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1.
Marker-assisted selection to introgress rice QTLs controlling root traits into an Indian upland rice variety 总被引:9,自引:0,他引:9
Steele KA Price AH Shashidhar HE Witcombe JR 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(2):208-221
A marker-assisted back-crossing (MABC) breeding programme was conducted to improve the root morphological traits, and thereby
drought tolerance, of the Indian upland rice variety, Kalinga III. This variety, the recurrent parent in the MABC, had not
previously been used for quantitative trait locus (QTL) mapping. The donor parent was Azucena, an upland japonica variety from Philippines. Five segments on different chromosomes were targeted for introgression; four segments carried QTLs
for improved root morphological traits (root length and thickness) and the fifth carried a recessive QTL for aroma. Some selection
was made at non-target regions for recurrent parent alleles. We describe the selection made in three backcross (BC) generations
and two further crosses between BC3 lines to pyramid (stack) all five target segments. Pyramids with four root QTLs were obtained in eight generations, completed
in 6 years using 3,000 marker assays in a total of 323 lines. Twenty-two near-isogenic lines (NILs) were evaluated for root
traits in five field experiments in Bangalore, India. The target segment on chromosome 9 (RM242-RM201) significantly increased
root length under both irrigated and drought stress treatments, confirming that this root length QTL from Azucena functions
in a novel genetic background. No significant effects on root length were found at the other four targets. Azucena alleles
at the locus RM248 (below the target root QTL on chromosome 7) delayed flowering. Selection for the recurrent parent allele
at this locus produced early-flowering NILs that were suited for upland environments in eastern India. 相似文献
2.
P. Ajmone Marsan C. Gorni A. Chittò R. Redaelli R. van Vijk P. Stam M. Motto 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):230-243
We exploited the AFLP®1(AFLP® is a registered trademark of Keygene, N.V.) technique to map and characterise quantitative trait loci (QTLs) for grain yield and two grain-related traits of a maize segregating population. Two maize elite inbred lines were crossed to produce 229 F2 individuals which were genotyped with 66 RFLP and 246 AFLP marker loci. By selfing the F2 plants 229 F3 lines were produced and subsequently crossed to two inbred testers (T1 and T2). Each series of testcrosses was evaluated in field trials for grain yield, dry matter concentration, and test weight. The efficiency of generating AFLP markers was substantially higher relative to RFLP markers in the same population, and the speed at which they were generated showed a great potential for application in marker-assisted selection. AFLP markers covered linkage group regions left uncovered by RFLPs; in particular at telomeric regions, previously almost devoided of markers. This increase of genome coverage afforded by the inclusion of the AFLPs revealed new QTL locations for all the traits investigated and allowed us to map telomeric QTLs with higher precision. The present study has also provided an opportunity to compare simple (SIM) and composite interval mapping (CIM) for QTL analysis. Our results indicated that the method of CIM employed in this study has greater power in the detection of QTLs, and provided more precise and accurate estimates of QTL positions and effects than SIM. For all traits and both testers we detected a total of 36 QTLs, of which only two were in common between testers. This suggested that the choice of a tester for identifying QTL alleles for use in improving an inbred is critical and that the expression of QTL alleles identified may be tester-specific. 相似文献
3.
