The introduction of metagenomics into an undergraduate biochemistry laboratory course yielded a predicted reductase that decreases triclosan susceptibility in Escherichia coli

Traditional undergraduate science classes often include a laboratory component aimed at enabling the students to experience the classroom topics firsthand. Typically, these experiments are chosen because they have known outcomes that will clearly demonstrate particular aspects of scientific theory. While this approach has its benefits in skill development and concept reinforcement, the lack of novelty inherent in repeating experiments that have been repeated for many years does not accurately convey the feeling of true scientific discovery to the students. In this work, we have designed and implemented a series of experiments into an undergraduate biochemistry curriculum that incorporates the opportunity for scientific discovery, while simultaneously creating an environment for learning routine laboratory techniques. Through this set of experiments, students enrolled in the course were successful in identifying and beginning to characterize an unknown bacterial gene that confers increased tolerance to triclosan on its host.     

Problem-oriented small-group discussion in the teaching of biochemistry laboratory practicals

An experiment on the use of problem-based exercises in an Indian Medical School is described. Problem-based classes were supplemented with laboratory classes and feedback from both students and tutors was analyzed.     

Computerised data acquisition and analysis in the biochemistry teaching laboratory

We have successfully implemented a simple computerised data acquisition system which has been used extensively in our biochemistry teaching laboratory classes. Our experience suggests that it is important to consider carefully what the computers are required to do in the laboratory well before their introduction. Finally, we believe that it is desirable to keep the system simple and sufficiently versatile that it can be adapted to several different uses without too much difficulty.     

Biodegradation as a biotechnological model for the teaching of biochemistry

Review: Biodegradation as a biotechnological model for the teaching of biochemistry. A knowledge of waste treatment and the biodegradation processes involved is necessary for undergraduates in agriculture, chemistry, biology, food technology, etc. Courses in these subjects must make adequate provision for such instruction. In this article, we suggest a theoretical and practical study of composting, which stimulates the interest of the students in metabolic pathways involved in this, and other, biotechnological processes.     

Using Xenopus as a model system for an undergraduate laboratory course in vertebrate development at the University of Bordeaux,France

The goal of this laboratory course is to introduce vertebrate developmental biology to undergraduate students, emphasizing both classical and contemporary aspects of this field. During the course, the students combine the use of living Xenopus laevis material with active tutorial participation, with the aim of illustrating how the fertilized egg can generate the diversity of cell types and complexity of pattern seen only a few days later in the embryo. Special emphasis is given to the observation and manipulation of living material. The laboratory course includes a comprehensive analysis of both oogenesis and early development and is divided into two overlapping parts that combine tutorial and practical approaches. The first part is devoted to oogenesis; oocytes are sorted out, allowed to mature in vitro and observed in histological section. In the second part, students perform an in vitro fertilization of Xenopus eggs and a mesoderm and neural induction assay of animal cap explants. Successful induction of the explants is confirmed by morphological, histological and molecular analyses. Finally, the students observe and comment on selected slides to illustrate the organization of the body plan of the amphibian embryo at an early stage of organogenesis.     

'Fiber 7' supplement as an alternative to laxatives in a nursing home

Background: Chronic constipation is a common and bothersome problem in elderly nursing home patients. Although fibre has a known role in the treatment of constipation, laxatives are still widely used in nursing home patients. Objective: To determine whether the addition of a fibre supplement to oral diet can reduce the use of laxatives in a long‐term care facility. Participants and methods: Oral diet of 92 nursing home residents (all older than 65) on chronic laxatives (docusate, milk of magnesia with Cascara, psyllium) was supplemented with ‘Fiber 7’, a natural powder fibre (7 g per meal twice a day). During the next 2.5 years discontinuation of laxatives was attempted. Results: Laxatives were successfully discontinued in 63 of the 92 patients (68.5%, 95% confidence interval 59–78%). The fibre supplement was well tolerated. The cost of care was decreased by $3.5 per patient every month or by $220/month for the 63 patients. Conclusions: Use of ‘Fiber 7’ allowed discontinuation of laxatives in 63 of 92 nursing home residents. The fibre supplement was a safe and convenient alternative to laxatives and decreased the cost of medical care.     

L-methionine as immune supportive supplement: a clinical evaluation

Summary. The objective of the study was to test L-methioinine as a possible immune supportive supplement in HIV infected patients by means of a clinical study. A double-blind, placebo-controlled study was designed. The patients (n = 253) from four different trial centres were randomly divided into two groups, active and placebo, and regularly assessed by clinical and safety parameters. After six months from commencement, clinically and statistically significant differences were observed. The females of the active treatment group presented with a decreased level of decline in their CD4 counts (p = 0.0027), so also the patients of Centre 1 (p = 0.0377). All patients were placed onto active treatment after 12 months and were followed up for 48 months after the trial started. The same tendencies could be observed in the group as a whole, with no serious side effects directly associated to treatment. The study confirmed the supportive role of L-methionine in immune-compromised or deficient patients.     

A survey of the state of the art of clinical biochemistry laboratory computerisation

This paper presents the results of a survey which was recently carried out in order to clarify the current ‘state of the art’ regarding the use of computers in hospital biochemistry laboratories. Information was obtained by means of a questionnaire which was mailed to a sample of United Kingdom hospital laboratories. The results obtained from the analysis of the data from the questionnaire replies are presented, and conclusions are drawn regarding the implications of hospital laboratory computerisation.