The Genus Rhyacophila Pictet (Trichoptera: Rhyacophilidae) in Vietnam

Currently, 20 species of the genus Rhyacophila are known from Vietnam. Of these, 17 species are distributed among eight species groups, with three species unassigned. Examination of material collected by personnel of the Royal Ontario Museum in Toronto, Canada has revealed six new species and five new records of Rhyacophila from Vietnam, distributed among six species groups, four of which are new to Vietnam. Newly described species include: Rhyacophila smithi, Rh. curriei, Rh. laocai, Rh. meyi, Rh. olahi, and Rh. fernandi. Rhyacophila bidens Kimmins, Rh. inaequalis Denning and Schmid, Rh. lhabu Schmid, Rh. malayana Banks, and Rh. rhombica Martynov are recorded from Vietnam for the first time. The females of four species, Rh. bidens, Rh. immaculata Mey, Rh. malayana Banks, and Rh. tamdaona Oláh, are described for the first time. All 31 species of Rhyacophila now known from Vietnam are included in this work, organized by branch and species group.     

Miliusa cambodgensis sp. nov. (Annonaceae) from Cambodia and M. astiana,M. ninhbinhensis spp. nov. from Vietnam

The genus Miliusa in Cambodia and Vietnam is reviewed. Cambodia and Vietnam each harbors six species of Miliusa, including three which are described as new to science: one from Cambodia (Miliusa cambodgensis sp. nov.), the other two from Vietnam (M. astiana and M. ninhbinhensis spp. nov.). In addition, a complete nomenclature and relevant information about the Miliusa species previously known from Cambodia and Vietnam are provided, including keys to the Cambodian and Vietnamese species, the designation of a lectotype for M. baillonii and the synonymization of M. balansae var. elongatoides, M. chunii and M. sinensis with M. balansae.     

Alpinia newmanii sp. nov. (Zingiberaceae) from central Vietnam

Alpinia newmanii, a new species from central Vietnam, is described and illustrated. A morphological comparison with the most closely related species and a key to the species of Alpinia subsection Catimbium in Vietnam are provided.     

The rust mycobiota of southern Africa: Species richness, composition, and affinities

The rust mycobiota (Uredinales, Basidiomycota) of southern Africa (Botswana, Namibia, and South Africa) is analysed with regard to species richness, generic composition, and similarities to the rust mycobiotas of the remaining African continent and other regions of the world. Southern Africa is home to about 546 rust species: ca 522 species have been reported from South Africa, 73 from Namibia, and less than ten from Botswana. Thirty-two species were considered to be exotics. Two hundred and twenty-five of the species are restricted to southern Africa, suggesting an endemism rate of ca 44 %. At present, the rust fungus:host ratio is 1:38.5, which is much lower than expected from other regions of the world. This low ratio may partly be due to under-exploration of the area, but the results presented here indicate that a natural paucity of rust fungi on certain, especially species-rich plant taxa centred in southern Africa and possibly environmental factors are more important reasons. The predominant genera are Puccinia and Uromyces accounting for ca 59 % of the rust species. The genera Hemileia, Phakopsora and especially Ravenelia, centred in tropical regions, are well represented and sum up to 8 % of the species. Members of Melampsoraceae and Phragmidiaceae, common in temperate regions of the Northern Hemisphere, are scarce. Most of the other 28 recorded teleomorph genera are only represented by three or less species. In an African context, most species are shared with central and east Africa (almost 16 %). Only a few species are disjunct between southern and West Africa or Madagascar. Ca 10 % of the species are shared only with other parts of the paleotropics, especially the Indian subcontinent. Disjunctions of native species with the New World, Australia/New Zealand, or Europe are rare.     

Notes on taxonomy and new taxa of Aspidistra (Ruscaceae) in the flora of Laos and Vietnam

One new species of Aspidistra, i.e. A. nutans, and one new variety, i.e. A. tillichiana var. latifolia, recently discovered in Vietnam are described and illustrated. In addition, the rank of species is proposed for A. formosa widely distributed in northern Vietnam but originally described as a variety of A. fungilliformis. Aspidistra subrotata is recorded as a new species for the flora of Laos. Data on ecology, phenology, natural variation, distribution and expected conservation status are provided for these plants.     