Identification of quantitative trait loci under drought conditions in tropical maize. 1. Flowering parameters and the anthesis-silking interval 总被引:13,自引:0,他引:13
J. -M. Ribaut D. A. Hoisington J. A. Deutsch C. Jiang D. Gonzalez-de-Leon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(7):905-914
Drought is an important climatic phenomenon which, after soil infertility, ranks as the second most severe limitation to maize production in developing countries. When drought stress occurs just before or during the flowering period, a delay in silking is observed, resulting in an increase in the length of the anthesis-silking interval (ASI) and in a decrease in grain yield. Selection for reduced ASI in tropical open-pollinated varieties has been shown to be correlated with improved yields under drought stress. Since efficient selection for drought tolerance requires carefully managed experimental conditions, molecular markers were used to identify the genomic segments responsible for the expression of ASI, with the final aim of developing marker-assisted selection (MAS) strategies. An F2population of 234 individuals was genotyped at 142 loci and F3 families were evaluated in the field under several water regimes for male flowering (MFLW), male sterility (STER), female flowering (FFLW) and ASI. The genetic variance of ASI increased as a function of the stress intensity, and the broad-sense heritabilites of MFLW, FFLW and ASI were high under stress conditions, being 86%, 82% and 78%, respectively. Putative quantitative trait loci (QTLs) involved in the expression of MFLW and/or FFLW under drought were detected on chromosomes 1, 2, 4, 5, 8, 9 and 10, accounting for around 48% of the phenotypic variance for both traits. For ASI, six putative QTLs were identified under drought on chromosomes 1, 2, 5, 6, 8 and 10, and together accounted for approximately 47% of the phenotypic variance. Under water stress conditions, four QTLs were common for the expression of MFLW and FFLW, one for the expression of ASI and MFLW, and four for the expression of ASI and FFLW. The number of common QTLs for two traits was related to the level of linear correlation between these two traits. Segregation for ASI was found to be transgressive with the drought-susceptible parent contributing alleles for reduced ASI (4 days) at two QTL positions. Alleles contributed by the resistant line at the other four QTLs were responsible for a 7-day reduction of ASI. These four QTLs represented around 9% of the linkage map, and were stable over years and stress levels. It is argued that MAS based on ASI QTLs should be a powerful tool for improving drought tolerance of tropical maize inbred lines. 相似文献
4.
Jie Xu Yaxi Liu Jian Liu Moju Cao Jing Wang Hai Lan Yunbi Xu Yanli Lu Guangtang Pan Tingzhao Rong 《植物学报(英文版)》2012,54(6):358-373
The control of flowering is not only important for reproduction,but also plays a key role in the processes of domestication and adaptation.To reveal the genetic architecture for flowering time and photoperiod sensitivity,a comprehensive evaluation of the relevant literature was performed and followed by meta analysis.A total of 25 synthetic consensus quantitative trait loci(QTL)and four hot-spot genomic regions were identified for photoperiod sensitivity including 11 genes related to photoperiod response or flower morphogenesis and development.Besides,a comparative analysis of the QTL for flowering time and photoperiod sensitivity highlighted the regions containing shared and unique QTL for the two traits.Candidate genes associated with maize flowering were identified through integrated analysis of the homologous genes for flowering time in plants and the consensus QTL regions for photoperiod sensitivity in maize(Zea mays L.).Our results suggest that the combination of literature review,meta-analysis and homologous blast is an efficient approach to identify new candidate genes and create a global view of the genetic architecture for maize photoperiodic flowering.Sequences of candidate genes can be used to develop molecular markers for various models of marker-assisted selection,such as marker-assisted recurrent selection and genomic selection that can contribute significantly to crop environmental adaptation. 相似文献
5.
Guohu Yang Yuling Li Qilei Wang Yuguang Zhou Qiang Zhou Bingtao Shen Feifei Zhang Xiaojie Liang 《Molecular breeding : new strategies in plant improvement》2012,29(2):313-333
Improvement in grain yield is an important objective in high-oil maize breeding. In this study, one high-oil maize inbred
was crossed with two normal maize inbreds to produce two connected recombinant inbred line (RIL) populations with 282 and
263 F7:8 families, respectively. The field experiments were conducted under four environments, and eight grain yield components and
grain oil content were evaluated. Two genetic linkage maps were constructed using 216 and 208 polymorphic SSR markers. Quantitative
trait loci (QTL) were detected for all traits under each environment and in combined analysis. Meta-analysis was used to integrate
genetic maps and detected QTL in both populations. A total of 199 QTL were detected, 122 in population 1 and 87 in population
2. Seven, 11 and 19 QTL showed consistency across five environments, across two RIL populations and with respective F2:3 generations, respectively. 183 QTL were integrated in 28 meta-QTL (mQTL). QTL with contributions over 15% were consistently
detected in 3–4 cases and integrated in mQTL. Each mQTL included 3–19 QTL related to 1–4 traits, reflecting remarkable QTL
co-location for grain yield components and oil content. Further research and marker-assisted selection (MAS) should be concentrated
on 37 consistent QTL and four genetic regions of mQTL with more than 10 QTL at bins 3.04–3.05, 7.02, 8.04–8.05 and 9.04–9.05.
