The freshwater snail Bulinus tropicus (Planorbidae) in Namibia, characterised according to chromosome number, enzymes and morphology

Freshwater snails collected in central Namibia, south-western Africa, from 15 populations belonging to the Bulinus truncatus/tropicus complex (Planorbidae) are characterised in respect of their chromosome number, morphology, egg proteins and enzymes. The population samples were all consistently diploid and euphallic. The findings are compared with observations on this group of snails in other areas of Africa. It is concluded that the Namibian populations belong to a single species, B. tropicus (Krauss, 1848), of which B. parietalis (Mousson, 1887) is probably a synonym. No evidence was found of any occurrence of the tetraploid species B. truncatus or of snails belonging to the B. africanus group; lack of a potential intermediate host therefore precludes transmission of Schistosoma haematobium in this area.External Scientific Staff of the Medical Research Council of the UK     

A molecular phylogenetic analysis of Bulinus (Gastropoda: Planorbidae) with conserved nuclear genes

Jørgensen, A., Madsen, H., Nalugwa, A., Nyakaana, S., Rollinson, D., Stothard, J. R. & Kristensen, T. K. A molecular phylogenetic analysis of Bulinus (Gastropoda: Planorbidae) with conserved nuclear genes. —Zoologica Scripta, 40, 126–136. Mutational saturation of inspected DNA loci and topological incongruence in the phylogenetic inferences have previously confounded attempts to resolve the evolutionary relationships within the freshwater snail genus Bulinus. Traditionally, the 37 species of Bulinus are placed within the four species groups and the evolutionary divergence between groups is substantial. With an intention to shed new light on species group relationships, the present study was designed to investigate the basal divergences in the phylogeny of Bulinus using highly conserved nuclear genes. The resolved phylogeny inferred that the four species groups of Bulinus were monophyletic and Shimodaira‐Hasegawa topology tests found them to be significantly supported. The Bulinus truncatus/tropicus species complex and Bulinus wrighti (Bulinus reticulatus group) formed a well‐supported sister‐group relationship. The Bulinus africanus species group was the sister‐group to the clade (Bulinus truncatus/tropicus + B. wrighti) with the Bulinus forskalii species group as the sister‐group to these taxa. The sister‐group relationship between Indoplanorbis and Bulinus was non‐significant and the basal clade support of Bulinus improved upon exclusion of Indoplanorbis. The finding of basal long branches of Bulinus species originating from Madagascar strongly suggests the presence of additional cryptic species and an evolutionary scenario influenced by this island’s geological vicariance from the African mainland. Speciation by polyploidy was inferred to have evolved within a clade in the Bulinus truncatus/tropicus species complex. Although the monophyletic status of each species group was firmly supported, it was difficult to establish species group concepts equally across the variations and place this precisely in a specific temporal framework.     

Potential environmental impacts associated with the proposed abstraction of water from the Okavango River in Namibia

The Namibian Department of Water Affairs has in the past faced considerable pressure to relieve the water shortages caused by recent droughts. One of the options considered in 1996, following poor runoff during the 1994/95 and 1995/96 seasons, was a proposal to abstract some 17Mm³ of water per year from the Okavango River at Rundu, and transfer this via a 260km long pipeline to the head of the Eastern National Water Carrier at the town of Grootfontein. Part of the overall evaluation of this scheme included an assessment of the potential environmental impacts that could arise. An initial environmental evaluation was conducted from a point approximately 40 kilometres upstream of Rundu in Namibia, to the distal end of the Okavango Delta at Maun in Botswana.

Hydrological studies showed that the proposed abstraction represented a reduction of approximately 0.32% in the mean annual flow of the Okavango River at Rundu. The abstraction represents 0.17% of the mean annual flow at Mukwe, downstream of the Cuito River confluence. The adverse effects of the proposed water abstraction scheme would be extremely small along the Okavango River in Namibia, whilst outflows from the lower end of the Okavango Delta to the Thamalakane River would be reduced by some 1.44Mm³/year (11%). Additional studies showed that these effects could be reduced by some 10–13% if abstraction was confined to the falling limb of the hydrograph.

Hydrological simulations have shown that the maximum likely loss of inundated area in the Okavango Delta would total approximately 7km² out of some 8 000km². This potential loss in inundated area would be concentrated in the lower reaches of the seasonal swamps and seasonally inundated grasslands, specifically in the lower reaches of the Boro, Gomoti, Santantadibe and Thaoge channels. However, these effects would most likely be expressed as a shoreline effect, with the loss in area spread out over the shoreline and periphery of islands and would not be restricted to a single specific area. This anticipated loss in inundated area is unlikely to have measurable impacts on environmental components.