A new species of Melampsora on Populus yunnanensis from China

Melampsora nujiangensis, a rust fungus found on Populus yunnanensis in China, is described as a new species. Light and scanning electron microscopy with herbarium specimens of the rust fungus show that the shape of its urediniospores differs from that of other known species of Melampsora, and its urediniospore walls are thinner than the other species. Furthermore, in phylogenetic trees based on the DNA sequences (28S and ITS) the rust fungus is phylogenetically separated by high bootstrap values. These results indicate that the fungus is an isolated species among the genus Melampsora. Contribution no. 202 from the Laboratory of Plant Parasitic Mycology, Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan     

Phylogenetic relationships of sugarcane rust fungi

The phylogenetic positions of Puccinia spp. infecting sugarcane (a complex hybrid of Saccharum spp.) were determined using 38 newly generated rust sequences and 26 sequences from GenBank. Rust specimens on sugarcane were collected from 164 locations in 23 countries and identified based on light microscopy. The morphology for all samples matched that of Puccinia kuehnii or P. melanocephala, the orange and brown rust pathogens of sugarcane, respectively. Nuclear ribosomal DNA sequences (rDNA) including portions of the 5.8S rDNA, the complete internal transcribed spacer 2 (ITS2) and 5′ region of the large subunit (nLSU) rDNA were obtained for each species along with 36 additional rust taxa. Despite a shared host, the two Puccinia spp. on sugarcane are not closely related within the Pucciniales. Phylogenetic analyses place P. melanocephala most closely to P. miscanthi, P. nakanishikii, and P. rufipes infecting Miscanthus sinensis, Cymbopogon citratus, and Imperata cylindrica, respectively. Puccinia kuehnii is basal to a clade of Poaceae-infecting rusts including P. agrophila, P. polysora, P. substriata, and Uromyces setariae-italicae infecting Schizachyrium spp., Zea mays, Digitaria spp., and Urochloa mosambicensis, respectively. Light and scanning electron microscopy images highlight morphological differences distinguishing the two sugarcane-infecting species. This study confirms the separation of rust species infecting Poaceae from Cyperaceae- and Juncaceae-infecting rusts and also provides support for the presence of an additional group that includes P. kuehnii and other grass-infecting relatives.     

New species of Ophiopogon,Peliosanthes and Tupistra (Asparagaceae s.l.) in the flora of Vietnam

Five new species named Peliosanthes aperta, P. elegans, P. kenhillii, Tupistra densiflora and T. patula are described and illustrated. These species are very local in distribution and endemic to northern or southern Vietnam. Two other species, Ophiopogon ogisui and Peliosanthes griffithii, are recorded as new to the flora of Vietnam. A key to the species of Tupistra occurring in Indochina and its neighboring regions is also provided.     

Molecular assessment of Ulva (Ulvales,Chlorophyta) diversity in Vietnam including the new species U. vietnamensis

Species diversity of Ulva in Vietnam was investigated using three commonly used genetic markers, the nuclear encoded rDNA ITS region and the plastid encoded rbcL and tufA genes. Single locus species delimitation methods, complemented with morphological and ecological information resulted in the delimitation of 19 species. This diversity is largely incongruent with the traditional understanding of Ulva diversity in Vietnam. Only four species identified in this study, U. lactuca, U. reticulata, U. spinulosa, and U. flexuosa, have been previously reported, and seven species, U. ohnoi, U. tepida, U. chaugulii, U. kraftiorum, U. meridionalis, U. limnetica, and U. aragoënsis, are recorded for the first time from Vietnam. Seven genetic clusters could not be associated with species names with certainty. A new species, U. vietnamensis, is described from marine to brackish coastal areas from southern Vietnam based on its morphological and molecular distinctiveness from the currently known Ulva species. A comparison with recent molecular-based studies of Ulva diversity showed that species composition in Vietnam is similar to that of adjacent countries, including Japan, China, as well as Australia. Our study emphasizes the importance of molecular data in the assessment of Ulva diversity, and indicates that a lot of diversity may still remain to be discovered, especially in tropical regions.     