Near-isogenic lines for 100-grain-weight QTL at bin 7.02–7.03, for ear-length QTL at bin 7.02–7.03 and for rows-per-ear QTL
at bin 3.08 are now in construction using MAS. Co-located candidate genes could facilitate the identification of candidate
genes for grain yield in maize. 相似文献
6.
The relationship between photosynthetic rate and yield formation processes of the newer and older maize hybrids were investigated.
Leaf area at flowering (source) and kernel number (sink) of the newer hybrids were greater than the older ones although their
light-saturated photosynthetic rate (Psat) were not greater than the older ones before flowering. After flowering, Psat and chlorophyll content of the newer hybrids declined more slowly than the older ones. They not only distributed almost all
photosynthates produced after flowering to grain but also reallocated some reserved photosynthates produced before flowering
to grain. The newer hybrids exhibited greater grain mass than the older ones mostly because they could optimally regulate
the photosynthetic rate and yield formation processes to maximize grain mass. 相似文献
7.
采用SSR标记连锁图谱和复合区间作图法在山西灌溉和干旱胁迫条件下,对玉米(Zea mays L.)自交系黄早四×掖107组合的F3群体雌雄开花间隔天数(ASI)、结穗率和籽粒产量进行了数量性状位点(QTL)定位及基因效应分析.结果表明,在两种水分处理下,ASI、结穗率与籽粒产量的相关性均达到显著水平(P<0.05).在灌溉和干旱胁迫下,分别检测到3个和2个控制ASI的QTL,位于第1、2、3和第2、5染色体上.在灌溉条件下,在第3和第6染色体上各检测到1个控制结穗率的QTL,基因作用方式呈加性或部分显性,可解释19.9%的表型变异;在干旱条件下,在第3、 7、10染色体上共检测到4个控制结穗率的QTL,基因作用方式为显性或部分显性,可解释60.4%的表型变异.在灌溉和干旱胁迫下,控制产量的QTL分别定位在第3、6、7和第1、2、4、8染色体上,基因作用方式均以加性或部分显性为主,可解释的表型变异为7.3%~22.0%.在干旱条件下,借助连锁分子标记和基因效应分析,可构建包含ASI、结穗率和产量QTL的选择指数,用于分子标记辅助育种. 相似文献
8.
Effects of nitrogen deficiency on photosynthetic traits of maize hybrids released in different years 总被引:7,自引:0,他引:7
BACKGROUND AND AIMS: New maize (Zea mays) hybrids outperformed old ones even at reduced N rates. Understanding the mechanisms of the differences in performance between newer and older hybrids under N deficiency could provide avenues for breeding maize cultivars with large yield under N deficiency, and reduce environmental pollution caused by N fertilizers. METHODS: N deficiency effects on grain weight, plant weight, harvest index, leaf area and photosynthetic traits were studied in the field for six maize hybrids released during the past 50 years to compare their tolerance and to explore their physiological mechanisms. KEY RESULTS: N deficiency decreased grain yield and plant weight in all hybrids, especially in the older hybrids. However, there was no significant difference in harvest index, rate of light-saturated photosynthesis (Psat) 20 d before flowering, leaf area or plant weight at flowering between the N-deficient and control plants of all hybrids. Dry matter production after flowering of the N-deficient plants was significantly lower than that of the control plants in all hybrids, especially in the older hybrids, and was mostly due to differences in the rate of decrease in photosynthetic capacity during this stage. The lower Psat of the older hybrids was not due to stomatal limitation, as there was no significant difference in stomatal conductance (gs) and intercellular CO2 concentration (Ci) between the hybrids. N deficiency accelerated senescence, i.e. decreased chlorophyll and soluble protein contents, after anthesis more for the earlier released hybrids than for the later ones. N deficiency decreased phosphoenolpyruvate carboxylase (PEPCase) activity significantly more in older hybrids than newer hybrids, and affected the maximal efficiency of PSII photochemistry (Fv/Fm) only in the old hybrids and at the late stage. CONCLUSIONS: Compared with older (earlier released) hybrids, newer (later released) hybrids maintained greater plant and grain weight under N deficiency because their photosynthetic capacity decreased more slowly after anthesis, associated with smaller non-stomatal limitations due to maintenance of PEPCase activity, and chlorophyll and soluble protein content. 相似文献
9.
