Detection of Arcobacter spp. in the coastal environment of the Mediterranean Sea

The occurrence of Arcobacter spp. was studied in seawater and plankton samples collected from the Straits of Messina, Italy, during an annual period of observation by using cultural and molecular techniques. A PCR assay with three pairs of primers targeting the 16S and 23S rRNA genes was used for detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii in cultures and environmental samples. Only one of the Arcobacter species, A. butzleri, was isolated from seawater and plankton samples. With some samples the A. butzleri PCR assay gave amplified products when cultures were negative. A. cryaerophilus and A. skirrowii were never detected by culture on selective agar plates; they were detected only by PCR performed directly with environmental samples. Collectively, our data suggest that culturable and nonculturable forms of Arcobacter are present in marine environments. The assay was useful for detecting Arcobacter spp. both as free forms and intimately associated with plankton. This is the first report showing both direct isolation of A. butzleri and the presence of nonculturable Arcobacter spp. in the coastal environment of the Mediterranean Sea.     

Detection of Arcobacter spp. in the Coastal Environment of the Mediterranean Sea

The occurrence of Arcobacter spp. was studied in seawater and plankton samples collected from the Straits of Messina, Italy, during an annual period of observation by using cultural and molecular techniques. A PCR assay with three pairs of primers targeting the 16S and 23S rRNA genes was used for detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii in cultures and environmental samples. Only one of the Arcobacter species, A. butzleri, was isolated from seawater and plankton samples. With some samples the A. butzleri PCR assay gave amplified products when cultures were negative. A. cryaerophilus and A. skirrowii were never detected by culture on selective agar plates; they were detected only by PCR performed directly with environmental samples. Collectively, our data suggest that culturable and nonculturable forms of Arcobacter are present in marine environments. The assay was useful for detecting Arcobacter spp. both as free forms and intimately associated with plankton. This is the first report showing both direct isolation of A. butzleri and the presence of nonculturable Arcobacter spp. in the coastal environment of the Mediterranean Sea.     

Plankton community composition and vertical migration during polar night in Kongsfjorden

The polar night in the Arctic is characterized by up to six months of darkness, low temperatures and limited food availability. Biological data on species composition and abundance during this period are scarce due to the logistical challenges posed when sampling these regions. Here, we characterize the plankton community composition during the polar night using water samplers and zooplankton net samples (50, 64, 200, 1500 μm), supplemented by acoustics (ADCPs, 300 kHz), to address a previously unresolved question–which species of zooplankton perform diel vertical migration during the polar night? The protist community (smallest plankton fraction) was mainly represented by ciliates (Strombidiida). In the larger zooplankton fractions (50, 64, 200 μm) the species composition was represented primarily by copepod nauplii and small copepods (e.g., Microcalanus spp., Pseudocalanus spp. and Oithona similis). In the largest zooplankton fraction (>1500 μm), the euphausiid, Thysanoessa inermis, was the most abundant species followed by the chaetognath Parasagitta elegans. Classical DVM was not observed throughout the darkest parts of the polar night (November–mid-January), although, subtle vertical migration patterns were detected in the acoustic data. With the occurrence of a more distinct day–night cycle (i.e., end of January), acoustical DVM signals were observed, paralleled by a classical DVM pattern in February in the largest fractions of zooplankton net samples. We suggest that Thysanoessa spp. are main responsible for the acoustical migration patterns throughout the polar night, although, chaetognaths and copepods may be co-responsible.     

Marine biological invasions in waters of the port of Novorossiysk in the Black Sea

The results of the monitoring of ballast waters of commercial vessels and research on the ecosystem biodiversity of the Novorossiysk Port are reported. Data on structure of immigrant fauna, its abundance, survival and origin of species were obtained. It has been established that the risk of introduction of Mediterranean species into the Novorossiysk Bay is the most probable. The basic “risk groups” are copepods and polychaetes. It has been hypothesized, that ship ballast water can become a primary factor of the “mediterranization” of the copepod fauna (Copepoda) in the northeast of the Black Sea. During the period of research in the Novorossiysk Bay, 36 species of Mediterranean copepods were recorded. A massive number of the cyclopoid copepod Oithona brevicornis Giesbr., 1891, a new species for the Black Sea, was recorded in samples collected in the autumn of 2005 and 2006. The status of the invader was attributed to the polychaete of the Streblospio genus. The necessity of ship ballast water control in Russia and the development of methodology, methods, and regulations for the prevention of the intrusion of pathogenic and potentially dangerous organisms by marine traffic were corroborated.     

