Depth-related patterns of meiofauna on the Indian continental shelf are conserved at reduced taxonomic resolution

Taxonomic sufficiency (TS) has been used in impact assessment studies of various pollution effects on marine benthic communities and found appropriate to identify the effects of pollution on marine communities. Cost, in terms of the expertise and time needed to identify organisms, increases with the level of taxonomic accuracy. Recently, TS has been adopted to study spatial patterns of macrobenthic community structure. In order to accept TS as a routine approach in wider benthic studies, it needs to be proved valid for various taxa and in geographically different areas. The present study investigates the value of TS in meiofaunal nematodes by analyzing an extensive data set based on samples collected from a wide geographical area covering a large depth gradient. For this study, samples were collected from every degree square of the western Indian continental shelf (7°–22°N latitudes). Our high resolution data showed that with increase in depth, nematode species richness and diversity decreased and communities showed significant variation between shallow and deeper waters. The present study tests whether lower taxonomic resolution nematode data can explain community shifts along a depth gradient in a similar way to species level data from the same data set. Meiofauna have often been neglected from benthic studies, and most attention has been given to macrofauna. This is mainly due to the difficulty in the taxonomic identification of meiofauna. The results of this study based on univariate and multivariate analyses support the use of family level data of nematodes to explain some aspects of depth variation in a similar way to species level data.     

Ecological regime shifts in salinised wetland systems. II. Factors affecting the dominance of benthic microbial communities

This paper is the second in a pair investigating potential mechanisms for ecological regime change in salinising wetlands. The first paper in this series focused on the responses of the salt-tolerant submerged macrophyte community to salinity. In this second paper, we investigated some of the environmental conditions required for initiation and dominance of benthic microbial communities using a combination of experimental and observational data. Two experiments were carried out. One investigated the importance of prior establishment of benthic microbial communities on their ability to maintain prevalence over macrophyte colonisation (‘persistence’ experiment), while the other investigated hydrology and its effect on sediment perturbation, potential nutrient release and subsequent benthic microbial community establishment (‘flooding’ experiment). The ‘persistence’ experiment measured the biomass of benthic microbial communities and emergence of macrophytes from sediments kept either wet or dry for 4 weeks then flooded at a range of salinities. Benthic microbial biomass was similar across all of the salinities tested (15, 45 and 70 ppt), with a slight increase at higher salinities, suggesting that none of these limited benthic microbial community development. Pre-wetting of sediments usually increased benthic microbial community biomass and reduced macrophyte germination, but the latter was attributed to the presence of anoxic sediments rather than the increased benthic microbial community biomass. Germinating macrophytes emerged through benthic microbial communities or dense heterotrophic bacterial blooms, demonstrating that they could become dominant even when another community was already established. Field data supported these results, suggesting that the development of benthic microbial communities is not limited by salinity alone, but includes other factors, such as the water regime. In the ‘flooding’ experiment, the largest differences in nutrient concentrations ultimately lay between the pre-wet and pre-dry treatments (due to the greater release of nutrients and development of anoxia in the latter) rather than those subjected to fast versus slow flooding. In response to this, highest benthic microbial community biomass was in treatments with pre-wet sediment, corresponding with lower phytoplankton biomass.     

Best Practicable Aggregation of Species: a step forward for species surrogacy in environmental assessment and monitoring

The available taxonomic expertise and knowledge of species is still inadequate to cope with the urgent need for cost‐effective methods to quantifying community response to natural and anthropogenic drivers of change. So far, the mainstream approach to overcome these impediments has focused on using higher taxa as surrogates for species. However, the use of such taxonomic surrogates often limits inferences about the causality of community patterns, which in turn is essential for effective environmental management strategies. Here, we propose an alternative approach to species surrogacy, the “Best Practicable Aggregation of Species” (BestAgg), in which surrogates exulate from fixed taxonomic schemes. The approach uses null models from random aggregations of species to minimizing the number of surrogates without causing significant losses of information on community patterns. Surrogate types are then selected in order to maximize ecological information. We applied the approach to real case studies on natural and human‐driven gradients from marine benthic communities. Outcomes from BestAgg were also compared with those obtained using classic taxonomic surrogates. Results showed that BestAgg surrogates are effective in detecting community changes. In contrast to classic taxonomic surrogates, BestAgg surrogates allow retaining significantly higher information on species‐level community patterns than what is expected to occur by chance and a potential time saving during sample processing up to 25% higher. Our findings showed that BestAgg surrogates from a pilot study could be used successfully in similar environmental investigations in the same area, or for subsequent long‐term monitoring programs. BestAgg is virtually applicable to any environmental context, allowing exploiting multiple surrogacy schemes beyond stagnant perspectives strictly relying on taxonomic relatedness among species. This prerogative is crucial to extend the concept of species surrogacy to ecological traits of species, thus leading to ecologically meaningful surrogates that, while cost effective in reflecting community patterns, may also contribute to unveil underlying processes. A specific R code for BestAgg is provided.     