J.-M. Ribaut C. Jiang D. Gonzalez-de-Leon G. O. Edmeades D. A. Hoisington 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(6-7):887-896
In most maize-growing areas yield reductions due to drought have been observed. Drought at flowering time is, in some cases, the most damaging. In the experiment reported here, trials with F3 families, derived from a segregating F2 population, were conducted in the field under well-watered conditions (WW) and two other water-stress regimes affecting flowering (intermediate stress, IS, and severe stress, SS). Several yield components were measured on equal numbers of plants per family: grain yield (GY), ear number (ENO), kernel number (KNO), and 100-kernel weight (HKWT). Correlation analysis of these traits showed that they were not independent of each other. Drought resulted in a 60% decrease of GY under SS conditions. By comparing yield under WW and SS conditions, the families that performed best under WW conditions were found to be proportionately more affected by stress, and the yield reductions due to SS conditions were inversely proportional to the performance under drought. Moreover, no positive correlation was observed between a drought-tolerance index (DTI) and yield under WW conditions. The correlation between GY under WW and SS conditions was 0.31. Therefore, in this experiment, selection for yield improvement under WW conditions only, would not be very effective for yield improvement under drought. Quantitative trait loci (QTLs) were identified for GY, ENO and KNO using composite interval mapping (CIM). No major QTLs, expressing more then 13% of the phenotypic variance, were detected for any of these traits, and there were inconsistencies in their genomic positions across water regimes. The use of CIM allowed the evaluation of QTL-by-environment interactions (Q×E) and could thus identify “stable” QTLs CIMMYT, Apartado Postal 6-641, 06600 Mexico D.F., Mexico across drought environments. Two such QTLs for GY, on chromosomes 1 and 10, coincided with two stable QTLs for KNO. Moreover, four genomic regions were identified for the expression of both GY and the anthesis-silking interval (ASI). In three of these, the allelic contributions were for short ASI and GY increase, while for that on chromosome 10 the allelic contribution for short ASI corresponded to a yield reduction. From these results, we hypothesize that to improve yield under drought, marker-assisted selection (MAS) using only the QTLs involved in the expression of yield components appears not to be the best strategy, and neither does MAS using only QTLs involved in the expression of ASI. We would therefore favour a MAS strategy that takes into account a combination of the “best QTLs” for different traits. These QTLs should be stable across target environments, represent the largest percentage possible of the phenotypic variance, and, though not involved directly in the expression of yield, should be involved in the expression of traits significantly correlated with yield, such as ASI. 相似文献
10.
G. Sandoya R.A. Malvar R. Santiago A. Alvarez P. Revilla A. Butrón 《The Annals of applied biology》2010,156(3):377-386
A maize synthetic population was improved for resistance to the Mediterranean corn borer (MCB, Sesamia nonagrioides) while maintaining yield. The objectives of this research were to investigate whether yield and yield stability of the maize synthetic population named EPS12 were affected by selection for MCB resistance; also to determine which genotypic and environmental covariates could explain the genotype (G), environment (E) and genotype × environment (GE) effects for yield under corn borer infestation. Plants from three cycles of selection and their testcrosses to three inbred testers (A639, B93 and EP42) were evaluated at two locations in 2 years, under MCB and European corn borer infestations. After selection EPS12 was a more stable genotype. Hybrids derived from crosses between B93 and inbreds obtained from the initial cycles of selection could be recommended for cultivation in northern Spain. The yield of crosses between cycles of selection and testers increased when there were fewer days with temperatures >25°C and higher mean maximum temperatures. Differences in yield among these genotypes were mostly explained by resistance to corn borer attack. In general, among EPS12-derived materials, genetic characteristics that contribute to increased grain yield were also responsible for increased abiotic stress tolerance. 相似文献
11.
