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1.
Diaconu CC Bleotu C Chivu M Alexiu I Petrusca D Anton G Achim R Ruta SM Cernescu C 《Journal of cellular and molecular medicine》2004,8(1):93-101
There are few information concerning the changes associated with the transition interval when slow growing, primary explanted human cancer cells are displaced by new selected faster growing cells and became an immortal cell line. In a previous paper ( J. Cell. Mol. Med. , 5 : 49–59, 2001) we described the TV cell line derived from a laryngeal tumor which harbors human papillomavirus (HPV) gene sequences throughout more than sixty in vitro passages. In this paper we analyze the modifications observed during the crisis interval when significant amount of cells senesce but occasional cells acquire some mutations that make them immortal. Confocal microscopy analysis revealed the heterogeneity of the cells in terms of their size and nucleus/cell ratio. Proliferation capacity was assessed by flow cytometry analyzing DNA content and expression of transferrin receptor (CD71). We discussed the possibility that HPV genome sequences alleviate a proliferation block during the crisis growth arrest of human larynx carcinoma cell line and the possibility that the cells monitor their size and growth by measuring the levels of some protein whose synthesis is coupled to cell development. 相似文献
2.
Cell size distributions, obtained either as protein distribution by flow cytometry or as cell volume distribution by a Coulter counter, give relevant information about the growth conditions of populations of budding yeast Saccharomyces cerevisiae. We have previously found a good correlation between these distributions and the growth rate in continuous cultures (Ranzi et al., Biotechnol. Bioeng. 1986, 28, 185-190). We now present determinations of the protein distributions and cell volume distributions during different fed-batch fermentations performed with a simple on/off controller. Since during the fed-batch fermentation a true steady state is not obtained, the distributions continuously change with time, but nevertheless we observed a good correlation between the average of both distributions and the actual growth rate. The behavior of the cell size distributions can be interpreted on the basis of a two-threshold cell cycle model in which both the critical protein content at budding (Ps) and the critical protein content for cell division (Pm) are differently modulated by the growth rate. Additional findings will be presented showing that this model can be used to successfully explain the insurgence and the maintenance of oscillatory states in continuous cultures. 相似文献
3.
DNA content analysis of insect cell lines by flow cytometry 总被引:1,自引:0,他引:1
The DNA content of insect cell lines (6 lepidoptera, 1 coleoptera and 1 diptera) was determined by flow cytometry. The DNA
profiles of the 8 cell lines tested were different. They were characterized by the presence of several peaks (2 to 7) corresponding
to different ploidy levels, by differences in the fluorescence intensity of each peak and by the proportion of cells in each
peak. Two cell lines (Cf124 and BmN) were constituted of 2 distinct populations of cells. The DNA profiles of the cell lines
were stable among the passages and during the length of time culture. This technique was demonstrated to be useful for the
detection of mixed cell lines and nucleopolyhedrovirus cell infection, using Autographa californica MNPV. The flow cytometry
gives interesting results on the cell cycle and the ploidy level; it appears as a good tool for insect cell lines characterization.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
4.
To improve the ability of flow cytometry to detect multidrug-resistant cells, we studied the extent to which cell volume heterogeneity accounts for the variance of intracellular daunorubicin (DNR) content. For P388 murine or HL-60 human leukemia cells exposed to DNR (1 micrograms/ml, 60 min), log intracellular DNR content varied in direct proportion to log cell volume measured by flow cytometry, with a correlation coefficient of .9. This relationship was confirmed by cell sorting based on intracellular DNR content with subsequent volume determination of the sorted cells. Normalization of intracellular DNR content for cell volume (thus obtaining intracellular DNR concentration) was accomplished by subtracting log cell volume from log intracellular DNR content for each cell. This resulted in a 34% decrease (range 23-58%) in standard deviation compared to DNR content measurements without volume normalization for all cell types tested. Following exposure to DNR (as above), intracellular DNR content of drug-sensitive P388 or HL-60 cells measured by flow cytometry was 12- and 8-fold greater than that of the multidrug-resistant sublines P388/ADR and HL-60/AR, respectively. However, because of the variance of intracellular DNR content, the predictive value of flow-cytometric determination of intracellular DNR content as a discriminant assay for detecting the frequency of drug-resistant cells in a mixed population was acceptable only when the frequency of resistant cells in the population exceeded 10%. In contrast, volume normalization of intracellular DNR content enhanced the ability of the flow-cytometric assay to discriminate resistant cells by 10-fold for P388 cells and 100-fold for HL-60 cells.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
5.
