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1.
We investigated with remote sensing (APEX images) the coexistence of phytoplankton and macrophytes in three interconnected shallow and hypereutrophic fluvial lakes (Mantua Lakes, Northern Italy). High concentrations of chlorophyll-a, up to 60 mg m?3, were determined in the open water between well-developed stands of floating-leaved, submerged, and emergent macrophytes. Our data suggest a general inhibition of phytoplankton by macrophytes, evidenced by decreasing chlorophyll-a concentrations in proximity of macrophyte stands. Chlorophyll-a concentrations halved in the proximity of emergent stands (~6 mg m?3 within 21 m from the stand border) when compared to the outer zones (~13 mg m?3). Contrasting trends were observed for submerged stands, where concentrations decreased inwards from ~8 to ~3 mg m?3. Floating leaved stands had a neutral effect, chlorophyll-a being nearly constant in both inner and outer zones. Overall, remotely-sensed data allow evaluation of quantitative and spatially defined interactions of macrophytes and phytoplankton at the whole ecosystem scale.  相似文献   

2.
Riparian zones are an important strategy to mitigate N and P export to streams. However, their efficiency with respect to nitrate (NO3 ?), ammonium (NH4 +), or soluble reactive phosphorus (SRP) in groundwater remains uncertain in the US Midwest. This study investigates water table fluctuations and NO3 ?, NH4 +, and SRP concentration dynamics in two riparian zone types (outwash vs. glacial till) common in the upper US Midwest. During low water table periods, NO3 ? removal was 93 % at WR (outwash site), and 75 % at LWD (glacial till site); but during high water table periods, NO3 ? removal efficiencies dropped to 50 % at WR, and 14 % at LWD. Median seasonal mass fluxes of NO3 ? removed at WR (9.4–21.7 mg N day?1 m?1 of stream length) and LWD (0.4–1.9 mg N day?1 m?1) were small compared to other riparian zones in glaciated landscapes. The WR site was a small SRP sink (0.114 and 0.118 mg day?1 m?1 during the dry period and wet period, respectively), while LWD acted as a small SRP source to the stream (0.004 mg day?1 m?1 during the dry period; 0.075 mg day?1 m?1 during the wet period). Both LWD and WR acted as sources of NH4 + to the stream with mass fluxes ranging from 0.17 to 7.75 mg N day?1 m?1. Although riparian zones in the US Midwest provide many ecosystem services, results suggest they are unlikely to efficiently mitigate N and P pollution in subsurface flow.  相似文献   

3.
The critical shear stress of resuspension and rates of erosion for cohesive and loosely structured sediments must be obtained by direct measurements since there is no theoretical calculation. An in situ experiment on sediment resuspension was performed in a shallow lake (Langer See, NE Germany; area = 1.27 km2, zmax = 3.8 m) in summer 2006 using a hydrodynamically calibrated erosion chamber (Ø 20 cm). Shear velocity (u*) was incrementally increased in 11 steps (0–2.19 cm s?1) to initiate resuspension events. Entrainment rates (E) of suspended particulate matter (ESPM), total P (ETP), chlorophyll a (EChl a), and soluble reactive P (ESRP) were determined by mass balance. Two subsequent critical u* (0.53 cm s?1 and 1.48 cm s?1) support the ‘two-layered bed’ model of a fluffy surface aggregate layer (freshly deposited phytodetritus prone to resuspension) and an underlying more consolidated biostabilised layer. Patterns in ESPM (2–106 g m?2 h?1), ETP (11–532 mg m?2 h?1), and EChl a (3–24 μg m?2 h?1) revealed a sediment surface maximum of TP and Chl a and their theoretical vertical logarithmic decrease within 4 mm sediment depth, the maximum thickness of sediment layer entrained. The advective ESRP flux (17 mg m?2 h?1) was 43 times higher than the diffusive SRP flux (0.4 mg m?2 h?1). The TP and Chl a micro-profiles suggest that cohesive sediment bed formation is a function of both settling (fluff) and consolidation (biostabilisation). Thus, sediment microstructure and resuspension behavior depend on each other.  相似文献   

