共查询到20条相似文献,搜索用时 31 毫秒
1.
M. Raydan N. A. Shubin M. I. Blinova G. G. Prokhorov G. P. Pinaev 《Cell and Tissue Biology》2010,4(6):529-538
The goal of this study was to conduct a comparative analysis of the degree of resistance to low temperatures of human epidermal
cells found at different stages of differentiation. The action of liquid-nitrogen vapors was analyzed in experiments in vitro
at various temperature regimes on fragments of the human integral skin and on isolated from them and cultivated keratinocytes.
The degree of resistance of keratinocytes to the action of cooling to low temperatures was evaluated by their ability to form
a multilayer stratum in culture, which indicates the preservation of the viability of the cells treated with cold. This approach
allowed one to reveal the diapason of optimal regimes of the action of low temperatures on cells in the composition of tissue
and after their conversion into culture. The quantitative ratios of the epidermal stem, transitory, and differentiated cells
in a population of viable cells before and after exposure to low temperatures were determined with antibodies that correspond
to their different stages of differentiation. The degree of resistance of keratinocytes to action of cooling to low temperatures
was evaluated by their ability to form a multilayer stratum in culture, which indicates preservation of viability of the cells
treated with cold. The results of this study show that the resistance of human epidermal cells to low temperature differs
depending on their stage of differentiation both in situ and in vitro. The epidermal stem and transitory cells are more stable
than the differentiated cells. 相似文献
2.
Thomas Tilling Ewa Wladykowski Antonio Virgilio Failla Pia Houdek Johanna M. Brandner Ingrid Moll 《Histochemistry and cell biology》2014,141(4):407-421
Merkel cells, the neurosecretory cells of skin, are essential for light-touch responses and may probably fulfill additional functions. Whether these cells derive from an epidermal or a neural lineage has been a matter of dispute for a long time. In mice, recent studies have clearly demonstrated an epidermal origin of Merkel cells. Given the differences in Merkel cell distribution between human and murine skin, it is, however, unclear whether the same holds true for human Merkel cells. We therefore attempted to gain insight into the human Merkel cell lineage by co-immunodetection of the Merkel cell marker protein cytokeratin 20 (CK20) with various proteins known to be expressed either in epidermal or in neural stem cells of the skin. Neither Sox10 nor Pax3, both established markers of the neural crest lineage, exhibited any cell co-labeling with CK20. By contrast, β1 integrin, known to be enriched in epidermal stem cells, was found in nearly 70 % of interfollicular epidermal and 25 % of follicular Merkel cells. Moreover, LRIG1, also enriched in epidermal stem cells, displayed significant co-immunolabeling with CK20 as well (approximately 20 % in the interfollicular epidermis and 7 % in the hair follicle, respectively). Further epidermal markers were detected in sporadic Merkel cells. Cells co-expressing CK20 with epidermal markers may represent a transitory state between stem cells and differentiated cells. β1 integrin is probably also synthesized by a large subset of mature Merkel cells. Summarizing, our data suggest that human Merkel cells may originate from epidermal rather than neural progenitors. 相似文献
3.
Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells
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He LiJuan Nan Xue Wang YunFang Guan LiDong Bai CiXian Shi ShuangShuang Yuan HongFeng Chen Lin Liu DaQing Pei XueTao 《中国科学C辑(英文版)》2007,50(4):429-437
To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesenchymal stem cells (BMSCs)
were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissueengineered skin. The results
showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle
shape to round or oval; tonofibrils, melanosomes and keratohyaline granules were observed under a transmission electronic
microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% ± 4.2%) and epidermal cells
differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered
apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission
electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in
differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06
pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material shortened skin wound repair significantly. In one word, autogenic BMSCs have the
potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering. 相似文献
4.