Flachenecker C Frisch M Falke KC Melchinger AE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(6):1113-1120
Selection response of a modified recurrent full-sib (FS) selection scheme conducted in two European flint F2 maize (Zea mays L.) populations was re-evaluated. Our objectives were to (1) determine the selection response for per se and testcross performance in both populations and (2) separate genetic effects due to selection from those due to random genetic drift. Modified recurrent FS selection was conducted at three locations using an effective population size N
e = 32 and a selection rate of 25% for a selection index, based on grain yield and grain moisture. Recombination was performed according to a pseudo-factorial mating scheme. Selection response was assessed using a population diallel including the source population and advanced selection cycles, as well as testcrosses with unrelatesd inbred line testers and the parental F1 generation. Selection response per cycle was significant for grain yield and grain moisture in both populations. Effects of random genetic drift caused only a small reduction in the selection response. No significant selection response was observed for testcrosses, suggesting that for heterotic traits, such as grain yield, a high frequency of favorable alleles in the elite tester masked the effects of genes segregating in the populations. We conclude that our modified recurrent FS selection is an alternative to other commonly applied intrapopulation recurrent selection schemes, and some of its features may also be useful for increasing the efficiency of interpopulation recurrent selection programs. 相似文献
12.
QTL mapping with near-isogenic lines in maize 总被引:2,自引:0,他引:2
Szalma SJ Hostert BM Ledeaux JR Stuber CW Holland JB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(7):1211-1228
A set of 89 near-isogenic lines (NILs) of maize was created using marker-assisted selection. Nineteen genomic regions, identified
by restriction fragment length polymorphism loci and chosen to represent portions of all ten maize chromosomes, were introgressed
by backcrossing three generations from donor line Tx303 into the B73 genetic background. NILs were genotyped at an additional
128 simple sequence repeat loci to estimate the size of introgressions and the amount of background introgression. Tx303 introgressions
ranged in size from 10 to 150 cM, with an average of 60 cM. Across all NILs, 89% of the Tx303 genome is represented in targeted
and background introgressions. The average proportion of background introgression was 2.5% (range 0–15%), significantly lower
than the expected value of 9.4% for third backcross generation lines developed without marker-assisted selection. The NILs
were grown in replicated field evaluations in two years to map QTLs for flowering time traits. A parallel experiment of testcrosses
of each NIL to the unrelated inbred, Mo17, was conducted in the same environments to map QTLs in NIL testcross hybrids. QTLs
affecting days to anthesis, days to silking, and anthesis-silk interval were detected in both inbreds and hybrids in both
environments. The testing environments differed dramatically for drought stress, and different sets of QTLs were detected
across environments. Furthermore, QTLs detected in inbreds were typically different from QTLs detected in hybrids, demonstrating
the genetic complexity of flowering time. NILs can serve as a valuable genetic mapping resource for maize breeders and geneticists.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
13.
The efficiency of marker-assisted selection (MAS) depends on the power of quantitative trait locus (QTL) detection and unbiased estimation of QTL effects. Two independent samples N = 344 and 107 of F2 plants were genotyped for 89 RFLP markers. For each sample, testcross (TC) progenies of the corresponding F3 lines with two testers were evaluated in four environments. QTL for grain yield and other agronomically important traits were mapped in both samples. QTL effects were estimated from the same data as used for detection and mapping of QTL (calibration) and, based on QTL positions from calibration, from the second, independent sample (validation). For all traits and both testers we detected a total of 107 QTL with N = 344, and 39 QTL with N = 107, of which only 20 were in common. Consistency of QTL effects across testers was in agreement with corresponding genotypic correlations between the two TC series. Most QTL displayed no significant QTL x environment nor epistatic interactions. Estimates of the proportion of the phenotypic and genetic variance explained by QTL were considerably reduced when derived from the independent validation sample as opposed to estimates from the calibration sample. We conclude that, unless QTL effects are estimated from an independent sample, they can be inflated, resulting in an overly optimistic assessment of the efficiency of MAS. 相似文献
14.
