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1.
Samples of three seed lots of each of three cultivars of carrot, celery and onion were primed in polyethylene glycol solution for 2 weeks at 15 °C. Priming reduced the mean germination times (recorded at 15 °C) of all seed lots (compared to the untreated control) by 3–4 days in carrot, 6–10 days in celery and 3–5 days in onion. The largest reductions in mean germination time occurred in the slowest-germinating seed lots. There were highly significant interactions between priming and cultivars, and between priming and seed lots within cultivars for each species. Drying back the primed seeds at 15 °C increased the mean germination times (compared to primed seed which had not been dried) by 0·6 day in carrot and 1·4 days in celery, and there was no interaction with cultivars or seed lots. The corresponding increase for onion was either 1·0 or 1·8 days, according to the cultivar, but this variation was largely attributable to differences in time taken for the dried seeds to re-imbibe. Seeds dried back at 30 °C germinated 0·2·0·7 day (depending on the species) later than those dried at 15 °C. Percentage germination was not affected by either priming or drying back. Priming reduced the spread of germination times in all cultivars. For primed and dried-back seed, the spread of germination times was larger than that of primed seed in certain cultivars, but was always smaller than that of untreated seeds. 相似文献
2.
Effects of osmotic priming and ageing on the germination and emergence of carrot and leek seed 总被引:3,自引:0,他引:3
Carrot and leek seed was osmotically primed in polyethylene glycol solution (273 g/kg water and 342 g/kg water respectively) for 10, 14 or 17 days before accelerated ageing for 0, 24, 48, 72 or 96 h. Priming reduced the germination time compared with non-primed seed. Accelerated ageing increased germination and emergence times and decreased percentage germination and emergence to a greater extent for the primed seeds than for non-primed seeds in both species. Primed and dried but non-aged seed from both species was stored at 10°C for 12 months. There was no loss of viability and improvements in germination time due to priming were maintained throughout the storage period for all the priming treatments in leek, and for the 10 and 14 day priming treatments in carrot. Carrot seed primed for 17 days lost some viability after 12 months storage compared with non-stored seed. 相似文献
3.
Beneficial microorganisms (Clonostachys rosea IK726, Pseudomonas chlororaphis MA342, Pseudomonas fluorescens CHA0, Trichoderma harzianum T22 and Trichoderma viride S17a) were successfully applied to carrot and onion seed during a commercial drum priming process. Applied microorganisms were recovered above the target of at least 1 × 105 cfu g−1 seed following subsequent application of pesticides to the seed according to standard commercial practices of film-coating carrot and pelletting onion seed. Two glasshouse experiments consistently showed that priming improved emergence of carrot seed and that C. rosea IK726 further improved emergence time. Priming improved emergence of onion seed in one glasshouse experiment, but had an unexpected negative effect on emergence in the second experiment, possibly due to the proliferation of an unidentified indigenous microorganism during priming, becoming deleterious in high numbers. In this experiment, the application of beneficial microorganisms during priming negated this effect and significantly improved emergence. For each crop, a series of field trials was also carried out over three years, at two different sites each year. Although some positive effects of different seed treatments were seen on emergence or yield in individual field trials, no consistent effects were found for primed or microorganism-treated seed across all sites and years. However, a combined analysis of data for all years and sites indicated that pesticide application did consistently improve emergence and yield for both carrot and onion. This is the first comprehensive study assessing glasshouse and field performance of carrot and onion seed primed with beneficial microorganisms during a commercial process of drum priming in the UK. 相似文献
4.