Genetic and historic evidence for climate-driven population fragmentation in a top cetacean predator: the harbour porpoises in European water

Recent climate change has triggered profound reorganization in northeast Atlantic ecosystems, with substantial impact on the distribution of marine assemblages from plankton to fishes. However, assessing the repercussions on apex marine predators remains a challenging issue, especially for pelagic species. In this study, we use Bayesian coalescent modelling of microsatellite variation to track the population demographic history of one of the smallest temperate cetaceans, the harbour porpoise (Phocoena phocoena) in European waters. Combining genetic inferences with palaeo-oceanographic and historical records provides strong evidence that populations of harbour porpoises have responded markedly to the recent climate-driven reorganization in the eastern North Atlantic food web. This response includes the isolation of porpoises in Iberian waters from those further north only approximately 300 years ago with a predominant northward migration, contemporaneous with the warming trend underway since the ‘Little Ice Age’ period and with the ongoing retreat of cold-water fishes from the Bay of Biscay. The extinction or exodus of harbour porpoises from the Mediterranean Sea (leaving an isolated relict population in the Black Sea) has lacked a coherent explanation. The present results suggest that the fragmentation of harbour distribution range in the Mediterranean Sea was triggered during the warm ‘Mid-Holocene Optimum’ period (approx. 5000 years ago), by the end of the post-glacial nutrient-rich ‘Sapropel’ conditions that prevailed before that time.     

First record of the calanoid copepod Acartia omorii (Copepoda: Calanoida: Acartiidae) in the southern bight of the North Sea

The occurrence of the calanoid copepod, Acartia omorii, is reportedfor the first time in the coastal waters of the Southern bightof the North Sea, off Calais harbour. Acartia omorii males andfemales were consistently found in four plankton samples. Thecollected specimens were compared with A. omorii individualscollected from the type locality (Tokyo Bay, Japan). The captureof A. omorii, a species native to Japanese coastal waters, isin agreement with the recent observation of the Japanese macroalgaeUndaria pinnatifida within Calais harbor and the hypothesisof passive transport in ship’s ballast water.     

渤海浮游动物群落生态特点Ⅰ.种类组成与群落结构

以１９５９年全国海洋普查浮游动物中网周年标本为材料,对渤海浮游动物的群落结构进行分析。渤海浮游动物群落以近岸广温种为主,主要优势种包括小拟哲水蚤（Ｐａｒａｃａｌａｎｕｓ ｐａｒｖｕｓ）、双毛纺锤水蚤（Ａｃａｒｔｉａ ｂｉｆｉｌｏｓａ）、强额拟哲水蚤（Ｐａｒａｃａｌａｎｕｓ ｃｒａｓｓｉｒｏｓｔｒｉｓ）、拟长腹剑水蚤（Ｏｉｔｈｏｎａ ｓｉｍｉｌｉｓ）、墨氏胸刺水蚤（Ｃｅｎｔｒｏｐａｇｅｓ ｍｃｍｕｒ     

Ontogeny and distribution of Hygophum benoiti(Pisces, Myctophidae) of the North Western Mediterranean

Two species of the lanternfish genus Hygophum, H.benoiti andH.hygomii, are present in the Mediterranean. Differentiationof the larvae from these two species has been problematic. Anattempt to identify such larvae in the plankton samples of theNorth Western Mediterranean led to a broader study that includedthe examination of a large number of plankton samples collectedin the region since 1976. The large number of Hygophum larvaeobtained during July 1992 in the North Western Mediterranean(that proved to be H.benoiti), together with collections oflarvae from different geographic areas (especially H.hygomiifrom the Southwest Indian Ocean), allowed a detailed morphometriccomparison of the larvae of H.benoiti and H.hygomii. Developmentalstages of H.benoiti from yolk sac to post-flexion larvae aredescribed. Information on the temporal occurrence of H.benoitiin the plankton, as well as the spatial distribution of thisspecies during the survey of peak abundance (June/July 1992)in the North Western Mediterranean, is presented.     