The total protein content and cell size distribution of recombinantSaccharomyces cerevisiae cells were analyzed by flow cytometry. The recombinant strain containing a regulatableSUC2 promoter and the host strain were compared when grown under similar conditions in a batch culture. Recombinant and host cells
maintained similar size and total protein content while cloned-gene expression was repressed by glucose levels greater than
0.2% (w/v). Following derepression, recombinant cells demonstrated a mean total protein content and mean cell size 1.5–2 times
greater than that of the host cells. In addition, these simple flow cytometric measurements of the changes in cell size and
total protein content were found to closely follow diauxic growth ofSaccharomyces cerevisiae in batch culture. 相似文献
6.
Discrepancies between cell volume and organic content in semi-continuous cultures of a marine microalga 总被引:2,自引:0,他引:2
J. Fábregas A. Cid E. Morales B. Cordero A. Otero 《Letters in applied microbiology》1996,22(3):206-208
J. FÁBREGAS, A. CID, E. MORALES, B. CORDERO AND A. OTERO. 1996. Changes in average cell volume, measured by flow cytometry, and cell organic content were studied in light/dark synchronized semi-continuous cultures of the marine microalga Phaeodactylum tricornutum . Cell volume and organic content both increased with nutrient concentration at all the renewal rates tested. Cell volume against renewal rate, at each nutrient concentration, followed a U-shaped curve with smallest cells at intermediate renewal rates. In contrast, cell organic content decreased continuously with increasing renewal rate. The variation in cell volume and organic content, related to culture conditions, should be taken into account if biochemical composition and productivity of microalgal cultures are assessed on the basis of cell counts. 相似文献
7.
Method of flow cytometric analysis have recently been developed that make it possible to obtain segregated data on a single cell basis. In particular, it has been previously demonstrated that protein distributions obtained by flow cytometry give information about the law of growth of the cell population and the law of growth of the single cell; thus these distribution show how the microbial population is actually growing at the moment of the analysis and may yield more accurate and predictive information. We have extended the analysis of protein distribution and cell volume distribution to continuous cultures of Saccharomyces cerevisiae growing in a glucose-limited chemostat. We have found that: (1) to each dilution rate corresponds a given protein and volume distribution that does not change with time in steady state cultures; (2) there is a good proportionality between the average cell volume and the average protein content; (3) the protein distribution obtained can be easily analyzed with the model of growth of yeast previously developed in our laboratory; (4) the analysis of perturbed states shows that both protein distribution and volume distribution change very quickly; thus they are very sensitive parameters and can be used for monitoring and controlling industrial fermentation. 相似文献
8.
The relationship between protein thiols (PSH) and cell proliferation was examined in ethanol-fixed rat hepatocytes. A new protocol was developed for simultaneous measurement of protein thiol vs. DNA content by flow cytometry. The fluorescent dye o-phthalaldehyde (OPT) was used for flow cytometric measurements of protein thiol groups. The influence of nonprotein thiols was examined by monitoring the cell cycle of cells in which the glutathione content (GSH) was modified by treatment with buthionine sulphoximine (BSO). Three rat liver cell lines (IAR 20, IAR 6.1, IAR 6.1RT7) were used: these cell lines possess different growth characteristics and degrees of tumorigenicity, which made it possible to analyse changes in PSH during normal and deranged cell proliferation. The effects on the cell cycle of the changes in PSH due to the depletion of GSH were measured by 5-bromo-2'-deoxyuridine (BrdUrd) incorporation and flow cytometry. The data obtained can be summarised as follows: a) OPT fluorescence increases with increasing DNA content in all rat liver cell lines examined; b) the greatest variation in PSH content occurs in G1. There is a smaller variation in G2 + M, and PSH levels are relatively invariant throughout S-phase; c) a higher content of PSH is found in the tumorigenic cell lines; d) the amount and distribution of PSH is not affected by BSO treatment; e) kinetic studies indicate that BSO treatment has no effect on the ability of the IAR rat liver cell lines to progress through the cycle. 相似文献
9.
J. E. Keys E. Cifrian A. J. Guidry H. M. Farrell 《In vitro cellular & developmental biology. Animal》1997,33(3):206-211
Summary Cellular DNA, milk protein content, and protein secretion by bovine mammary explants were compared to cultures of confluent
and growing primary bovine mammary secretory cells over 4 d. Explants were obtained at slaughter from eight Holstein cows
(120 ± 35 d lactation). Primary cells were grown to confluence, cryopreserved, thawed, and cultured through five passages.
