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1.
R. E. B. Ketchum D. M. Gibson L. Greenspan Gallo 《Plant Cell, Tissue and Organ Culture》1995,42(2):185-193
Three cell lines of Taxus brevifolia Nutt. with differing growth rates were used to assess the effects of basal salt mixtures, carbohydrates, organic nitrogen additives, vitamin formulations, and plant growth regulators on callus growth. Gamborg's B5 major salts provided significantly better growth than all other salt formulations tested. The greatest biomass was obtained with 1% total carbohydrate. The best carbohydrate combination, 0.5% fructose + 0.5% sucrose, was significantly better than all other combinations of carbohydrates tested. A complex vitamin mixture was significantly better than any one previously published vitamin formulation. Greatest rates of callus growth were obtained with 4.14 M (1 mg l-1 picloram, 0.46 M (0.1 mg l-1 kinetin, and 0.38 M (0.1 mg l-1) abscisic acid or 0.29 M (0.1 mg l-1 gibberellic acid. Our final medium, TM5, is superior to published methods for the general callus culture of T. brevifolia. This medium has improved growth in three tested cell lines to provide doubling times of 3.5 to 5.6 days, an average 5.3-fold increase over our previously published medium.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- 2,4,5-T
2,4,5-trichlorophenoxyacetic acid, 2ip-6-(,-dimethylamino)-purine
- ABA
abscisic acid
- BA
6-benzyladenine
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- kinetin
6-furfurylaminopurine
- NAA
napthaleneacetic acid
- picloram
4-amino-3,5,6-trichloropicolinic acid 相似文献
2.
Chang-Heon Kim Seong-Hyun Hong Sung-Ho Son In-Sik Chung 《Biotechnology and Bioprocess Engineering》1999,4(4):273-276
Two-phase cultures ofTaxus cuspidata were performed using silicone cubes as a second phase in shake flasks for paclitaxel production. Among various taxanes, paclitaxel
was selectively adsorbed on the silicon cubes. When silicone cubes were added to suspension culture ofTaxus cuspidata, paclitaxel production increased about 45 folds. The maximum paclitaxel production was 3.95 mg/L when 10% of silicone cubes
were added to the culture at the 7th day from inoculation. 相似文献
3.
Effects of a fungal endophyte, Fusarium mairei, on growth and paclitaxel formation of Taxus cuspidata cells were investigated by adding fungal endophyte culture supernatant (FECS) to suspension cultures of T. cuspidata cells. The main effective chemical responsible for paclitaxel formation in FECS was an exopolysaccharide (EPS) of molecular
weight ~2 kDa. FECS fractions except EPS stimulated growth of Taxus cells but had no effects on paclitaxel accumulation. Additionally, elicitation efficiency of FECS based on different culture
conditions was studied. EPS content in FECS was related to FECS culture conditions. FECS with long cultivation and high-aeration
cultivation contained higher EPS content and resulted in higher paclitaxel yield than that with short cultivation and low-aeration
cultivation. The maximum yield of paclitaxel from Taxus cultures, elicited by FECS with 9-day cultivation, was 4.7-fold that of the control cultures. 相似文献
4.
Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis 总被引:18,自引:0,他引:18
Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium. Addition of 50 mg chitosan l–1, 60 M methyl jasmonate and 30 M Ag+ resulted in the greatest paclitaxel production, at 25 mg l–1 in the cultures, being almost 40 times higher than that of the control culture, 10 times higher than that of the culture exposed to Ag+, 6 times higher than that of the culture elicited by chitosan and almost double that of the culture elicited by methyl jasmonate. 相似文献
5.