Oithona brevicornis Giesbrecht (Copepoda, Cyclopoida) in harborages of the northeastern part of the Black Sea shelf

Cyclopoid species Oithona brevicornis Giesbrecht, a new species for the Black Sea, was found in zooplankton samples collected in autumn of 2005 and 2006 in the Novorossiysk and Tuapse ports of the northeastern shelf of the Black Sea. This species is assumed to have gotten into the Balck Sea with the ballast waters of ships.     

Regional and temporal variation of Oithona spp. biomass, stage structure and productivity in the Irminger Sea, North Atlantic

Oithona spp. standing stock and production is considered relativelystable in space and time as a result of continuous breeding,low metabolism, reduced predation mortality and the abilityof these small cyclopoids to exploit microbial food webs moreefficiently than larger copepods. However, through a reviewof the published literature, we show that Oithona spp. biomasscan vary widely both over the year and with latitude. Thus,the present study set out to investigate the basin scale variabilityin biomass, stage structure and reproduction of Oithona spp.in relation to changes in hydrographic, physico-chemical andbiological parameters encountered during three cruises conductedbetween April and November 2002 in the Irminger Sea, North Atlantic.Here we found that Oithona spp. biomass varied significantlywith temperature and with dinoflagellates biomass concentration.On the other hand, Oithona similis egg production rates increasedwith both ciliates and dinoflagellates concentrations, ratherthan with temperature. The inverse relationship we found betweenOithona spp. naupliar recruitment with Calanus spp. and fishlarvae abundance suggests that predation pressure may contributeto control the spatial variation in the stage structure andbiomass of Oithona spp. and that the nauplii of this genus mayserve as a food source for other planktonic organisms priorto the spring phytoplankton bloom.     

Distribution of potentially pathogenic bacteria as free living and plankton associated in a marine coastal zone

AIMS: To determine the abundance of faecal and nonfaecal bacteria related to human and animal health, as free living or associated with small (>64 microm) and large (>200 microm) plankton, samples were collected monthly from the coastal zone at Messina (Italy). METHODS AND RESULTS: Different enrichment and selective cultural methods were used to determine the abundance of bacteria in sea water and plankton. The bacteria were more frequently isolated from water and large plankton than from small plankton. Vibrio and Aeromonas spp. showed different distribution patterns in water and plankton. Faecal indicators were always present in water and the large size class plankton samples. Enterococci associated with large plankton were more abundant than E. coli in the winter. Vibrio species distributions were different in water and plankton samples. Among arcobacters only A. butzleri was isolated from water and plankton samples. Campylobacter spp. was always absent in small plankton and more frequent in large plankton than in water. CONCLUSIONS: The colonization of zooplankton by potentially pathogenic bacteria is a widespread phenomenon. SIGNIFICANCE AND IMPACT OF THE STUDY: The presence of potentially pathogenic bacteria in sea water and associated with plankton can have ecological and epidemiological implications.     

Basin scale distribution of zooplankton in the Ligurian Sea (north-western Mediterranean) in late autumn

Mesozooplankton biomass and abundance were evaluated in epipelagic waters at 59 stations covering the Italian sector of the Ligurian Sea (north-western Mediterranean) in December 1990. This region is characterised by a cyclonic circulation which encloses a central divergence zone and is associated with a main thermohaline front offshore the western Ligurian coast. At the end of autumn, mesozooplankton biomass (range: 0.80–4.24 mg DW m⁻³) and the abundance (range: 83.8–932 ind. m⁻³) were lower in the divergence zone. On the contrary, in the Ligurian frontal zone at the periphery of the divergence and on the eastern continental shelf the greatest values of biomass and abundance were recorded. Copepods and appendicularians dominated the mesozooplankton community, the main taxa being the copepods Clausocalanus spp. (46% of total zooplankton) and Oithona spp. (15%) and the appendicularian Fritillaria spp. (12%). Three hydrological sub-regions, i.e. the divergence, the eastern continental shelf and the periphery of the divergence, were characterised by different zooplankton communities and characteristic species. Environmental differences between the three zones were mainly related to changes in bottom topography, sea surface temperatures and quantity of particulate organic matter. Vertical mesozooplankton abundance and taxa distribution from the surface to 1,900 m depth were also examined in one station. The results showed that the bulk of the community was concentrated in the upper 200 m, small copepods being dominant particularly in the upper 50 m. The copepod community was more diversified in sub-superficial waters, with a maximum observed in the 200–400 m layer. The distributions of main zooplankton taxa described in epipelagic waters in the eastern Ligurian Sea in autumn were compared with their distribution at surface in the north-western Mediterranean obtained by sampling performed with the Continuous Plankton Recorder in 1997–1999. The analysis of the zooplankton community in CPR samples confirms the dominance of small copepods (Paracalanus spp., Clausocalanus spp., Oithona spp.) and appendicularians in the north-western Mediterranean in late autumn-winter and shows that their distribution is mainly related to the main mesoscale hydrographic features characterising this basin. Guest editors: S. Souissi & G. A. Boxshall Copepoda in the Mediterranean: Papers from the 9th International Conference on Copepoda, Hammamet, Tunisia     