Explants and cells were cocultured with liver and adipose tissue in the presence of somatotropin, insulinlike growth factor-I,
and somatotropin + insulinlike growth factor-I. Cellular DNA and milk proteins were assayed using fluorescent probes and flow
cytometry. Media proteins were assayed by densitometer scanning of electrophoresis gel bands. DNA content of explant, confluent,
and growing primary cells increased similarly through the 96 h incubation. DNA content in G0G1 phase was increased by: (a) insulinlike growth factor-I in explant cells; (b) somatotropin, insulinlike growth factor-I,
and their combination in confluent primary cells; and (c) the combination of somatotropin and insulinlike growth factor in
growing primary cells. Approximately 65% of explant and confluent primary cells were in the G0G1 or differentiated phase compared to 47% for the growing primary cells. Whey protein content and secretion were similar among
cell types. Explant cells contained and secreted more β-casein than primary cells but secretion trends for β-casein and k-casein
were similar after 48 h for both cell types. Results suggest that primary cell cultures are comparable to explant cultures
when used to study mechanisms of DNA and milk protein synthesis and secretion. 相似文献
10.
Teodori L Tagliaferri F Stipa F Valente MG Coletti D Manganelli A Guglielmi M D'Angelo LS Schäfer H Göhde W 《In vitro cellular & developmental biology. Animal》2000,36(3):153-162
Summary In order to isolate, characterize, and establish culture cell lines with different diagnostic and prognostic significance,
derived from multiclonal neoplasms, a ductal infiltrating mammary tumor was induced in rats by 7,12-dimethylbenz[a]anthracene. Clones with different DNA/protein content, being the DI of 1.16, 1.30, and 1.60, respectively, were observed
in the primary tumor. Biparametric flow cytometry suggested that the clone at 1.30 is made up of two subpopulations with different
protein and slightly different DNA contents. The culture, after a few passages, exhibited the presence of aneuploid cells
and the absence of diploid components, demonstrating that only tumor cells survived. The limiting dilution method gave rise
to four lines with DI of 1.16, 1.25, 1.30, and 1.50; a mean chromosome number of 45, 46, 47, and 88, respectively; and different
morphological and ultrastructural features. These characteristics were stable during the experimental procedure, that is,
for about 20 passages. Conversely, the detection of cytoskeletal proteins indicated that the tumor epithelial cells underwent
early dedifferentiation into sarcoma-like cells showing markers of stromal cell type and thus exhibiting phenotypic instability
in vitro, a feature reported in many advanced human breast cancers in vivo. In conclusion, this cellular model represents
the in vivo situation and appears suitable for in vitro studies of tumor cell characteristics and might be used to predict
clinical behavior. 相似文献
11.
Summary Changes in protein content during the cell cyle of microorganisms can be monitored rapidly by flow cytometry.Abbreviations YEP
yeast extract peptone
- FITC
Fluorescein isothiocyanate 相似文献
12.
The relationship between RNA content, cell volume and growth potential in ageing human embryonic mesenchymal cells 总被引:1,自引:0,他引:1
Modal cell volume and cellular RNA content were meaume was found to correlate with both culture replication rate and cellular RNA content. This observation suggests that cell volume changes may be related to the rate of cell division during ageing in vitro, and that both cell volume and RNA content may be closely controlled. There was no change in the relative proportions of the major rRNA species during the cultures' lifespan. 相似文献
13.
Dry weight biomass is an important parameter in algaculture. Direct measurement requires weighing milligram quantities of dried biomass, which is problematic for small volume systems containing few cells, such as laboratory studies and high throughput assays in microwell plates. In these cases indirect methods must be used, inducing measurement artefacts which vary in severity with the cell type and conditions employed. Here, we utilise flow cytometry pulse width data for the estimation of cell density and biomass, using Chlorella vulgaris and Chlamydomonas reinhardtii as model algae and compare it to optical density methods. Measurement of cell concentration by flow cytometry was shown to be more sensitive than optical density at 750 nm (OD750) for monitoring culture growth. However, neither cell concentration nor optical density correlates well to biomass when growth conditions vary. Compared to the growth of C. vulgaris in TAP (tris-acetate-phosphate) medium, cells grown in TAP + glucose displayed a slowed cell division rate and a 2-fold increased dry biomass accumulation compared to growth without glucose. This was accompanied by increased cellular volume. Laser scattering characteristics during flow cytometry were used to estimate cell diameters and it was shown that an empirical but nonlinear relationship could be shown between flow cytometric pulse width and dry weight biomass per cell. This relationship could be linearised by the use of hypertonic conditions (1 M NaCl) to dehydrate the cells, as shown by density gradient centrifugation. Flow cytometry for biomass estimation is easy to perform, sensitive and offers more comprehensive information than optical density measurements. In addition, periodic flow cytometry measurements can be used to calibrate OD750 measurements for both convenience and accuracy. This approach is particularly useful for small samples and where cellular characteristics, especially cell size, are expected to vary during growth. 相似文献
14.
