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1.
A. V. Zlatska 《Russian Journal of Genetics》2005,41(8):823-834
The results of studies on genetic control of the grain protein content in common wheat and the attempts of its improvement using traditional breeding approaches and methods of experimental mutagenesis and introgressive hybridization are presented. The evolution of the views on the genetic structure of the character is considered. Possible explanations are given to the fact that the attempts to significantly improve the character in question in common wheat cultivars have failed in spite of long-term genetic and selection studies. Possible lines of further investigation of genetic control of grain protein content and its increase in common wheat are discussed.__________Translated from Genetika, Vol. 41, No. 8, 2005, pp. 1013–1026.Original Russian Text Copyright © 2005 by Zlatska. 相似文献
2.
Identification of the genetic controlling system of the protein content in the grain of winter wheat
L. L. Lysa 《Cytology and Genetics》2009,43(4):258-261
The additive-dominant system of genetic control of the protein content in the grain of winter wheat was established. A leading role of additive effects in the expression of the genes controlling the protein content was demonstrated. The intermediate type of inheritance of the grain’s level of protein content in hybrids was shown. 相似文献
3.
The blue grain trait in common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD), which is caused by blue pigments in the aleurone layer, was originally derived from the tall wheatgrass (Thinopyrum ponticum Liu & Wang = Agropyron elongatum, 2n = 10x = 70, StStStStEeEeEbEbEXEx) during chromosome engineering research. Over the last few decades, there have been continued interests in the genetic mechanism of this blue coloration and the practical utilization of the blue aleurone character as a phenotypic marker. This article reviews the research history and the recent progress of the studies on blue-grained wheat, with emphases on genetic and biochemical analysis and practical applications of blue-grained wheat. 相似文献
4.
M. Rapp V. Lein F. Lacoudre J. Lafferty E. Müller G. Vida V. Bozhanova A. Ibraliu P. Thorwarth H. P. Piepho W. L. Leiser T. Würschum C. F. H. Longin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(6):1315-1329
Key message
Simultaneous improvement of protein content and grain yield by index selection is possible but its efficiency largely depends on the weighting of the single traits. The genetic architecture of these indices is similar to that of the primary traits.Abstract
Grain yield and protein content are of major importance in durum wheat breeding, but their negative correlation has hampered their simultaneous improvement. To account for this in wheat breeding, the grain protein deviation (GPD) and the protein yield were proposed as targets for selection. The aim of this work was to investigate the potential of different indices to simultaneously improve grain yield and protein content in durum wheat and to evaluate their genetic architecture towards genomics-assisted breeding. To this end, we investigated two different durum wheat panels comprising 159 and 189 genotypes, which were tested in multiple field locations across Europe and genotyped by a genotyping-by-sequencing approach. The phenotypic analyses revealed significant genetic variances for all traits and heritabilities of the phenotypic indices that were in a similar range as those of grain yield and protein content. The GPD showed a high and positive correlation with protein content, whereas protein yield was highly and positively correlated with grain yield. Thus, selecting for a high GPD would mainly increase the protein content whereas a selection based on protein yield would mainly improve grain yield, but a combination of both indices allows to balance this selection. The genome-wide association mapping revealed a complex genetic architecture for all traits with most QTL having small effects and being detected only in one germplasm set, thus limiting the potential of marker-assisted selection for trait improvement. By contrast, genome-wide prediction appeared promising but its performance strongly depends on the relatedness between training and prediction sets.5.
LIN WANG FA CUI JINPING WANG LI JUN ANMING DING CHUNHUA ZHAO XINGFENG LI DESHUN FENG JURONG GAO HONGGANG WANG 《Journal of genetics》2012,91(3):303-312
Grain protein content in wheat (Triticum aestivum L.) is generally considered a highly heritable character that is negatively correlated with grain yield and yield-related traits. Quantitative trait loci (QTL) for protein content was mapped using data on protein content and protein content conditioned on the putatively interrelated traits to evaluate possible genetic interrelationships between protein content and yield, as well as yield-related traits. Phenotypic data were evaluated in a recombinant inbred line population with 302 lines derived from a cross between the Chinese cultivar Weimai 8 and Luohan 2. Inclusive composite interval mapping using IciMapping 3.0 was employed for mapping unconditional and conditional QTL with additives. A strong genetic relationship was found between protein content and grain yield, and yield-related traits. Unconditional QTL mapping analysis detected seven additive QTL for protein content, with additive effects ranging in absolute size from 0.1898% to 0.3407% protein content, jointly accounting for 43.45% of the trait variance. Conditional QTL mapping analysis indicated two QTL independent from yield, which can be used in marker-assisted selection for increasing yield without affecting grain protein content. Three additional QTL with minor effects were identified in the conditional mapping. Of the three QTLs, two were identified when protein content was conditioned on yield, which had pleiotropic effects on those two traits. Conditional QTL mapping can be used to dissect the genetic interrelationship between two traits at the individual QTL level for closely correlated traits. Further, conditional QTL mapping can reveal additional QTL with minor effects that are undetectable in unconditional mapping. 相似文献
6.