Taxus callus cultures: Initiation,growth optimization,characterization and taxol production 总被引:10,自引:0,他引:10
Enaksha R. M. Wickremesinhe Richard N. Arteea 《Plant Cell, Tissue and Organ Culture》1993,35(2):181-193
Callus was induced from Taxus baccata cv. Repandens Parsons ex Rehd., T. brevifolia Nutt., T. cuspidata Sieb. & Zucc., and T. x media cvs. Hicksii and Densiformis Rehd. using different concentrations of 2,4-d-(2,4-dichlorophenoxyacetic acid), IBA (indole-3-butyric acid), or NAA -naphthalene acetic acid in combination with kinetin. All cultures grew slowly following the first subculture, and a majority turned brown and ceased growth within the next six to twelve months. The callus cultures which lived, continued to grow very slowly for one to two years before the growth rate improved. Initiation of roots and shoot primordia-like structures occurred on some cultures maintained in the dark, and 16 h light/8 h dark, respectively. A fast-growing, habituated callus line (CR-1) derived from T. x media Rehd. cv. Hicksii was established from callus initiated in 1986. Supplementing the medium with casein hydrolysate and both fructose and glucose enhanced the growth rate. A great deal of heterogeneity was found among and within the callus, with respect to the amount of taxol produced. The callus exhibited levels of taxol ranging from 0.1 to 13.1 mg kg-1 (0.0001 to 0.0131%) on a dry weight basis. Overall, the older brown-colored callus produced more taxol than the younger pale yellow-colored callus. The presence of taxol in callus samples was established by high performance liquid chromatography, its biological activity confirmed by a microtubule-stabilizing bioassay and its structure confirmed using one-and two-dimensional 1H and 13C nuclear magnetic resonance spectroscopy.Abbreviations 2,4-d
2,4-dichlorophenoxyacetic acid
- IBA
indole-3-butyric acid
- NAA
-naphthaleneacetic acid
- kinetin
6-furfurylaminopurine
- 2iP
6-(,-dimethylallylamino)purine 相似文献
6.
An efficient in vitro method for mass propagation of a woody ornamentalIxora coccinea L. 总被引:1,自引:0,他引:1
Various factors that affect culture establishment, shoot growth, proliferation and rooting ofIxora coccinea L., a woody shrub, were studied. Stem cuttings (decapitated shoot, three nodes) were the most suitable explants for multiple-shoot proliferation, and when cultured on a woody plant medium (WPM) containing 2.5 M BA produced axillary shoots which branched repeatedly, yielding an average of 27 shoots per explant after 6 weeks in culture. Kinetin, 2-iP, zeatin and thidiazuron all induced multiple-shoot formation, but were less effective than BA. While the presence of IAA in the multiplication medium was detrimental to shoot proliferation, shoot growth was not affected by IAA. The production of large amounts of basal callus and vitrification of shoots were the major problems to be avoided in proliferating shoot cultures. Addition of TIBA to the multiplication medium markedly reduced basal callusing, while sealing the culture vessels with a fluorocarbon polymer (tetrafluoroethyleneperfluoroalkyl vinyl ether) film (Neoflon PFA film) almost completely eliminated vitrification. A reduction in the number of vitrified shoots was also achieved with AVG treatment. Following this protocol of using BA-supplemented WPM and Neoflon film, it would be possible to produce more than 100,000 plants from a single stem cutting in 1 year.Abbreviations
AVG
Aminoethoxyvinylglycine
-
BA
N6-benzyladenine
-
BM
basal medium
-
IAA
indole-3-acetic acid
-
IBA
indole-3-butyric acid
-
2-tiP
N6-(2-isopentenyl)adenine
-
KIN
kinetin
-
MS
Murashige and Skoog medium
-
NAA
-naphthaleneacetic acid
-
SRM
shoot regeneration medium
-
TDZ
thidiazuron
-
TIBA
2,3,5-triiodobenzoic acid
-
WPM
woody plant medium
-
ZEA
zeatin 相似文献
7.