The effects of re-use of polyethylene glycol priming osmotica upon the development of microbial populations and germination of leeks and carrots 总被引:1,自引:0,他引:1
Three batches of leek seeds were osmotically primed successively in the same polyethylene glycol solution in a bubble column at a seed concentration of 100 g/litre for seven days at 15°C. Three batches of carrot seeds were similarly primed in a separate solution for six days at 15°C. The concentration of microorganisms in the solutions increased rapidly during priming of the first seed batch for both seed types, but increases during priming of the second and third batches were small. The seeds were the main source of the microorganisms; priming reduced the numbers of colonies of filamentous fungi and increased those of bacteria and yeasts. The priming treatments improved the percentage germination of the three seed batches of primed carrots and reduced the mean time to germination in both species and the mean time to emergence in compost. Percentage emergence was not affected by priming except in the third batch of primed carrot seed. The presence of large numbers of microorganisms in the priming solutions did not greatly affect seed performance when the same osmoticum was used three times with leeks and twice with carrots. Priming did not affect the number of abnormal seedlings. 相似文献
5.
Leek seed lots of high (91%) and low (82%) viability were primed in aerated polyethylene glycol (PEG) solutions in Bubble-columns and by a non-osmotic priming technique in both 1987 and 1988 and the seeds were then sown in the field. Both methods of priming established a similar seed moisture content during treatment and, in the laboratory, produced seeds with more rapid and uniform germination than untreated seeds, with a greater advantage for Drum compared with Bubble-column priming in PEG. In the field, both priming techniques gave seedling emergence responses similar to those from priming in PEG by the laboratory-scale technique on filter paper. Both large-scale priming methods gave earlier and more uniform emergence than untreated seeds and gave similar or slightly higher levels of seedling emergence, except on one sowing occasion when seeds were stored before sowing followed by sowing into a drying seedbed. 相似文献
6.
Samples of carrot, celery, leek and onion seed were treated before sowing by imbibition in osmotic solutions of polyethylene glycol 6000 (PEG), glycerol and potassium dihydrogen orthophosphate (KH2PO4). The solutions were sufficiently concentrated to prevent germination during treatment, and within each species, the amount of water taken up by the seeds during treatment did not vary greatly between solutions. All treatments increased the rates of seed germination and seedling emergence compared to untreated controls, but glycerol-treated seeds germinated and emerged significantly more slowly than did PEG- and KH2PO4-treated seeds. The effects of PEG and glycerol treatments on percentage germination and seedling emergence were small, but KH2PO4 treatment tended to reduce percentage germination and emergence, most markedly in leek and one cultivar of celery. It is concluded that PEG treatment gives the most consistently beneficial effects for the range of species tested. 相似文献
7.
The upper-temperature limit for germination of cv. Celebrity celery seeds incubated in darkness in laboratory tests was slightly raised following a seed soak with a mixture of the gibberellins A4 and A7 + ethephon (G + E) but more so by osmotic priming with polyethylene glycol (PEG). However, the best effects were obtained with a combination of growth regulator and priming treatments. Incorporation of growth regulators into the PEG was more effective than soaking seeds in G + E solutions either before or after osmotic priming. In glasshouse tests, seedling emergence was more rapid, percentage emergence was higher and seedling weight was greater from seeds treated with PEG either alone or in combination with G + E than from untreated seeds, water-soaked seeds or seeds given G + E soaks before or after PEG treatment. 相似文献
8.
Osmotic priming of carrot seeds for 2 wk in polyethylene glycol (PEG, — 10 MPa) at 15 °C led to more rapid and synchronous germination at 20 °C compared to untreated seeds. These responses were enhanced by a 24 h pre-priming soak in water or a change of solution after the first 24 h of priming to remove leachate. The inclusion of 200 mg litre-1N-substituted phthalimide in the pre-priming soak and/or in the PEG further enhanced the results of priming. Leachate removal combined with phthalimide inclusion gave 79% and 86% germination from seeds of two carrot cultivars during the first day in 20°C water following priming. In contrast, cumulative germination of untreated seeds of the same cultivars was 18% and 61% respectively after 3 days in 20°C water. Seeds primed in PEG containing 200 mg phthalimide litre-1with the solution replaced after the first 24 h germinated earlier and more synchronously than untreated seeds over a range of germination temperatures (5, 10, 15, or 20°C), but the effects of priming were most marked at 5°C. 相似文献
9.