The genetic control of grain hardness and its association with the specific friabilin content on starch granules of common wheat cultivars and lines with intervarietal substitutions of homeologous group-5 chromosomes were studied. A significant correlation was revealed between the technological parameters of grain hardness (mean size of flour particles) and the specific content of puroindolines on the starch surface estimated in terms of starch doses. The results obtained allowed the method of starch doses to be used to identify soft and hard wheat cultivars and lines based on an analysis of a single grain. The biochemical analysis confirmed the previously obtained estimates of flour-grinding properties of wheat cultivars and substitution lines and allowed specific genotypes to be characterized according to the composition of puroindolines. The influence of chromosomes 5D and 5A of donor wheat cultivars on the activity of the Ha loci of recipient cultivars was revealed and found to be associated with the composition of PIN products and with the expression of the Pina-D1 and Pinb-D1 genes. 相似文献
7.
Wheat is one of the most important global crops and selection for better performance has been ongoing since ancient times. As a quantitative trait controlled by the interplay of several genomic loci and under the strong influence of the environment, grain protein content (GPC) is of major interest in breeding programs. Here, we review the most recent contributions to the genetics underlying wheat GPC and grain protein deviation (GPD, representing the relationship between grain protein content and yield), together with the performance of genomic prediction models characterizing these traits. A total of 364 significant loci related to GPC and GPD are positioned on the hexaploid wheat genome, highlighting genomic regions where significant independent QTL overlap, with special focus on two regions located on chromosomes 3A and 5A. Some of the corresponding homoeologous sequences co-locate with significant independent QTL reported on the B and D subgenomes. Overlapping independent QTL from different studies are indicative of genomic regions exhibiting stability across environments and genotypes, with promising candidates for improving grain quality. 相似文献
8.
Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits 总被引:2,自引:0,他引:2
Dingbo Zhang Xiaoge Zhao Xuemin Cao Lingli Dong Jinxing Liu Kunling Chen Huawei Zhang Caixia Gao Daowen Wang 《The Plant journal : for cell and molecular biology》2018,94(5):857-866
GW2 is emerging as a key genetic determinant of grain weight in cereal crops; it has three homoeologs (TaGW2‐A1, ‐B1 and ‐D1) in hexaploid common wheat (Triticum aestivum L.). Here, by analyzing the gene editing mutants that lack one (B1 or D1), two (B1 and D1) or all three (A1, B1 and D1) homoeologs of TaGW2, several insights are gained into the functions of TaGW2‐B1 and ‐D1 in common wheat grain traits. First, both TaGW2‐B1 and ‐D1 affect thousand‐grain weight (TGW) by influencing grain width and length, but the effect conferred by TaGW2‐B1 is stronger than that of TaGW2‐D1. Second, there exists functional interaction between TaGW2 homoeologs because the TGW increase shown by a double mutant (lacking B1 and D1) was substantially larger than that of their single mutants. Third, both TaGW2‐B1 and ‐D1 modulate cell number and length in the outer pericarp of developing grains, with TaGW2‐B1 being more potent. Finally, TaGW2 homoeologs also affect grain protein content as this parameter was generally increased in the mutants, especially in the lines lacking two or three homoeologs. Consistent with this finding, two wheat end‐use quality‐related parameters, flour protein content and gluten strength, were considerably elevated in the mutants. Collectively, our data shed light on functional difference between and additive interaction of TaGW2 homoeologs in the genetic control of grain weight and protein content traits in common wheat, which may accelerate further research on this important gene and its application in wheat improvement. 相似文献
9.