Overall, the study found no 'fatal flaws' which would prevent the water abstraction scheme from proceeding and the anticipated effects on the Okavango system are more likely to be seen in the Okavango Delta, rather than along the Okavango River. The anticipated ecological implications of the scheme were small in spatial extent and are unlikely to be perceptible against the natural year-to-year variability in inundation of the Okavango Delta or outflows to the Thamalakane River. However, the public perceptions of the proposed water transfer project were strongly negative and appeared to be at least in part due to the very low water levels in the Okavango River and Okavango Delta during the past three years and during the study period. These low water levels were amongst the lowest on record and it is likely that the public would attribute any adverse effect recorded in the future to the abstraction scheme, whether this were true or not. These negative perceptions of the desirability and acceptability of the proposed scheme were strongly linked to potential adverse affects on the tourism industry along the Okavango River and in the Okavango Delta, with possible adverse economic effects on local residents.     

Ecology of freshwater snails in south-western Nigeria. I: Distribution and habitat preferences

Dry and rainy season investigations of diverse freshwater habitats in south-western Nigeria revealed fourteen species of snail comprised of nine pulmonates: Biomphalaria pfeifferi Krauss, Bulinus globosus Morelet, Bulinus rohlfsi Clessin, Lymnaea natalensis Krauss, Physa ( Aplexa) waterloti Germain, Bulinus forskali Ehrenberg, Gyraulus costulatus Krauss, Ferrissia sp, Segmentorbis sp. and five prosobranchs namely, Lanistes libycus Morelet, Lanistes ovum Peters, Pila wernei Philippi, Potadoma moerchi Reeve and Melanoides tuberculata Müller.The influence of diverse chemical and physical properties of water on their occurrence as well as interspecific and snail-plant relationships are discussed and maps showing the distribution of the species encountered are presented. Among the intermediate hosts of Schistosoma, B. globosus was widely distributed and common, B. pfeieri was also widely distributed but infrequent and B. rohlfsi was rare.     

Response of riparian plant communities to distance from surface water in the Okavango Delta,Botswana

The objective of this study was to determine the influence of distance from surface water on riparian woodland communities in the Okavango Delta. Vegetation sampling was conducted in seven sites within the Okavango Delta in 20 m × 10 m belted plots placed perpendicular to the river bank. The plots were placed at 0–10 m, 10–20 m, 20–30 m, 30–40 m and 40–50 m distance classes increasing away from the river bank. Tree height, basal area, species richness, canopy cover and diversity were determined for each distance class. Indicator species analysis was used to determine the characteristic species at each distance class. Single‐factor ANOVA and Tukey post hoc analysis were used to compare species diversity, mean tree height, cover and basal area between distance classes. Correlation between distance from surface water and vegetation parameters was sought using Spearman regression analysis. All parameters except for species richness varied significantly (P < 0.05) along distance from surface water. Distance from surface water was positively correlated all vegetation parameters except for mean species richness/plot. These results show that distance from surface water influences riparian plant community composition and distribution in the Okavango Delta. This implies that riparian plant species can be indicators of long‐term hydrologic conditions in the Delta.     

MANAGEMENT,CONSERVATION AND RESEARCH OF INTERNATIONALLY SHARED WATERCOURSES IN SOUTIIERN AFRICA—NAMIBIAN EXPERIENCE WITH THE OKAVANGO RIVER AND RIVERS OF THE EASTERN CAPRIVI

Summary

Although the emphasis of the recent National Rivers Research Initiative (now the Research Programme for the Management of Rivers) in South Africa was on river research in that country, the reality is that in southern Africa many watercourses are shared by more than one country. Therefore a central issue in integrated river basin management and development should be the recognition of the principle of international joint planning, conservation, monitoring and research.

Co-operation and a better understanding of shared waters would prevent deleterious impacts, not only in the country where they occur, but also in those downstream. With this in mind, since the early 1980s Namibia has worked on joint cross-border projects in efforts to manage and conserve her shared water bodies. These include joint river gauging exercises, biological control of Saivinia molesta in the eastern Caprivi region and, more recently, the application of a biological monitoring technique, the South African Scoring System version 4, to north-eastern perennial rivers.