Detection of environmental impacts by bottom trawling onPosidonia oceanica (L.) Delile meadows: sensitivity of fish and macroinvertebrate communities

Along the Mediterranean coast,Posidonia oceanica (L.) Delile meadows have a great ecological and economical importance. However, there is a general regression of these meadows due to human activities such as illegal bottom trawling, may be affecting to overall ecosystem health. We examined changes in the community structure of mobile fauna associated withP. oceanica meadows at different spatial scales and taxonomic levels. The aim of this paper was to identify the most efficient taxonomic level to use in environmental impact studies of bottom trawling. At the macroscale level (10 to 100 m), there were significant differences between sites in the densities of some fish species and also the total fish assemblage structure, at both family and species taxonomical levels. At the microscale (0.1 to 1 m), some species of amphipods and isopods showed significant differences in their population densities. In the overall analysis of community structure, the coarse taxonomical levels, such as phyla and class, did not show significant differences, however amphipods and isopods showed significant differences at family and species levels. From these results, both study scales are required to detect changes onPosidonia meadows' fauna. Monitoring of some fish species such asDiplodus annularis (Linnaeus, 1758) and the overall fish assemblage as well as the structure of the amphipod and isopod communities appears to be the most efficient tool in the assessment of environmental impacts by bottom trawling onP. oceanica meadows.     

Fish–environment Associations in the Coastal Waters of Andros Island, The Bahamas

Synopsis We surveyed fish communities and corresponding environmental conditions at three broadly similar coastal sites of eastern Andros Island, The Bahamas over a summer–winter–summer sequence to assess the relationship between detailed environmental features and fish species patterns. Environmental variables included covers of various benthic flora components, benthic flora diversity, floral canopy height, micro-crustacean diversity and density, water temperature, extent of destructive land-use and extent of invasion by human-introduced exotic terrestrial plants. Correspondence analysis (CA) indicated that spatial (site) differences in environmental characteristics were greater than temporal (seasonal) differences. Detrended canonical correspondence analysis (DCCA) was used to assess the strength of relationships between the environmental characteristics and the distribution patterns of 25 fish species. Environmental features deemed to be most important in influencing fish species patterns included benthic flora canopy height, extent of invasion by exotic terrestrial plants, cover of Batophora oerstedii, cover of Thalassia testudinum, turf cover, water temperature, micro-crustacean diversity, and micro-crustacean density. Based upon similarities in distribution patterns, fish species formed four clusters which, ultimately, reflected similarities in species’ feeding habits and preferences for habitats that likely maximize foraging success. We conclude that fish distribution patterns are related to environmental characteristics, and that anthropogenic coastal activity, by influencing coastal benthic characteristics, may influence the distribution and abundances of fish species in coastal habitats.     

Environmental and geographic correlates of the taxonomic structure of primate communities

Previous research has shown that both environmental and historical factors influence the taxonomic structure of animal communities; yet, the relative importance of these effects is not known for primates. Environmental characteristics shape the possible niches in a community, providing suitable habitats for some species and not others. Therefore, communities found in similar environments should display similar species compositions. Additionally, geography may be viewed as a surrogate for historical processes. For instance, as the geographic distance between communities increases, dispersal between sites is more limited, and the probability of historical vicariance increases. Therefore, communities in close proximity to each other should exhibit similar species compositions. The geographic location, environmental characteristics, and species composition of 168 primate communities were gathered from the literature. Canonical correspondence analyses were conducted to examine the relative effects of geographic distance and environmental variables on the taxonomic structure of communities. In addition, UPGMA cluster analyses were conducted to better visualize the taxonomic similarity of communities. Spatial variables were significant predictors of community structure in all regions. Rainfall patterns explained African, Malagasy, and Neotropical community structure. In addition, maximum temperature was also correlated with community structure in Madagascar and the Neotropics. No climatic variables predicted Asian community structure. These results demonstrate that both historical and environmental factors play a significant role in structuring modern primate communities; yet, the importance of environmental factors depend on the region in question. Am J Phys Anthropol, 2009. © 2008 Wiley‐Liss, Inc.     