Systematics of the Hipposideros turpis complex and a description of a new subspecies from Vietnam

• 1 Hipposideros turpis is traditionally known as a species composed of three subspecies, H. t. turpis, H. t. alongensis and H. t. pendleburyi, distributed disjunctly in south‐west Japan, north‐east Vietnam and south‐west Thailand, respectively. Prior to the present study, the systematic status of forms within the species remained unclear.
• 2 Using morphological (external, bacular, cranial and dental characters), genetic and echolocation data, we demonstrate that turpis, alongensis and pendleburyi represent three distinct species, and that these species are endemic to Japan, Vietnam and Thailand, respectively. They are very distinct genetically and do not even form a monophyletic group.
• 3 We also prove that H. alongensis is composed of two subspecies, H. a. alongensis and H. a. sungi. The latter subspecies is described as new to science. To date, H. a. alongensis appears to be restricted to the Cat Ba Island of Cat Ba National Park, west Ha Long Bay, whereas H. a. sungi ssp. nov. is known from three localities in mainland northeast Vietnam. These two subspecies are distinguished by body size, molecular data and the frequency of the constant‐frequency component of their echolocation signals.

     

Caeoma tsukubaense n. sp., a rhododendron rust fungus of Japan and southern Asia, and its relationship to Chrysomyxa rhododendri

A rust fungus found in Japan on Rhododendron kaempferi, R. kiusianum, and R. dauricum has previously been identified as Chrysomyxa rhododendri. Light and scanning electron microscopy of fresh and herbarium materials of the rust fungus, however, show that the spore surface morphology differs from the urediniospores of C. rhododendri, and the spores are slightly smaller. Furthermore, the DNA sequence of the 5′-end of the large subunit of ribosomal DNA differs from that of C. rhododendri by 3%. Telia have not been found; therefore, it is redescribed as a new anamorphic species, Caeoma tsukubaense. Several specimens from North Korea, Tibet, and Nepal bearing a similar rust fungus are also included in the species.Contribution no.193 from the Laboratory of Plant Parasitic Mycology, Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan     

On some rust fungi (Uredinales) collected in an Acacia koa–Metrosideros polymorpha woodland, Mauna Loa Road, Big Island, Hawaii

Nine species of rust fungi (Uredinales) were found during a 1-day field study in an Acacia koa–Metrosideros polymorpha woodland in Volcanoes National Park on Big Island, Hawaii. Two species, both found on Acacia koa, are native (endemic) rusts whereas 7 species are nonnative on nonnative hosts, highlighting the high proportion of introduced species in the Hawaiian flora even in more or less natural habitats. One species, Uromyces linearis on Panicum repens, constitutes the first record of this rust for the Hawaiian archipelago, bringing the total to 93 species on the islands, 70 (75.3%) of which are introduced. The species records are annotated with emphasis on the geographic origin of each taxon. In addition, the study has led to the molecular reevaluation of the genus Racospermyces, indicating that it is synonymous with Endoraecium, and six new combinations are proposed for the species previously placed in Racospermyces. The high number of introduced species in Hawaii coupled with the paucity of native species when compared to other global regions is discussed.     

New species of Peliosanthes,Rohdea and Tupistra (Asparagaceae) from Laos and Vietnam

We describe and illustrate four new species, Peliosanthes choriandra, P. tatianae and Tupistra orlovii from central to northern Laos, and Rohdea filosa from northern Vietnam. These are all very local in distribution and endemic to the respective countries. We also report new localities and their ecological conditions for five other species of Peliosanthes (P. argenteostriata, P. hirsuta, P. irinae, P. micrantha and P. nivea) recently described from Laos and/or Vietnam. Furthermore, Peliosanthes nivea is recorded as new to the flora of Laos.     