Tracing the introduction history of the brown seaweed Dictyota cyanoloma (Phaeophyceae,Dictyotales) in Europe

Dictyota cyanoloma has recently been described from the Mediterranean Sea and Macaronesia but doubt had arisen as to whether this species was truly native in Europe. The species is mainly found on non-natural substrata (harbour walls, marinas, boat hulls, etc.), strongly suggesting that it is an introduction. Molecular sequence information from historical herbarium samples proves the presence of D. cyanoloma in the Adriatic Sea as early as 1935. Since approximately the year 2000, however, the number of records as well as the geographic range of the species has expanded significantly. The present-day distribution of D. cyanoloma occupies most of the Mediterranean Sea, Macaronesia, NW Africa and southern Portugal, but recent records from Galicia and SW England (Falmouth, Cornwall) indicate that the species is rapidly expanding northward. Collections from Australia demonstrated that the species is also present from Perth in Western Australia, over much of the southern Australian coastline up to Minnie Water in New South Wales. Phylogenetic analyses resolve D. cyanoloma in a sister clade to a previously unreported Australian Dictyota species. Analysis of genetic diversity of the mitochondrial markers (nad6–nad11 and atp9–orf11) reveals that even though Australian populations contain a much higher haplotype richness, European populations are also fairly diverse. Furthermore, only two out of 25 haplotypes are shared between both regions. These somewhat counterintuitive results could be indicative of a more complicated introduction history.     

Molecular characterization of Anisakis larvae from fish caught off Sardinia

Anisakis spp. larvae are parasitic, and potentially zoonotic, nematodes transmitted by marine fish and cephalopods, which are the main intermediate hosts of the third larval stage. The accidental consumption of infected raw or poorly cooked fish may cause gastroenteric diseases and allergies in humans. The aim of the present study was to use polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to define the occurrence, species variability, and host preferences of Anisakis spp. larvae in fish caught off the coast of Sardinia. Necropsy was used on 285 samples; 552 Anisakis spp. L3 larvae were isolated from 87 fish that tested positive for this nematode. Anisakis pegreffii was most frequently encountered (90.6%), with a primary preference for Scomber scombrus, Zeus faber, and Trachurus mediterraneus. In contrast, the prevalence of Anisakis physeteris was only 1.3%. A hybrid genotype of Anisakis simplex sensu stricto and Anisakis pegreffii was also observed, which confirms the results of previous studies carried out in the western Mediterranean. Interestingly, no Anisakis simplex s.s. larvae were recovered. These results indicate that the diversity of Anisakis species is low in Sardinia waters, probably because of its geographic position.     

