共查询到20条相似文献,搜索用时 46 毫秒
1.
I. Eujayl M. Baum W. Powell W. Erskine E. Pehu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):83-89
A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 recombinant inbred
lines (F6:8) obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average distance of
6.0 cM between adjacent markers. Previously mapped RFLP markers were used as anchor probes. The morphological markers, pod
indehiscence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion.
More than one-fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion
than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was seldom observed. This is the
most extensive genetic linkage map of lentil to-date. The marker density of this map could be used for the identification
of markers linked to quantitative trait loci in this population.
Received: 6 November 1997 / Accepted: 10 February 1998 相似文献
2.
A genetic map of Asparagus officinalis based on integrated RFLP, RAPD and AFLP molecular markers 总被引:6,自引:0,他引:6
A. Spada E. Caporali G. Marziani P. Portaluppi F. M. Restivo F. Tassi A. Falavigna 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1083-1089
An integrated genetic map of the dioecious species Asparagus officinalis L. has been constructed on the basis of RFLP, RAPD, AFLP and isoenzyme markers. The segregation analysis of the polymorphic
markers was carried out on the progeny of five different crosses between male and female doubled-haploid clones generated
by anther culture. A total of 274 markers have been organized to ten linkage groups spanning 721.4 cM. Since the haploid chromosome
number of asparagus is ten, the established linkage groups probably represent the different chromosomes; however, the only
group associated with a specific chromosome is the one which includes sex, whose determinant genes have been located on chromosome
5. A total of 33 molecular markers (13 RFLPs, 18 AFLPs, 2 RAPDs and 1 isoenzyme) have been located on this chromosome. The
closest marker to the sex determinant is the AFLP SV marker at 3.2 cM.
Received: 26 March 1998 / Accepted: 30 April 1998 相似文献
3.
Identification of quantitative trait loci contributing to Fusarium wilt resistance on an AFLP linkage map of flax (Linum usitatissimum) 总被引:3,自引:0,他引:3
W. Spielmeyer A. G. Green D. Bittisnich N. Mendham E. S. Lagudah 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(4):633-641
An AFLP genetic linkage map of flax (Linum usitatissimum) was used to identify two quantitative trait loci (QTLs) on independent linkage groups with a major effect on resistance
to Fusarium wilt, a serious disease caused by the soil pathogen Fusarium oxysporum (lini). The linkage map was constructed using a mapping population from doubled-haploid (DH) lines. The DH lines were derived
from the haploid component of F2 haploid-diploid twin seed originating from a cross between a polyembryonic, low-linolenic-acid genotype (CRZY8/RA91) and
the Australian cultivar ‘Glenelg’. The AFLP technique was employed to generate 213 marker loci covering approximately 1400 cM
of the flax genome (n=15) with an average spacing of 10 cM and comprising 18 linkage groups. Sixty AFLP markers (28%) deviated
significantly (P<0.05) from the expected segregation ratio. The map incorporated RFLP markers tightly linked to flax rust (Melamspora lini) resistance genes and markers detected by disease resistance gene-like sequences. The study illustrates the potential of
the AFLP technique as a robust and rapid method to generate moderately saturated linkage maps, thereby allowing the molecular
analysis of traits, such as resistance to Fusarium wilt, that show oligogenic patterns of inheritance.
Received: 8 December 1997 / Accepted: 7 April 1998 相似文献
4.
L. Gentzbittel E. Mestries S. Mouzeyar F. Mazeyrat S. Badaoui F. Vear D. Tourvieille de Labrouhe P. Nicolas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):218-234
A map of the sunflower genome, based on expressed sequences and consisting of 273 loci, was constructed. The map incorporates
data from seven F2 populations, for a total of 1115 individuals. Two hundred and fourty five loci corresponding to 170 anonymous cDNA markers
and four loci for morphological markers were mapped. We also mapped 18 loci corresponding to previously described genes or
to sequences obtained through homology cloning. The unit maps vary from 774 cM to 1060 cM, with an average value of 14 major
linkage groups. The integrated map is arranged in 17 major linkage groups including 238 loci, plus four small segments with
2–5 marker loci; and covers 1573 cM with an overall average marker interval of 7 cM. Thirty five percent of the markers were
dominant in nature and 30% showed inter-linkage group duplication without any indication of homoeologous linkage groups. Evidence
is provided for the independence of two distinct fertility restoration genes, for the presence of two loosely linked branching
loci, and for marker tightly linked to the Rf1 restoration locus. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses
and marker-assisted selection.