烯效唑干拌种对小麦氮素积累和运转及籽粒蛋白质品质的影响 总被引:1,自引:0,他引:1
通过田间试验,研究了不同烯效唑干拌种剂量对3个不同筋力小麦品种植株氮素积累、运转和籽粒蛋白质品质的影响,结果表明,基因型、环境及烯效唑处理对小麦品质的影响效应依次减小,且均达到了极显著水平,但三者的互作效应较小。烯效唑处理后提高了不同生态点下不同小麦品种籽粒蛋白质含量和产量,处理后的面筋含量和沉淀值增加,面团形成时间和稳定时间延长;干拌种增加了开花期各营养器官中的氮素含量和单株氮素积累量,花后氮素总转移量、总转移率及其对籽粒氮的贡献率极显著提高,且处理后旗叶中可溶性蛋白质含量在花后15 d内均显著高于对照;对籽粒中氮含量而言,烯效唑处理后提高了灌浆初期籽粒中的非蛋白氮含量,花后5—20 d内均高于对照,灌浆期间籽粒蛋白氮含量均高于对照,因而处理后的粗蛋白质含量变化动态特点为谷底高、回升快。研究认为,烯效唑处理如同基因、环境一样独立影响小麦籽粒品质,而烯效唑处理后提高了开花初期旗叶中的可溶性蛋白质含量和花前营养器官中氮素含量及花后氮素转运量,可能是其提高籽粒非蛋白氮含量、促进籽粒蛋白质含量增加和蛋白质质量提高的重要原因之一,烯效唑干拌种对小麦籽粒蛋白质品质的改善具有广适性。 相似文献
10.
Toi J. Tsilo Gary A. Hareland Senay Simsek Shiaoman Chao James A. Anderson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(4):717-730
Kernel characteristics, particularly kernel weight, kernel size, and grain protein content, are important components of grain
yield and quality in wheat. Development of high performing wheat cultivars, with high grain yield and quality, is a major
focus in wheat breeding programs worldwide. Here, we report chromosome regions harboring genes that influence kernel weight,
kernel diameter, kernel size distribution, grain protein content, and grain yield in hard red spring wheat breeding lines
adapted to the Upper Midwest region of the United States. A genetic linkage map composed of 531 SSR and DArT marker loci spanned
a distance of 2,505 cM, covering all 21 chromosomes of wheat. Stable QTL clusters influencing kernel weight, kernel diameter,
and kernel size distribution were identified on chromosomes 2A, 5B, and 7A. Phenotypic variation explained by individual QTL
at these clusters varied from 5 to 20% depending on the trait. A QTL region on chromosome 2B confers an undesirable pleiotropic
effect or a repulsion linkage between grain yield (LOD = 6.7; R
2 = 18%) and grain protein content (LOD = 6.2; R
2 = 13.3%). However, several grain protein and grain yield QTL independent of each other were also identified. Because some
of the QTL identified in this study were consistent across environments, DNA markers will provide an opportunity for increasing
the frequency of desirable alleles through marker-assisted selection. 相似文献
11.
Merav Chatzav Zvi Peleg Levent Ozturk Atilla Yazici Tzion Fahima Ismail Cakmak Yehoshua Saranga 《Annals of botany》2010,105(7):1211-1220
Background and Aims
Micronutrient malnutrition, particularly zinc and iron deficiency, afflicts over three billion people worldwide due to low dietary intake. In the current study, wild emmer wheat (Triticum turgidum ssp. dicoccoides), the progenitor of domesticated wheat, was tested for (1) genetic diversity in grain nutrient concentrations, (2) associations among grain nutrients and their relationships with plant productivity, and (3) the association of grain nutrients with the eco-geographical origin of wild emmer accessions.Methods
A total of 154 genotypes, including wild emmer accessions from across the Near Eastern Fertile Crescent and diverse wheat cultivars, were characterized in this 2-year field study for grain protein, micronutrient (zinc, iron, copper and manganese) and macronutrient (calcium, magnesium, potassium, phosphorus and sulphur) concentrations.Key Results
Wide genetic diversity was found among the wild emmer accessions for all grain nutrients. The concentrations of grain zinc, iron and protein in wild accessions were about two-fold greater than in the domesticated genotypes. Concentrations of these compounds were positively correlated with one another, with no clear association with plant productivity, suggesting that all three nutrients can be improved concurrently with no yield penalty. A subset of 12 populations revealed significant genetic variation between and within populations for all minerals. Association between soil characteristics at the site of collection and grain nutrient concentrations showed negative associations between soil clay content and grain protein and between soil-extractable zinc and grain zinc, the latter suggesting that the greatest potential for grain nutrient minerals lies in populations from micronutrient-deficient soils.Conclusions
Wild emmer wheat germplasm offers unique opportunities to exploit favourable alleles for grain nutrient properties that were excluded from the domesticated wheat gene pool. 相似文献12.