Sarah A. Wilson Patricia Keen Michelle C. McKee Nicole Raia Joyce Van Eck Susan C. Roberts 《In vitro cellular & developmental biology. Plant》2018,54(1):36-44
The FDA-approved anti-cancer compound paclitaxel is currently produced commercially by Taxus plant cell suspension cultures. One major limitation to the use of plant cell culture as a production platform is the low and variable product yields. Therefore, methods to increase and stabilize paclitaxel production are necessary to ensure product security, especially as the demand for paclitaxel continues to rise. Although a stable transformation method for Taxus suspension cultures has been developed, stable transformant yields are low (around 1% of experiments) and the method does not translate to the Taxus cuspidata Siebold and Zucc. and Taxus canadensis Marshall cell lines utilized in this study. Therefore, a new method for Agrobacterium-mediated transformation of Taxus callus and suspension cultures was developed through identification of the optimal Agrobacterium strain, inclusion of an anti-necrotic cocktail (silver nitrate, cysteine, and ascorbic acid) and increased recovery time for cells after cocultivation, the time following infection with Agrobacterium tumefaciens. Application of the increased recovery time to transformation of T. cuspidata line PO93XC resulted in 200 calluses staining positive for GUS. Additionally, two transgenic lines have been maintained with stable transgene expression for over 5 yr. This method represents an improvement over existing transformation methods for Taxus cultures and can be applied for future metabolic engineering efforts. 相似文献
8.
Improved paclitaxel production by in situ extraction and elicitation in cell suspension cultures of Taxus chinensis 总被引:2,自引:0,他引:2
Dibutyl phthalate, oleic acid and terpineol were used to extract paclitaxel in situ fromTaxus chinensis suspension cultures. Oleic acid/terpineol (1:1, v/v) added to the cultures gave a higher paclitaxel concentration, compared with either of them alone. Oleic acid/terpineol (1:1, v/v) incorporated into the cultures at 3:50 (v/v) 4 days after elicitation, which was carried out by adding 50 mg chitosan l–1, 60 M methyl jasmonate and 30 M Ag+ to 10-day-old cultures, resulted in the greatest paclitaxel production of 48 mg l–1 at day 10 after elicitation. This was double that of the culture by elicitation, and 7-fold higher than that of the culture by in situ extraction. 相似文献
9.
Valeriana glechomifolia is a plant species endemic to southern Brazil that accumulates valepotriates, which are terpene derivatives, in all of its organs. Valepotriates are the presumed sedative generic components of the pharmaceutically used species of Valeriana. The influence of various concentrations of the auxins indole-3-acetic acid, indole-3-butyric acid and -naphthaleneacetic acid on the growth of micropropagated V. glechomifolia was investigated under conditions of transient and continuous exposure. Changes in the development of roots and shoots as well as the production of the valepotriates acevaltrate, valtrate and didrovaltrate (analyzed by high-performance liquid chromatography) were evaluated. The best performance in valepotriate production, growth and survival under ex vitro conditions following plant acclimatization was achieved in the continuous presence of 5.71 M IAA. When cultured in medium containing IAA plants produced stable levels of valepotriates throughout the entire cultivation period.Abbreviations ACE
Acevaltrate
- IAA
Indole-3-acetic acid
- IBA
Indole-3-butyric acid
- DW
Dry weight
- DID
Didrovaltrate
- NAA
-Naphthaleneacetic acid
- PVP
Polyvinylpyrrolidone
- VAL
Valtrate 相似文献
10.
Agrobacterium-mediated transformation of hybrid poplar suspension cultures and regeneration of transformed plants 总被引:6,自引:0,他引:6
Glenn T. Howe Barry Goldfarb Steven H. Strauss 《Plant Cell, Tissue and Organ Culture》1994,36(1):59-71
A method for Agrobacterium-mediated transformation of hybrid poplar (Populus alba x P. grandidentata cv. Crandon) suspension cultures and regeneration of transformed plants is described. Transformants were recovered when suspension cultures were inoculated with Agrobacterium tumefaciens at a density of 107 colony-forming units ml-1, cocultivated for 48 h, and plated to cellulose acetate filters on Woody Plant Medium containing 4.5 M 2,4-dichlorophenoxyacetic acid and 250 mg l-1 cefotaxime. Levels of cefotaxime greater than 250 mg l-1 were unnecessary for control of residual bacteria and inhibited callus growth. Transgenic plants were regenerated by culturing the transformed callus on media containing 0.11 to 27 M thidiazuron. In contrast to thidiazuron, N6-benzyladenine had a negative effect on shoot regeneration; the callus became necrotic when we attempted to induce shoots with concentrations of 1.1 to 8.9 M, and growth was inhibited when concentrations of 0.11 or 0.22 M were used to regenerate callus from suspension cultures. Following cocultivation of poplar suspension cultures, we recovered transgenic plants containing the maize transposon Ac, and callus containing an insect toxin gene from Bacillus thuringiensis.Abbreviations BA
N6-benzyladenine
- CIM
callus initiation medium
- CaMV
cauliflower mosaic virus
- cfu's
colony-forming units
- HPT
hygromycin phosphotransferase
- MS
Murashige and Skoog medium (Murashige & Skoog 1962)
- NPT-II
neomycin phosphotransferase-II
- PAR
photosynthetically active radiation
- PCR
polymerase-chain-reaction
- TDZ
thidiazuron
- WPM
Woody Plant Medium (Lloyd & McCown 1980)
- 2,4-d
2,4-dichlorophenoxyacetic acid 相似文献
11.
