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用超滤、硫酸铵二段盐析法取代等电点沉淀法后,精制破伤风类毒素(精破类)的纯度由807Lf/mgPN提高到1883Lf/mgPN,纯度提高一倍以上。使用双胨培养基取代酪素培养基后,产毒水平由47Lf/ml提高到88Lf/ml(t=6.46,p<0.001);用新法精制后,精破类纯度分别为1949Lf/mgPN及1785Lf/mgPN(t=0.334,p>0.05),引用双胨培养基后可提高产毒水平,但不影响精破类的纯度。 相似文献
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Antigenic and immunogenic studies on purified tetanus toxoid 总被引:1,自引:0,他引:1
P A Nielsen F B Ablondi M V Querry C Gussoni M S Cooper 《Journal of immunology (Baltimore, Md. : 1950)》1967,98(6):1248-1256
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A sulfhydryl-dependent protease (SHP) was purified from a culture of Clostridium botulinum type F. The enzyme can activate type E progenitor toxin completely but type B progenitor toxin only partially. This may suggest that SHP by itself could completely activate the toxin of proteolytic C. botulinum types A and F in culture. The toxicity of type E progenitor toxin potentiated by the treatment with SHP persisted, whereas that of derivative toxin decreased rapidly by further incubation with SHP. This may indicate that only the progenitor toxin, the complex of the toxic and nontoxic components, activated by SHP withstands the subsequent exposure to the enzyme in cultures of proteolytic C. botulinum. 相似文献
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