共查询到20条相似文献,搜索用时 46 毫秒
1.
《Bioscience, biotechnology, and biochemistry》2013,77(11):2188-2191
It has been reported that fructose force-feeding rapidly induced jejunal Slc2a5 gene expression in rodents. We demonstrate in this study that acetylation at lysine (K) 9 of histone H3 and acetylation at K5 and K16 of histone H4 were more enhanced in the promoter/enhancer to transcribed regions of the Slc2a5 gene in fructose force-fed mice than in glucose force-fed mice. However, fructose force-feeding did not induce acetylation at K14 of histone H3, or at K8 and K12 of histone H4 around the Slc2a5 gene. These results suggest that fructose force-feeding induced selective histone acetylation, particularly of H3 and H4, around the jejunal Slc2a5 gene in mice. 相似文献
2.
Yoshinaga Y Mochizuki K Goda T 《Biochemical and biophysical research communications》2012,419(4):605-611
We previously reported that fructose force-feeding rapidly induces jejunal Slc2a5 gene expression in rats. In this study, we conducted microarray analyses using total RNA to identify genes upregulated in rat jejunum by fructose force-feeding. Rats were force-fed fructose, glucose or distilled water for 6h. Genes such as Slc2a5, Cdkn1c, Cabp2, Ranbp3, Vwce and Gcgr were induced by force-feeding with fructose compared with glucose or distilled water. Chromatin immunoprecipitation assays revealed that trimethylation of histone H3K4, and acetylation of histones H3 and H4, on the transcribed region of these fructose-inducible genes were enhanced by force-feeding of fructose, but not glucose or distilled water. These results suggest that the induction of genes in the rat jejunum by fructose force-feeding is coordinately regulated by histone modifications, particularly trimethylation of histone H3K4. 相似文献
3.
4.
5.
Hiroyuki Yamauchi Kazue Honma Kazuki Mochizuki 《Bioscience, biotechnology, and biochemistry》2018,82(7):1176-1179
Jejunal sodium/glucose co-transporter (Sglt1) displays circadian expression. The jejunum was collected every 4 h from mice, and we examined histone acetylation and binding of bromodomain-containing protein-4 (BRD4) around of the gene. Histone acetylation increased in the transcribed region of Sglt1 prior to induction of the gene. Furthermore, the binding of mRNA elongation factor around the gene showed circadian rhythm. 相似文献
6.
7.
8.
9.
Smith JA Kohn TA Chetty AK Ojuka EO 《American journal of physiology. Endocrinology and metabolism》2008,295(3):E698-E704
The role of CaMK II in regulating GLUT4 expression in response to intermittent exercise was investigated. Wistar rats completed 5 x 17-min bouts of swimming after receiving 5 mg/kg KN93 (a CaMK II inhibitor), KN92 (an analog of KN93 that does not inhibit CaMK II), or an equivalent volume of vehicle. Triceps muscles that were harvested at 0, 6, or 18 h postexercise were assayed for 1) CaMK II phosphorylation by Western blot, 2) acetylation of histone H3 at the Glut4 MEF2 site by chromatin immunoprecipitation (ChIP) assay, 3) bound MEF2A at the Glut4 MEF2 cis-element by ChIP, and 4) GLUT4 expression by RT-PCR and Western blot. Compared with controls, exercise caused a twofold increase in CaMK II phosphorylation. Immunohistochemical stains indicated increased CaMK II phosphorylation in nuclear and perinuclear regions of the muscle fiber. Acetylation of histone H3 in the region surrounding the MEF2 binding site on the Glut4 gene and the amount of MEF2A that bind to the site increased approximately twofold postexercise. GLUT4 mRNA and protein increased approximately 2.2- and 1.8-fold, respectively, after exercise. The exercise-induced increases in CaMK II phosphorylation, histone H3 acetylation, MEF2A binding, and GLUT4 expression were attenuated or abolished when KN93 was administered to rats prior to exercise. KN92 did not affect the increases in pCaMK II and GLUT4. These data support the hypothesis that CaMK II activation by exercise increases GLUT4 expression via increased accessibility of MEF2A to its cis-element on the gene. 相似文献
10.
Hongye Hu Enjiu Chen Yongji Li Xiaochun Zhu Ting Zhang Xiaofang Zhu 《Biological trace element research》2018,184(2):391-397
Arsenic trioxide (As2O3; ATO), a traditional Chinese medicine, is used to treat patients with acute promye-locytic leukemia, while its application for treatment of systemic lupus erythematosus (SLE) is still under evaluation. The high expression of INF-gamma (INF-γ) is a primary pathogenic factor in SLE. It is found that ATO can reduce INF-γ expression levels in lupus-prone mice, whereas it is not clear whether ATO has the same effect on SLE patients. Therefore, this study was to investigate the underlying mechanism of the effects of ATO on the expression of INF-γ in splenocytes of MRL/lpr mice and PBMCs of human lupus. The mRNA and protein expression levels of INF-γ were assessed by real-time RT-PCR and ELISA, respectively. The histone acetylation status of the INF-γ promoter and the binding of RNA polymerase II (RNA Pol II) to the INF-γ promoter were detected using a chromatin immunoprecipitation (ChIP) technique. The mRNA and protein expression levels of INF-γ decreased in both splenocytes of MRL/lpr mice and PBMCs of SLE patients with ATO treatment, which were accompanied by reduced histone H4 and H3 acetylation in INF-γ promoter and decreased combination of RNA Pol II to the INF-γ promoter. Therefore, ATO may reduce the expression level of the INF-γ by altering the levels of INF-γ promoter acetylation and the combination of RNA Pol II to the INF-γ promoter in splenocytes of MRL/lpr mice and PBMCs of SLE patients. 相似文献
11.