Benthic Diatom Communities in Subalpine Pools in New Zealand: Relationships to Environmental Variables

In spite of their potential use as indicators of both present and past environmental conditions, little is known about the diatom communities in the many small water bodies at high altitudes in New Zealand. We sampled benthic diatoms at 20 sites in a typical subalpine mire pool/tarn complex near Arthur’s Pass in South Island, New Zealand in the austral spring 2001. The aims were to characterise the diatom communities, including identification of a possible endemic component, and to investigate relationships with environmental variables. The community at genus level was consistent with the peat-bog diatom flora reported from elsewhere except for the common occurrence of the Tasmania/New Zealand endemic genus Eunophora. At the species level, 27 of the 52 most common taxa appear to correspond to known species from the Northern Hemisphere and are therefore presumed to be cosmopolitan in their distribution. Just two taxa are known from the Southern Hemisphere only, however identification of the remaining common species proved problematic. Analysis using the BIO-ENV procedure of the PRIMER computer program confirmed an expected strong association between diatom community composition and pH, with water conductivity and gilvin also important. Weighted averaging regression and cross-validation using C² software enabled selection of four diatom species as potentially sensitive indicators of certain pH levels. Neither species of Eunophora showed a strong preference for pH or for any of the other environmental variables measured, indicating that other factors are determining their distributions. The strength of the species–environment relationships found in this small survey suggests good potential for monitoring current conditions and for palaeoecological applications. Extension of the dataset with information from other alpine/subalpine areas is desirable, as is the compilation of a regional diatom identification guide for these habitats.     

Metabarcoding improves detection of eukaryotes from early biofouling communities: implications for pest monitoring and pathway management

In this experimental study the patterns in early marine biofouling communities and possible implications for surveillance and environmental management were explored using metabarcoding, viz. 18S ribosomal RNA gene barcoding in combination with high-throughput sequencing. The community structure of eukaryotic assemblages and the patterns of initial succession were assessed from settlement plates deployed in a busy port for one, five and 15 days. The metabarcoding results were verified with traditional morphological identification of taxa from selected experimental plates. Metabarcoding analysis identified > 400 taxa at a comparatively low taxonomic level and morphological analysis resulted in the detection of 25 taxa at varying levels of resolution. Despite the differences in resolution, data from both methods were consistent at high taxonomic levels and similar patterns in community shifts were observed. A high percentage of sequences belonging to genera known to contain non-indigenous species (NIS) were detected after exposure for only one day.     

Urban ponds as an aquatic biodiversity resource in modified landscapes

Urbanization is a global process contributing to the loss and fragmentation of natural habitats. Many studies have focused on the biological response of terrestrial taxa and habitats to urbanization. However, little is known regarding the consequences of urbanization on freshwater habitats, especially small lentic systems. In this study, we examined aquatic macro‐invertebrate diversity (family and species level) and variation in community composition between 240 urban and 782 nonurban ponds distributed across the United Kingdom. Contrary to predictions, urban ponds supported similar numbers of invertebrate species and families compared to nonurban ponds. Similar gamma diversity was found between the two groups at both family and species taxonomic levels. The biological communities of urban ponds were markedly different to those of nonurban ponds, and the variability in urban pond community composition was greater than that in nonurban ponds, contrary to previous work showing homogenization of communities in urban areas. Positive spatial autocorrelation was recorded for urban and nonurban ponds at 0–50 km (distance between pond study sites) and negative spatial autocorrelation was observed at 100–150 km and was stronger in urban ponds in both cases. Ponds do not follow the same ecological patterns as terrestrial and lotic habitats (reduced taxonomic richness) in urban environments; in contrast, they support high taxonomic richness and contribute significantly to regional faunal diversity. Individual cities are complex structural mosaics which evolve over long periods of time and are managed in diverse ways. This facilitates the development of a wide range of environmental conditions and habitat niches in urban ponds which can promote greater heterogeneity between pond communities at larger scales. Ponds provide an opportunity for managers and environmental regulators to conserve and enhance freshwater biodiversity in urbanized landscapes whilst also facilitating key ecosystem services including storm water storage and water treatment.     

