共查询到20条相似文献,搜索用时 31 毫秒
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Y. Liang Y. Wang L. Ma Z. Zhong X. Yang X. Tao X. Chen Z. He Y. Yang K. Zeng R. Kang J. Gong S. Ying Y. Lei J. Pang X. Lv Y. Gu 《Animal genetics》2019,50(5):439-448
Elucidation of the pig microRNAome is essential for interpreting functional elements of the genome and understanding the genetic architecture of complex traits. Here, we extracted small RNAs from skeletal muscle and adipose tissue, and we compared their expression levels between one Western breed (Yorkshire) and seven indigenous Chinese breeds. We detected the expression of 172 known porcine microRNAs (miRNAs) and 181 novel miRNAs. Differential expression analysis found 92 and 12 differentially expressed miRNAs in adipose and muscle tissue respectively. We found that different Chinese breeds shared common directional miRNA expression changes compared to Yorkshire pigs. Some miRNAs differentially expressed across multiple Chinese breeds, including ssc‐miR‐129‐5p, ssc‐miR‐30 and ssc‐miR‐150, are involved in adipose tissue function. Functional enrichment analysis revealed that the target genes of the differentially expressed miRNAs are associated mainly with signaling pathways rather than metabolic and biosynthetic processes. The miRNA–target gene and miRNA–phenotypic traits networks identified many hub miRNAs that regulate a large number of target genes or phenotypic traits. Specifically, we found that intramuscular fat content is regulated by the greatest number of miRNAs in muscle tissue. This study provides valuable new candidate miRNAs that will aid in the improvement of meat quality and production. 相似文献
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Xihui Sheng Xuemei Song Yan Yu Lili Niu Shangang Li Hongbin Li Caihong Wei Tao Liu Li Zhang Lixin Du 《Molecular biology reports》2011,38(5):3161-3171
Ovis aries is one of the most important agricultural livestock for meat production, and also is an ideal model organism for biological
and comparative genomics studies. Many miRNAs have been reported for their important roles in developmental processes in various
animals, but there is limited information about O. aries miRNAs. In this study, combining a computational method based on expressed sequence tag (EST) analysis with experimental
identification based on small RNA cDNA library, we identified 31 miRNAs belong to 24 families in sheep, 2 of which were novel
miRNAs which had never been previously identified in any species. Especially, we cloned 12 miRNAs from the sheep skeletal
muscle, which were good candidate miRNAs to be studied about the miRNA-dependant regulated process of muscle development,
and we identified four pairs of miRNA/miRNA* and one pair of miRNA-3p/miRNA-5p from sheep EST sequences. Expression analysis
indicated that some miRNAs were expressed in a specific tissue, and the pair of miRNA-3p/miRNA-5p and one pair of miRNA/miRNA*
had a similar relative expression pattern in some tissues, respectively. Further, we predicted 120 potential target genes
of 31 oar-miRNAs on the 3′UTR of O. aries genes. Gene ontology analysis showed that most of these genes took part in the cellular process and metabolic process. Our
results enriched the O. aries miRNA database and provided useful information for investigating biological functions of miRNAs and miRNA* in sheep. 相似文献
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MicroRNA maturation: stepwise processing and subcellular localization 总被引:71,自引:0,他引:71
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The DNA damage response involves a complex network of processes that detect and repair DNA damage. Here we show that miRNA biogenesis is globally induced upon DNA damage in an ATM-dependent manner. About one-fourth of miRNAs are significantly upregulated after DNA damage, while loss of ATM abolishes their induction. KH-type splicing regulatory protein (KSRP) is a key player that translates DNA damage signaling to miRNA biogenesis. The ATM kinase directly binds to and phosphorylates KSRP, leading to enhanced interaction between KSRP and pri-miRNAs and increased KSRP activity in miRNA processing. Mutations of the ATM phosphorylation sites of KSRP impaired its activity in regulating miRNAs. These findings reveal a mechanism by which DNA damage signaling is linked to miRNA biogenesis. 相似文献
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Kawahara Y Megraw M Kreider E Iizasa H Valente L Hatzigeorgiou AG Nishikura K 《Nucleic acids research》2008,36(16):5270-5280