4.
Autochthonous production of potamoplankton has recently attracted greater interest as it was incorporated into expanded river concepts such as the flow pulse concept or the riverine productivity model (RPM). This review assembles data on primary production from the River Danube to evaluate the importance of productivity in large rivers. Results indicate positive net production in the middle reach of the river and in impoundments. These sections are characterised by favourable conditions for algal growth. Reduction in flow, reduced concentrations of suspended solids and improved under-water light result in significant increase in plankton biomass. Maximum chlorophyll concentrations were below 20 mg m?3 in 2007 but concentrations up to 130 mg m?3 have been recorded in the past. Since nutrients are not limiting, as in most large rivers, net primary production is largely controlled by availability of photosynthetic active radiation under water, chlorophyll-a, water depth and discharge. Hourly carbon uptake rates of 3–130 mg C m?3 h?1 observed in the Danube are well within the range of 0–790 mg C m?3 h?1 for large rivers of the world. Autochthonous autotrophic production must be regarded as an important feature of large rivers supporting the RPM concept.  相似文献   

5.
Gross primary productivity (GPP) of phytoplankton and planktonic respiration (PR) (i.e., planktonic metabolism) are critical pathways for carbon transformation in many aquatic ecosystems. In inland floodplain wetlands with variable inundation regimes, quantitative measurements of GPP and PR are rare and their relationships with wetland environmental conditions are largely unknown. We measured PR and the GPP of phytoplankton using light and dark biological oxygen demand bottles in open waters of channel and non-channel floodplain habitats of inland floodplain wetlands of southeast Australia that had been inundated by environmental water. Overall, GPP varied from 3.7 to 405.5 mg C m?3 h?1 (mean ± standard error: 89.4 ± 9.2 mg C m?3 h?1, n = 81), PR from 1.5 to 251.6 mg C m?3 h?1 (43.2 ± 5.6 mg C m?3 h?1, n = 81), and GPP/PR from 0.2 to 15.6 (3.0 ± 0.3, n = 81). In terms of wetland environmental conditions, total nitrogen (TN) ranged from 682.0 to 14,700.0 mg m?3 (mean ± standard error: 2,643.0 ± 241.6 mg m?3, n = 81), total phosphorus (TP) from 48.0 to 1,405.0 mg m?3 (316.8 ± 31.4 mg m?3, n = 81), and dissolved organic carbon (DOC) from 1.9 to 46.3 g m?3 (22.0 ± 1.6 g m?3, n = 81). Using ordinary least-squares multiple regression analyses, the rates of GPP and PR, and their ratio (GPP/PR) were modeled as a function of TN, TP, and DOC that had been measured concomitantly. The “best” models predicted GPP and GPP/PR ratio in channel habitats as a function of DOC; and GPP, PR, and GPP/PR in non-channel floodplain habitats as a function of TN and/or TP. The models explained between 46 and 74 % of the variance in channel habitats and between 17 and 87 % of the variance in non-channel floodplain habitats. Net autotrophy (mean GPP/PR 3.0) of planktonic metabolism in our work supports the prevailing view that wetlands are a net sink for carbon dioxide. We propose a nutrient-DOC framework, combined with hydrological and geomorphological delineations, to better predict and understand the planktonic metabolism in inland floodplain wetlands.  相似文献   

6.
Leaf litter plays a critical role in regulating ecological functions in headwater forest streams, whereas the effects of leaves on water quality in urbanized streams are not fully understood. This study examined the potential importance of leaf litter for the release and transformations of organic carbon and nutrients in urban streams, and compared the effects with other types of natural organic substrates (periphyton and stream sediment). Nutrients and organic carbon were leached from senescent leaves of 6 tree species in the laboratory with deionized water, and maximal releases, leaching rate constants, composition and bioavailability of the leached dissolved organic carbon (DOC) were determined. Stream substrates (leaf debris, rocks with periphyton, and sediment) were seasonally collected from urban and forest reference streams of the NSF Baltimore Long-term Ecological Research Site and incubated with overlying stream water to estimate areal fluxes of DOC and nitrogen. Leaf litter leaching showed large ranges in maximal releases of DOC (7.0–131 mg g?1), dissolved organic nitrogen (DON; 0.07–1.39 mg g?1) and total dissolved phosphorus (TDP; 0.14–0.70 mg g?1) among tree species. DOC leaching rate constants, carbon to nitrogen ratios, and DOC bioavailability were all correlated with organic matter quality indicated by fluorescence spectroscopy. Results from substrate incubation experiments showed far higher DOC and DON release and nitrate retention with leaf debris than with sediment, or rocks with periphyton. DOC release from leaf debris was positively correlated with stream nitrate retention at residential and urban sites, with the highest values observed during the fall and lowest during the summer. This study suggests the potential importance of leaf litter quantity and quality on fostering DOC and nutrient release and transformations in urban streams. It also suggests that species-specific impacts of leaves should be considered in riparian buffer and stream restoration strategies.  相似文献   