Mining of Candidate Maize Genes for Nitrogen Use Efficiency by Integrating Gene Expression and QTL Data 总被引:1,自引:0,他引:1
Ruixiang Liu Hao Zhang Pu Zhao Zuxin Zhang Wenke Liang Zhigang Tian Yonglian Zheng 《Plant Molecular Biology Reporter》2012,30(2):297-308
Breeding maize varieties for high nitrogen (N) use efficiency (NUE) by marker-assisted selection using NUE quantitative trait
locus (QTL) or by genetic transfer of NUE-associated genes is a viable approach for increasing grain yield in N-limited production
areas. In this investigation, we evaluated a set of introgression line populations under N supply and N deficiency conditions.
From 42 QTLs for grain yield and yield components, 23 were identified under N supply conditions and 33 from N limited conditions.
Meta-analysis of published maize NUE QTLs revealed 37 “consensus” QTLs, of which, 18 was detected under low N conditions.
In addition, 258 unique ESTs associated with low N stress response, N uptake, transport, and assimilation were aligned on
the maize genome by in silico mapping. Integrating the EST map with the QTL map has resulted in the identification of candidate
NUE-associated genes of the following functional categories: N uptake, transport, and assimilation; carbon (C) metabolism
and assimilation; and cascades of stress response and signal transduction genes. Nine candidates that have been introgressed
into Ye478 significantly altered grain yield/yield components. It is suggested that the dynamics of interactions between C
and N metabolism are important for maize yield. A high NUE variety should have a highly efficient C assimilation per unit
N and actively express CO2 assimilation-related genes under N-limited conditions. 相似文献
15.
Development of β-Carotene Rich Maize Hybrids through Marker-Assisted Introgression of β-carotene hydroxylase Allele 总被引:1,自引:0,他引:1
Vignesh Muthusamy Firoz Hossain Nepolean Thirunavukkarasu Mukesh Choudhary Supradip Saha Jayant S. Bhat Boddupalli M. Prasanna Hari S. Gupta 《PloS one》2014,9(12)
Development of vitamin A-rich cereals can help in alleviating the widespread problem of vitamin A deficiency. We report here significant enhancement of kernel β-carotene in elite maize genotypes through accelerated marker-assisted backcross breeding. A favourable allele (543 bp) of the β-carotene hydroxylase (crtRB1) gene was introgressed in the seven elite inbred parents, which were low (1.4 µg/g) in kernel β-carotene, by using a crtRB1-specific DNA marker for foreground selection. About 90% of the recurrent parent genome was recovered in the selected progenies within two backcross generations. Concentration of β-carotene among the crtRB1-introgressed inbreds varied from 8.6 to 17.5 µg/g - a maximum increase up to 12.6-fold over recurrent parent. The reconstituted hybrids developed from improved parental inbreds also showed enhanced kernel β-carotene as high as 21.7 µg/g, compared to 2.6 µg/g in the original hybrid. The reconstituted hybrids evaluated at two locations possessed similar grain yield to that of original hybrids. These β-carotene enriched high yielding hybrids can be effectively utilized in the maize biofortification programs across the globe. 相似文献
16.