Atique-ur-Rehman Muhammad Farooq Zahid Ata Cheema Abdul Wahid 《Plant Growth Regulation》2012,68(2):189-201
Boron (B) is amongst the important micronutrients required for rice from start till physiological maturity. This study was conducted to explore the role of boron application in seedling emergence, leaf appearance and elongation, chlorophyll content, water relations and yield related traits of fine rice. Boron was applied as seed priming. For priming seeds of fine rice cultivars Super Basmati and Shaheen Basmati were soaked in 0.001 and 0.01?% aerated B solutions (w/v); while untreated dry seeds and water soaked (hydropriming) seeds were taken as control. Substantial improvement in seedling emergence was noted by seed priming in 0.001 and 0.01?% solutions. Rate of leaf emergence and elongation and tiller appearance were also improved in seedlings raised from seeds primed in 0.001?% B solution in the tested cultivars. Likewise, leaf chlorophyll contents were significantly improved by B especially with 0.001?% concentration; as was the case for water relations of rice cultivars. At final harvest, all yield contributing parameters were improved by B priming. Increase in yield was due to decrease in panicle sterility by B treatments. A linear increase in leaf and grain B contents was observed with increase in concentration of B priming solution. Overall, B application at very low rate substantially improved seedling emergence, leaf appearance and elongation, tillering, chlorophyll, water relations and yield related traits resulting in better yield and grain B contents. In this regard, seed priming offers an effective and pragmatic way of B application. 相似文献
10.
D. GRAY 《The Annals of applied biology》1984,104(3):559-565
The germination and emergence characteristics, and early seedling growth, of carrot seeds cv. Chantenay red-cored from different sources with a range of germination from 54–94%, was compared. Seeds from protected crops (mean temperature of growth 21°C, r.h. 45–70%) gave higher percentage germination than those from crops grown outdoors (mean temperature 15°C, 70–100% r.h.). Germination was also higher from mature (seed moisture content at harvest 20% or lower) than immature seed (seed moisture content at harvest between 20 and 60%). High percentage germination (>90%) was associated with low mean germination times and low spreads of germination times whilst the reverse was true for low percentage germination. Similar relationships were found for seedling emergence characters in the field although a lower proportion of the viable seeds produced seedlings from slowly than rapidly germinating seed lots. In general, seed lots having a low percentage germination gave greater variability in plant weight than those of higher percentage germination. There was no effect of seed source on radicle or shoot relative growth rates or on post-emergence seedling growth rates. 相似文献
11.
Responses of Vegetable Seeds to Controlled Hydration 总被引:1,自引:1,他引:0
Leek, onion and carrot seeds were imbibed in water and in solutionsof polyethylene glycol (PEG) 6000 over the range 0.5to 4.0 MPa osmotic potential, for periods up to 28 dat 15 C. Seeds started to germinate after 7 and 14 d at 0.5MPa and 1.0 MPa PEG, respectively, but in the lattercase, germination did not exceed 5%. No germination occurredin solutions of lower (more negative) osmotic potential. Seedmoisture content increased with osmotic potential in all threespecies and the relationships were unaffected by the durationof imbibition in solutions of 1.0 to 4.0 MPa,though leek seeds had higher moisture contents than the otherspecies for any given osmotic potential. Linear relationships between response to priming (differencebetween mean germination times of primed and untreated seeds)and seed moisture content were obtained for each species, positiveresponses being obtained above 3035% seed moisture contentwith optima at 46, 44.5 and 44% seed moisture contents in leek,onion and carrot, respectively. Priming had no effect on embryovolume or cell number per embryo in leek and onion. Carrot embryovolume increased by 43% and cell number per embryo doubled inprimed compared with untreated seeds, whereas seeds imbibedin water showed only a slight increase in cell number per embryoat the stage when radicles were beginning to penetrate the seedcoat. Allium cepa L. cv. Rijnsburger Robusta, onion, Allium porrum L. cv. Winterreuzen, leek, Daucus carota L. cv. Nantaise, carrot, germination, priming, polyethylene glycol, seed moisture, cell number, embryo volume 相似文献
12.
