Elektronenmikroskopische Untersuchungen an Mycobacterium phlei

Summary The structure and formation of polyphosphate-granules has been investigated by means of ultrathin sections through Mycobacterium phlei.After removing the polyphosphate, a fine grained and scarcely electron-scattering structure was to be seen which, however, was not bordered by a membrane.The granules probably originate in distinct regions of the cytoplasm from irregularly shaped intermediate stages or from agglomerations of micro-granules.Mitochondria-like structures of Mycobacterium phlei are described. Polyphosphates probably may be accumulated in these structures as well.     

Myxoxanthophyll als Hauptcarotinoid von Mycobacterium phlei

Zusammenfassung In einem carotinoidreichen Stamm von Mycobacterium phlei wurde neben den Carotinoiden Phytoen, Phytofluen, -Carotin, Neurosporin, Lycopin, - und -Carotin ein Oxycarotinoid aufgefunden, das mit dem Blaualgenpigment Myxoxanthophyll (=Aphanizophyll) identisch zu sein scheint. Der Gehalt an Carotinoiden beträgt 1% des Trockengewichtes der Bakterien; davon liegen 95% in Form des Oxycarotinoids vor. Myxoxanthophyll wurde aus Tetrahydrofuran-Petroläther mehrmals umkristallisiert und charakterisiert.Herrn Professor Dr. Dr. h. c. A. Rippel zum 70. Geburtstag.     

Multiple cytochromes b in Mycobacterium phlei

Electron transport particles from $\text{M. phlei}$ contain at least 3 different active forms of cytochrome b, one reduced by NADH, with a λ_max at 563 nm (b_N563), and the other two reduced by either succinate or NADH, with λ_max at 559 and 563 nm (b_S559) and (b_S563). Low temperature λ_max for cytochrome b reduction with NADH or succinate are described. During steady state only b_S563 was observed with succinate. In the presence of ATP, succinate reduced an increased amount of a b563. A branching of the NAD⁺-linked pathway and a convergence at the level of cytochrome c is suggested, with only one branch accessible to succinate.