HE LiJuan NAN Xue WANG YunFang GUAN LiDong BAI CiXian SHI ShuangShuang YUAN HongFeng CHEN Lin LIU DaQing & PEI XueTao 《中国科学:生命科学英文版》2007,50(4):429-437
To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesenchymal stem cells (BMSCs)
were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissueengineered skin. The results
showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle
shape to round or oval; tonofibrils, melanosomes and keratohyaline granules were observed under a transmission electronic
microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% ± 4.2%) and epidermal cells
differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered
apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission
electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in
differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06
pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material shortened skin wound repair significantly. In one word, autogenic BMSCs have the
potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering.
Supported by the Major Technology Program of Beijing Municipal Science & Technology Commission (Grant No. H060920050130) and
the Major State Basic Research Development Program of China (Grant No. 2005CB522702) 相似文献
5.
《The Journal of cell biology》1996,135(6):1879-1887
The Distal-less-related homeodomain gene Dlx3 is expressed in terminally differentiated murine epidermal cells. Ectopic expression of this gene in the basal cell layer of transgenic skin results in a severely abnormal epidermal phenotype and leads to perinatal lethality. The basal cells of affected mice ceased to proliferate, and expressed the profilaggrin and loricrin genes which are normally transcribed only in the latest stages of epidermal differentiation. All suprabasal cell types were diminished and the stratum corneum was reduced to a single layer. These data indicate that Dlx3 misexpression results in transformation of basal cells into more differentiated keratinocytes, suggesting that this homeoprotein is an important regulator of epidermal differentiation. 相似文献
6.
Sabine Nagel Franziska Rohr Caroline Weber Janina Kier Frank Siemers Charli Kruse Sandra Danner Matthias Brandenburger Anna Emilia Matthiessen 《PloS one》2013,8(10)
Human skin harbours multiple different stem cell populations. In contrast to the relatively well-characterized niches of epidermal and hair follicle stem cells, the localization and niches of stem cells in other human skin compartments are as yet insufficiently investigated. Previously, we had shown in a pilot study that human sweat gland stroma contains Nestin-positive stem cells. Isolated sweat gland stroma-derived stem cells (SGSCs) proliferated in vitro and expressed Nestin in 80% of the cells. In this study, we were able to determine the precise localization of Nestin-positive cells in both eccrine and apocrine sweat glands of human axillary skin. We established a reproducible isolation procedure and characterized the spontaneous, long-lasting multipotent differentiation capacity of SGSCs. Thereby, a pronounced ectodermal differentiation was observed. Moreover, the secretion of prominent cytokines demonstrated the immunological potential of SGSCs. The comparison to human adult epidermal stem cells (EpiSCs) and bone marrow stem cells (BMSCs) revealed differences in protein expression and differentiation capacity. Furthermore, we found a coexpression of the stem cell markers Nestin and Iα6 within SGSCs and human sweat gland stroma. In conclusion the initial results of the pilot study were confirmed, indicating that human sweat glands are a new source of unique stem cells with multilineage differentiation potential, high proliferation capacity and remarkable self renewal. With regard to the easy accessibility of skin tissue biopsies, an autologous application of SGSCs in clinical therapies appears promising. 相似文献
7.
Jia L Zhou J Peng S Li J Cao Y Duan E 《Biochemical and biophysical research communications》2008,368(3):483-488
Epidermal stem cells maintain development and homeostasis of mammalian epidermis throughout life. However, the molecular mechanisms involved in the proliferation and differentiation of epidermal stem cells are far from clear. In this study, we investigated the effects of Wnt3a and Wnt/β-catenin signaling on proliferation and differentiation of human fetal epidermal stem cells. We found both Wnt3a and active β-catenin, two key members of the Wnt/β-catenin signaling, were expressed in human fetal epidermis and epidermal stem cells. In addition, Wnt3a protein can promote proliferation and inhibit differentiation of epidermal stem cells in vitro culture. Our results suggest that Wnt/β-catenin signaling plays important roles in human fetal skin development and homeostasis, which also provide new insights on the molecular mechanisms of oncogenesis in human epidermis. 相似文献
8.