Changes during subclone development and ageing of human antibody-producing recombinant CHO cells. 总被引:3,自引:0,他引:3
K Strutzenberger N Borth R Kunert W Steinfellner H Katinger 《Journal of biotechnology》1999,69(2-3):215-226
Some of the problems encountered with human or human-mouse heterohybridomas, such as low growth rates and high serum requirements, have led to the increased use of recombinant cell lines for production of human antibodies. To evaluate the suitability of such alternative cell lines for the production of human antibodies we have analysed several subclones with differing specific production rates of a recombinant CHO cell line. Gene copy number and site of chromosomal integration for the light and heavy chain and the dhfr gene were determined by in-situ hybridisation. Specific mRNA content was analysed by Northern blot. In addition the intracellular content in light and heavy chain was measured by flow cytometry and the specific secretion rates were determined. The stability of gene expression was followed in the highest producing subclone for over a year. As previously seen in heterohybridoma cells a high expression rate of light chain is beneficial in speeding up secretion rates of whole antibody. When grown in the presence of G418 and methotrexate the amplified gene copies in the genome of recombinant CHO cells were stable over more than 100 passages. However, the expression of light chain, and with it the secretion rate, decreased with time. The low intracellular concentration of light chain resulted in accumulation of heavy chain in the endoplasmic reticulum due to retention by chaperones. The specific secretion rate decreased by 50% after 100 passages. When no G418 or methotrexate were present 75% of the gene copies were lost after 100 passages. 相似文献
15.
The distribution of DNA among bacterioplankton and bacterial isolates was determined by flow cytometry of DAPI (4',6'-diamidino-2-phenylindole)-stained organisms. Conditions were optimized to minimize error from nonspecific staining, AT bias, DNA packing, changes in ionic strength, and differences in cell permeability. The sensitivity was sufficient to characterize the small 1- to 2-Mb-genome organisms in freshwater and seawater, as well as low-DNA cells ("dims"). The dims could be formed from laboratory cultivars; their apparent DNA content was 0.1 Mb and similar to that of many particles in seawater. Preservation with formaldehyde stabilized samples until analysis. Further permeabilization with Triton X-100 facilitated the penetration of stain into stain-resistant lithotrophs. The amount of DNA per cell determined by flow cytometry agreed with mean values obtained from spectrophotometric analyses of cultures. Correction for the DNA AT bias of the stain was made for bacterial isolates with known G+C contents. The number of chromosome copies per cell was determined with pure cultures, which allowed growth rate analyses based on cell cycle theory. The chromosome ratio was empirically related to the rate of growth, and the rate of growth was related to nutrient concentration through specific affinity theory to obtain a probe for nutrient kinetics. The chromosome size of a Marinobacter arcticus isolate was determined to be 3.0 Mb by this method. In a typical seawater sample the distribution of bacterial DNA revealed two major populations based on DNA content that were not necessarily similar to populations determined by using other stains or protocols. A mean value of 2.5 fg of DNA cell(-1) was obtained for a typical seawater sample, and 90% of the population contained more than 1.1 fg of DNA cell(-1). 相似文献
16.
W Giaretti A Di Vinci E Geido B Marsano M Minks S Bruno 《Cell and tissue kinetics》1990,23(5):473-485
Progressive in vitro culturing of interleukin-3 (IL-3) dependent normal murine mastocytes (PB-3) resulted in a variant cell line (PB-1) able to grow without exogenous IL-3 and which was tumorogenic in syngenic mice. Bivariate flow cytometry was used to evaluate the c-myc protein and DNA content of PB-3 and PB-1 cells. The c-myc protein was detected by specific monoclonal antibodies. Kinetic characteristics of PB-3 and PB-1 cell lines, namely, the duration of the G1, S and G2 + M cell cycle phases were also evaluated using the bromodeoxyuridine (BrdU) pulse-chase method and BrdU/DNA flow cytometry. Levels of c-myc protein in PB-1 cells were about two-fold higher than those of PB-3 cells in all cell cycle phases. Mean duration of the cell cycle (Tc) was 15.3 h for PB-3 cells and 12.4 h for PB-1 cells. Shortening in Tc for the transformed cells was due to a decrease of nearly 30% in mean duration of the G1 phase (from 8 h to 5.7 h). No significant differences were found in the duration of the S and G2 + M phases. These results indicate that acquired IL-3 independency in vitro and tumorogenicity of PB-1 cells were accompanied by a doubling of c-myc protein level and by a parallel shortening, or bypass, of the regulatory events within the G1 phase of the cell cycle. 相似文献
17.