Accumulation of genes for susceptibility to rust fungi for which barley is nearly a nonhost results in two barley lines with extreme multiple susceptibility

Nonhost resistance is the most common type of resistance in plants. Understanding the factors that make plants susceptible or resistant may help to achieve durably effective resistance in crop plants. Screening of 109 barley (Hordeum vulgare L.) accessions in the seedling stage indicated that barley is a complete nonhost to most of the heterologous rust fungi studied, while it showed an intermediate status with respect to Puccinia triticina, P. hordei-murini, P. hordei-secalini, P. graminis f. sp. lolii and P. coronata ff. spp. avenae and holci. Accessions that were susceptible to a heterologous rust in the seedling stage were much more or completely resistant at adult plant stage. Differential interaction between barley accessions and heterologous rust fungi was found, suggesting the existence of rust-species-specific resistance. In particular, many landrace accessions from Ethiopia and Asia, and naked-seeded accessions, tended to be susceptible to several heterologous rusts, suggesting that some resistance genes in barley are effective against more than one heterologous rust fungal species. Some barley accessions had race-specific resistance against P. hordei-murini. We accumulated genes for susceptibility to P. triticina and P. hordei-murini in two genotypes called SusPtrit and SusPmur, respectively. In the seedling stage, these accessions were as susceptible as the host species to the target rusts. They also showed unusual susceptibility to other heterologous rusts. These two lines are a valuable asset to further experimental work on the genetics of resistance to heterologous rust fungi.Electronic Supplementary Material Supplementary material is available in the online version of this article at http://dx.doi.org/10.1007/s00425-004-1319-1Abbreviations ff. spp Formae speciales - RIL Recombinant inbred line - DC Double cross - DC-S Progeny produced by selfing of double-cross plants     

Two New Lacconectus Species from Vietnam (Coleoptera; Dytiscidae)

Two new species of Lacconectus Motschulsky are described from Vietnam, Lacconectus valeriae sp. n. and L. baolocensis sp. n., and a key is given for the species described from and occurring in that country. Together with the two new species, seven species are now known from Vietnam, and the number of Lacconectus species rises to 60.     

Coping with an antagonist: the impact of a phytopathogenic fungus on the development and behaviour of two species of alpine leaf beetle

Herbivorous insects and phytopathogenic fungi often share their host plants. This creates a network of direct and indirect interactions, with far‐reaching consequences for the ecology and evolution of all three parties. In the Alps, the leaf beetles Oreina elongata and Oreina cacaliae (Coleoptera: Chrysomelidae), and the rust fungus Uromyces cacaliae (Uredinales: Pucciniaceae) are found on the same host plant, Adenostyles alliariae (Asterales: Asteraceae). We compare the impact of rust infection on these two closely‐related beetle species, one of which, O. cacaliae, is a specialist on A. alliariae, while the other, O. elongata, moves repeatedly between Adenostyles and an alternative host, Cirsium spinosissimum. Larval performance, feeding preference, oviposition choice and dispersal behaviour were studied in field and laboratory experiments. When reared on rust‐infected leaves, larvae of both beetle species had lower growth rates, lower maximum weights and longer development times. Larvae and adults discriminated among diets in feeding trials, showing a preference for discs cut from healthy leaves over those bearing a patch of sporulating rust, those from elsewhere on an infected leaf, and those from an upper leaf on an infected plant. Females of the two species differed in behaviour: in O. cacaliae they favoured healthy leaves for larviposition, while in O. elongata they showed no significant preference during oviposition. In the field, larvae and adults of both species dispersed more rapidly when placed on infected host plants. The results demonstrate that rust infection reduces the quality of the plant as a host for both Oreina species, and they combine the ability to detect systemic infection with the evolution of evasive behaviours. For these beetles, competition with a rust clearly increases the difficulty of survival in the harsh conditions of alpine environments, and may have a profound impact on the evolution of their life history traits and host plant use.