Received: 27 August 1998 / Accepted: 28 December 1998 相似文献
5.
B. S. Vivek P. W. Simon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):58-64
A 109-point linkage map consisting of three phenotypic loci (P
1, Y
2, and Rs), six restriction fragment length polymorphisms (RFLPs), two random amplified polymorphic DNAs (RAPDs), 96 amplified fragment
length polymorphisms (AFLPs), and two selective amplification of microsatellite polymorphic loci (SAMPL) was constructed for
carrot (Daucus carota L. ssp. sativus; 2n=2x=18). The incidence of polymorphism was 36% for RFLP probes, 20% for RAPD primers, and 42% for AFLP primers. The overall incidence
of disturbed segregation was 18%. Linkage relationships at a LOD score of 4.0 and θ=0.25 indicated 11 linkage groups. The
total map length was 534.4 cM and the map was clearly unsaturated with markers spaced at 4.9 cM. AFLP P6B15 was 1.7 cM from
P
1, AFLP P1B34 was 2.2 cM from Y
2, and AFLP P3B30XA was 8.1 cM from Rs.
Received: 2 September 1998 / Accepted: 28 November 1998 相似文献
6.
M. Yaneshita S. Kaneko T. Sasakuma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(5):751-756
A RFLP linkage map of Zoysia spp. (2n=40), a warm-season turfgrass, was constructed by using the self-pollinated progenies obtained from an interspecific
hybrid. Out of 115 DNA clones tested, 100 (87.0%), including 55 genomic clones, 38 cDNA clones, and seven gene clones encoding
photosynthetic enzymes showed allelic-RFLP banding patterns among the parental accessions. We found that 26 probes detected
two or more loci segregating in the self-pollinated progenies independently. The RFLP linkage map of Zoysia spp. consists of 115 loci in 22 linkage groups ranging in size from 12.5 cM to 141.3 cM with a total map distance of 1506 cM.
Six RFLP loci (5.4%) showed significant segregation distortion (P<0.01). Two loci out of six were mapped to linkage group II, and another two loci were mapped to group VII. In the RFLP linkage
map of zoysiagrass, five pairs of linkage groups sharing a series of duplicated loci with approximately the same order were
identified. Therefore, we conclude that Zoysia spp. with 2n=40 should be considered as allotetraploids, which might have evolved from progenitors with a basic chromosome
number of ten (x=10).
Received: 20 March 1998 / Accepted: 17 September 1998 相似文献
7.
A genetic linkage map of cowpea (Vigna unguiculata) developed from a cross between two inbred, domesticated lines 总被引:1,自引:0,他引:1
C. M. Menéndez A. E. Hall P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1210-1217
We have constructed a genetic linkage map within the cultivated gene pool of cowpea (2n=2x=22) from an F8 recombinant inbred population (94 individuals) derived from a cross between the inbreds IT84S-2049 and 524B. These breeding
lines, developed in Nigeria and California, show contrasting reactions against several pests and diseases and differ in several
morphological traits. Parental lines were screened with 332 random RAPD decamers, 74 RFLP probes (bean, cowpea and mung bean
genomic DNA clones), and 17 AFLP primer combinations. RAPD primers were twice as efficient as AFLP primers and RFLP probes
in detecting polymorphisms in this cross. The map consists of 181 loci, comprising 133 RAPDs, 19 RFLPs, 25 AFLPs, three morphological/classical
markers, and a biochemical marker (dehydrin). These markers identified 12 linkage groups spanning 972 cM with an average distance
of 6.4 cM between markers. Linkage groups ranged from 3 to 257 cM in length and included from 2 to 41 markers, respectively.
A gene for earliness was mapped on linkage group 2. Seed weight showed a significant association with a RAPD marker on linkage
group 5. This map should facilitate the identification of markers that “tag” genes for pest and disease resistance and other
traits in the cultivated gene pool of cowpea.
Received: 16 September 1996 / Accepted: 25 April 1997 相似文献
8.