空间和环境因子对黄河口自然和淡水恢复湿地底栖动物群落的差异影响

生物多样性的形成和维持机制是生态学研究的核心问题,其中环境和空间因子在群落构建中的相对重要性是生态学家面临的重要挑战。为探究黄河口湿地底栖动物群落的关键影响因子,及环境和空间因子对底栖动物群落结构的相对调控作用。于2017年10月与2018年5月对黄河口湿地32个样点（淡水恢复湿地19个和自然湿地13个）的底栖动物和水体理化指标进行采集分析。非度量多维标度排序（NMDS）结果显示,黄河口淡水恢复湿地和自然湿地的底栖动物群落结构显著不同。典范对应分析（CCA）表明,影响淡水恢复湿地底栖动物群落结构的环境因子主要为电导率、盐度和氧化还原电位;而自然湿地底栖动物群落结构主要受pH和无机碳的影响;盐度是两类湿地底栖动物群落组成差异的关键因子。变差分解（VPA）结果显示,环境过滤对淡水恢复湿地底栖动物群落起主导作用;在自然湿地中,空间因子对底栖动物群落具有主要的调控作用,同时环境和空间因子的相互作用也至关重要。本研究明确了黄河口的自然和恢复湿地中环境和空间因素对底栖动物群落特征的相对作用,对黄河三角洲河口湿地中生物多样性的保护和生态系统管理提供参考。     

Relating macroinvertebrate community structure to environmental characteristics and sediment contamination at the scale of the St. Lawrence River

There is still no assessment of the impact of sediment chemicals and environmental conditions on macroinvertebrates at the scale of the St. Lawrence River. In order to assess these impacts in the fluvial section of the St. Lawrence River including the Montreal harbour, the community structure of macroinvertebrates using different taxonomic aggregations (genus and family) and taxa attributes (abundance, presence–absence, indicator taxa) was assessed. The goal of the study was to determine the indicator taxa of macroinvertebrates along the fluvial continuum and relate changes in macroinvertebrate community to sediment chemical conditions and environmental characteristics of habitats using variance partitioning. This study also evaluated which taxonomic level and taxa attributes of macroinvertebrates were the most suitable for bioassessment of quality of sediments and habitat environment in the St. Lawrence River. Four different macroinvertebrate assemblages were found distributed along the fluvial continuum using either abundance or presence–absence data and genus or family levels. Indicator taxa characteristic of the different macroinvertebrate communities were associated with the sediment contamination gradient. However, habitat environmental characteristics (water masses, sulphur and DOC in sediments) had more influence on macroinvertebrate assemblages than sediment contamination. Our study confirms that family level analysis can give information comparable to the genus level analysis using presence–absence or abundance of macroinvertebrates, yet a higher number of indicator taxa were detected at the genus level.     

Freshwater diatom and macroinvertebrate diversity of coastal permanent ponds along a gradient of human impact in a Mediterranean eco-region