The formation of the Joint Permanent Water Commission between Botswana and Namibia enabled the Departments of Water Affairs of both countries to work together on their shared rivers. The establishment of the Permanent Okavango River Basin Water Commission between Angola, Botswana and Namibia in 1994 improved this co-operation even further. Namibia and its' counterparts are thus committed to sharing information, co-ordinating research and monitoring activities and to undertaking detailed studies to improve their knowledge of these shared systems.

Such cross-border co-operation is vital for the survival of shared river systems not only as ecological entities in their own right, but also as sources of water, power and the other resources they provide, both directly and indirectly, to the human and wildlife populations that they support.     

The World Crane Action Plan: implications for conservation of cranes and wetlands in Africa

Beilfuss, R.D., Meine, C.D., & Archibald, F.W. 2000. The World Crane Action Plan: implications for conservation of cranes and wetlands in Africa. Ostrich 71 (1 & 2): 227.

The IUCN Species Survival Commission recently published The Cranes: Status Survey and Conservation Action Plan. Prepared over the past three years by the IUCN Crane Specialist Group, the document reviews the status of the world's cranes (Family: Gruidae) and their habitats, summarises recent crane conservation actions, and specifies priority measures for the future. Eleven of the 15 crane species are now proposed for inclusion on the IUCN Red List of Threatened Animals, and several subspecies and populations are critically endangered. Africa supports six species of cranes in 39 countries. The Black Crowned Crane Balearica pavonina, national bird of Nigeria, is resident in western and central Africa. The Grey Crowned Crane B. regulorum, national bird of Uganda, spans eastern and southern Africa. The Wattled Crane Bugeranus carunculahrs, largest and. rarest of Africa's cranes, ranges from Ethiopia to South Africa. The Blue Crane Anthropoides paradiseus, national bird of South Africa, is endemic to South Africa and Namibia. The Demoiselle Crane A. Virgo and Eurasian Crane Grus grus are winter visitors. Cranes have been the focus of intensive captive propagation and reintroduction programs as well as extensive habitat protection efforts. They are also excellent umbrella species for ecosystem-level conservation efforts. Wetlands that provide a home for cranes include the floodplains of the great river systems of Africa, such as Kafue Flats and Zambezi Delta of the Zambezi River system, the Okavango River Delta, the Sudd of the Upper Nile River, the Bangweulu Basin and swamps of the Zaire River system, the Senegal River Delta, and the Inner Niger River Delta, and the wetlands of Lake Chad. Small, isolated wetlands provide vital breeding habitat. This presentation will provide an overview of the action plan for those interested in cranes in particular, as well as those working in ecosystems and regions where cranes occur.     

Aquatic snails of the Bulinus africanus group in Zambia identified according to morphometry and enzymes

The Bulinus africanus species group (Planorbidae) of freshwater snails has been reported to be represented in Zambia by two species, B. africanus (Krauss) and B. globosus (Morelet), both named as intermediate hosts for Schistosoma haematobium. Uncertainty in identification of these snails from morphology led to the present investigation, combining morphometry (shell and copulatory organ) with enzyme analysis. Observations of both kinds were made usually on the same individual snails, from collecting sites mostly in the Lusaka area or at Lake Kariba. Particular attention was given to the proportional relationship between the penis sheath and the preputium of the copulatory organ, a character used previously to distinguish B. africanus from B. globosus in south-eastern Africa. The enzyme profile MDH-1, AcP-2, PGD-1 and PGM-2 was common to all snails examined from 25 populations; GPI and HBDH were polymorphic. The enzyme data indicate that the samples represent a single species. Shell characters varied continuously. The copulatory organ was generally of the form known for B. globosus. Although the copulatory organ of a few individuals had proportions overlapping the range reported for B. africanus, the present variation was continuous and was not bimodal. It is concluded that all these specimens are conspecific and may be identified as B. globosus. Previous identifications of B. africanus from Zambia appear to need substantiation and it seems that if this species is present at all in the sampled areas, it must be uncommon. It is relevant in regard to possible strain differences within S. haematobium in Zambia, that our observations indicate that only a single species of intermediate host is involved in transmission.     