12.
13.
14.
15.
16.
17.
18.
19.
Sharon Barone Stacey L. Fussell Anurag Kumar Singh Fred Lucas Jie Xu Charles Kim Xudong Wu Yiling Yu Hassane Amlal Ursula Seidler Jian Zuo Manoocher Soleimani 《The Journal of biological chemistry》2009,284(8):5056-5066
The identity of the transporter responsible for fructose absorption in the
intestine in vivo and its potential role in fructose-induced
hypertension remain speculative. Here we demonstrate that Glut5 (Slc2a5)
deletion reduced fructose absorption by ∼75% in the jejunum and decreased
the concentration of serum fructose by ∼90% relative to wild-type mice on
increased dietary fructose. When fed a control (60% starch) diet,
Glut5-/- mice had normal blood pressure and displayed normal weight
gain. However, whereas Glut5+/+ mice showed enhanced salt
absorption in their jejuna in response to luminal fructose and developed
systemic hypertension when fed a high fructose (60% fructose) diet for 14
weeks, Glut5-/- mice did not display fructose-stimulated salt
absorption in their jejuna, and they experienced a significant impairment of
nutrient absorption in their intestine with accompanying hypotension as early
as 3–5 days after the start of a high fructose diet. Examination of the
intestinal tract of Glut5-/- mice fed a high fructose diet revealed
massive dilatation of the caecum and colon, consistent with severe
malabsorption, along with a unique adaptive up-regulation of ion transporters.
In contrast to the malabsorption of fructose, Glut5-/- mice did not
exhibit an absorption defect when fed a high glucose (60% glucose) diet. We
conclude that Glut5 is essential for the absorption of fructose in the
intestine and plays a fundamental role in the generation of fructose-induced
hypertension. Deletion of Glut5 results in a serious nutrient-absorptive
defect and volume depletion only when the animals are fed a high fructose diet
and is associated with compensatory adaptive up-regulation of ion-absorbing
transporters in the colon.Fructose is a monosaccharide and is one of the three most important blood
sugars along with glucose and galactose
(1–3).
It plays an essential role in vital metabolic functions in the body, including
glycolysis and gluconeogenesis
(4–6).
Fructose is predominantly metabolized in the liver. A high flux of fructose to
the liver perturbs glucose metabolism and leads to a significantly enhanced
rate of triglyceride synthesis. In addition, fructose can be metabolized in
the liver to uric acid, a potent antioxidant
(7,
8).The classic model of sugar absorption indicates that sodium glucose
cotransporter 1
(Sglt1)3 and Glut5
absorb glucose and fructose, respectively, from intestinal lumen to cytosol,
and Glut2 transports both glucose and fructose from the cytosol to the blood
(9–19).
Glut2 has high affinity for glucose and a moderate affinity for fructose,
whereas Glut5 predominantly transports fructose with very low affinity for
glucose
(9–19;
reviews in Refs. 14,
17–19).
The expression of Glut5 or Glut2 in the small intestine increases in rats or
mice fed a diet high in fructose or perfused with increased fructose
concentration
(11–14,
18,
19).Glut2 is predominantly found on the basolateral membrane and in the
cytoplasm of enterocytes at basal state but is thought to be recruited to the
apical membrane in the presence of increased glucose or fructose in the
intestinal lumen (11,
19). Given the fact that both
Glut5 and Glut2 can transport fructose in vitro and given the ability
of Glut2 to traffic to the apical membrane, the contribution of Glut5 to the
absorption of fructose in vivo and systemic fructose homeostasis
remains speculative.The marked increase in dietary fructose consumption in the form of high
fructose corn syrup, a common sweetener used in the food industry, table
sugar, and fruits correlates with the increased incidence of metabolic
syndrome, which is reaching an epidemic proportion in developed countries and
is a major contributor to premature morbidity and mortality in our society
(20–22).
Increased dietary fructose intake recapitulates many aspects of metabolic
syndrome, including dyslipidemia, insulin resistance, and hypertension in rat
and mouse
(23–26).
Recent studies demonstrate that fructose-induced hypertension is initiated by
increased absorption of salt and fructose in the intestine
(27); however, the one or more
molecules (Glut2, Glut5, Glut7, or Sglt1) that are responsible for the
absorption of fructose in the intestine remain speculative. Further, although
Glut7, Glut5, and Glut2 can transport fructose in vitro, the role of
Glut5 in in vivo fructose absorption remains unknown. To ascertain
the role of Glut5 in fructose absorption in the intestine in vivo and
fructose-induced hypertension, mice lacking the Glut5 gene
(Glut5-/-) were placed on either high fructose or normal diet and
compared with their wild-type littermates (Glut5+/+). 相似文献