V. Laucou K. Haurogné N. Ellis C. Rameau 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):905-915
A genetic linkage map of Pisum sativum L. was constructed based primarily on RAPD markers that were carefully selected for their reproducibility and scored in a
population of 139 recombinant inbred lines (RILs). The mapping population was derived from a cross between a protein-rich
dry-seed cultivar ‘Térèse’ and an increased branching mutant (K586) obtained from the pea cultivar ‘Torsdag’. The map currently
comprises nine linkage groups with two groups comprising only 6 markers (n=7 in pea) and covers 1139 cM. This RAPD-based map has been aligned with the map based on the (JI281×JI399) RILs population
that currently includes 355 markers in seven linkage groups covering 1881 cM. The difference in map lengths is discussed.
For this alignment 7 RFLPs, 23 RAPD markers, the morphological marker le and the PCR marker corresponding to the gene Uni were used as common markers and scored in both populations.
Received: 13 March 1998 / Accepted: 29 April 1998 相似文献
9.
Molecular genetic linkage maps for allotetraploid Leymus wildryes (Gramineae: Triticeae). 总被引:2,自引:0,他引:2
Xiaolei Wu Steven R Larson Zanmin Hu Antonio J Palazzo Thomas A Jones Richard R-C Wang Kevin B Jensen N Jerry Chatterton 《Génome》2003,46(4):627-646
Molecular genetic maps were constructed for two full-sib populations, TTC1 and TTC2, derived from two Leymus triticoides x Leymus cinereus hybrids and one common Leymus triticoides tester. Informative DNA markers were detected using 21 EcoRI-MseI and 17 PstI-MseI AFLP primer combinations, 36 anchored SSR or STS primer pairs, and 9 anchored RFLP probes. The 164-sib TTC1 map includes 1069 AFLP markers and 38 anchor loci in 14 linkage groups spanning 2001 cM. The 170-sib TTC2 map contains 1002 AFLP markers and 36 anchor loci in 14 linkage groups spanning 2066 cM. Some 488 homologous AFLP loci and 24 anchor markers detected in both populations showed similar map order. Thus, 1583 AFLP markers and 50 anchor loci were mapped into 14 linkage groups, which evidently correspond to the 14 chromosomes of allotetraploid Leymus (2n = 4x = 28). Synteny of two or more anchor markers from each of the seven homoeologous wheat and barley chromosomes was detected for 12 of the 14 Leymus linkage groups. Moreover, two distinct sets of genome-specific STS markers were identified in these allotetraploid Leymus species. These Leymus genetic maps and populations will provide a useful system to evaluate the inheritance of functionally important traits of two divergent perennial grass species. 相似文献
10.
Preliminary genetic linkage maps were constructed for the Pacific abalone (Haliotis discus hannai Ino) using amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA (RAPD), and microsatellite markers
segregating in a F1 family. Nine microsatellite loci, 41 RAPD, and 2688 AFLP markers were genotyped in the parents and 86 progeny of the mapping
family. Among the 2738 markers, 384 (including 365 AFLP markers, 10 RAPD markers, and 9 microsatellite loci) were polymorphic
and segregated in one or both parents: 241 in the female and 146 in the male. The majority of these markers, 232 in the female
and 134 in the male, segregated according to the expected 1:1 Mendelian ratio (α = 0.05). Two genetic linkage maps were constructed
using markers segregating in the female or the male parent. The female framework map consisted of 119 markers in 22 linkage
groups, covering 1773.6 cM with an average intermarker space of 18.3 cM. The male framework map contained 94 markers in 19
linkage groups, spanning 1365.9 cM with an average intermarker space of 18.2 cM. The sex determination locus was mapped to
the male map but not to the female map, suggesting a XY-male determination mechanism. Distorted markers showing excess of
homozygotes were mapped in clusters, probably because of their linkage to a gene that is incompatible between two parental
populations. 相似文献
11.
Genetic linkage map in sour cherry using RFLP markers 总被引:6,自引:0,他引:6
D. Wang R. Karle T. S. Brettin A. F. Iezzoni 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(8):1217-1224
Restriction fragment length polymorphism (RFLP) linkage maps of two tetraploid sour cherry (Prunus cerasus L., 2n=4x=32) cultivars, Rheinische Schattenmorelle (RS) and Erdi Botermo (EB), were constructed from 86 progeny from the cross RS×EB.