Mediterranean coastal areas are characterised by heavily transformed landscapes and an ever-increasing number of ponds are subjected to strong alterations. Although benthic diatoms and macroinvertebrates are widely used as indicators in freshwater ecosystems, little is still known about the diatom communities of lowland freshwater ponds in the Mediterranean region, and, furthermore, there are few macroinvertebrate-based methods to assess their ecological quality, especially in Italy. This article undertakes an analysis of benthic diatom and macroinvertebrate communities of permanent freshwater ponds, selected along a gradient of anthropogenic pressures, to identify community indicators (taxa and/or metrics) useful to evaluate the effect of human impacts. A series of 21 ponds were sampled along Tyrrhenian coast in central Italy. Five of these ponds, in a good conservations status and surrounded by woodland were selected as ‘reference sites’ for macroinvertebrates and epipelic diatoms. The remaining sixteen ponds were located in an agricultural landscape subject to different levels of human impact. The total number of macroinvertebrate taxa found in each pond was significantly higher in reference sites than in both the intermediate and heavily degraded ones, whereas the diatom species richness did not result in a good community variable to evaluate the pond ecological quality. The analysis revealed a substantial difference among the compositions of diatom communities between reference ponds and degraded ponds. The former were characterised by the presence of several species belonging to genera, such as Pinnularia sp., Eunotia sp., Stauroneis sp., Neidium sp., all of which were mostly absent from degraded ponds. Furthermore, the taxonomic richnesses of some macroinvetebrate groups (Odonata, Ephemeroptera, Trichoptera, Coleoptera), and taxa composition attributes of macroinvertebrate communities (total abundance, percentages of top three dominant taxa, percentages of Pleidae, Ancylidae, Hirudinea, Hydracarina) significantly correlated with variables linked with anthropogenic pressures. The results of the investigation suggested that diatoms tended more to reflect water chemistry through changes in community structure, whereas invertebrates responded to physical habitat changes primarily through changes in taxonomic richness. The methodologies developed for the analysis of freshwater benthic diatom and macroinvertebrate communities may have a considerable potential as a tool for assessing the ecological status of this type of water body, complying with the European Union Water Framework Directive 2000/60/EC. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Guest editors: B. Oertli, R. Cereghino, A. Hull & R. Miracle Pond Conservation: From Science to Practice. 3rd Conference of the European Pond Conservation Network, Valencia, Spain, 14–16 May 2008     

Relationship between Riparian Vegetation and Stream Benthic Communities at Three Spatial Scales

We examined the influence of riparian vegetation on macroinvertebrate community structure in streams of the Upper Thames River watershed in southwestern Ontario. Thirty-three μ-basins (129–1458 ha) were used to identify land cover variables that influenced stream macroinvertebrates. Micro-basins represented the entire drainage area of study streams and were similar in stream order (first, second) and land cover (agricultural or forest; no urban). We described the structure and composition of riparian vegetation and benthic macroinvertebrate communities at the outflow reach. The nature of the land cover was quantified for the stream network buffer (30 m) and the whole μ-basin. The objective of this study was to measure the magnitude and nature of the relationship between the riparian vegetation and benthic macroinvertebrate community at the outflow reach, stream network buffer, and whole μ-basin scales. Taxon richness (including total number of Ephemeroptera, Plecoptera, and Trichoptera taxa) and Simpson’s diversity of the macroinvertebrate community all increased with increased tree cover in the riparian zone at the outflow reach scale. Simpson’s equitability was lower with greater agricultural land cover in the stream network buffer. No relationship between the macroinvertebrate community and land cover was found at the whole μ-basin scale. Analysis of the influence of land cover on stream communities within a spatial hierarchy is important for understanding the interactions of stream ecosystems with their adjacent landscapes.     

Links Between Anthropogenic Perturbations and Benthic Macroinvertebrate Assemblages in Afromontane Forest Streams in Uganda

Relationships between environmental variables and benthic macroinvertebrate assemblages were investigated among several sites that varied in disturbance history in Bwindi Impenetrable National Park, an Afromontane site in East Africa. Environmental variables were correlated with the level of past catchment disturbance – logging, agricultural encroachment, and present tourism activity. For example, sites in medium and high disturbance categories had higher values of specific conductance and lower water transparency than low disturbance category sites, these environmental variables may therefore act indicators of ecological quality of rivers. Environmental variables such as conductivity and water transparency were found to be good predictors of benthic macroinvertebrate assemblages, with anthropogenically stressed sites having lower diversity than the reference sites. Impacted sites were dominated by tolerant taxa such as chironomid and leeches, while ‘clean water’ taxa such as Ephemeroptera, Plecoptera and Trichoptera dominated at minimally impacted sites. Comparison of sites with different disturbance histories provided evidence for differences in benthic macroinvertebrate communities that reflect the state of forest restoration and recovery. We recommend quarterly monitoring of water quality to act as an early warning system of deterioration and tracking ecological recovery of previously impacted sites.     