Pleistocene–Holocene marine ostracods from Mersin offshore sediments,Turkey, Eastern Mediterranean

Ostracod faunas from 18 gravity offshore cores taken from S-SW of Mersin–Ta?ucu harbour in Turkey (Eastern Mediterranean) at water depths comprised between 285 and 665 m were studied. Thirty-two (32) species have been identified. Argilloecia acuminata s.l. and Polycope cf. tholiformis are the dominant species in the studied area. The fauna corresponds very well to the “Argilloecia acuminata community, C11” from the Pleistocene to Holocene established by Sissingh (Sissingh, W., 1982. Ecostratigraphical outline history of the Late Cenozoic ostracode fauna of the Central and Eastern Mediterranean Basin. Proceedings of the Koninklijke Nederlandse Akademie van Wettenschappen B 85, 299–322), and indicates circalittoral to upper bathyal environment in the Mediterranean. The ostracod fauna of the Mersin offshore sediments also shows great similarities to those from the Adriatic Sea (Bonaduce, G., Ciampo, G., Masoli, M., 1975. Distribution of ostracoda in the Adriatic Sea. Pubblicazioni della Stazione Zoologica di Napoli 40 Suppl., 1–304), Sicily (Aiello, G., Barra, D., Bonaduce, G., 2000. Systematics and biostratigraphy of the Plio-Pleistocene Monte S. Nicola section (Gela, Sicily). Bollettino della Società Paleontologica Italiana 39, 83–112) and Bay of Naples (Müller, G.W., 1894. Die Ostracoden des Golfes von Neapel und der angrenzenden Meeresabschnitte. Zoologische Station zu Neapel. Fauna und Flora des Golfes von Neapel, Monographie 31, 1–404). Only a few species are common with the Aegean Sea and Sea of Marmara.     

A blooming jellyfish in the northeast Atlantic and Mediterranean

A long-term time series of plankton records collected by the continuous plankton recorder (CPR) Survey in the northeast Atlantic indicates an increased occurrence of Cnidaria since 2002. In the years 2007 and 2008, outbreaks of the warm-temperate scyphomedusa, Pelagia noctiluca, appeared in CPR samples between 45° N to 58° N and 1° W to 26° W. Knowing the biology of this species and its occurrence in the adjacent Mediterranean Sea, we suggest that P. noctiluca may be exploiting recent hydroclimatic changes in the northeast Atlantic to increase its extent and intensity of outbreaks. In pelagic ecosystems, Cnidaria can affect fish recruitment negatively. Since P. noctiluca is a highly venomous species, outbreaks can also be detrimental to aquaculture and make bathing waters unusable, thus having profound ecological and socio-economic consequences.     

The sub-ice fauna of the Laptev Sea and the adjacent Arctic Ocean in summer 1995

The sub-ice habitat and fauna in the Laptev Sea and the adjacent Arctic Ocean were investigated during the “Polarstern” cruise ARK XI/1 in summer 1995. At the ice-water interface a thin thermo- and halocline developed at many stations due to melting processes. In the lower centi- to decimetres of the ice, an accumulation of organic matter was found (particulate organic carbon: 1.9 mg l⁻¹, chl a: 3.3 μg l⁻¹) that may have provided a food source for the fauna. The water layer directly beneath the ice was inhabited by high numbers of various nauplii (130–23911 ind. m⁻³), and two ecological groups, the pelagic sub-ice fauna that originates from the surface water plankton, and the sympagic sub-ice fauna that migrates into this boundary layer from the ice interior. The pelagic fauna dominated the sub-ice community both in terms of species number and abundance. Both groups mainly comprised small copepods (e.g. Oithona similis, Oncaea borealis, Pseudocalanus spp., Halectinosoma spp., Tisbe spp.), but foraminifers and pteropods, for example, also occurred regularly. Diversity was generally low. Factors influencing the composition and abundance of the sub-ice fauna were most likely water depth, salinity and sea-ice sediments. Accepted: 6 July 1998     

Genetic identification of Anisakis larvae in European hake from Atlantic and Mediterranean waters for stock recognition

The occurrence of seven species of the larval parasitic nematode Anisakis , which can be used as a biological tag for hake Merluccius merluccius stocks throughout their geographical range, is reported. Hake were collected from 14 localities in the Mediterranean Sea and the Atlantic Ocean. Anisakis larvae ( n  = 1950), which were recovered, were identified to species by means of genetic markers (allozymes). Within Anisakis type I, the larvae of A. pegreffii , A. simplex s.s ., A. typica and A. ziphidarum were detected, while within Anisakis type II, A. physeteris , A. brevispiculata and Anisakis sp. were identified. There were significant differences in the relative proportions of the various Anisakis species identified in hake samples from the Mediterranean Sea and Atlantic Ocean, suggesting the existence of different stocks of M. merluccius in European waters.