Effects of matriconditioning on onion seed germination, seedling emergence and associated physical and metabolic events 总被引:1,自引:0,他引:1
The effect of matriconditioning, the physiological presowing seed technique, using Micro-Cel E on Allium cepa L. cv. Czerniakowska seed quality was studied. Several ratios of seeds, carrier, water and time of priming were tested. The most effective treatment for improving onion seed germination at most tested temperatures was priming to a ratio of 2 g seed:1 g Micro-Cel:3 g water for 5 days in light at 15 °C. Matriconditioning greatly improved the germination and emergence percentage, seedling fresh and dry weight and reduced electrolyte leakage compared to that of untreated seeds; this beneficial effect was especially evident at suboptimal temperatures. Matriconditioning improved the germinability of aged seeds, the effect being more pronounced in the more aged seeds. No significant differences in ethylene production by primed and non-primed seeds were observed in the absence of its precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), but its presence during imbibition caused an increase in ethylene production; an enhanced activity of in vivo ACC oxidase in Allium cepa matriconditioned seeds in comparison to untreated seeds, indicates that the endogenous level of ACC is a limiting factor of ethylene production. Likewise, the activity of ACC oxidase isolated from matriconditioned seeds was higher than that from untreated seeds. Higher endo--mannanase and total dehydrogenase activities were observed in primed air-dried seeds in comparing to non-primed seeds. 相似文献
13.
《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》1998,321(8):705-711
Seed priming (controlled imbibition) is a widely used technique for improving crop establishment, because it allows a reduction of the time to radicle emergence following seed imbibition and synchronization of individual seeds within seed lots with respect to germination timing. The major problem encountered in seed priming is the control of seed imbibition to a level permitting pre-germinative processes to proceed but that blocks radicle emergence. If not, the consequence of drying back the seeds to initial moisture content for storage purposes could be a total loss of the treated batch. This is because, as long as radicle growth has not begun, seeds may be re-dried without any permanent deleterious effects upon subsequent germination or growth. Recently, we reported the discovery of a molecular marker of sugar beet seed priming, corresponding to the basic B-subunit of the seed storage protein 11S globulin. An ELISA based upon this molecular marker has been used to analyse how different sugar beet seed lots respond to a priming treatment. The results demonstrate that this ELISA allows us to readily distinguish between the primed seeds and the corresponding untreated seeds. 相似文献
14.
Muhammad Farooq Tariq Aziz Hafeez ur Rehman Atique ur Rehman Sardar Alam Cheema Tariq Aziz 《Acta Physiologiae Plantarum》2011,33(5):1707-1713
This study was conducted to investigate the benefits associated with re-drying after seed priming with polyamines. Wheat (cv.
AS-2002) seeds were soaked in 10 and 20 mg L−1 aerated solutions of spermidine (Spd), putrescine (Put) and spermine (Spm), and distilled water (CK2) for 12 h at 28 ± 2°C.
Untreated seeds (CK1) and priming in distilled water (CK2) were taken as control treatments. Seeds were primed in two sets:
In one set, after each treatment, seeds were given three surface washings with distilled water and dried closer to original
moisture; in the other, seeds were only surface dried and used immediately. Use of surface-dried seeds after priming was more
effective since it reduced emergence time and synchronized the emergence. Moreover, final emergence, shoot and root length,
seedling fresh and dry weight were also improved. Improved starch metabolism was considered possible reason of seed invigoration.
All the seed treatments resulted in a lower electrical conductivity of seed leachates compared with control; however, there
was more decrease in seeds re-dried after priming than the seeds surface dried after priming. Although the effect of all the
polyamines was stimulatory, Spd was the more effective for most of the attributes studied. Nonetheless, Put was more effective
for seedling fresh and dry weights. All the polyamines were more effective at lower concentrations except Spm, which improved
the coefficient of uniformity of emergence at high concentration. To conclude, if immediate sowing is possible, use of surface-dried
seeds after priming may be more effective; seed priming with 10 mg L−1 Spd was the most effective technique when surface dried. 相似文献
15.