The importance of dispersal related and local factors in shaping the taxonomic structure of diatom metacommunities

To date, little is known about the relative importance of dispersal related versus local factors in shaping microbial metacommunities. A common criticism regarding existing datasets is that the level of taxonomic resolution might be too coarse to reliably assess microbial community structure and study biogeographical patterns. Moreover, few studies have assessed the importance of geographic distance between habitats, which may influence metacommunity dynamics through its effect on dispersal rates. We applied variation partitioning analyses to 15 separate regional datasets on diatoms found in lakes in Eurasia, Africa and Antarctica. These analyses quantified the relative contributions of dispersal related and local factors in determining patterns of taxonomic turnover at the species and at the genus level. In general, results were similar at both taxonomic levels. Local environmental factors accounted for most of the explained variation (median=21%), whereas dispersal related factors were much less important (median of significant fractions=5.5% variation explained) and failed to significantly explain any variation, independent of the environmental variables, in the majority of the datasets. However, the amount of variation explained by dispersal related factors increased with increasing geographic distance and increasing taxonomic resolution. We extrapolated our regional scale observations to the global scale by combining the regional datasets into a global dataset comprising 1039 freshwater lakes from both hemispheres and spanning a geographic distance of over 19 000  km. At this global scale, taxonomic turnover was lowest in highly connected habitats, once environmental factors were partialled out. In common with many other studies of macro-organisms, these analyses showed that both dispersal related and local variables significantly contribute to the structure of global lacustrine diatom communities.     

Rare but active taxa contribute to community dynamics of benthic biofilms in glacier‐fed streams

Glaciers harbour diverse microorganisms, which upon ice melt can be released downstream. In glacier‐fed streams microorganisms can attach to stones or sediments to form benthic biofilms. We used 454‐pyrosequencing to explore the bulk (16S rDNA) and putatively active (16S rRNA) microbial communities of stone and sediment biofilms across 26 glacier‐fed streams. We found differences in community composition between bulk and active communities among streams and a stronger congruence between biofilm types. Relative abundances of rRNA and rDNA were positively correlated across different taxa and taxonomic levels, but at lower taxonomic levels, the higher abundance in either the active or the bulk communities became more apparent. Here, environmental variables played a minor role in structuring active communities. However, we found a large number of rare taxa with higher relative abundances in rRNA compared with rDNA. This suggests that rare taxa contribute disproportionately to microbial community dynamics in glacier‐fed streams. Our findings propose that high community turnover, where taxa repeatedly enter and leave the ‘seed bank’, contributes to the maintenance of microbial biodiversity in harsh ecosystems with continuous environmental perturbations, such as glacier‐fed streams.     

Different aggregation approaches in the chironomid community and the threshold of acceptable information loss

Due to the problem of identification, Chironomidae larvae, although very abundant, are often avoided or not properly used in bioassessment programs. The aim of this work was to test how different aggregation processes—taxonomic resolution and the random aggregation approach (best practicable aggregation of species—BestAgg) affect the analysis of chironomid communities regarding any information loss. The self-organizing map method, together with classification strength analysis and Spearman’s rank correlation, revealed that the genus-level and BestAgg-abundance matrix most accurately approximated the species-level community pattern. The subfamily-level dataset was ineffective at presenting the chironomid community structure, with a substantially lower concordance with the species-level dataset. The biologic environmental gradients analyses presented the same set of important environmental variables for the species-level, genus-level, and BestAgg-abundance matrix. The indicator values analysis showed that indicator genera provide information very close to that gained from species indicators. According to our results, the numeric relationship between species and higher taxa influences taxonomic scaling, limiting Chironomidae family aggregation, with acceptable information loss only up to genus level. In addition, the BestAgg approach, with the maximum level of aggregation, properly assesses the community structure and consequently describes environmental conditions.     

