大气臭氧浓度升高对植物及其昆虫的影响

臭氧是最具危害性的空气污染物之一,从19世纪中叶开始,对流层中的臭氧水平增加了35％,在今后50—100年内还将继续升高。臭氧浓度升高对植物生理基本功能、植物体内信号分子以及挥发物都具有不同程度的影响,并严重影响作物的产量。臭氧也通过改变植物的原生代谢和次生代谢发生数量而影响植食性昆虫的取食偏嗜性、行为、生长和发育,进而影响天敌昆虫的适合度。臭氧还通过改变化学信息物质而影响昆虫的行为。根据国内外研究进展,介绍了大气臭氧浓度升高对植物、昆虫的影响,并讨论了目前存在的问题和研究前景。     

大气CO2浓度增加对昆虫的影响

大气CO2浓度增加已经受到国内外的极大关注.CO2浓度升高不但影响植物的生长发育,而且还改变植物体内的化学成分的组成与含量,从而间接地影响到植食性昆虫,并进而通过食物链影响到以之为食的天敌.根据国内外研究进展,结合多年的研究,系统介绍了CO2浓度变化对植物-昆虫系统影响的研究方法,论述了CO2浓度变化对植食性昆虫、天敌的作用规律及作用机理,探讨了CO2浓度变化对植物-植食性昆虫系统影响的特征,分析了未来研究发展的趋势及其存在的问题.     

载体植物在温室作物害虫生物防治中的应用

增补式生物防治是利用天敌昆虫或捕食螨防治害虫最常用的方法,释放的天敌昆虫或捕食螨能否成功建立稳定种群,是决定其持久高效控害的首要关键因素。然而在生产应用中,经常因释放的天敌昆虫或捕食螨不能成功定殖而无法达到理想的防治效果。载体植物系统是天敌饲养和释放的新方法,结合了增补式和保护式生物防治的优点,既能实现天敌昆虫或捕食螨的大量饲养,又能为释放的天敌昆虫或捕食螨提供替代食物和栖息场所,促进其建立稳定的种群,对实现天敌昆虫或捕食螨高效持久控害具有重要的意义。本文系统介绍了载体植物系统的构建、国内外研究现状,指出了现存的问题,并给出相应的建议。     

长白山阔叶红松林植物群落与昆虫群落的相互关系研究

通过季节性的观察,系统研究了长白山植物群落、昆虫群落多样性及它们之间关系。结果表明,植物群落物种丰富度指数与植食性昆虫丰富度指数呈负相关,而与捕食性天敌昆虫丰富度指数呈正相关,与寄生性天敌的丰富度指数呈负相关;昆虫群落的均匀度与植物群落的均匀度相关显著。植物群落的多样性指数与捕食性、寄生性天敌的多样性指数呈正相关。而与植食性昆虫的多样性指数呈负相关,这表明植物群落的多样性影响昆虫群落的多样性。     

蜜粉源植物对天敌昆虫的作用及其在生物防治中的应用

寄生性和捕食性天敌昆虫成虫普遍存在通过取食蜜粉源植物补充营养的行为,这可不同程度地促进天敌昆虫性成熟、延长其寿命、提高其生殖力或寄生率,以及搜寻寄主效率和子代雌性比率,从而显著提高天敌昆虫在生物防治中的控害能力和效果。蜜粉源植物花的结构及植物对天敌昆虫产生的嗅觉、视觉信号和花蜜花粉对天敌昆虫产生的味觉信号又显著影响天敌昆虫选择蜜粉源植物的行为和结果。但是,蜜粉源植物也可成为害虫的补充营养植物,从而提高害虫的为害能力。因此,需深入研究不同蜜粉源植物对天敌昆虫及害虫的作用,趋利避害,才可能应用蜜粉源植物成功调控天敌与害虫的益害比,实现害虫的可持续控制。     