7.
The kinetic behavior, oxidizing ability and tolerance to m-cresol of a nitrifying sludge exposed to different initial concentrations of m-cresol (0–150 mg C L?1) were evaluated in a sequencing batch reactor fed with 50 mg NH4 +-N L?1 and operated during 4 months. Complete removal of ammonium and m-cresol was achieved independently of the initial concentration of aromatic compound in all the assays. Up to 25 mg m-cresol-C L?1 (C/N ratio of 0.5), the nitrifying yield (Y-NO3 ?) was 0.86 ± 0.05, indicating that the nitrate was the main product of the process; no biomass growth was detected. From 50 to 150 mg m-cresol-C L?1 (1.0 ≤ C/N ≤ 3.0), simultaneous microbial growth and partial ammonium-to-nitrate conversion were obtained, reaching a maximum microbial total protein concentration of 0.763 g L?1 (247 % of its initial value) and the lowest Y-NO3 ? 0.53 ± 0.01 at 150 mg m-cresol-C L?1. m-Cresol induced a significant decrease in the values of both specific rates of ammonium and nitrite oxidation, being the ammonium oxidation pathway the mainly inhibited. The nitrifying sludge was able to completely oxidize up to 150 mg m-cresol-C L?1 by SBR cycle, reaching a maximum specific removal rate of 6.45 g m-cresol g?1 microbial protein-N h?1. The number of SBR cycles allowed a metabolic adaptation of the nitrifying consortium since nitrification inhibition decreased and faster oxidation of m-cresol took place throughout the cycles.  相似文献   

8.
Lipid content and lipid class composition were determined in stream periphyton and the filamentous green algae Cladophora sp. and Spirogyra sp, Sterols and phospholipids were compared to chlorophyll a (chl a) as predictors of biomass for stream periphyton and algae. Chlorophyll a, phospholipids, and sterols were each highly correlated with ash-free dry mass (AFDM) (r2 > 0.98). Stream periphyton exposed naturally to high light (HL) and low light (LL) had chl a concentrations (μg chl a-mg?1AFDM) of 7.9± 0.7 and 12.4 ± 2.9, respectively, while the sterol concentrations of these HL and LL stream periphyton (1.6 ± 0.4) were not significantly different (P > 0.05). Periphyton exposed to an irradiance of 300 μmol photons·m?2s?1 in the laboratory for 60 h had 5.6 ± 0.55 μg chl a·mg?1 AFDM, but the same periphyton exposed to 2% incident light for the same amount of time had 11.0 ± 0.56 μg chl mg?1 AFDM. Sterol concentrations in these periphyton communities remained unchanged (1.5 ± 0.3 μg·mg?1AFDM), Similar results (i.e. changes in chl a but stability of sterol concentrations in response to irradiance changes) were also found for Cladophora and Spirogyra in laboratory experiments. Sterols can be quantified rapidly from a few milligrams of algae and appear to be a useful predictor of eukaryote biomass, whereas cellular levels of chl a vary substantially with light conditions. Phospholipids (or phospholipid fatty acids) are considered to be a reliable measure of viable microbial biomass. Nevertheless, phospholipid content varied substantially and unpredictably among algae and periphyton under different light regimes. Irradiance also had a significant effect on storage lipids: HL Cladophora and HL periphyton had 2 × and 5 × greater concentrations of triacylglycerols, respectively, compared to their LL forms. HL and LL algae also differed in the concentration of several major fatty acids. These light-induced changes in algal lipids and fatty acids have important implications for grazers.  相似文献   