Taxonomic sufficiency as a useful tool for typology in a poikilohaline estuary

Taxonomic sufficiency has been used mainly to assess benthic condition, based on the assumption that taxa can be identified to a taxonomic level higher than the species level without losing the ability to detect changes related to pollution stress. Identifying taxa to a higher level reduces the expertise and time needed to identify organisms and consequently allows increased spatial and temporal replication. The usefulness of taxonomic sufficiency for typology (identification of water body types) was examined using the benthic communities of the Mondego River estuary (Portugal). Benthic samples were collected seasonally along the Northern branch of the Mondego River estuary from July 2000 to June 2001 and several environmental parameters were measured simultaneously. Cluster analysis of species data indicated three major ecological groups, mainly related to a saline gradient along the estuary. The same groups were found when taxa were aggregated to higher taxonomic levels (genus, family, order, class), except for the phylum level. The overall spatial pattern was driven by: (1) the dominance of bivalves and the occurrence of rare marine species in the Lower Estuary; (2) the dominance of polychaetes in the Middle Estuary; (3) and the dominance of arthropods in the Upper Estuary. The ability of different taxocenes to discriminate the three ecological groups was also examined. Mollusca and Bivalvia were the only taxocenes producing the same groupings, although other taxocenes (Annelida, Polychaeta, Spionidae, Arthropoda) showed a significant ability to discriminate between all three groups. Compared to using all taxa identified to the lowest possible taxonomic level, our results indicate that for typology (1) several higher taxonomic levels were sufficient (2) while few taxocenes alone were sufficient. Electronic supplementary material  The online version of this article (doi: ) contains supplementary material, which is available to authorized users.     

Do bipartite binary antagonistic and mutualistic networks have different responses to the taxonomic resolution of nodes?

1. Bipartite network analyses are increasingly being used to better understand mutualistic and antagonistic plant–insect interactions at the community level. As a result of taxonomic limitations, it is usually very difficult to identify all nodes of a network down to the species level and many studies leave some specimens identified as lower resolution taxa. Accordingly, we do not know how much a lower resolution taxonomic representation changes the network structure compared with a representation with all nodes at species level. 2. The present study aimed to test whether insect–plant networks built using different combinations of taxonomic levels can still preserve the same basic structure of networks built only with species. 3. In total, 73 bipartite published interaction networks (mutualistic and antagonistic) were selected, which were turned into binary networks and reconstructed using the nodes classified as species, genus, family or order (representing different levels of classification difficulty). The network structures were compared using their binary representations mainly using connectance, NODF (Nestedness metric based on Overlap and Decreasing Fill) and modularity. 4. The mutualistic network structure was strongly linearly related to the original network structures if all nodes were grouped up to genus level. In antagonistic networks, the structure was related to the original network only if nodes were only grouped at the species level. 5. The findings of the present study are especially helpful for comparative network studies, such as those assessing the effects of environmental gradients. For mutualistic networks, Citizen Science programmes can provide useful ecological indicators, even with its taxonomic limitations.     

Relevance of abiotic criteria used in German lake typology for macroinvertebrate fauna

For lake characterisation, top-down typologies are mostly used throughout Europe, including type criteria such as climate, lake area, catchment geology and conductivity. In Germany, a lake typology was applied comprising ecoregion, calcium concentration, Schindler’s ratio, stratification type and residence time. However, the relevance of these criteria for the macroinvertebrate fauna has not been conclusively demonstrated till now. Benthic invertebrate community data and related environmental parameters of pristine or near-pristine lakes in Germany were analysed by multivariate analysis techniques to elucidate which environmental parameters are reflected by invertebrate composition. Moreover, benthic invertebrate data were transformed to metrics expressing ecological attributes and species richness (summarising functional composition, diversity and sensitivity measures). Multivariate statistics were used to test whether information relevant to ordination was lost and whether variation decreases using metrics which combine data with ecological attributes. Analysis of lake-type criteria revealed that ecoregions and prevailing substrates were characterized by different taxonomic compositions of macroinvertebrates. In addition, a relationship was found between community composition and lake size. Creating a novel bottom-up lake typology based on ecoregions, lake size and prevailing substrate gives better separation of distinct macroinvertebrate communities and a higher level of homogeneity within groups compared to top-down typology or single environmental parameters alone, both on species and metrics data. Despite some data variation due to methodological differences (e.g. different sampling and sorting techniques) and interannual and seasonal variation in the data set, NMDS ordination presented well-separated groups of bottom-up lake types. Lake types were more precisely separated by species data than by metric data in both top-down and bottom-up typology. However, as information loss from species lists to calculated metrics is marginal, type-specific benthic invertebrate assemblages are reflected both on the species level and on the metric level. Species and metric data are both suitable for data ordination, while single environmental parameters affecting macroinvertebrate composition can best be obtained using metrics.