Action de l'ecdystérone sur l'apolysis et l'ecdysis de divers crustacés isopodes

Ecdysterone acts differently according to the period of the moulting cycle when it is applied. In period C it induces apolysis, while at the beginning of apolysis it delays ecdysis. Experiments show that the exoskeleton cannot be rejected in the presence of ecdysterone. The possibility of stopping exuviation or ecdysis with ecdysterone seems to prove that this phenomenon is controlled by a particular factor called the factor of exuviation or the ecdysis factor. Ecdysterone would control only the release of this specific factor but would not prevent its action.     

Purification de l'alcool deshydrogénase de tomate par chromatographie d'affinité

Tomato alcohol dehydrogenase has been purified 99-fold by affinity chromatography on Blue Sepharose CL-6B with 37% yield. The enzyme so obtained is homogenous in polyacrylamide gel electrophoresis. By adding 20% glycerol to the extraction and purification buffers, an enzyme is obtained which is stable for several months at 4°. The molecular weight values determined by gel filtration (Sephadex G 200) and polyacrylamide gradient gel electrophoresis on one hand and by polyacrylamide gel electrophoresis in sodium dodecyl sulfate on the other, show that the enzyme exists in dimeric form.     

Relation métabolique entre l'arginine et l'acide γ-aminobutyrique dans le fruit de Castanea sativa: étude préliminaire de l'arginase

Analysis of the free amino acid pool in the chestnut fruit (Castanea sativa) shows that it is high in γ-aminobutyric acid. A metabolic connection between arginine and γ-aminobutyric acid is evident. Three enzymatic activities are involved: L-arginine ureohydrolase, L-ornithine 2-oxo-acid aminotransferase and L-glutamate carboxylyase.     

Action de l'antimycine a sur l'activité succinoxydase des mitochondries du tubercule de Pomme de Terre,et comportement des cytochromes de type b

A kinetic study of the effect of antimycin A on succinate oxidase from plant mitochondria produced sigmoidal curves for the reduction of cytochromes b₅₆₀ and b₅₈₅ and for the inhibition of succinate oxidase. In the stationary state the interaction of the various components of the respiratory chain (flavins, ubiquinone, cytochromes…) occurs in a sequential mode which allows the application of simple equations utilizing rate constants and cytochrome concentrations. In these equations, it is assumed that there exists an excess of ubiquinone relative to other components of the respiratory chain as suggested by Kröger & Klingenberg (1970) and that the reoxidation of b cytochromes is fast. The inhibition by antimycin A, characterized by non-linear inhibition curves for succin-oxidase and inhibitor fixation in complex III on a component other than cytochrome c₁, is interpreted in terms of this model. This hypothesis presupposes the existence of an F factor between cytochrome b₅₆₀ and cytochrome c₁ as suggested by other authors. Utilizing these equations, theoretical curves for the inhibition of the reduction of cytochrome b₅₆₀ have been constructed and the results agree with the experimental data. The kinetic behavior of cytochrome b₅₆₆ during the induction of anaerobiosis suggests that it is not directly involved in the electron transfer chain but rather is either in thermodynamic equilibrium with cytochrome b₅₆₀ or in a shunt between cytochrome b₅₆₀ and factor F. From the experimental data, an equation is derived for the inhibition of the reduction of cytochrome b₅₆₆ by antimycin A. The actual effects of ATP and mClCCP on succinoxidase agree well with those predicted by the model.     

Stabilité de l'espèce et évolution: La théorie de l'équilibre intermittent («Punctuated equilibrium)

Paleontologists' traditional conceptions on evolution do not seem to be in perfect accord with these of biologists. The gradual phyletism is compatible neither with E. Mayr's model of the Founder, nor with the actual stability of most of the paleontological species. The punctuated equilibrium model proposed by N. Eldredge & S.J. Gould offers a solution which integrates both paleontological observations and the data of modern biology.     