《天敌昆虫应用原理和方法》

由湖南农业大学、中南林学院编著的《天敌昆虫应用原理和方法》一书于 2 0 0 3年 1 0月由湖南科技出版社出版 ,共41万字。包括天敌昆虫类群、寄生与寄生现象的进化、保护利用、天敌昆虫和昆虫地理学、杂草和天敌昆虫、天敌昆虫在农业生态系统中的地位及其特点、天敌种群生态学、群落生态学、天敌昆虫滞育和迁飞、性信息素和利它素、化学防治和微生物杀虫剂对天敌昆虫的影响、抗虫育种与天敌昆虫的关系、转基因作物抗虫研究与天敌昆虫、水生捕食性天敌昆虫和水质监测、天敌昆虫和植物检疫、引进天敌昆虫的风险评价、天敌昆虫鉴定、天敌昆虫大…     

稻田中性昆虫对群落食物网的调控作用

本文把稻田既非害虫也非天敌的一类昆虫称为中性昆虫,应用选择性控制法（扑虱灵和杀蚊幼剂＾－ＢＳ）、挤压法、脉冲试验法及通径分析研究了稻田中性昆虫的调控作用;中性昆虫与天敌、害虫的直接和间接作用规律,结果证明中性昆早虫数量增加,中必一昆虫与害虫呈显著的负相关,与天敌呈显著的正相关,中性昆虫对害虫的调控主要是通过影响捕食性天敌而实现的。     

刺吸式口器昆虫吸食量测定

<正> 就咀嚼式口器昆虫取食研究,由于其取食行为较为直观,经常采用称重法,直接测定昆虫某一龄期或整个一生的取食量。但刺吸式口器的昆虫,其取食对象是寄主的汁液,而且取食行为较为复杂,加上对昆虫人工饲料筛选的难度,尽管许多学者采用了许多方法,但还不能精确测定其吸食量。本文就刺吸式口器昆虫吸食量的研究方法作一概述。     

大气二氧化碳浓度升高对植物-昆虫相互关系的影响

综述了CO2浓度升高对植物与昆虫相互关系影响的研究结果。大量研究表明,高浓度CO2对植物生理生化活动有显著的影响,植物营养物质的变化对植食性昆虫亦产生不同程度的影响,高浓度CO2条件对咀嚼式口器昆虫的取食、生长发育和生殖有不同程度的不良影响,昆虫为了获得足够的氮素营养而增加取食强度和时间,从而更易于受到天敌的攻击,这些昆虫的生长率、繁殖和生存率有下降的趋势;而对刺吸韧皮部汁液的昆虫来说,多引起种群数量增加或无显著影响。并对研究中存在的问题进行了分析,提出了今后研究的方向。     

虫粪中信息化学物质的研究进展

排便是昆虫的一个重要生理过程,排便行为不仅可以清除食物残渣,而且可以释放某些化学信息,进而影响昆虫同种或异种之间的相互作用.昆虫粪便中的信息化学物质不仅影响同种昆虫的聚集、求偶行为,而且可以影响同种和异种昆虫的产卵行为.此外,虫粪还能为其天敌昆虫的寄主定位和识别提供信息,并能通过诱导植物的防御反应抑制植食性昆虫的取食行为.本文根据国内外的相关研究,对虫粪中存在的信息化学物质进行了分类总结,并介绍了虫粪信息化合物在害虫防治中的应用,还讨论了目前存在的问题和研究前景.     

Sub-Parts-Per-Billion Nitrate Method: Use of an N(2)O-Producing Denitrifier to Convert NO(3) or NO(3) to N(2)O

