共查询到20条相似文献,搜索用时 15 毫秒
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In cultured primary hepatocytes UDP-glucuronosyltransferase form 1A2 (UGT1A2) mRNA level is 80 times higher than that found in rat liver. We previously identified an enhancer sequence in the UGT1A2 promoter, and designated it as culture-associated expression responsive enhancer module (CEREM). Affinity chromatography with DNA fragments containing CEREM allowed enrichment of nuclear factor I (NFI) proteins from cultured hepatocytes. The NFI family is encoded by four distinct genes, NFI-A, NFI-B, NFI-C, and NFI-X. Immunoblot analysis with isoform-specific antibodies showed that NFI-A1 existed as a major component in rat liver and cultured hepatocytes. By contrast, NFI-C1 was present in rat liver but disappeared immediately upon cultivation of hepatocytes. Only trace amounts of NFI-B and NFI-X were detectable in rat liver and cultured hepatocytes. NFI-A1 elevated expression of the reporter gene that is under the control of CEREM, while NFI-C1 had an inhibitory effect. Co-expression of a constant amount of NFI-A1 with an increasing amount of NFI-C1 led to a concentration-dependent decrease in the expression of the CEREM-controlled reporter gene mediated by NFI-A1. Activation of UGT1A2 expression by NFI-A1 is suppressed by the coexistence of NFI-C1 in the liver, and culture-associated expression of UGT1A2 is triggered by the rapid disappearance of NFI-C1 in cultured hepatocytes. 相似文献
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Gronostajski RM 《Gene》2000,249(1-2):31-45
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Ling G Hauer CR Gronostajski RM Pentecost BT Ding X 《The Journal of biological chemistry》2004,279(27):27888-27895
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Expression level-dependent contribution of glucocorticoid receptor domains for functional interaction with STAT5 下载免费PDF全文
Doppler W Windegger M Soratroi C Tomasi J Lechner J Rusconi S Cato AC Almlöf T Liden J Okret S Gustafsson JA Richard-Foy H Starr DB Klocker H Edwards D Geymayer S 《Molecular and cellular biology》2001,21(9):3266-3279
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Transcription factor NFIC undergoes N-glycosylation during early mammary gland involution 总被引:3,自引:0,他引:3
Kane R Murtagh J Finlay D Marti A Jaggi R Blatchford D Wilde C Martin F 《The Journal of biological chemistry》2002,277(29):25893-25903
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