Rôle des organes pulsatiles thoraciques et du cœur pendant l'émergence et l'expansion des ailes des lépidoptères

Electrograms of the thoracic pulsatile organs and of the heart during emergence and expansion of the wings have been made. The mesotergal and metatergal organs function in a similar way: active periods are interrupted by short periods of rest, but the two organs contract with different but apparently independent rhythms. During emergence and expansion, the rate of contraction of the pulsatile organs increases twofold, and the resting periods disappear during the entire period of expansion of the wings. When the wings are removed or cut short, the activity of the pulsatile organs is inhibited.     

Utilisation de lois de la mécanique pour l'Estimationde la vitesse de locomotion des Vertébrés tétrapodes du passé

Two computation methods are explained; theirobject is the estimation of the velocity of animals which are known by their footprints, and in the case of the first method, by their skeletons as well.The first method is based on the compound pendulum theory, because during the slow walking gait, the motion of the leg is similar to the oscillation of a pendulum, for the computation of the velocity (v), are considered: moment of inertia (I), radius of gyration (P) and period (T), time in seconds to cover one stride (E) in the case the maximum angle of divarication (δ) of the leg with the vertical is ≤20°. A comparison with the formula of Alexander (1976) is discussed.The second method concerns saltator animals. It is based on the fondamental laws of dynamics. With the length of the jump (E) it is possible to estimate the velocity of the trackmaker (v) and the height of the jump (K). For the vertebrates the angle of the trajectory with the horizontal plan (α) is between 20 and 45°. Thus, the result of this method is an interval of estimation in which the velocity is included.These methods do not give precise results. But these approximations supply valid informations on the velocity of extinct or living animals and can lead to the estimation of their maximum speed if the parameters E, T, vary. The problem of the errors is also discussed. It is shown that the errors in estimating the parameters have a no significant influence on the results.     

Influence du corps gras sur l'évolution de l'ovaire nymphal de Tenebrio molitor, étude en culture in vitro

In Tenebrio molitor, the fat body contains several factors able to control the evolution of the pupal ovary in vitro. One or several factors soluble in ether inhibit the initiation of oöcyte growth. One or several factors insoluble in ether stimulate the course of this growth, folicle organization, and the appearance of the transitory zone. The inhibitory action of the fat body is greatest towards the middle of the pharate adult stage. The stimulatory action of this organ is maximum at the beginning of pupal life and during the approach of adult ecdysis.     

L'âge du complexe récifal des côtes de meuse entre verdun et commercy et la stratigraphie de l'oxfordien dans l'est du bassin de Paris

The question of the age of «Hauts de Meuse coral rag is studied again using new faunas of brachiopods and ammonites collected above, below and inside the coral rag. Ammonites fauna of the well known «Calcaires de Creuë is revised. Results obtained agree closely.The «Hauts de Meuse coral rag grew up during Middle Oxfordian time; and extend part of the Plicatilis zone (Antecedens Subzone) and the whole Transversarium Zone up to the basis of the Bifurcatus Zone, for the first time identified in Lorraine. So it span a short depositional time, about 1 MY, with a high deposition rate, the thickness of the coral rag being more than 100 meters, reaching 150 meters in Saint-Mihiel area. Towards SE, on the other side of the Marne Gap, the sudden change into basinal facies go with a reducing of thickness and deposition rate.Upper Oxfordian deposits show a relative uniformity of facies and thickness. At the top of Middle Oxfordian limestones, the age of the discontinuity and the Sequanian renewal of deposition is basal Bifurcatus Zone. Erosional renewal and terrigeneous deposits are a wide spread event by the beginning of Upper Oxfordian.In the paleontological part, species upon which are based new ages and interpretations are only figured, except the species new or with new paleontological data, which are described. Type specimen of Perisphinctes mosensisBayle, from the «Calcaires de Creuë, is described and photographically figured for the first time.     

