regression procedures used by Ballou are inappropriate because the data seriously violate the underlying statistical assumptions and that the statistically valid components of Ballou's work strongly confirm the validity of the Speke's gazelle program, (4) that permutational tests done in accordance with standard statistical practice strongly confirm the results of the original analysis, and (5) that the original biological conclusions are fully justified by multiple types of statistical analyses. Zoo Biol 17:77–94, 1998. © 1998 Wiley-Liss, Inc.     

Survey of the long-tailed macaques introduced onto Tinjil Island,Indonesia

From February 1988 though June 1990, 475 adult long-tailed macaques (Macaca fascicularis) were released on Tinjil Island to establish a breeding program that will provide monkeys for biomedical research while contributing to Indonesia's primate conservation efforts. A survey was conducted during the summer of 1990 to assess the status of the island's population. Basic demographic data were consistent with those from other field studies of long-tailed macaques, and estimates of reproductivity suggest a successful breeding program. The facility established on Tinjil Island should serve as a useful model for future primate resource and conservation programs. © 1993 Wiley-Liss, Inc.     

Prolonged use of letrozole causes morphological changes on gonads in Galea spixii

Letrozole is used as a therapeutic agent in reproductive disorders caused by high estrogen levels. Letrozole inhibits cytochrome P450 aromatase and reduces estrogen levels. However, the effects of long-term use on reproductive traits are unknown. The aim of this study was to evaluate the prolonged use of letrozole in the gonads of rodents (Spix''s yellow-toothed cavy; Galea spixii). Forty-eight rodents (24 males and 24 females) were randomly divided into the treated and control groups. Letrozole administration started at 15 days of age and continued weekly until 30, 45, 90, and 120 days of age. The body, testis, and ovary weights were analyzed, as well as the morphological progression of spermatogenesis and folliculogenesis. Macroscopically, body weight gain and gonads weight were increased in the letrozole group. Microscopically, the ovaries of treated females showed stratified epithelium and a cellular disorder of the tunica albuginea. In the testes of treated males, the development of seminiferous tubules was delayed and sperm was absent. The collective findings indicate that the prolonged use of letrozole alters secondary sexual characteristics, and causes weight gain, reproductive changes, and male infertility.     

Development of husbandry practices for the captive breeding of key largo woodrats (Neotoma floridana smalli)

The Key Largo woodrat is an endangered rodent endemic to the island of Key Largo in the Florida Keys. After several reports documented a steep decline in the population, the US Fish and Wildlife Service developed a recovery plan, including captive breeding and reintroduction. Captive breeding efforts were to be focused on providing animals for future reintroduction to protected areas on Key Largo. However, little was known about the husbandry needs or reproductive behavior of this elusive nocturnal species. In 2005, Disney's Animal Kingdom^® received 11 animals and began to systematically investigate methods of breeding Key Largo woodrats. Since the program's inception, 30 pups have been born and successfully parent reared. In this report, we describe some of the husbandry techniques that have contributed to the success of the Key Largo woodrat captive breeding program at Disney's Animal Kingdom^®. The results obtained may be of use to other facilities maintaining woodrats and other rodent species. Zoo Biol 30:318–327, 2011. © 2010 Wiley‐Liss, Inc.     

Testing the conspecific attraction hypothesis with Dupont's Larks,a resident species of songbird in central Spain

Some birds use social cues, such as the presence of conspecifics, when selecting breeding habitat. This phenomenon, known as conspecific attraction, has been well‐documented in migratory species, but has not been assessed for resident species of birds. We used Dupont's Larks (Chersophilus duponti) as a model species to determine if conspecific attraction plays a role in habitat selection by resident species of birds. At our study site in Soria province in central Spain, we monitored two potential habitat patches and one managed site where management actions had provided apparently suitable habitat. At each site, we broadcast recordings of the songs and calls of male Dupont's Larks, and monitored their presence during the breeding season and dispersal period in 2018 using automated recorders and field surveys. No birds were attracted to our study sites. Our results suggest that management of patches of suitable habitat should occur close to areas (within 1 km) already occupied by Dupont's Larks to encourage natural colonization because, based on our results, playback of conspecific vocalizations may not attract the species to new breeding areas. However, additional studies are needed before drawing conclusions about the effectiveness of conspecific attraction for this and other resident species of birds.     

Identifying key habitats for the conservation of Bonelli's Eagle Aquila fasciata using radiotracking

Identifying the priority habitats of endangered species is crucial to implementing effective conservation actions. We characterize the key habitats used by Bonelli's Eagle Aquila fasciata, an umbrella and flagship species that is endangered in Mediterranean countries. We radiotracked 17 breeding individuals (10 males and seven females) in Catalonia (NE Iberian Peninsula) and used compositional analysis to determine the key habitats in home‐ranges of both sexes. The main habitats identified within the home‐range area were scrubland, coniferous forests, cropland, sclerophyllous forests, rock outcrops and urban areas, with little difference in habitat use between the sexes. Bonelli's Eagles preferred rocky habitats as breeding areas and scrubland as foraging areas, as these hold the highest abundance of their main prey, Rabbits Oryctolagus cuniculus and Red‐legged Partridges Alectoris rufa. Habitat selection varied seasonally in foraging areas: scrubland was the most preferred habitat type during the breeding season, whereas rocky areas were preferred during the non‐breeding season (although scrubland was also highly selected). Urban areas were avoided both as breeding and as foraging areas. Home‐range size was inversely correlated with the proportion of scrubland, suggesting that this is a key habitat for Bonelli's Eagle. To conserve this species effectively, policies that ensure the preservation of the cliffs used as breeding sites, as well as suitable management of the scrubland used for foraging, should be implemented in the areas inhabited by this species. The promotion of traditional land uses and management techniques that will enhance open areas in Mediterranean landscapes should in the future play an essential role in the conservation of Bonelli's Eagle in Mediterranean habitats.     