5‐Aminolevulinic acid promotes callus growth and paclitaxel production in light‐grown Taxus cuspidata suspension cultures 下载免费PDF全文
Shinjiro Yamamoto Shuhei Hayashi Shintaro Furusaki Suteaki Shioya 《Engineering in Life Science》2015,15(1):116-121
Cultured plant cells generally produce low levels of secondary metabolites, and elicitors of secondary metabolites usually inhibit callus growth. The aim of this study was to determine the effect of 5‐aminolevulinic acid (ALA), a chlorophyll precursor that promotes plant growth, on callus induction from leaves of Taxus cuspidata, and on callus growth on solid medium. ALA at 0.76, 7.6, and 76 μM had similar effects on callus induction and growth, while ALA at 760 μM had negative effects. Next, the effects of ALA concentrations on callus growth and paclitaxel production in suspension cultures in the dark were evaluated. The results showed that 0.76 and 7.6 μM ALA stimulated growth and paclitaxel production, while 76 μM ALA had negative effects. ALA is thought to promote cellular activity under light conditions. Therefore, the effects of light intensity on callus growth and paclitaxel production in the presence of ALA were evaluated. Our results showed that the best conditions for callus growth and paclitaxel production were 7.6 μM ALA under photosynthetically active radiation of 12 μmol photons m?2 s?1. Callus growth and paclitaxel production were inhibited under stronger light (24 μmol photons m?2 s?1). Together, these results show that ALA promoted callus growth and the production of paclitaxel by light‐grown cultured T. cuspidata cells. 相似文献
12.
Somatic embryogenesis and callus production from cotyledon explants of Eastern black walnut 总被引:4,自引:0,他引:4
Mark C. Neuman John E. Preece J. W. Van Sambeek Gerald R. Gaffney 《Plant Cell, Tissue and Organ Culture》1993,32(1):9-18
Starting at 8 weeks and continuing until 23 weeks (nut drop) after anthesis,1 m2 explants from cotyledons of immature seeds were extracted from Juglans nigra fruits. Explants were placed on Woody Plant Medium with 1 g l-1 casein hydrolysate and 30 g l-1 sucrose. The explants remained in light for 4 weeks on primary media containing a 3×3 factorial of 0.05, 0.5, or 5.0 M thidiazuron (TDZ) and 0.1, 1.0, or 10.0 M 2,4-d. Explants were transferred to a secondary medium containing no plant growth regulators and incubated in darkness for 11 weeks. The greatest number of somatic embryos was produced 8, 10, and 12 weeks after anthesis from explants on media with 0.5 or 5.0 M TDZ and 0.1 or 1.0 M 2,4-d. Explants produced the greatest callus volume and dry weight 10, 12, and 14 weeks after anthesis. Throughout the study, callus generally increased with increasing concentrations of both TDZ and 2,4-d.Abbreviations BA
6-benzyladenine
- captan
3a,4,7,7a-tetrahydro-2-[(trichloromethyl)thio]-1H-isoindole-1,3(2H)-dione
- 2,4-d
2,4-dichlorophenoxyacetic acid
- IBA
indolebutyric acid
- Physan
n-alkyl- dimethyl-benzyl ammonium chlorides and n-alkyl-dimethyl-ethylbenzyl ammonium chlorides
- TDZ-thidiazuron
N-phenyl-N-1,2,3-thiadiazol-5-ylurea 相似文献
13.