小麦籽粒中植酸、戊聚糖含量及其与相关性状关系的研究 总被引:4,自引:0,他引:4
选用不同基因型小麦,测定了籽粒中植酸、蛋白质及戊聚糖的含量,并对其进行遗传相关分析,结果表明:(1)各性状在品种间存在显著性差异,且植酸的广义遗传力比较低;(2)植酸含量与蛋白质含量呈极显著的正相关,与戊聚糖呈极显著负相关。通过对参试的18个不同基因型小麦中植酸和戊聚糖含量进行聚类分析,可以将18个基因型小麦聚为四类,并初步认为豫麦47是参试品种中最适宜于用作饲用小麦。 相似文献
13.
强筋与弱筋小麦籽粒蛋白质组分与加工品质对灌浆期弱光的响应 总被引:5,自引:0,他引:5
选用强筋小麦品种济麦20和弱筋小麦品种山农1391,在大田试验条件下,分别于籽粒灌浆前期(花后6—9 d)、中期(花后16—19 d)和后期(花后26—29 d)对小麦进行弱光照处理,研究了籽粒产量、蛋白质组分及加工品质的变化。灌浆期弱光显著降低小麦籽粒产量,灌浆中期对济麦20和灌浆后期对山农1391的产量降幅最大。弱光处理后,籽粒氮素积累量及氮素收获指数减少。但弱光使籽粒蛋白质含量显著升高,其中灌浆中期弱光升幅最大,原因可能是由于其粒重降低造成的。弱光对可溶性谷蛋白无显著影响,但增加不溶性谷蛋白含量,使谷蛋白聚合指数显著升高,面团形成时间和稳定时间亦升高,籽粒灌浆中、后期弱光对上述指标的影响较前期大。灌浆期短暂的弱光照对改善强筋小麦粉质仪参数有利,但使弱筋小麦变劣;并均伴随籽粒产量的显著降低这一不利影响。 相似文献
14.
Hua Chen Kassa Semagn Muhammad Iqbal Neshat Pazooki Moakhar Teketel Haile Amidou N’Diaye Rong-Cai Yang Pierre Hucl Curtis Pozniak Dean Spaner 《Molecular breeding : new strategies in plant improvement》2017,37(11):141
We recently reported genomic regions associated with resistance to four wheat diseases and insensitivity to three Pyrenophora tritici-repentis toxins in an association mapping panel consisting of 81 diverse Canadian western spring wheat (Triticum aestivum L.) cultivars. Here, we report genomic regions and SNPs associated with days to heading, plant maturity, plant height, test weight (grain volume weight), grain yield, and grain protein content in the same population using genome-wide association studies (GWAS). The 81 spring wheat cultivars were evaluated for the above six traits across six environments and genotyped with 19,919 polymorphic SNPs and 14 gene-specific markers. Using mixed liner model and a threshold of p ≤ 3.1 × 10?4, we identified a total of 139 significant marker-trait associations that were mapped at 19 genomic regions on 11 chromosomes for heading (3 regions), maturity (2), plant height (3), test weight (3), grain yield (6), and grain protein (2). Each region consisted of clusters of markers ranging from 2 to 33 and individually explained from 4.5 to 26.1% of the phenotypic variation averaged over six environments. Some the genomic regions identified in the present study are novel, while others, such as the regions for grain protein on 1B, days to heading on 5A, plant height on 4B, and test weight on 7A, were located close to either known genes or QTLs reported in previous studies, but direct comparisons in some cases were challenging due to lack of common set of markers and reliable physical positions among the different studies. Results from this study provide additional information to wheat researchers developing improved spring wheat cultivars. 相似文献
15.