Challenges to conservation: land use change and local participation in the Al Reem Biosphere Reserve, West Qatar

One response to humanity's unsustainable use of natural resources and consequent degradation, even destruction of the environment, is to establish conservation areas to protect Nature and preserve biodiversity at least in selected regions. In Qatar, the government has shown strong support for this approach, confronted by the environmental consequences of oil and gas extraction and rapid urban development, by designating about one-tenth of the country a conservation area. Located in the west of the peninsula, it comprises the Al Reem Reserve, subsequently declared a UNESCO Biosphere Reserve. Several approaches have figured in conservation, currently popular is co-management featuring participation of the local population, which recognises that people's activities often contribute to today's environment, with the promotion of bio-cultural diversity. However, these assumptions may not hold where rapid social and cultural change occurs, as in Qatar. We explore the implications of such change, notably in land use. We detail changes resulting with the move from nomadic to sedentary lifestyles: in land access, which now features tribal-state control, and herding strategies, which now feature migrant labour and depend on imported fodder and water, underwritten by the country's large gas and oil revenues. Current stocking arrangements - animals herded in much smaller areas than previously - are thought responsible for the degradation of natural resources. The place of animals, notably camels, in Qatari life, has also changed greatly, possibly further promoting overstocking. Many local people disagree. What are the implications of such changes for the participatory co-management of conservation areas? Do they imply turning the clock back to centrally managed approaches that seek to control access and local activities?     

Genetic assessment of a conservation breeding program of the houbara bustard (Chlamydotis undulata undulata) in Morocco,based on pedigree and molecular analyses

Protection and restoration of species in the wild may require conservation breeding programs under genetic management to minimize deleterious effects of genetic changes that occur in captivity, while preserving populations' genetic diversity and evolutionary resilience. Here, through interannual pedigree analyses, we first assessed the efficiency of a 21-year genetic management, including minimization of mean kinship, inbreeding avoidance, and regular addition of founders, of a conservation breeding program targeting on Houbara bustard (Chlamydotis undulata undulata) in Morocco. Secondly, we compared pedigree analyses, the classical way of assessing and managing genetic diversity in captivity, to molecular analyses based on seven microsatellites. Pedigree-based results indicated an efficient maintenance of the genetic diversity (99% of the initial genetic diversity retained) while molecular-based results indicated an increase in allelic richness and an increase in unbiased expected heterozygosity across time. The pedigree-based average inbreeding coefficient F remained low (between 0.0004 and 0.003 in 2017) while the proportion of highly inbred individuals (F > .1) decreased over time and reached 0.2% in 2017. Furthermore, pedigree-based F and molecular-based individual multilocus heterozygosity were weakly negatively correlated, (Pearson's r = −.061 when considering all genotyped individuals), suggesting that they cannot be considered as alternatives, but rather as complementary sources of information. These findings suggest that a strict genetic monitoring and management, based on both pedigree and molecular tools can help mitigate genetic changes and allow to preserve genetic diversity and evolutionary resilience in conservation breeding programs.     

A species in decline: genetic diversity and conservation of the Victorian eastern barred bandicoot, Perameles gunnii

The eastern barred bandicoot, Perameles gunnii, has undergone a dramatic decline in distribution and abundance on the mainland of Australia during the twentieth century. In 1988 a captive breeding program was initiated to reduce the chance of extinction. With the extinction of the last wild mainland population in the early 1990s, reintroductions from captive-bred P. gunnii have met limited success, and currently only two extant populations persist in predator proof enclosures in the State of Victoria. With ~20 years of breeding, there are concerns that the genetic diversity within the breeding program has declined and may inhibit current and future success of the program. We have used ten nuclear microsatellite loci and sequencing of two partial mitochondrial genes (cytochrome oxidase I and ATPase 6) to determine genetic diversity within current Victorian P. gunnii. These diversity estimates are compared with historic samples from the captive breeding program dating back to 1995, historic samples from the last wild mainland population found at Hamilton in 1992 and contemporary Tasmanian wild populations. Results indicate that the captive P. gunnii population in the State of Victoria has lost significant genetic diversity through time. Genetic diversity is also reduced in populations at Hamilton Community Parklands and Mount Rothwell. Samples from the last wild population at Hamilton collected in 1992, along with samples from Tasmanian P. gunnii, had significantly greater genetic diversity than contemporary mainland populations. The results are discussed with reference to management options for maintaining genetic diversity within Victorian P. gunnii, including crossing Victorian and Tasmanian P. gunnii to increase genetic diversity, adaptability and evolutionary potential.