9.
Winter phytoplankton communities in the shallow alkaline pans of Hungary are frequently dominated by picoeukaryotes, sometimes in particularly high abundance. In winter 2012, the ice-covered alkaline Zab-szék pan was found to be extraordinarily rich in picoeukaryotic green algae (42–82 × 106 cells ml?1) despite the simultaneous presence of multiple stressors (low temperature and light intensity with high pH and salinity). The maximum photosynthetic rate of the picoeukaryote community was 1.4 μg C μg chlorophyll a ?1 h?1 at 125 μmol m?2 s?1. The assimilation rates compared with the available light intensity measured on the field show that the community was considerably light-limited. Estimated areal primary production was 180 mg C m?2 d?1. On the basis of the 18S rRNA gene analysis (cloning and DGGE), the community was phylogenetically heterogeneous with several previously undescribed chlorophyte lineages, which indicates the ability of picoeukaryotic communities to maintain high genetic diversity under extreme conditions.  相似文献   

10.
There has been renewed interest in the combined use of high-rate algal ponds (HRAP) for wastewater treatment and biofuel production. Successful wastewater treatment requires year-round efficient nutrient removal while high microalgal biomass yields are required to make biofuel production cost-effective. This paper investigates the year-round performance of microalgae in a 5-ha demonstration HRAP system treating primary settled wastewater in Christchurch, New Zealand. Microalgal performance was measured in terms of biomass production, nutrient removal efficiency, light absorption and photosynthetic potential on seasonal timescales. Retention time-corrected microalgal biomass (chlorophyll a) varied seasonally, being lowest in autumn and winter (287 and 364 mg m?3day?1, respectively) and highest in summer (703 mg m?3day?1), while the conversion efficiency of light to biomass was greatest in winter (0.39 mg Chl- a per μmol) and lowest in early summer (0.08 mg Chl- a per μmol). The percentage of ammonium (NH4–N) removed was highest in spring (79 %) and summer (77 %) and lowest in autumn (47 %) and winter (53 %), while the efficiency of NH4–N removal per unit biomass was highest in autumn and summer and lowest in winter and spring. Chlorophyll-specific light absorption per unit biomass decreased as total chlorophyll increased, partially due to the package effect, particularly in summer. The proportional increase in the maximum electron transport rate from winter to summer was significantly lower than the proportional increase in the mean light intensity of the water column. We concluded that microalgal growth and nutrient assimilation was constrained in spring and summer and carbon limitation may be the likely cause.  相似文献   

11.
The present study is aimed at assessing the extent of arsenic (As) toxicity under three different light intensities—optimum (400 μmole photon m?2 s?1), sub-optimum (225 μmole photon m?2 s?1), and low (75 μmole photon m?2 s?1)—exposed to Helianthus annuus L. var. DRSF-113 seedlings by examining various physiological and biochemical parameters. Irrespective of the light intensities under which H. annuus L. seedlings were grown, there was an As dose (low, i.e., 6 mg kg?1 soil, As1; and high, i.e., 12 mg kg?1 soil, As2)-dependent decrease in all the growth parameters, viz., fresh mass, shoot length, and root length. Optimum light-grown seedlings exhibited better growth performance than the sub-optimum and low light-grown seedlings; however, low light-grown plants had maximum root and shoot lengths. Accumulation of As in the plant tissues depended upon its concentration used, proximity of the plant tissue, and intensity of the light. Greater intensity of light allowed greater assimilation of photosynthates accompanied by more uptake of nutrients along with As from the medium. The levels of chlorophyll a, b, and carotenoids declined with increasing concentrations of As. Seedlings acquired maximum Chl a and b under optimum light which were more compatible to face As1 and As2 doses of As, also evident from the overall status of enzymatic (SOD, POD, CAT, and GST) and non-enzymatic antioxidant (Pro).  相似文献   