Isolation of human basal keratinocytes by selective adhesion to extracellular matrix proteins 总被引:1,自引:0,他引:1
Spichkina OG Kalmykova NV Kukhareva LV Voronkina IV Blinova MI Pinaev GP 《Tsitologiia》2006,48(10):841-847
Epidermal human cells (keratinocytes) differently interact with extracellular matrix proteins of the skin basal membrane depending on the stages of their differentiation. The pool of basal keratinocytes commonly includes stem cells and transient amplifying cells. They directly attach to the skin basal membrane. Keratinocytes change their adhesive properties during differentiation, lose direct interaction with the basal membrane and move to suprabasal epidermal strata. From this, it is suggested that basal and primarily stem cells can be isolated from a heterogenous keratinocyte population due to their selective adhesion to the extracellular matrix proteins. In the current study, we analysed the specificity of interaction between primary keratinocytes and extracellular matrix proteins (collagens of I and IV types, laminin-2/4, fibronectin and matrigel). We have demonstrated that the basal keratinocytes extracted from the skin have different adhesive abilities. The rapidly spreading cells usually interacted with collagen and fibronectin rather that with laminin-2/4 or matrigel. The majority of these cells being represented by basal keratinocytes. Our data demonstrate that the applied method of keratinocyte selection may be directed for precise isolation of skin stem from a common cell population. 相似文献
9.
Embryonic stem cells as a cellular model for neuroectodermal commitment and skin formation 总被引:1,自引:0,他引:1
Aberdam D Gambaro K Medawar A Aberdam E Rostagno P de la Forest Divonne S Rouleau M 《Comptes rendus biologies》2007,330(6-7):479-484
Embryonic stem (ES) cells can be differentiated into many cell types in vitro, thus providing a potential unlimited supply of cells for cognitive in vitro studies and cell-based therapy. We recently reported the efficient derivation of ectodermal and epidermal cells from murine ES cells. These differentiated ES cells were able to form, in culture, a multilayered epidermis coupled with an underlying dermal compartment, similar to native skin. We clarified the function of BMP-4 in the binary neuroectodermal choice by stimulating sox-1(+) neural precursors to undergo specific apoptosis while inducing epidermal differentiation through DeltaNp63 gene activation. We further demonstrated that DeltaNp63 enhances ES-derived ectodermal cell proliferation and is necessary for epidermal commitment. This unique cellular model further provides a powerful tool for identifying the molecular mechanisms controlling normal skin development and for investigating p63-ectodermal dysplasia human congenital pathologies. 相似文献
10.
Maganga R Giles N Adcroft K Unni A Keeney D Wood F Fear M Dharmarajan A 《Biochemical and biophysical research communications》2008,377(2):606-611
The skin provides vital protection from infection and dehydration. Maintenance of the skin is through a constant program of proliferation, differentiation and apoptosis of epidermal cells, whereby proliferating cells in the basal layer differentiating to form the keratinized, anucleated stratum corneum. The WNT signalling pathway is known to be important in the skin. WNT signalling has been shown to be important both in epidermal development and in the maintenance and cycling of hair follicles and epidermal stem cells. However, the precise role for this pathway in epidermal differentiation remains unknown.We investigated the role of the WNT signalling inhibitor sFRP4 in epidermal differentiation. sFRP4 is expressed in both normal skin and keratinocytes in culture. Expression of sFRP4 mRNA and protein increases with keratinocyte differentiation and apoptosis, whilst exposure of keratinocytes to exogenous sFRP4 promotes apoptosis and expression of the terminal differentiation marker Involucrin.These data suggest sFRP4 promotes epidermal differentiation. 相似文献
11.
12.