L'action exercée par l'appareil endocrine sur les phases tardives de l'ovogenèse chez Locusta migratoria

Most studies dealing with the endocrine control of oogenesis are focused on vitellogenesis, but the later events of oogenesis: vitelline membrane formation and choriogenesis have received less atention. In Locusta migratoria, the corpora allata evidently control vitellogenesis, but seem to have no bearing upon the formation of the vitelline membrane or the chorion. If the insects are deprived of their CA at the time when the oocyte length is around 3.5 to 4 mm, (normal length at egg laying: 6.5 mm) vitelline membrane formation and the chorion production are unaffected. In the same species, the electrocoagulation of the pars intercerebralis or excision of corpora cardiaca disturb oogenesis; vitelline membrane material is synthesized and secreted, but the primary granules fail to join each other and to transform into vitelline membrane and the production of chorionic material is disturbed.The rER of the follicle cell seems quite active but the substance elaborated accumulates in the Golgi saccules, and therefore the cytoplasm contains only very few secretory granules. The implantation of supernumerary pars intercerebralis and corpora cardiaca into immature females induces precocious deposition of the vitelline membrane which is complete in 5.0 mm oocytes (instead of 5.6 to 6.0 mm) and chorion formation is completed in 5.5 mm oocytes (instead of 6.0 to 6.3 mm). Ecdysone is secreted in the ovaries at the end of oogenesis. We tested the result of injecting ecdysone to immature females. It results in hastening oocyte growth during 24 hr, but this accelerated growth is followed by degeneration.     

La sculpture et l'infrastructure du sporoderme de Ginkgo biloba comparées à celles des enveloppes polliniques des cyccadales

The Ginkgo biloba sporoderm, when examined with transmission and scanning electron microscopes, shows a sculpture and a texture which are very different from those observed in cycads.The ornamental units are “rugules” which are evenly disperesed over the pollen grain surface except on the germinal zones which is decorated with “verrucoïde” components mixed with a few ridges. (2) The exine is composed of a tripartite sexine, the middle zone of which is full of irregular pits, and of a thick and strongly lamellated nexine.     

Scolécodontes,assemblage et appareil de vers annelidespolychetes errantes de l'aptien de la région d'Agadir,Maroc

From the Aptian scolecodonts inventory of the Agadir area one jaw apparatus and one joining of polychetes are reconstructed. One is the elementary species Schistomeringos expectatusSzaniawski & Gazdzicki, 1978, the oldest representative of the multielementary family Dorvilleidae Chamberlin 1919. The other is a representative of either the multielementary families Atraktoprionidae Kielan Jaworowska 1966 emend. Kozur, 1970 or Arabellidae Hartman 1944. This joining is considered as an hypothesis since all the necessary criteria, defined by H. Szaniawski & A. Gazdzicki 1978, are not all present.     

Analyse biostratigraphique et paléontologique de l'Albien marin d'Estremadura (Portugal)

The marine Albain from Estremadura is splited up into four Orbitolinidae zones, adjusted to biostratigraphic scales from mesogean province: zone with Orbitolina (Mesorbitolina) minuta (lower to middle Albian); zone with Neorbitolinopsis conulus (base of upper Albian); zone with Orbitolina (Orbitolina) concava (top of upper Albian) and zone with Orbitolina (Orbitolina) duranddelgai (Vraconian). The vertical extension of the main fossils is replaced in the framework of these zones. The most interesting species for biostratigraphy, paleoecology or taxinomy are summarily described.     

Les vertébrés dévoniens de l'Iran central I: Dipneustes

The dipnoan dental plates discovered in the Frasnian of the Kerman region (Central Iran) are referred to the genera Rhinodipterus, ? Dipterus and ? Chirodipterus. The new taxon Iranorhynchus seyedemamii n. g., n. sp. is erected for a peculiar lower jaw with an elongate, spatula-shaped symphysial region, and with all the ventral and lateral dermal bones fused into a continuous exoskeleton. Furthermore, this lower jaw possesses a well-developed system of symphysial tubuli which is related to the bottle-shaped cavities of the exoskeleton (pore-canal system). A snout fragment from a large dipnoan is provisionnally referred to a rhynchodipterid.     