A more sensitive analytical method for NO(3) was developed based on the conversion of NO(3) to N(2)O by a denitrifier that could not reduce N(2)O further. The improved detectability resulted from the high sensitivity of the Ni electron capture gas chromatographic detector for N(2)O and the purification of the nitrogen afforded by the transformation of the N to a gaseous product with a low atmospheric background. The selected denitrifier quantitatively converted NO(3) to N(2)O within 10 min. The optimum measurement range was from 0.5 to 50 ppb (50 mug/liter) of NO(3) N, and the detection limit was 0.2 ppb of N. The values measured by the denitrifier method compared well with those measured by the high-pressure liquid chromatographic UV method above 2 ppb of N, which is the detection limit of the latter method. It should be possible to analyze all types of samples for nitrate, except those with inhibiting substances, by this method. To illustrate the use of the denitrifier method, NO(3) concentrations of <2 ppb of NO(3) N were measured in distilled and deionized purified water samples and in anaerobic lake water samples, but were not detected at the surface of the sediment. The denitrifier method was also used to measure the atom% of N in NO(3). This method avoids the incomplete reduction and contamination of the NO(3) -N by the NH(4) and N(2) pools which can occur by the conventional method of NO(3) analysis. N(2)O-producing denitrifier strains were also used to measure the apparent K(m) values for NO(3) use by these organisms. Analysis of N(2)O production by use of a progress curve yielded K(m) values of 1.7 and 1.8 muM NO(3) for the two denitrifier strains studied.     

To what extent do current and projected increases in surface ozone affect photosynthesis and stomatal conductance of trees? A meta‐analytic review of the last 3 decades of experiments

The surface concentration of ozone ([O(3)]) has risen from less than 10 ppb prior to the industrial revolution to a day-time mean concentration of approximately 40 ppb over much of the northern temperate zone. If current global emission trends continue, surface [O(3)] is projected to rise a further 50% over this century, with larger increases in many locations including Northern Hemisphere forests. This review uses statistical meta-analysis to determine mean effects, and their confidence limits, of both the current and projected elevations of [O(3)] on light-saturated photosynthetic CO(2) uptake (A(sat)) and stomatal conductance (g(s)) in trees. In total, 348 measurements of A(sat) from 61 studies and 266 measures of g(s) from 55 studies were reviewed. Results suggested that the elevation of [O(3)] that has occurred since the industrial revolution is depressing A(sat) and g(s) by 11% (CI 9-13%) and 13% (CI 11-15%), respectively, where CI is the 95% confidence interval. In contrast to angiosperms, gymnosperms were not significantly affected. Both drought and elevated [CO(2)] significantly decreased the effect of ambient [O(3)]. Younger trees (<4 years) were affected less than older trees. Elevation of [O(3)] above current levels caused progressively larger losses of A(sat) and g(s), including gymnosperms. Results are consistent with the expectation that damage to photosynthesis depends on the cumulative uptake of ozone (O(3)) into the leaf. Thus, factors that lower g(s) lessen damage. Where both g(s) and [O(3)] were recorded, an overall decline in A(sat) of 0.21% per mmol m(-2) of estimated cumulative O(3) uptake was calculated. These findings suggest that rising [O(3)], an often overlooked aspect of global atmospheric change, is progressively depressing the ability of temperate and boreal forests to assimilate carbon and transfer water vapour to the atmosphere, with significant potential effects on terrestrial carbon sinks and regional hydrologies.     

Air pollution distribution patterns in the San Bernardino Mountains of southern California: a 40-year perspective

Since the mid-1950s, native pines in the San Bernardino Mountains (SBM) in southern California have shown symptoms of decline. Initial studies in 1963 showed that ozone (O3) generated in the upwind Los Angeles Basin was responsible for the injury and decline of sensitive trees. Ambient O3 decreased significantly by the mid-1990s, resulting in decreased O3 injury and improved tree growth. Increased growth of trees may also be attributed to elevated atmospheric nitrogen (N) deposition. Since most of the N deposition to mixed conifer forest stands in the SBM results from dry deposition of nitric acid vapor (HNO3) and ammonia (NH3), characterization of spatial and temporal distribution of these two pollutants has become essential. Although maximum daytime O3 concentrations over last 40 years have significantly decreased (approximately 3-fold), seasonal means have been reduced much less (approximately 1.5-fold), with 2-week long means occasionally exceeding 100 ppb in the western part of the range. In the same area, significantly elevated concentrations of HNO3 and NH3, up to 17.5 and 18.5 microg/m3 as 2-week averages, respectively, have been determined. Elevated levels of O3 and increased N deposition together with long-term drought predispose the SBM forests to massive bark beetle attacks making them susceptible to catastrophic fires.     