Wayne A. Mackay Jimmy L. Tipton Gary A. Thompson 《Plant Cell, Tissue and Organ Culture》1995,43(3):295-299
Mexican redbud (Cercis canadensis var. mexicana) shoot cultures were initiated from explants taken from both mature and juvenile stock plants. Culture conditions affecting shoot growth and proliferation and rooting of three clones were investigated. Shoot growth was best on media supplemented with 0.25% activated charcoal and solidified with 0.2% Gelrite. Four commercially available salt formulations (Anderson's rhododendron medium, WPM, MS, DKW) were tested for growth of shoot cultures, and Anderson's rhododendron basal salt mixture was superior. Axillary shoots grew from explants cultured media supplemented with a wide range of concentrations of benzyladenine and thidiazuron. Benzyladenine at 5.6–22.2 M supported the best combination of shoot quality and number. Rooting of microshoots in vitro was best on half-strength WPM containing 6.71 M naphthaleneacetic acid and 0.1% activated charcoal.Abbreviations BA
6-benzyladenine
- IBA
indole-3-butyric acid
- 2iP
6-(, -dimethylallylamino)purine
- DKW
Driver & Kuniyuki Walnut
- kinetin
6-furfurlaminopurine
- MS
Murashige & Skoog
- NAA
-naphthaleneacetic acid
- WPM
Woody Plant Medium
- TDZ
thidiazuron
- 1-phenyl-3
(1,2,3-thidiazol-5-yl)urea 相似文献
14.
Massimo H. M. Sanago Vern I. Shattuck Judith Strommer 《Plant Cell, Tissue and Organ Culture》1996,45(2):165-168
A simple and rapid pea regeneration procedure was developed. An average of up to 20 shoots formed from hypocotyl explants of cvs. Sugar Ann and Patriot cultured on Murashige and Skoog basal medium supplemented with 0.5 or 1.0 M thidiazuron (N-phenyl-N-1,2,3-thiadiazol-5-ylurea). Hypocotyls of Puget and Sugar Daddy did not respond. Regenerated shoots rooted rapidly when cultured on Murashige and Skoog basal medium containing either 2.0 M -naphthaleneacetic acid or 1.0–2.0 M indole-3-butyric acid. Seeds were harvested from regenerated plants after only 9–11 weeks.Abbreviations BAP
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxy acetic acid
- GA3
gibberellic acid
- IBA
indole-3-butyric acid
- MS
Murashige and Skoog (1962) medium
- NAA
-naphthaleneacetic acid
- TDZ
thidiazuron (N-phenyl-N-1,2,3-thiadiazol-5-ylurea) 相似文献
15.
Different combinations of auxins and cytokinins were employed to assess the regeneration capacity from in vitro leaf explants of Lonicera nitida Wils. cv Maïgrün. A high frequency of rhizogenesis was noticed, with 2.3 M thidiazuron plus 2.9 M indole-3-acetic acid as the only hormonal combination to support caulogenic responses. Increasing thidiazuron concentration and/or suppressing auxin did not improve caulogenesis. Combining thidiazuron with 2,3,5-triiodobenzoic acid produced a dramatic increase in the percentage of caulogenic explants. A maximum of 74% of adventitious bud forming explants was obtained with 2.3 M thidiazuron plus 20 M 2,3,5-triiodobenzoic acid. Buds were often in a rosette form and were vitreous, so that shoot elongation was difficult to obtain. The effect of the duration of the 2,3,5-triiodobenzoic acid treatment on shoot elongation was investigated.Abbreviations BAP
benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- 2 IP
2-isopentenyladenine
- MS
Murashige and Skoog
- NAA
-naphthylacetic acid
- TDZ
N-phenyl-N-1,2,3-thidiazol-5-ylurea (thidiazuron)
- TIBA
2,3,5-triiodobenzoic acid
- Z
zeatin 相似文献
16.