The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat 总被引:1,自引:0,他引:1
High grain protein content (GPC) is a frequent target of wheat breeding programmes because of its positive effect on bread and pasta quality. A wild wheat allele at the Gpc-B1 locus with a significant impact on this trait was identified previously. The precise mapping of several senescence-related traits in a set of tetraploid recombinant substitution lines (RSLs) segregating for Gpc-B1 is reported here. Flag leaf chlorophyll degradation, change in peduncle colour, and spike water content were completely linked to the Gpc-B1 locus and to the differences in GPC within a 0.3 cM interval corresponding to a physical distance of only 250 kb. The effect of Gpc-B1 was also examined in different environments and genetic backgrounds using a set of tetraploid and hexaploid pairs of isogenic lines. The results were consistent with those observed in the RSLs. The high GPC allele conferred a shorter duration of grain fill due to earlier flag leaf senescence and increased GPC in all four genetic backgrounds. The effect on grain size was more variable, depending on the genotype-environment combinations. These results are consistent with a model in which the wild-type allele of Gpc-B1 accelerates senescence in flag leaves producing pleiotropic effects on nitrogen remobilization, total GPC, and grain size. 相似文献
16.
Fait VI 《T?Sitologii?a i genetika》2003,37(5):57-64
Hypotheses of differences in genetic control on duration of vernalization for winter wheat have been considered. It has been shown that differences in this character are controlled by independent Vrd gene system. Monogenic dominant near-isogenic lines have been created. They differ in two non-allelic Vrd1 and Vrd2 genes with unequal expression. The response of created lines to photoperiodic changes and Vrd genes effects on various agronomic traits have been characterized. Vrd genotypes have been identified in a number of winter common wheat cultivars. 相似文献
17.
Genetic analysis of grain protein-content,grain yield and thousand-kernel weight in bread wheat 总被引:21,自引:0,他引:21
Groos C Robert N Bervas E Charmet G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(6):1032-1040
Grain yield and grain protein content are two very important traits in bread wheat. They are controlled by genetic factors, but environmental conditions considerably affect their expression. The aim of this study was to determine the genetic basis of these two traits by analysis of a segregating population of 194 F(7) recombinant inbred lines derived from a cross between two wheat varieties, grown at six locations in France in 1999. A genetic map of 254 loci was constructed, covering about 75% of the bread wheat genome. QTLs were detected for grain protein-content (GPC), yield and thousand-kernel weight (TKW). 'Stable' QTLs (i.e. detected in at least four of the six locations) were identified for grain protein-content on chromosomes 2A, 3A, 4D and 7D, each explaining about 10% of the phenotypic variation of GPC. For yield, only one important QTL was found on chromosome 7D, explaining up to 15.7% of the phenotypic variation. For TKW, three QTLs were detected on chromosomes 2B, 5B and 7A for all environments. No negative relationships between QTLs for yield and GPC were observed. Factorial Regression on GxE interaction allowed determination of some genetic regions involved in the differential reaction of genotypes to specific climatic factors, such as mean temperature and the number of days with a maximum temperature above 25 degrees C during grain filling. 相似文献
18.
Grain zinc, iron and protein concentrations and zinc-efficiency in wild emmer wheat under contrasting irrigation regimes 总被引:1,自引:0,他引:1
Zvi Peleg Yehoshua Saranga Atilla Yazici Tzion Fahima Levent Ozturk Ismail Cakmak 《Plant and Soil》2008,306(1-2):57-67
Micronutrient malnutrition, and particularly deficiency in zinc (Zn) and iron (Fe), afflicts over three billion people worldwide, and nearly half of the world’s cereal-growing area is affected by soil Zn deficiency. Wild emmer wheat [Triticum turgidum ssp. dicoccoides (Körn.) Thell.], the progenitor of domesticated durum wheat and bread wheat, offers a valuable source of economically important genetic diversity including grain mineral concentrations. Twenty two wild emmer wheat accessions, representing a wide range of drought resistance capacity, as well as two durum wheat cultivars were examined under two contrasting irrigation regimes (well-watered control and water-limited), for grain yield, total biomass production and grain Zn, Fe and protein concentrations. The wild emmer accessions exhibited high genetic diversity for yield and grain Zn, Fe and protein concentrations under both irrigation regimes, with a considerable potential for improvement of the cultivated wheat. Grain Zn, Fe and protein concentrations were positively correlated with one another. Although irrigation regime significantly affected ranking of genotypes, a few wild emmer accessions were identified for their advantage over durum wheat, having consistently higher grain Zn (e.g., 125 mg kg?1), Fe (85 mg kg?1) and protein (250 g kg?1) concentrations and high yield capacity. Plants grown from seeds originated from both irrigation regimes were also examined for Zn efficiency (Zn deficiency tolerance) on a Zn-deficient calcareous soil. Zinc efficiency, expressed as the ratio of shoot dry matter production under Zn deficiency to Zn fertilization, showed large genetic variation among the genotypes tested. The source of seeds from maternal plants grown under both irrigation regimes had very little effect on Zn efficiency. Several wild emmer accessions revealed combination of high Zn efficiency and drought stress resistance. The results indicate high genetic potential of wild emmer wheat to improve grain Zn, Fe and protein concentrations, Zn deficiency tolerance and drought resistance in cultivated wheat. 相似文献
19.