The RS linkage map consists of 126 single-dose restriction fragment (SDRF, Wu et al. 1992) markers assigned to 19 linkage
groups covering 461.6 cM. The EB linkage map has 95 SDRF markers assigned to 16 linkage groups covering 279.2 cM. Fifty three
markers mapped in both parents were used as bridges between both maps and 13 sets of homologous linkage groups were identified.
Homoeologous relationships among the sour cherry linkage groups could not be determined because only 15 probes identified
duplicate loci. Fifty nine of the markers on the linkage maps were detected with probes used in other Prunus genetic linkage maps. Four of the sour cherry linkage groups may be homologous with four of the eight genetic linkage groups
identified in peach and almond. Twenty one fragments expected to segregate in a 1 : 1 ratio segregated in a 2 : 1 ratio. Three
of these fragments were used in the final map construction because they all mapped to the same linkage group. Six fragments
exhibited segregation consistent with the expectations of intergenomic pairing and/or recombination.
Received: 1 April 1998 / Accepted: 9 June 1998 相似文献
12.
A genetic linkage map of tef [Eragrostis tef (Zucc.) Trotter] based on amplified fragment length polymorphism 总被引:2,自引:0,他引:2
G. Bai H. Tefera M. Ayele H. T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):599-604
A genetic linkage map of tef was constructed with amplified fragment length polymorphism (AFLP) markers using F5 recombinant inbred lines (RILs) derived by single seed descent from the intraspecific cross of ’Kaye Murri’×’Fesho’. A total
of 192 EcoRI/MseI primer combinations were screened for parental polymorphism. Around three polymorphic fragments per primer combination were
detected, indicating a low polymorphism level in tef. Fifty primer combinations were selected to assay the mapping population,
and 226 loci segregated among 85 F5 RILs. Most AFLP loci behaved as dominant markers (presence or absence of a band), but about 15% of the loci were codominant.
Significant deviations from the expected Mendelian segregation ratio were observed for 26 loci. The genetic linkage map comprised
211 markers assembled into 25 linkage groups and covered 2,149 cM of genome. AFLP is an efficient marker system for mapping
plant species with low polymorphism such as tef. This is the first genetic linkage map constructed for tef. It will facilitate
the mapping of genes controlling agronomically important traits and cultivar improvement in tef.
Received: 27 April 1998 / Accepted: 4 January 1999 相似文献
13.
Vilanova S Romero C Abbott AG Llácer G Badenes ML 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(2):239-247
A genetic linkage map of apricot ( Prunus armeniaca L.) was constructed using AFLP and SSR markers. The map is based on an F(2) population (76 individuals) derived from self-pollination of an F(1) individual ('Lito') originated from a cross between 'Stark Early Orange' and 'Tyrinthos'. This family, designated as 'Lito' x 'Lito', segregated for two important agronomical traits: plum pox virus resistance (PPV) and self-incompatibility. A total of 211 markers (180 AFLPs, 29 SSRs and two agronomic traits) were assigned to 11 linkage groups covering 602 cM of the apricot genome. The average distance (cM/marker) between adjacent markers is 3.84 cM. The PPV resistance trait was mapped on linkage group G1 and the self-incompatibility trait was mapped on linkage group G6. Twenty two loci held in common with other Prunus maps allowed us to compare and establish homologies among the respective linkage groups. 相似文献
14.
M. M. Messmer M. Keller S. Zanetti B. Keller 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1163-1170
We constructed a genetic map of a cross between the Swiss winter wheat (Triticum aestivum L.) variety Forno and the Swiss winter spelt (Triticum spelta L.) variety Oberkulmer. For the linkage analysis,176 polymorphic RFLP probes and nine microsatellites were tested on 204
F5 recombinant inbred lines (RILs) of Forno×Oberkulmer revealing 242 segregating marker loci. Thirty five percent of these loci
showed significant (P>0.05) deviation from a 1 : 1 segregation, and the percentage of Forno alleles ranged from 21% to 83% for individual marker
loci. Linkage analysis was performed with the program MAPMAKER using the Haldane mapping function. Using a LOD threshold of
10, we obtained 37 linkage groups. After finding the best order of marker loci within linkage groups by multi-point analysis
we assembled the linkage groups into 23 larger units by lowering the LOD threshold. All except one of the 23 new linkage groups
could be assigned to physical chromosomes or chromosome arms according to hybridisation patterns of nulli-tetrasomic lines
of Chinese Spring and published wheat maps. This resulted in a genetic map comprising 230 marker loci and spanning 2469 cM.