The location and identity of interfollicular epidermal stem cells of adult human skin remain undefined. Based on our previous work in both adult murine and neonatal human foreskin, we demonstrate that cell surface levels of the alpha6 integrin and the transferrin receptor (CD71) are valid markers for resolving a putative stem cell, transit amplifying and differentiating compartment in adult human skin by flow cytometry. Specifically, epidermal cells expressing high levels of alpha6 integrin and low levels of the transferrin receptor CD71 (phenotype alpha6 (bri)CD71(dim)) exhibit several stem cell characteristics, comprising a minor population (2%-5%) of the K14(bri) fraction, enriched for quiescent and small blast-like cells with high clonogenic capacity, lacking the differentiation marker K10. Conversely, the majority of K14(bri) K10(neg) epidermal cells express high levels of CD71 (phenotype alpha6 (bri)CD71(bri)), and represent the actively cycling fraction of keratinocytes displaying greater cell size due to an increase in cytoplasmic area, consistent with their being transient amplifying cells. The alpha6 (bri)CD71(bri) population exhibited intermediate clonogenic capacity. A third population of K14(dim) but K10 positive epidermal cells could be identified by their low levels of alpha6 integrin expression (i.e. alpha6 (dim) cells), representing the differentiation compartment; predictably, this subpopulation exhibited poor clonogenic efficiency. Flow cytometric analysis for the hair follicle bulge region (stem cell) marker K15 revealed preferential expression of this keratin in alpha6 (bri) cells (i.e., both stem and transient amplifying fractions), but not the alpha6 (dim) population. Given that K15 positive cells could only be detected in the deep rete ridges of adult skin in situ, we conclude that stem and transient amplifying cells reside in this location, while differentiating (K15 negative) cells are found in the shallow rete ridges. 相似文献
13.
14.
Multi-potentiality of a new immortalized epithelial stem cell line derived from human hair follicles 总被引:2,自引:0,他引:2
Roh C Roche M Guo Z Photopoulos C Tao Q Lyle S 《In vitro cellular & developmental biology. Animal》2008,44(7):236-244
We previously demonstrated that keratin 15 expressing cells present in the bulge region of hair follicles exhibit properties of adult stem cells. We have now established and characterized an immortalized adult epithelial stem cell line derived from cells isolated from the human hair follicle bulge region. Telogen hair follicles from human skin were microdissected to obtain an enriched population of keratin 15 positive skin stem cells. By expressing human papillomavirus 16 E6/E7 genes in these stem cells, we have been able to culture the cells for >30 passages and maintain a stable phenotype after 12 mo of continuous passage. The cell line was compared to primary stem cells for expression of stem cell specific proteins, for in vitro stem cell properties, and for their capacity to differentiate into different cell lineages. This new cell line, named Tel-E6E7 showed similar expression patterns to normal skin stem cells and maintained in vitro properties of stem cells. The cells can differentiate into epidermal, sebaceous gland, and hair follicle lineages. Intact beta-catenin dependent signaling, which is known to control in vivo hair differentiation in rodents, is maintained in this cell line. The Tel-E6E7 cell line may provide the basis for valid, reproducible in vitro models for studies on stem cell lineage determination and differentiation. 相似文献
15.
低温对小菜蛾实验种群的影响 总被引:1,自引:3,他引:1
研究了低温(<8℃)对小菜蛾的发育、存活和繁殖的影响结果表明,卵和蛹在4℃和6℃下死亡率随处理时间的延长而增加,在分别处理55d和70d后,卵和蛹全部死亡;经4℃和6℃处理的蛹,在16℃下羽化成虫的平均产卵量随处理时间的延长而减少,处理45d时,产卵量均为0小菜蛾幼期各虫态在0℃以下,死亡率随低温强度加大和处理时问的延长而增高就耐寒力而言,3龄幼虫和蛹最强,其次是2龄和4龄幼虫,卵和1龄幼虫的耐寒力最弱不同低温和时间处理小菜蛾幼期虫态对其后继虫态的发育历期有较大影响,总体说来,经过处理的小菜蛾幼期虫态,其后继虫态的发育历期普遍延长,一般处理某一虫态对其相邻虫态发育历期的影响最大小菜蛾蛹经低温处理后其羽化成虫的产卵量随着蛹期所经历低温强度的增强和时间延长而减少。 相似文献
16.