Identification of quantitative trait locus of zinc and phosphorus density in wheat (Triticum aestivum L.) grain 总被引:1,自引:0,他引:1
Rongli Shi Hongwei Li Yiping Tong Ruilian Jing Fusuo Zhang Chunqin Zou 《Plant and Soil》2008,306(1-2):95-104
Zinc (Zn) is an essential micronutrient for human beings. However, Zn malnutrition has become a major problem throughout the
world. Wheat is the most important food crop in the world, therefore, developing Zn-enriched wheat varieties provides an effective
approach to reduce Zn malnutrition in human beings. The aim of this study was to understand the genetic control of grain Zn
density in wheat and hence, to provide genetic basis for breeding wheat with high grain Zn density using molecular approach.
A doubled haploid (DH) population developed from a cross between winter wheat varieties Hanxuan10 and Lumai 14 was used to
identify quantitative trait loci (QTLs) for Zn concentration and content in wheat grains. In addition, phosphorus (P) concentration
and content in wheat grain were also investigated to examine possible interactions between these two nutrients. The wheat
grains used in this study were harvested from the plants grown under normal condition in a field trial. We found the grain
Zn concentrations of the DH population varied from 25.9 to 50.5 mg/kg and the Zn content varied from 0.90 to 2.21 μg/seed.
The grain P concentrations of the DH population varied from 0.258 to 0.429 mg/kg, and the P contents varied from 0.083 to
0.186 mg/seed. A significant positive correlation was observed between Zn and P density in this experiment. The results showed
that both grain Zn and P densities were controlled by polygenes. Four and seven QTLs for Zn concentration and Zn content were
detected, respectively. All the four QTLs for Zn concentration co-located with the QTLs for Zn content, suggesting a possibility
to improve both grain Zn concentration and content simultaneously. Four and six QTLs for P concentration and P content were
detected, respectively. The two QTLs for grain Zn concentration on chromosomes 4A and 4D co-located with the QTLs for P concentration.
The four QTLs for grain Zn content on chromosome 2D, 3A and 4A co-located with the QTLs for P contents, reflecting the positive
correlations between the grain Zn and P density, and providing possibility of improving grain micro- and macronutrient density
simultaneously in wheat. In order to improve human health, the effect of P–Zn relation in grain on the Zn bioavailability
should also be considered in the future work. 相似文献
20.
施氮水平对高产麦田土壤硝态氮时空变化及氨挥发的影响 总被引:12,自引:1,他引:12
研究了不同施氮水平对高产麦田土壤硝态氮时空变化和氨挥发的影响.结果表明,高产麦田土壤硝态氮在播种至冬前阶段不断向深层移动,并在140cm以下土层积累.施纯氮96~168 kg·hm-2处理,增加了60 cm以上土层土壤硝态氮含量,降低了土壤氮素表观损失量占施氮量的比例,提高了小麦籽粒蛋白质含量和籽粒产量,且土壤氨挥发损失较低,基施氮氨挥发损失占基施氮量的4.23%~5.51%;施氮量超过240 kg N·hm-2,促进了土壤硝态氮向深层的移动和积累,基施氮氨挥发损失、土壤氮素表观损失量及其占施氮量的比例均显著升高,对小麦籽粒蛋白质含量无显著影响,但籽粒产量降低.高产麦田适宜的氮素用量为132~204 kg N·hm-2. 相似文献