Integrative taxonomy reveals hidden diversity in the southern African darters genus Nannocharax Günther 1867 (Characiformes: Distichodontidae)

The present study explored the diversity of Nannocharax within southern Africa by implementing three species delimitation methods for a data set consisting of 37 mitochondrial cytochrome oxidase subunit I sequences. Two unilocus coalescent methods, the General Mixed Yule Coalescent (GMYC) and the Bayesian implementation of the Poisson Tree Processes (bPTP), and a genetic distance method, the Automatic Barcode Gap Discovery (ABGD), were applied. Both GMYC and bPTP delimited the same operational taxonomic units (OTUs), revealing a higher diversity for the genus in the region than previously recognised, whereas the ABGD failed to delimit the same candidate species. All methods delimited two species groups, and these are supported based on colouration patterning and morphology; the Nannocharax multifasciatus and the Nannocharax macropterus species groups and the delimited OTUs were assigned to each. Two putative new species were identified, Nannocharax cf. lineostriatus “Okavango” from the Okavango River in Angola and N. cf. lineostriatus “Kwanza” from the Kwanza River system in Angola. The distribution of Nannocharax dageti was confirmed for the Upper Zambezi and extended to the Okavango system, and an identification key for the southern Africa Nannocharax species is provided.     

Role of Brycinus lateralis (Teleostei: Alestidae) in dispersal and germination of Nymphaea nouchali (Angiospermae: Nymphaeaceae) seeds on a seasonal floodplain of the Okavango Delta,Botswana

Seed passage through the gut of vertebrates can be important for seed dispersal, but might influence seed viability. The ability of seeds to germinate after ingestion by seed-eating fish is important for the population dynamics of some plant species, and significant in the evolution of plant–fish interactions. Certain fish in the Okavango Delta, Botswana, are fruit- and seed-eaters and could act as seed dispersers. We sampled 14 fish species in 2013, finding Nymphaea nouchali var. caerulea seeds in the digestive tracts of eight, most commonly in the striped robber Brycinus lateralis. Seeds extracted from the gut of this species had an overall mean germination success of 11.7%. This fish species might well be a legitimate seed disperser, having a positive effect on seed dispersal from parent plants in the Okavango Delta. The current study represents one of the first investigations of the likelihood of seed dispersal by fish on the African continent.     

Species-specific primers discriminate schistosome intermediate hosts: unambiguous PCR diagnosis of Bulinus forskalii group taxa (Gastropoda: Planorbidae)

The morphological definition of taxa has proved difficult within the Bulinus forskalii group, which includes intermediate hosts of medically important Schistosoma species in West Africa. Although B. forskalii and B. senegalensis transmit different schistosome species they are conchologically similar and their distributions overlap. Randomly amplified polymorphic DNA (RAPD) allows differentiation of sibling species in the genus Bulinus, but RAPDs are difficult to standardize, impairing their value as a taxonomic tool. Hence, RAPD products diagnostic for either B. senegalensis or B. forskalii from West Africa were cloned, sequenced and a panel of species-specific primers designed. Sequencing of RAPD products identified a homology in two apparently independent RAPD loci, a problem where RAPDs are indiscriminately scored for phylogenetic analyses. Specificity of primers was confirmed by widespread sampling throughout each species' range. This approach produced a simple, robust, unambiguous PCR-based species identification strategy for this difficult group.     

Marcusenius desertus sp. nov. (Teleostei: Mormyridae), a mormyrid fish from the Namib desert

We critically compared Marcusenius specimens from the mouth of the Cunene River on the Namibia/Angola border, a harsh desert environment on the Atlantic Ocean coast virtually devoid of aerial insects with aquatic larvae which are an important food item, with Marcusenius multisquamatus Kramer & Wink, 2013 from the escarpment region of that same river, in a relatively rich and productive subtropical savannah environment. River mouth specimens were differentiated in morphology and electric organ discharges, as determined by ANOVA/MANOVA comparisons, principal component and discriminant analyses on morphological and electrophysiological characters, and genetics, including sequences of the mitochondrial cytochrome b gene, indicating reproductive isolation. Specimens from the river mouth differed from M. multisquamatus, their closest relatives, by having a shorter snout, a smaller eye diameter, and smaller nares separation. River mouth specimens were also differentiated from other, increasingly less-close relatives, such as M. altisambesi Kramer et al., 2007 from the Okavango River, Botswana, and from M. krameri Maake et al., 2014 from the Limpopo System, South Africa. We therefore designate the new species Marcusenius desertus sp. nov. for the Cunene River mouth population.     