'Etude comparée des genres Babinka Barrandeet Coxiconcha Babin (Mollusques Bivalves de l'Ordovicien) intérêt phylogénétique

The comparative examination of two genera ofMollusca Bivalvia from the Lower Ordovician, Babinka and Coxiconcha, reveals some affinities between them. Their similarities are relating to the arrangement of the muscle scars; the dentitions are, on the contrary, rather unlike; a similar origin might nevertheless be advanced. It is pointed out that Coxiconcha seems, moreover, to be a primitive Modiomorphid. These new data allow to perfect the previous phylogenetic sketches and consequently to correct the classification, making obvious the outstanding part of the Actinodontoids in the diversification of the Mollusca Bivalvia during the Ordovician.     

Cycles embryonnaires et sécrétion de la cuticule chez l'embryon de blatte, Blabera craniifer

Cuticle deposition has been studied with the electron microscope in cockroach embryos (Blabera craniifer) during normal incubation in situ and in culture in vitro, in the absence or presence of inokosterone (a phytoecdysone).Two cuticles are deposited successively during embryonic life, respectively between stages 11 and 17, and stage 21 and 24 hr after hatching. The occurrence of two embryonic cycles is thus demonstrated, the first ending at stage 17 without exuviation since there is no old cuticle to be shed, the second one at hatching.In embryos explanted at stage 17 and cultured in vitro, the formation of cuticle 2 occurred at the same rate as in situ. The addition of inokosterone (50 μg/ml) to the medium resulted in the early onset of cuticle deposition (in 3 days as compared with 15 days in situ) in legs previously cut at the base of the tarsus. Cuticle 2 was completed within 9 days after explantation (as compared with about 20 days elapsing in the normal embryo between stage 17 and the completion of cuticle 2). Unsectioned appendages were insensitive to the hormone.Regeneration of sectioned legs, which occurred normally n vitro in non-treated embryos, was completely inhibited in the presence of inokosterone, presumably because the hormone caused early immobilization of cells through accelerated cuticle formation.Results suggest that embryonic cycles are controlled by the same hormonal mechanism as larval cycles.     

Réflexions critiques sur l'écologieet la systématique des Lingules actuelles et fossiles

New topics on ecology and systematics of recent and fossil Lingulids lead to an obvious revision of our knowledges on this zoological group. At first, the recent species need systematics and taxonomy on the bases of new described specific criteria (as, morphology of deltidial areas, muscle disposition); the results are briefly indicated. But, in fossil species, disorder and disparity of used characteristics are emphasized.The general conceiving on ecology of Lingulids are reviewed and discussed, especially on bathymetry and salinity; sediment and oxygenation conditions; taphocoenosis and lingulid «communities. On recent species, all these points are also studied, especially some ecological requirements (salinity, bathymetry, grain size), and mechanism of burrowing ability, burrow living positions in the sediments, as shell preservations after death and fossilization, to facilitate the paleobiotope interpretations. Recent animals are euryhalin, surviving at salinities from about 13 to 42‰; they could be considered as well adapted to waters with strong salinity fluctuations. They show preference to fine sand bottoms (lowest particle size about 40–60 μm). Their bathymetric distribution occurs between 0 and about 500 m (Lingula especially between 5–50 m; Glottidia 15–70 m). The isotherms 8–10°C seem to restrict their geographical and bathymetric distribution.Therefore, some post-palaeozoic lingulid bedsare studied or redescribed on the bases of the above discussed characteristics, and new interpretations on the environmental situation are given (Trias of Vosges Mountains; Oligocene from Japan; Eocene of London Basin). More caution must be used in study of fossil Lingulids that are not especially animals living in infralittoral bottoms with low salinity and deficient oxygenation, as generally accepted.     

Nanisme écologique de deux espèces d'échinides irréguliers dans l'Hauterivien de la région de Castellane (Alpes-de-Haute-Provence)

Three species of irregular see-urchins form the settlement of a marly level (lower Hauterivian) of the Castellane area. Two of them are strongly smaller than the norm. Their stunting is prouved by ontogenic, mecanical, sedimentological and ecological arguments. The ecological grounds of stunting are discussed.