12.
Microalgae cultivation systems can be divided broadly into open ponds and closed photobioreactors. This study investigated the growth and biomass productivity of the halophilic green alga Tetraselmis sp. MUR-233, grown outdoors in paddle wheel-driven open raceway ponds and in a tubular closed photobioreactor (Biocoil) at a salinity of 7 % NaCl (w/v) between mid-March and June 2010 (austral autumn/winter). Volumetric productivity in the Biocoil averaged 67 mg ash-free dry weight (AFDW) L?1 day?1 when the culture was grown without CO2 addition. This productivity was 86 % greater, although less stable, than that achieved in the open raceway pond (36 mg L?1 day?1) grown at the same time in the autumn period. The Tetraselmis culture in the open raceway pond could be maintained in semi-continuous culture for the whole experimental period of 3 months without an additional CO2 supply, whereas in the Biocoil, under the same conditions, reliable semi-continuous culture was only achievable for a period of 38 days. However, stable semi-continuous culture was achieved in the Biocoil by the addition of CO2 at a controlled pH of ~7.5. With CO2 addition, the volumetric biomass productivity in the Biocoil was 85 mg AFDW L?1 day?1 which was 5.5 times higher than the productivity achieved in the open raceway pond (15 mg AFDW L?1 day?1) with CO2 addition and 8 times higher compared to the productivity in the open raceway pond without CO2 addition (11 mg AFDW L?1 day?1), when cultures were grown in winter. The illuminated area productivities highlight an alternative story and showed that the open raceway pond had a three times higher productivity (3,000 mg AFDW m?2 day?1) compared to the Biocoil (850 mg AFDW m?2 day?1). Although significant differences were found between treatments and cultivation systems, the overall average lipid content for Tetraselmis sp. MUR-233 was 50 % in exponential phase during semi-continuous cultivation.  相似文献   

13.
Biodegradation of pyridine by a novel bacterial strain, Rhizobium sp. NJUST18, was studied in batch experiments over a wide concentration range (from 100 to 1,000 mg l?1). Pyridine inhibited both growth of Rhizobium sp. NJUST18 and biodegradation of pyridine. The Haldane model could be fitted to the growth kinetics data well with the kinetic constants μ* = 0.1473 h?1, K s = 793.97 mg l?1, K i = 268.60 mg l?1 and S m = 461.80 mg l?1. The true μ max, calculated from μ*, was found to be 0.0332 h?1. Yield coefficient Y X/S depended on S i and reached a maximum of 0.51 g g?1 at S i of 600 mg l?1. V max was calculated by fitting the pyridine consumption data with the Gompertz model. V max increased with initial pyridine concentration up to 14.809 mg l?1 h?1. The q S values, calculated from $V_{ \hbox{max} }$ , were fitted with the Haldane equation, yielding q Smax = 0.1212 g g?1 h?1 and q* = 0.3874 g g?1 h?1 at S m′ = 507.83 mg l?1, K s′ = 558.03 mg l?1, and K i′ = 462.15 mg l?1. Inhibition constants for growth and degradation rate value were in the same range. Compared with other pyridine degraders, μ max and S m obtained for Rhizobium sp. NJUST18 were relatively high. High K i and K i′ values and extremely high K s and K s′ values indicated that NJUST18 was able to grow on pyridine within a wide concentration range, especially at relatively high concentrations.  相似文献   

14.
Global warming is associated with the continued increase in the atmospheric concentrations of greenhouse gases; carbon dioxide, methane (CH4) and nitrous oxide. Wetlands constitute the largest single natural source of atmospheric CH4 in the world contributing between 100 and 231 Tg year?1 to the total budget of 503–610 Tg year?1, approximately 60 % of which is emitted from tropical wetlands. We conducted diffusive CH4 emission measurements using static chambers in river channels, floodplains and lagoons in permanent and seasonal swamps in the Okavango Delta, Botswana. Diffusive CH4 emission rates varied between 0.24 and 293 mg CH4 m?2 h?1, with a mean (±SE) emission of 23.2 ± 2.2 mg CH4 m?2 h?1 or 558 ± 53 mg CH4 m?2 day?1. These emission rates lie within the range reported for other tropical wetlands. The emission rates were significantly higher (P < 0.007) in permanent than in seasonal swamps. River channels exhibited the highest average fluxes at 31.3 ± 5.4 mg CH4 m?2 h?1 than in floodplains (20.4 ± 2.5 mg CH4 m?2 h?1) and lagoons (16.9 ± 2.6 mg CH4 m?2 h?1). Diffusive CH4 emissions in the Delta were probably regulated by temperature since emissions were highest (20–300 mg CH4 m?2 h?1) and lowest (0.2–3.0 mg m?2 h?1) during the warmer-rainy and cooler winter seasons, respectively. Surface water temperatures between December 2010 and January 2012 varied from 15.3 °C in winter to 33 °C in summer. Assuming mean inundation of 9,000 km2, the Delta’s annual diffusive emission was estimated at 1.8 ± 0.2 Tg, accounting for 2.8 ± 0.3 % of the total CH4 emission from global tropical wetlands.  相似文献   