Distribution and habitats of Bulinus natalensis and its role as intermediate host of economically important helminth parasites in South Africa

This article focusses on the geographical distribution and habitats of Bulinus natalensis, as reflected by the samples taken from 244 collection sites on record in the database of the National Freshwater Snail Collection (NFSC) at the Potchefstroom campus of the North-West University. This snail species is experimentally susceptible to Schistosoma haematobium and is incriminated in the natural transmission of S. margrebowiei, S. bovis and Calicophoron microbothrium elsewhere in Africa. The 78 different loci (1/16 degree squares) on record reflect a geographical distribution that is largely limited to the KwaZulu-Natal Province. Details of each habitat as described by collectors during surveys, as well as altitude and mean annual air temperature and rainfall for each locality, were processed and chi-squared and effect size values calculated. A decision tree constructed from all the available data indicated that temperature and altitude, followed by the type of water body, seemed to be the more important factors that significantly influenced the distribution of this species in South Africa. The possible role of this species as intermediate host of economically important helminth species is briefly examined. The need to clarify its relationships with B. tropicus and B. depressus, and to update the geographical distribution in South Africa, is also emphasised.     

Experimental taxonomy of Bulinus (Gastropoda: Planorbidae): the West and North African species reconsidered, based upon an electrophoretic study of several enzymes per individual

Horizontal starch gel electrophoresis was used to score mobilities of seven different enzymes in species of Bulinus from North and West Africa. An account of the intra- and inter-population variation observed was given. Based on the data obtained it was suggested that enzymic data should be used as taxonomic characters in parallel with morphological and anatomical characters. It is suggested that enzymic characters could be used to place taxa into synonymy if the data are collected in such a way that one can write up an enzyme profile for the single individual. The nine taxa of Bulinus known to occur in North and West Africa were revised, the taxon B. jousseaumei was synonymized with B. globosus , and the two taxa B. guernei and B. rohlfsi were synonymized with B. truncatus. As a result of the revision only six species could be recognized as valid from the area, these being B. forskalii, B. globosus, B. senegalensis, B. truncatus, B. ugandae and B. umbilicatus. Finally, some enzymic characters are suggested that may be used for a reliable identification of these morphologically very difficult taxa.     

BULINUS AFRICANUS-GROUP SPECIES IN WEST AFRICA DIFFERENTIATED BY MORPHOMETRIC ANALYSIS (GASTROPODA, PLANORBIDAE)

In the present study 241 shells from snails belonging to theBulinus africanus-group in West Africa, were examined in a morphometricanalysis including ten characters. Principal component analysisand stepwise discriminant function analysis were used for analysisof the data. It was shown that Bulinus umbilicatus, B. jousseaumeiand B. globosus are clearly distinct on morphometric shell characters.Besides a general differentiation between the three species,the characters necessary for differentiation between them twoby two are found. The knowledge of the geographic distributionof B. umbilicatus and B. jousseaumei is significantly improved. (Received 18 March 1988; accepted 4 May 1988)     

Predation on snails by an indigenous fish,Sargochromis codringtonii,in ponds in Zimbabwe

The extent to which Sargochromis codringtonii (a known predator of snails) can control snail numbers was investigated in cementlined ponds in order further to evaluate the fish's potential as an agent for the biological control of fresh water snails, especially Bulinus globosus which is the intermediate host for Schistosoma haematobium in Zimbabwe. Bulinus tropicus of all sizes were vulnerable to predation by fish, but both Melanoides tuberculata and Bulinus globosus with shell heights greater than 10mm seemed to be less so. Surprisingly, the density of B. globosus was lower in ponds without fish than in ponds with fish, while the density of B. tropicus was very high in ponds without fish. It was not possible to conclude whether B. globosus benefited by the presence of fish, or was less palatable to the fish than B. tropicus, or whether the high density of B. tropicus in ponds without fish exerted competitive effects on B. globosus. Consequently, further experiments are required to determine whether S. codringtonii can reduce populations of B. globosus in the absence of B. tropicus.     