《农业工程》2021,41(5):491-498
In this study, the effect of seed priming using ascorbic acid (ASA) on three commercial wheat cultivars i.e., Punjab-2011, Faisalabad-2008, and Ujala-2016 under salinity stress in both homogenous and heterogeneous environments has been investigated. It revealed that different levels of salinity have significantly reduced the growth attributes like percent germination, germination index, radical & plumule length, seed vigor index (In-vitro), seedling length, fresh & dry weight, and total chlorophyll content (In-vivo) with subsequent treatments. Salinity stress was induced by using NaCl in three different concentrations (100, 150, and 200 mM). Seeds of the three cultivars primed with 50, 100, and 150 mg/L ascorbic-acids have not only improved percent germination but also considerably reduced germination time and increased germination index (GI) indicating the potential for tolerating saline conditions. Seedling growth (seedling length, Fresh weight, and dry weight) of seeds primed with 50, 100, and 150 mg/L (ASA) was significantly higher than other non-primed seeds under the prevailing saline conditions. Hormonal priming with different concentrations of ascorbic acid was effective, nevertheless, the best results were obtained with 100 and 150 mg/L (ASA) concentrations. We concluded that the delay in germination and seedling growth was mainly due to excessive Na+ accumulation in the seeds of wheat cultivars. On the other hand, seed priming with various concentrations of ascorbic acid has proved to be effective in inducing salt tolerance in terms of germination parameters, seedling characteristics, and chlorophyll retention in the three local commercial wheat cultivars. 相似文献
16.
Saddam Hussain Abdul Khaliq Mohsin Tanveer Amar Matloob Hafiz Athar Hussain 《Acta Physiologiae Plantarum》2018,40(4):68
Though seed priming has been emerged as an effective and pragmatic approach, efforts are being made to discover and optimize the new priming agents which are cheaper and easily accessible to the farmers. Here, we established two independent experiments, to ascertain the role of aspirin priming in salinity tolerance of wheat. In the first experiment, various concentrations of aspirin (125, 250, 375, and 500 ppm) were examined for emergence and seedling growth of wheat. A non-primed control, hydropriming, and hydrogen peroxide priming treatments were also maintained for comparison. Among the different treatments, seeds primed with 125 and 250 ppm aspirin depicted better emergence, vigorous seedling growth, and higher starch metabolism. Therefore, these treatments were further used in the second experiment under salinity stress (10 dS m?1). Salinity stress caused delayed and erratic emergence hampered the shoot and root growth, chlorophyll contents, and enhanced the lipid peroxidation and phenolics content in wheat seedlings. However, wheat seed priming particularly with aspirin effectively alleviated the negative effects of salinity on most of the observed parameters. Aspirin priming also significantly enhanced the activities of antioxidant enzymes (catalase, peroxidase, and superoxide dismutase), and reduced oxidative stress in wheat seedlings. Vigorous growth and greater salinity tolerance of wheat seedlings derived from aspirin primed seeds were related with better starch metabolism, strong antioxidative defense system, and lower lipid peroxidation. 相似文献
17.
The yield and performance of seeds from crops of winter-hardy, bolting-resistant onion grown at temperatures of 15–16, 18–19 and 22–23°C in 1979, 1980 and 1982 were compared. Yields of seed from crops grown at 22–23°C were lower than those from crops grown at lower temperatures but the seeds ripened between 11 and 32 days earlier. Seeds from crops grown at mean temperatures of above 18°C gave higher percentage germination when imbibed at 30°C than 20°C and they also gave higher percentage seedling emergence than those from crops grown at lower temperatures. Seedlings from seeds produced at mean temperatures above 18°C were heavier than those from seeds of a similar weight but produced at lower temperatures. None of these differences were associated with differences in seed weight, embryo weight or seed dormancy but were positively correlated with differences in seed N-concentration. The differences were also associated with the rate of imbibition of water as high germination, high N-content seeds had a slower rate of imbibition than low germination, low N-content seeds of the same weight. 相似文献
18.