15.
To assess the susceptibility of the base metal budget of a remote tropical montane forest in Ecuador to environmental change, we determined the extent of biological control of base metal fluxes and explored the impact of atmospheric inputs and precipitation, considered as potential drivers of ecosystem change, on the base metal fluxes. We quantified all major base metal fluxes in a ca. 9.1 ha forested catchment from 1998 to 2013. Mean (±s.d.) annual flux to the soil via throughfall + stemflow + litterfall was 13800 ± 1500 mg m?2 Ca, 19000 ± 1510 mg m?2 K, 4690 ± 619 mg m?2 Mg and 846 ± 592 mg m?2 Na of which 22 ± 6, 45 ± 16, 39 ± 10 and 84 ± 33%, respectively, were leached to below the organic layer. The mineral soil retained 79–94% of this Ca, K and Mg, while Na was released. Weathering rates estimated with three different approaches ranged from not detected (ND) to 504 mg m?2 year?1 Ca, ND-1770 mg m?2 year?1 K, 287–597 mg m?2 year?1 Mg and 403–540 mg m?2 year?1 Na. The size of mainly biologically controlled aboveground fluxes of Ca, K and Mg was 1–2 orders of magnitude larger than that of mainly geochemically controlled fluxes (sorption to soil and weathering). The elemental catchment budgets (total deposition ? streamflow) were positive for Ca (574 ± 893 mg m?2) and K (1330 ± 773 mg m?2), negative for Na (?370 ± 1300 mg m?2) and neutral for Mg (1.89 ± 304 mg m?2). Our results demonstrate that biological processes controlled element retention for Ca, K and Mg in the biological part of the ecosystem. This was different for Na, which was mainly released by weathering from the study catchment, while the biological part of the ecosystem was Na-poor. The deposition of base metals was the strongest driver of their budgets suggesting that the base metal cycling of the study ecosystem is susceptible to changing deposition.  相似文献   

16.
Riparian zones are major components of stream ecosystems that influence the physical, chemical, and biological parameters. In particular, the distribution of vertical foliage and the structure of riparian vegetation determine light availability in canopied streams. Here, we analyzed how forest structure will modify light availability and thus affect primary producers’ photosynthetic parameters and the periphyton stoichiometry of mountain streams. We carried out field sampling in four streams with different canopies located in the North-Patagonian Andes and conducted a field experiment in which light conditions were manipulated for four months. Then, we linked our results to qualitative climate change scenarios for North-Patagonian forest predicting how future climate change will affect primary producers and periphyton stoichiometry in low-order streams through modifications in the structure of canopied zones. Finally, we found that biomass, photosynthetic parameters and the elemental content of periphyton exhibited a bell-shaped relationship with light availability which was, in turn, dependent on canopy cover. These trends are characterized by an increase from low light up to 250 μmol m?2 s?1 and a decline when light is over 750 μmol m?2 s?1. Thus, intermediate light resulted in optimal conditions for primary producers’ photosynthesis; however, these intermediate canopied zones are predicted to decrease in the future. Therefore, we predict changes in stream ecosystem stoichiometry due to variations in primary producers’ photosynthesis, and, consequently, periphyton elemental composition as an outcome of forest structure modifications due to climate change.  相似文献   

17.
Methylene blue (MB) biosorption properties of Rhizopus arrhizus were investigated in the presence of surfactants. The effects of cationic and anionic surfactants on MB removal by dead biomass (1 g L?1) were determined. MB removal was tested as a function of initial pH (2–12), contact time (5–1440 min), and dye (37.4–944.7 mg L?1) and surfactant (0–10 mM) concentrations. The opposite charged anionic surfactant dodecylbenzenesulfonic acid sodium salt (DBS) enhanced sorption of cationic MB by biomass dramatically. Maximum biosorption capacity was 471.5 mg g?1 at pH 8 with 0.5 mM DBS at 944.7 mg L?1 MB concentration. The surfactant-stimulated fungal decolorization method may provide a highly efficient, inexpensive, and time-saving procedure in biological wastewater treatment technologies.  相似文献   