The effect of temperature,darkness, starvation and various food types on growth,survival and reproduction of Helisoma duryi,Biomphalaria alexandrina and Bulinus truncatus (Gastropoda: Planorbidae)

Helisoma duryi has been proposed as a biological control agent in schistosomiasis due to its superiority in laboratory competition experiments with various species of the intermediate host snails. Therefore it was considered important to evaluate the response of this snail species and the intermediate host species, Biomphalaria alexandrina and Bulinus truncatus, to various physical, chemical and biological factors under laboratory conditions in order to obtain information on the similarities in the ecological niches of these species. The factors considered in the present paper are: temperature, darkness, starvation and food. All three species had optimal growth and egg laying at 26–28 °C. Only H. duryi survived for a longer period at 33°C and it was capable of starting egg laying at this temperature although the onset was delayed. However, low temperature (18°C) caused a relatively larger decrease in egg laying of H. duryi than in the other two species. Growth and egg laying was reduced for H. duryi and B. truncatus kept under darkness and B. alexandrina could not tolerate maintenance under darkness. A few days of starvation of juvenile snails had no effect on later growth and egg laying capacity of the survivors, although mortality in B. truncatus was increased. B. alexandrina had a lower tolerance to starvation than the other two species. Egg laying of snails fed only one of the three laboratory food types decreased for all three species in the order: Vov-vov (dog food in dry pellets), Tetramin (fish food) and lettuce. Combinations of lettuce and one or more proteinaceous food types gave optimal growth and egg laying for all three species.     

INBREEDING DEPRESSION,NEUTRAL POLYMORPHISM,AND COPULATORY BEHAVIOR IN FRESHWATER SNAILS: A SELF-FERTILIZATION SYNDROME

This paper examines the consequences of self-fertilization on life-history traits and neutral genetic polymorphism in natural populations of three species of hermaphrodite freshwater snails: Biomphalaria straminea, Bulinus globosus, and the aphallic species Bulinus truncatus. Life-history traits (fecundity, growth, hatching rate, and survival of offspring) are compared under laboratory conditions between isolated (obligatory selfing) and paired (outcrossing possible) snails in one population of B. straminea and B. globosus and two populations of B. truncatus. The genetic polymorphism of the same four populations is analyzed using electrophoretic markers in B. straminea and B. globosus and microsatellite markers in B. truncatus. In B. truncatus and B. straminea, isolated snails have a higher fecundity than paired snails, whereas the contrary is observed in B. globosus. For all populations, no difference in hatching rate and offspring survival is detected between the two treatments. Genetic analyses using microsatellite markers conducted in B. truncatus on progeny of paired snails reveal a high selfing rate in spite of high copulation rates, highlighting the difficulties of obtaining outcrossing in highly selfing snails. The high survival of selfed offspring in B. truncatus and B. straminea indicates that inbreeding depression is limited. The extent of inbreeding depression in B. globosus is less clear. Overall, fitness decrease in this species is limited to fecundity. The extent of allozyme polymorphism is very limited whereas a much higher variability is observed with microsatellites. Biomphalaria straminea and B. truncatus populations are also characterized by very low observed heterozygosities and large heterozygote deficiencies, whereas the B. globosus population does not exhibit such a deficiency. Overall these results allow the definition of a self-fertilization syndrome in hermaphrodite freshwater snails: selfing populations (such as those of B. straminea and B. truncatus studied here) are characterized by high selfing rates in spite of copulations, limited deleterious effects of selfing, limited neutral genetic polymorphism, and large heterozygote deficiencies.     

Heterogeneity in shore flies – the case of Glenanthe Haliday (Diptera: Ephydridae) in the Old World

Old World species of the genus Glenanthe are treated comprehensively with an emphasis on the unusually diverse morphological heterogeneity discovered in structures of the male terminalia. As perspective for this treatment, the tribe Lipochaetini, in which Glenanthe is placed, is characterized and discussed, and an annotated key to the four included genera is provided to facilitate their identification. Glenanthe is demonstrated to be a monophyletic lineage within Lipochaetini. Seven Old World species of Glenanthe are now known, including two new Afrotropical species as follows (type locality in parenthesis): G. namibia n. sp. (Namibia. Mukwe: Divuju: Okavango River [18°04’04”S, 21°28’51”E] and G. danielssoni n. sp. (Republic of South Africa. Cape Province: De Hoop Nature Reserve [34°27’S, 28°25’E]. The identity of G. fuscinervis Becker, revised status (raised from synonymy with G. ripicola (Haliday)), is clarified, and found to be conspecific with Hydrina ochracea Oldenberg, which is recognized as a junior synonym. Papp’s synonymy of Glenanthe fasciventris Becker with Hydrellia (Glenanthe) ripicola Haliday is confirmed, and herein we relegate G. iranica Canzoneri & Rampini also as junior synonym of G. ripicola. The remarkable diverse structures of the male terminalia of all included species are described and illustrated and distribution maps are also provided.