Root and shoot gas exchange respond additively to moderate ozone and methyl jasmonate without induction of ethylene: ethylene is induced at higher O3 concentrations

The available literature is conflicting on the potential protection of plants against ozone (O(3)) injury by exogenous jasmonates, including methyl jasmonate (MeJA). Protective antagonistic interactions of O(3) and MeJA have been observed in some systems and purely additive effects in others. Here it is shown that chronic exposure to low to moderate O(3) concentrations (4-114 ppb; 12 h mean) and to MeJA induced additive reductions in carbon assimilation (A (n)) and root respiration (R (r)), and in calculated whole plant carbon balance. Neither this chronic O(3) regime nor MeJA induced emission of ethylene (ET) from the youngest fully expanded leaves. ET emission was induced by acute 3 h pulse exposure to much higher O(3) concentrations (685 ppb). ET emission was further enhanced in plants treated with MeJA. Responses of growth, allocation, photosynthesis, and respiration to moderate O(3) concentrations and to MeJA appear to be independent and additive, and not associated with emission of ET. These results suggest that responses of Pima cotton to environmentally relevant O(3) are not mediated by signalling pathways associated with ET and MeJA, though these pathways are inducible in this species and exhibit a synergistic O(3)×MeJA interaction at very high O(3) concentrations.     

Chronic vs. short-term acute O3 exposure effects on nocturnal transpiration in two Californian oaks

We tested the effect of daytime chronic moderate ozone (O3) exposure, short-term acute exposure, and both chronic and acute O3 exposure combined on nocturnal transpiration in California black oak and blue oak seedlings. Chronic O3 exposure (70 ppb for 8 h/day) was implemented in open-top chambers for either 1 month (California black oak) or 2 months (blue oak). Acute O3 exposure (approximately 1 h in duration during the day, 120-220 ppb) was implemented in a novel gas exchange system that supplied and maintained known O3 concentrations to a leaf cuvette. When exposed to chronic daytime O3 exposure, both oaks exhibited increased nocturnal transpiration (without concurrent O3 exposure) relative to unexposed control leaves (1.8x and 1.6x, black and blue oak, respectively). Short-term acute and chronic O3 exposure did not further increase nocturnal transpiration in either species. In blue oak previously unexposed to O3, short-term acute O3 exposure significantly enhanced nocturnal transpiration (2.0x) relative to leaves unexposed to O3. California black oak was unresponsive to (only) short-term acute O3 exposure. Daytime chronic and/or acute O3 exposures can increase foliar water loss at night in deciduous oak seedlings.     

Lethal and sublethal effects of imidacloprid on Osmia lignaria and clothianidin on Megachile rotundata (Hymenoptera: Megachilidae)

We examined lethal and sublethal effects of imidacloprid on Osmia lignaria (Cresson) and clothianidin on Megachile rotundata (F.) (Hymenoptera: Megachilidae). We also made progress toward developing reliable methodology for testing pesticides on wild bees for use in pesticide registration by using field and laboratory experiments. Bee larvae were exposed to control, low (3 or 6 ppb), intermediate (30 ppb), or high (300 ppb) doses of either imidacloprid or clothianidin in pollen. Field experiments on both bee species involved injecting the pollen provisions with the corresponding pesticide. Only O. lignaria was used for the laboratory experiments, which entailed both injecting the bee's own pollen provisions and replacing the pollen provision with a preblended pollen mixture containing imidacloprid. Larval development, emergence, weight, and mortality were monitored and analyzed. There were no lethal effects found for either imidacloprid or clothianidin on O. lignaria and M. rotundata. Minor sublethal effects were detected on larval development for O. lignaria, with greater developmental time at the intermediate (30 ppb) and high doses (300 ppb) of imidacloprid. No similar sublethal effects were found with clothianidin on M. rotundata. We were successful in creating methodology for pesticide testing on O. lignaria and M. rotundata; however, these methods can be improved upon to create a more robust test. We also identified several parameters and developmental stages for observing sublethal effects. The detection of sublethal effects demonstrates the importance of testing new pesticides on wild pollinators before registration.     