18.
A low molecular mass cutinase (designated TtcutA) from Thielavia terrestris was purified and biochemically characterized. The thermophilic fungus T. terrestris CAU709 secreted a highly active cutinase (90.4 U ml?1) in fermentation broth containing wheat bran as the carbon source. The cutinase was purified 19-fold with a recovery yield of 4.8 %. The molecular mass of the purified TtcutA was determined as 25.3 and 22.8 kDa using SDS-PAGE and gel filtration, respectively. TtcutA displayed optimal activity at pH 4.0 and 50 °C. It was highly stable up to 65 °C and in the broad pH range 2.5–10.5. Extreme stability in high concentrations (80 %, v/v) of solvents such as methanol, ethanol, acetone, acetonitrile, isopropanol, and dimethyl sulfoxide was observed for the enzyme. The K m values for this enzyme towards p-nitrophenyl (pNP) acetate, pNP butyrate, and pNP caproate were 7.7, 1.0, and 0.52 mM, respectively. TtcutA was able to efficiently degrade various ester polymers, including cutin, polyethylene terephthalate (PET), polycaprolactone (PCL), and poly(butylene succinate) (PBS) at hydrolytic rates of 3 μmol h?1 mg?1 protein, 1.1 mg h?1 mg?1 protein, 203.6 mg h?1 mg?1 protein, and 56.4 mg h?1 mg?1 protein, respectively. Because of these unique biochemical properties, TtcutA of T. terrestris may be useful in various industrial applications in the future.  相似文献   

19.
The vegetative growth and turion formation of Potamogeton crispus, a submersed aquatic macrophyte, was investigated under a range of phosphorus (P) concentrations (0.025, 0.25, 2.5 and 25 mg P L?1) in the ambient water free of algae, aiming to identify the responses of submersed aquatic macrophytes to nutrient enrichment, a common eutrophication problem in China and worldwide. Plant growth was not affected by different P concentrations in terms of biomass accumulation of stems and leaves. However, the contents of chlorophyll a and starch in plants decreased with increasing water P levels, whereas chlorophyll b and carotenoids declined with P level ranging from 0.025 to 2.5 mg P L?1. The soluble sugar content decreased when water P concentration increased up to 2.5 mg L?1. The P content in plants increased with increasing water P levels, whereas plant N content decreased and soluble protein increased when water P concentration increased over 0.25 mg L?1, implying that P. crispus may have modified its metabolism to adapt to water P availability. When P concentration increased to 25 mg L?1, the number and dry matter production of turions per plant decreased significantly. Meanwhile, there was a significant reduction in turion weight and the accumulations of soluble sugar and starch in turion, when water P concentration was over 0.25 mg L?1. The results suggest that turion formation in P. crispus is sensitive to P concentration in the ambient water, and high P levels may lead to decreases in P. crispus populations due to the decline in turion production.  相似文献   

20.
Energy consumption of municipal wastewater treatment plants can be reduced by the anaerobic pre-treatment of the main wastewater stream. After this pre-treatment, nitrogen can potentially be removed by partial nitritation and anammox (PN/A). Currently, the application of PN/A is limited to nitrogen-rich streams (>500 mg L?1) and temperatures 25–35 °C. But, anaerobically pretreated municipal wastewater is characterized by much lower nitrogen concentrations (20–100 mg L?1) and lower temperatures (10–25 °C). We operated PN/A under similar conditions: total ammonium nitrogen concentration 50 mg L?1 and lab temperature (22 °C). PN/A was operated for 342 days in a 4 L moving bed biofilm reactor (MBBR). At 0.4 mg O2 L?1, nitrogen removal rate 33 g N m?3 day?1 and 80 % total nitrogen removal efficiency was achieved. The capacity of the reactor was limited by low AOB activity. We observed significant anammox activity (40 g N m?3 day?1) even at 12 °C, improving the applicability of PN/A for municipal wastewater treatment.  相似文献   

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