Antibodies to tubulin and actin bind to the surface of a human monocytic cell line, U937 总被引:1,自引:0,他引:1
S B Por M A Cooley S N Breit R Penny P W French 《The journal of histochemistry and cytochemistry》1991,39(7):981-985
Intermittent reports of cytoskeleton proteins (actin and tubulin) on the cell surface have appeared over the last 13 years. Whereas most have concentrated on lymphocytes, this study provides evidence for the presence of these proteins on the surface of a human cultured monocyte-like cell line, U937. Both actin and tubulin were detected on the surface of U937 cells by flow cytometry, using an indirect staining procedure based on biotin-streptavidin-phycoerythrin, chosen for greater sensitivity. By use of this procedure, the majority of viable unstimulated U937 cells stained positively for actin and tubulin, although the level of fluorescence intensity was low. With an antibody specific for tyrosine-tubulin, most of the surface tubulin was also found to be tyrosinylated. For vimentin, an intermediate filament protein abundantly present in the cytoplasm of U937 cells, no staining could be detected. Confirmation of the flow cytometry data for surface actin and tubulin on unstimulated U937 cells was achieved by direct vesualization using a confocal laser scanning microscope. When U937 cells were activated with PMA and LPS, a marked reduction in the level of cell surface actin and tubulin occurred. The role of cell surface actin and tubulin on unstimulated U937 cells, in terms of monocyte function, remains to be elucidated. 相似文献
18.
Abstract. Nuclear DNA content was assessed in multidrug-resistant (MDR) cells by image and flow cytometry. Two human MDR cell lines (K562-Dox and CEM-VLB) obtained by in vitro drug selection and overexpressing mdr1 gene were compared to their respective sensitive counterparts (K562 and CCRF-CEM) and to the MDR hamster LR73-R cell line obtained by transfection of mouse mdr1 cDNA. Both cell lines obtained by selection displayed a decreased DNA content, as measured by image cytometry after Feulgen staining, or by flow cytometry after staining with propidium iodide, ethidium bromide, or Hoechst 33342. This decrease was not accompanied by changes in cell cycle phase distribution of cells. Moreover, image cytometry of cells stained after various hydrolysis times in 5 M HCl indicated that MDR cells displayed the same hydrolysis kinetics and sensitivity as drug-sensitive cells with a well-preserved stoichiometry of the Feulgen reaction. LR73-R cells transfected with mdr1 cDNA exhibited only a very limited change in propidium iodide staining as compared with sensitive LR73 cells, suggesting that mdr1 gene overexpression alone could not account for the alterations in DNA content observed in the selected MDR cells. 相似文献
19.
目的:分析体外传代培养至23代,人脐带间充质干细胞(human umbilical cord-MSCs, hUC-MSCs)多向分化能力的改变,以探索hUC-MSCs体外诱导分化的最佳时间窗。方法:采用胶原酶消化法提取hUC-MSCs,并用胰酶消化传代;收集不同代细胞,用流式细胞术检测细胞表面抗原及细胞周期;MTT法检测不同代细胞的增殖活性;取不同代细胞进行成骨、成软骨及成脂肪诱导鉴定;并利用实时定量PCR分别检测OCT-4、SOX-2、Nanog的mRNA表达水平。结果:用胶原酶消化法可获得形态均一,可稳定传代23代以上的hUC-MSCs;体外传代培养至23代,细胞表面标志物表达率无明显改变;细胞生长曲线形态相似;细胞周期亦无明显差异,(73.04±1.15)%的细胞处于G0/G1期;细胞均可被诱导成骨细胞、软骨细胞和脂肪细胞;不同代细胞OCT-4、SOX-2、Nanog 的mRNA表达无显著差异。结论:体外培养至23代,随着传代次数的增加,hUC-MSCs的多向分化能力并未受影响。 相似文献
20.
The development of transformed cell lines evolving from an embryo fibroblastic C3H primary culture was followed before and after the ageing crisis using different techniques. By flow cytometry, alteration of subpopulations having different DNA content and altered metabolic activity was observed after the crisis, with the trend to assume a near tetraploid DNA index at higher passages. The fibrin clot retractile activity was lost in all cases during the ageing crisis, but the outcome did not present uniform values of growth characteristics or chromosome number and tumorigenicity appeared to be a nonstable property of the transformed cell lines. 相似文献