Lei XH Ning LN Cao YJ Liu S Zhang SB Qiu ZF Hu HM Zhang HS Liu S Duan EK 《PloS one》2011,6(11):e26603
The skin is susceptible to different injuries and diseases. One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as a "stimulatory" environment for the proliferation and differentiation of stem cells. Here, we employed the NASA-approved rotary bioreactor to investigate the proliferation and differentiation of human epidermal stem cells (hEpSCs). hEpSCs were isolated from children foreskins and enriched by collecting epidermal stem cell colonies. Cytodex-3 micro-carriers and hEpSCs were co-cultured in the rotary bioreactor and 6-well dish for 15 days. The result showed that hEpSCs cultured in rotary bioreactor exhibited enhanced proliferation and viability surpassing those cultured in static conditions. Additionally, immunostaining analysis confirmed higher percentage of ki67 positive cells in rotary bioreactor compared with the static culture. In contrast, comparing with static culture, cells in the rotary bioreactor displayed a low expression of involucrin at day 10. Histological analysis revealed that cells cultured in rotary bioreactor aggregated on the micro-carriers and formed multilayer 3D epidermis structures. In conclusion, our research suggests that NASA-approved rotary bioreactor can support the proliferation of hEpSCs and provide a strategy to form multilayer epidermis structure. 相似文献
17.
Patterns of nestin expression in human skin 总被引:7,自引:0,他引:7
18.
Hair follicle stem cells play important roles in maintaining homeostasis and skin tissue self-renewal. Transit-amplifying cells represent the transition of cells from hair follicle stem cells into differentiated epidermal cells. Thus far, the signaling pathway and the molecular biological mechanism that regulate the proliferation and differentiation of hair follicle stem cells remain unclear. In this paper, we studied the relationship between β-catenin and c-myc during the process of the differentiation of hair follicle stem cells into transit-amplifying cells. Based on our results, the expression of β-catenin can activate the nuclear gene c-myc and regulate the expression of transit-amplifying cell markers K15, K19, a6-integrin and β1-integrin, indicating that β-catenin is involved in the transformation process from hair follicle stem cells to transit-amplifying cells and suggesting that β-catenin plays an important biological role in the induction of this differentiation process. 相似文献
19.
M. Raydan N. A. Shubin N. S. Nikolaenko M. I. Blinova G. G. Prokhorov G. P. Pinaev 《Cell and Tissue Biology》2011,5(3):294-299
Based on a previous study of the stability of a heterogenous population of keratinocytes against cold depending on their degree
of differentiation, we studied in vitro the stability of rat bone marrow stem cells (BMSCs) against cold before and after
their differentiation in the adipogenic or osteogenic direction. It was shown that, after the induction of differentiation,
BMSCs were least stable against the action of low temperatures than the undifferentiated cells. The obtained data can serve
as a basis for the further study of processes and mechanisms that affect the stability of BMSCs against cold depending on
their degree of differentiation. 相似文献
20.
《Cell cycle (Georgetown, Tex.)》2013,12(3):291-294
In vivo studies, transgenic and knock-out mice have demonstrated that p63 isoforms play pivotal roles in ectodermal and epidermal development but their respective function remains highly controversial. Since embryonic stem (ES) cells can be differentiated into many cell types, they represent an effective tool to recapitulate in vitro the main steps of embryonic development. We recently reported the efficient derivation of ectodermal and epidermal cells from murine ES cells and clarified the function of BMP-4 in the binary neuroectodermal choice by stimulating sox-1+ neural precursors to undergo specific apoptosis while inducing epidermal differentiation through ΔNp63 gene activation. ΔNp63 is not required for ectodermal fate but enhances ES-derived ectodermal cell proliferation and epidermal commitment. This unique cellular model should further provide a powerful tool for identifying the molecular mechanisms controlling normal skin development and in p63-ectodermal dysplasia human congenital pathologies. 相似文献