Since the analysed population is segregating for a wide range of agronomically important traits, this genetic map is an ideal
basis for the identification of quantitative trait loci (QTLs) for these traits.
Received: 3 August 1998 / Accepted: 28 November 1998 相似文献
15.
玉米相对饱和遗传连锁图谱构建与一种新的AFLPs共显性分析方法探讨 总被引:5,自引:0,他引:5
利用一个F2作图群体(X178×B73),首先构建了一个含有130个SSRs的玉米连锁框架图,然后用119个AFLPs位点增加图谱密度,得到一个全长1659·3cM,标记间平均间距6·66cM的玉米相对饱和连锁图。同时,对SSRs和AFLPs的一些遗传特性进行了分析,探讨了AFLP标记进行共显性分析的一种新方法。分析表明SSRs和AFLPs分子标记具有多态性和可靠性高等特点,是构建高密度分子标记遗传连锁图的有效技术。加密的玉米遗传连锁图谱为比较基因组研究、数量性状位点(quantitativetraitloci,QTLs)克隆、杂种优势机理研究以及标记辅助选择等提供了技术基础。 相似文献
16.
Zhigang Wei Kaixuan Zhang Chuanping Yang Guifeng Liu Guanjun Liu Lian Lian Hanguo Zhang 《Plant Molecular Biology Reporter》2010,28(1):169-175
Two separate genetic linkage maps for Chinese silver birch based on inter-simple sequence repeat (ISSR) and amplified fragment-length
polymorphism (AFLP) were constructed by a pseudo-testcross mapping strategy. Eighty F1 progenies were obtained from the cross between two parental trees with desirable traits (the paternal one selected from ‘Qinghai’
and the maternal one from ‘Wangqing’). A total of 46 ISSR primers and 31 AFLP primers were employed to generate 102 ISSR and
355 AFLP polymorphic markers in the F1 progenies. About 5.7% of all the markers displayed high segregation distortion with a P value below 0.01 and such markers were not used for map constructions. The paternal map consisted of 137 loci, spread over
13 groups and spanned 694.2 cM at an average distance of 5.1 cM between the markers, while in the maternal map, 147 loci were
distributed in 14 groups covering a map distance about 949.62 cM at an average distance of 6.5 cM. These initial maps can
serve as the basis for developing a more detailed genetic map. 相似文献
17.
Garcia AA Kido EA Meza AN Souza HM Pinto LR Pastina MM Leite CS Silva JA Ulian EC Figueira A Souza AP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(2):298-314
Sugarcane (Saccharum spp.) is a clonally propagated outcrossing polyploid crop of great importance in tropical agriculture. Up to now, all sugarcane
genetic maps had been developed using either full-sib progenies derived from interspecific crosses or from selfing, both approaches
not directly adopted in conventional breeding. We have developed a single integrated genetic map using a population derived
from a cross between two pre-commercial cultivars (‘SP80-180’ × ‘SP80-4966’) using a novel approach based on the simultaneous
maximum-likelihood estimation of linkage and linkage phases method specially designed for outcrossing species. From a total
of 1,118 single-dose markers (RFLP, SSR and AFLP) identified, 39% derived from a testcross configuration between the parents
segregating in a 1:1 fashion, while 61% segregated 3:1, representing heterozygous markers in both parents with the same genotypes.
The markers segregating 3:1 were used to establish linkage between the testcross markers. The final map comprised of 357 linked
markers, including 57 RFLPs, 64 SSRs and 236 AFLPs that were assigned to 131 co-segregation groups, considering a LOD score
of 5, and a recombination fraction of 37.5 cM with map distances estimated by Kosambi function. The co-segregation groups
represented a total map length of 2,602.4 cM, with a marker density of 7.3 cM. When the same data were analyzed using JoinMap
software, only 217 linked markers were assigned to 98 co-segregation groups, spanning 1,340 cM, with a marker density of 6.2 cM.
The maximum-likelihood approach reduced the number of unlinked markers to 761 (68.0%), compared to 901 (80.5%) using JoinMap.
All the co-segregation groups obtained using JoinMap were present in the map constructed based on the maximum-likelihood method.