A test of the oxidative damage hypothesis for discontinuous gas exchange in the locust Locusta migratoria

The discontinuous gas exchange cycle (DGC) is a breathing pattern displayed by many insects, characterized by periodic breath-holding and intermittently low tracheal O(2) levels. It has been hypothesized that the adaptive value of DGCs is to reduce oxidative damage, with low tracheal O(2) partial pressures (PO(2) ≈ 2-5 kPa) occurring to reduce the production of oxygen free radicals. If this is so, insects displaying DGCs should continue to actively defend a low tracheal PO(2) even when breathing higher than atmospheric levels of oxygen (hyperoxia). This behaviour has been observed in moth pupae exposed to ambient PO(2) up to 50 kPa. To test this observation in adult insects, we implanted fibre-optic oxygen optodes within the tracheal systems of adult migratory locusts Locusta migratoria exposed to normoxia, hypoxia and hyperoxia. In normoxic and hypoxic atmospheres, the minimum tracheal PO(2) that occurred during DGCs varied between 3.4 and 1.2 kPa. In hyperoxia up to 40.5 kPa, the minimum tracheal PO(2) achieved during a DGC exceeded 30 kPa, increasing with ambient levels. These results are consistent with a respiratory control mechanism that functions to satisfy O(2) requirements by maintaining PO(2) above a critical level, not defend against high levels of O(2).     

Supplemental ultraviolet-B and ozone: impact on antioxidants, proteome and genome of linseed (Linum usitatissimum L. cv. Padmini)

The present investigation used Linum usitatissimum L. cv. Padmini (linseed), under field conditions in open-top chambers, to evaluate the interactive effects of supplemental ultraviolet-B (sUV-B; ambient +7.2 kJ · m(-2) · d(-1)) and ozone (O(3); ambient +10 ppb). Treatment of plants with sUV-B and O(3) , individually or in combination, caused several changes in enzymatic and non-enzymatic components of the antioxidant defence system. Photo-oxidative damage caused by sUV-B and O(3) , included lipid peroxidation, changed protein profiles and caused DNA strand breakage. One-dimensional gel electrophoresis revealed that proteins of 222.24 and 50.5 kDa are specific and appear after sUV-B and O(3) exposure, and could be used as indicator proteins. Effects of sUV-B and O(3) given separately are more detrimental as compared to combined treatment. Mutational and structural alterations in linseed DNA after these stresses were also examined using RAPD with ten different primers. The study concluded that both stresses, i.e. sUV-B and O(3) , are phytotoxic, causing significant changes in metabolites, antioxidants, the leaf proteome and the genome of linseed, but their interactive effect was always less than additive.     

Rising ozone concentrations decrease soybean evapotranspiration and water use efficiency whilst increasing canopy temperature

? Here, we investigated the effects of increasing concentrations of ozone ([O(3)]) on soybean canopy-scale fluxes of heat and water vapor, as well as water use efficiency (WUE), at the Soybean Free Air Concentration Enrichment (SoyFACE) facility. ? Micrometeorological measurements were made to determine the net radiation (R(n)), sensible heat flux (H), soil heat flux (G(0)) and latent heat flux (λET) of a commercial soybean (Glycine max) cultivar (Pioneer 93B15), exposed to a gradient of eight daytime average ozone concentrations ranging from approximately current (c. 40 ppb) to three times current (c. 120 ppb) levels. ? As [O(3)] increased, soybean canopy fluxes of λET decreased and H increased, whereas R(n) and G(0) were not altered significantly. Exposure to increased [O(3)] also resulted in warmer canopies, especially during the day. The lower λET decreased season total evapotranspiration (ET) by c. 26%. The [O(3)]-induced relative decline in ET was half that of the relative decline in seed yield, driving a 50% reduction in seasonal WUE. ? These results suggest that rising [O(3)] will alter the canopy energy fluxes that drive regional climate and hydrology, and have a negative impact on productivity and WUE, key ecosystem services.