Effects of osmotic priming and ageing on onion seed germination 总被引:3,自引:0,他引:3
Onion seeds were osmotically primed in polyethylene glycol solution (342 g/kg water) either for 14 days before accelerated ageing at 40°C. 18% m.c. for 0, 24, 48, 72 or 96 h, or for 10, 14 or 17 days after ageing. Priming improved the rate of germination compared with non-primed seed. Priming before ageing delayed the loss of viability due to ageing, but priming after ageing had no effect on viability. Primed and dried onion seed was stored for 18 months at 10°C, 9% m.c. with no effect on viability; improvements in germination rate due to priming were maintained over the storage period. Conductivity measurements of seed leachates were not a consistently reliable indicator of germination performance. 相似文献
19.
Elena V. Popova Du Hyun Kim Sim Hee Han Elena Moltchanova Hugh W. Pritchard Yong Pyo Hong 《Acta Physiologiae Plantarum》2013,35(10):3015-3025
Biodiversity conservation programmes are underpinned by seed banking following drying to low water contents (WC), and supported by both the assessment and prediction of seed viability over time. The means of judging viability is thus crucial to the comprehension of seed vigour. We selected seeds of three species and one hybrid in the Salicaceae likely to have variation in tolerance to drying, processing and storage, including in relation to cryobanking, and compared survival growth as radicle emergence (germination) and normal seedling production. With three seed lots of Salix gracilistyla, air-drying to 8–10 % WC enhanced seed survival after 40 days’ storage at 5 °C as compared with non-treated seeds at 14–20 % WC. Four seed lots of Populus alba × P. glandulosa showed equally high germination (88–100 %) and proportions of normal seedlings (81–99 %) when stored at 5 °C for 7–10 weeks. Among seven seed lots of S. gracilistyla, two groups with different storage behaviour could be statistically distinguished with normal seedling production ranging from 0 to 45 % after storage at 5 °C for 13 weeks. Seed tolerance to WC manipulation and cryopreservation was very variable among species and seed lots. Seed lots of S. hallaisanensis and S. gracilistyla with ~80 % germination survived cryopreservation at 10 % WC, but were sensitive to lower WCs. In contrast, Populus seeds had greater desiccation tolerance combined with cryopreservation capability. With seed lots of all species and hybrids, cryopreservation had little effect on viability unless the high moisture freezing limit had been exceeded (~10–20 % WC, depending on seed lot). However, under all conditions of handling (drying, rehydration, storage at 5 °C or cryopreservation) using germination as the only indicator of viability over-estimated survival compared with normal seedling production. 相似文献
20.
Parsnip seeds from four years of seed production (1980–83) were used to examine the effects of position of the seed on the parent plant and several cultural factors on seed and embryo growth and seedling performance. Maximum seed dry weight was reached c. 50 days after flowering (DAF) but embryos continued to grow for a further 7–15 days. The coefficient of variation (C.V.) of seed weight fell from c. 35–40% at 40 DAF, to about 20–25% at maturity. The corresponding figures for C.V. of embryo length were 25–35% at the early harvest and 12–15% at maturity. The effects of seed position, plant density, harvest method, seed grading after harvest on seedling emergence and weight characters were small. Harvesting seeds c. 60 DAF gave, on average, 3.3 days earlier emergence, c. 3.6 days lower ‘spread’ of emergence time and c. 9% higher seedling emergence than harvesting 2 wk earlier. Late harvesting of the seed crop reduced the C.V. of seedling weight from 53 to 46%, on average, but there were variations in response from year to year. Seedling emergence and seedling weight characters were more closely related to embryo length than to seed weight characters. 相似文献