Oxindole-3-acetic acid (OxIAA) has been identified in germinating seeds of Scots pine (Pinus sylvestris) using gas chromatography-mass spectrometry. Seeds germinated for 5 d contained 2.7 ng OxIAA·g-1 (dry weight) whereas ungerminated seeds contained 0.2 ng·g-1. Isotopically labelled OxIAA was formed in seeds incubated with [1-14C]-, [2-14C]- or [2H5]indole-3-acetic acid.Abbreviations DDC
sodium diethyldithiocarbamate
- GC
gas chromatography
- HPLC
high-performance liquid chromatography
- IAA
indole-3-acetic acid
- MS
mass spectrometry
- OxIAA
oxindole-3-acetic acid
- PVP
polyvinylpyrrolidone
- TMS
trimethylsilyl 相似文献
17.
M. Furmanowa H. Olędzka K. Sykłowska-Baranek J. Józefowicz S. Gieracka 《Biotechnology letters》2000,22(18):1449-1452
In Taxus cuspidata callus, vanadyl sulfate (10 mg l–1) induced a high (146 g g–1 dry wt) production of 10-deacetylbaccatin III in comparison to 7 g g–1 dry wt of the control. The content of paclitaxel in this species increased from 16 g g–1 to 74 g g–1 dry wt when 20 mg phenylalanine l–1 was used. In T. media, p-aminobenzoic acid induced the highest content of 10-deacetylbaccatin III (481 g g–1 dry wt) versus 181 g g–1 in the control. Paclitaxel increased from 89 to 139 g g–1 dry wt after adding chitosan (20 mg l–1) to the cultures. 相似文献
18.
A structured kinetic model for suspension cultures of Taxus chinensis var. mairei induced by an oligosaccharide from Fusarium oxysporum 总被引:1,自引:0,他引:1
A structured kinetic model was established to describe the process of Taxol formation in suspension cultures of Taxus chinesis var. mairei induced by an oligosaccharide from Fusarium oxysporum. In this model, the role of intracellular starch as a storage carbon source had to be taken into account. Substrate uptake, culture growth, cell respiration, and secondary metabolites, predicted by the model, agreed with those obtained experimentally. The effective factors of oligosaccharide elicitation, e,j, defined as the ratio of the parameter values in the system with oligosaccharide to those in control, reflected the effects of the oligosaccharide on cell growth and Taxol production. 相似文献
19.
Apoptotic cell death in suspension cultures of Taxus cuspidata induced by exogenous salicylic acid and/or H2O2 was investigated. H2O2 (0.012% v/v) alone changed the permeability of cell membrane while salicylic acid (0.375 mM) not only altered the permeability but also caused nuclei condensation and a small amount of nuclei fragments. The combined use of salicylic acid (0.375 mM) and H2O2 (0.012% v/v) changed the cell membrane permeability more significantly and nuclei fragments occurred in ca. 30% of the cells at 48 h. DNA ladders of 180 bp and oligopolymers, characteristics of the apoptotic cleavage of nuclei DNA, were observed by agar electrophoresis. These results show that exogenous salicylic acid and H2O2 could synergistically induce the apoptotic cell death of suspension cultures of Taxus cuspidata. 相似文献
20.
Atriplex gmelini plants were regenerated via organogensis from hypocotyl explants. Callus lines were induced from the hypocotyl explants on Linsmaier and Skoog (LS) medium supplemented with 1 M benzyladenine and 5 M -naphthaleneacetic acid in the dark. Shoots were regenerated from the callus lines on LS medium supplemented with 20 M thidiazuron and 0.1 M -naphthaleneacetic acid under a high-intensity light condition (450 mol m–2 s–1). The regenerated shoots were rooted on LS medium without growth regulators to obtain fully developed plants. We succeeded in transforming Atriplex gmelini from callus lines using Agrobacterium tumefaciens. 相似文献