Differences on the marker order within the co-segregation groups were observed between the two maps. Based on RFLP and SSR
markers, 42 of the 131 co-segregation groups were assembled into 12 putative homology groups. Overall, the simultaneous maximum-likelihood
estimation of linkage and linkage phases was more efficient than the method used by JoinMap to generate an integrated genetic
map of sugarcane.
E.A. Kido, A.N. Meza and H.M.B. Souza contributed equally to this work. 相似文献
18.
F. Perez A. Menendez P. Dehal C. F. Quiros 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1183-1193
A genetic map was constructed from an F2 population of 76 individuals for the purpose of comparing the arrangement of loci in the A and E Solanum genomes. This progeny was derived from an interspecific cross between the species Solanum palustre×Solanum etuberosum, both of which are E-genome species. Two hundred and eighty one probes previously mapped in tomato and potato (A-genome,
as postulated for diploid cultivated potato species by Matsubayashi 1991) disclosed 109 segregating loci in this population.
Of these, 80 loci were linked in 19 linkage groups covering a total of 720.4 cM, with an average of 9 cM between markers.
Although the genetic map of the E-genome showed conservation for most linkage groups with those of tomato and the A-genome,
various translocations and possible inversions and transpositions were detected. It is evident that the accumulation of these
structural changes in the E-genome is sufficient to cause the observed hybrid sterility. The major rearrangements in the E-genome
included multiple translocations involving mosly linkage groups 2 and 8. Also a transposition was detected on group 9, with
the same group-10 inversion distinguishing potato from tomato. Definitively groups 2, 8, 9 and 10, and possibly groups 1,
4 and 12, in the E-genome are structurally different from their homologues in the A-genome. In general, recombination values
were larger in the E- than in the A-genome. The extensive structural differentiation of the E-genome with respect to that
of potato and tomato justifies its present designation as a different genome, which is supported by previous chromosome-pairing
studies. The difficult introgression of desirable traits from the Etuberosum species into potato can be explained by these structural differences.
Received: 1 February 1998 / Accepted: 8 October 1998 相似文献
19.
Ulloa M Meredith WR Shappley ZW Kahler AL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):200-208
An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton (Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from
199 bulk-sampled plots of an F2.3 (HQ95–6×’MD51ne’) population. The map comprises 83 loci mapped to 24 linkage groups with an average distance between markers
of 10.0 centiMorgan (cM), covering 830.1 cM or approximately 18% of the genome. The fourth genetic map was developed from
155 bulk-sampled plots of an F2.3 (119– 5 sub-okra×’MD51ne’) population. This map comprises 56 loci mapped to 16 linkage groups with an average distance between
markers of 9.3 cM, covering 520.4 cM or approximately 11% of the cotton genome. A core of 104 cDNA probes was shared between
populations, yielding 111 RFLP loci. The constructed genetic linkage joinmap from the above four populations comprises 284
loci mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1,502.6 cM or approximately
31% of the total recombinational length of the cotton genome. The linkage groups contained from 2 to 54 loci each and ranged
in distance from 1.0 to 142.6 cM. The joinmap provided further knowledge of competitive chromosome arrangement, parental relationships,
gene order, and increased the potential to map genes for the improvement of the cotton crop. This is the first genetic linkage
joinmap assembled in G. hirsutum with a core of RFLP markers assayed on different genetic backgrounds of cotton populations (Acala, Delta, and Texas plain).
Research is ongoing for the identification of quantitative trait loci for agronomic, physiological and fiber quality traits
on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors (the A and D genomes).
Received: 23 February 2001 / Accepted: 8 June 2001 相似文献
20.
For silver carp (Hypophthalmichthys molitrix), a combined microsatellite (or simple sequence repeat) and amplified fragment length polymorphism (AFLP) sex average linkage map was constructed. A total of 483 markers (245 microsatellites and 238 AFLPs) were assigned to 33 linkage groups. The map spanned 1352.2 cM, covering 86.4% of the estimated genome size of silver carp. The maximum and average spaces between 420 loci were 21.5 cM and 3.2 cM, respectively. The length of linkage groups ranged from 3.6 cM to 98.5 cM with an average of 41.0 cM. The number of markers per group varied from 2 to 44 with an average of 14.6. The AFLP markers significantly improved the integrity of microsatellite-based linkage groups and increased the genome coverage and marker evenness. A genome-wide recombination suppression was observed in male. In an extreme case, six microsatellites co-segregated in male, but spanned a 45.1 cM region in female. 相似文献