Verification of yield QTL through realized molecular marker- assisted selection responses in a barley cross 总被引:11,自引:0,他引:11
Romagosa Ignacio Han Feng Ullrich Steven E. Hayes Patrick M. Wesenberg Darrell M. 《Molecular breeding : new strategies in plant improvement》1999,5(2):143-152
Verification of putative quantitative trait loci (QTL) is an essential step towards implementing the use of marker-assisted
selection (MAS) in cultivar improvement. In a previous study with 150 doubled haploid lines derived from the 6-row cross Steptoe/Morex
(S/M), four regions (QTL1–4) of the barley genome were associated with differential genotypic expression for grain yield across
environments. The objectives of this study were to verify the value of these four QTL for selection and to compare the efficiency
of alternative MAS strategies using these QTL vs. conventional phenotypic selection for grain yield. A total of 92 DHLs derived
from the S/M cross that were not used in the original mapping efforts were used for QTL verification. Confirmation of QTL
effects was first accomplished by assessing yield differences between individuals carrying alternative alleles at each putative
locus in three environments. QTL1 on chromosome 3 was confirmed as the most important and consistent locus to determine yield
across sites, with the S allele being favorable. The M allele at QTL3 on chromosome 6 was beneficial for grain yield across
sites, but to a lesser degree than QTL1. Magnitudes of allele effects at QTL2 (chromosome 2) and QTL4 (chromosome 7) were
highly influenced by the environment where the genotypes were grown. Verification of QTL effects was best achieved by comparing
realized selection response. Genotypic (MAS) and tandem genotypic and phenotypic selection were at least as good as phenotypic
selection. Consistent selection responses were detected for QTL1 alone and together with QTL3. Genotypic selection for lines
carrying the S allele at QTL1 resulted in the identification of high-yielding genotypes. Selection responses increased when
the M allele at QTL3 was combined with the S allele at QTL1. Significant qualitative QTL × environment interactions for QTL2
and QTL4 were detected through differential realized selection responses at different sites. Without a thorough understanding
of the physiological and agronomic particulars of any QTL and the target environment, MAS for QTL showing qualitative interactions
should be minimized
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
17.
Y. N. Xiao X. H. Li M. L. George M. S. Li S. H. Zhang Y. L. Zheng 《Plant Molecular Biology Reporter》2005,23(2):155-165
Drought accounts for significant yield losses in crops. Maize (Zea mays L.) is particularly sensitive to water stress at reproductive stages, and breeding to improve drought tolerance has been
a challenge. By use of a linkage map with 121 single sequence repeat (SSR) markers, quantitative trait loci (QTLs) for grain
yield and yield components were characterized in the population of the cross X178×B73 under water-stressed and well-watered
conditions. Under the well-watered regime, 2, 4, 4, 1, 2, 2, and 3 QTLs were identified for grain yield, 100-kernel weight,
kernel number per ear, cob weight per ear, kernel weight per ear, ear weight, and ear number per plant, respectively, whereas
under the water-stressed conditions, 1, 5, 2, 6, 1, 3, and 2 QTLs, respectively, were found. The significant phenotypic correlations
among yield and yield components to some extent were observed under both water conditions, and some overlaps between the corresponding
QTLs were also found. QTLs for grain yield and kernel weight per ear under well-watered conditions and ear weight under both
well-watered and water-stressed conditions over-lapped, and all were located on chromosome 1.03 near marker bnlg176. Two other
noticeable QTL regions were on chromosome 9.05 and 9.07 near markers umc1657 and bnlg1525; the first corresponded to grain
yield, kernel weight per ear, and ear weight under well-watered conditions and kernel number per ear under both water conditions,
and the second to grain yield and cob weight per ear under water-stressed conditions and ear number per plant under both water
conditions. A comparative analysis of the QTLs herein identified with those described in previous studies for yield and yield
components in different maize populations revealed a number of QTLs in common. These QTLs have potential use in molecular
marker-assisted selection. 相似文献
18.
Drought is a major environmental constraint to crop distribution and productivity. Stem water-soluble carbohydrates (WSC) buffer wheat grain yield against conditions unfavorable for photosynthesis during the grain filling stage. In this study, 262 winter wheat accessions and 209 genome-wide SSR markers were collected and used to undertake association analysis based on a mixed linear model (MLM). The WSC in different internodes at three growth stages and 1000-grain weight (TGW) were investigated under four environmental regimes (well-watered, drought stress during the whole growth period, and two levels of terminal drought stress imposed by chemical desiccation under the well-watered and drought stress during the whole growth period conditions). Under diverse drought stress conditions, WSC in lower internodes showed significant positive correlations with TGW, especially at the flowering stage under well-watered conditions and at grain filling under drought stress. Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW. In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes. During the past fifty years of wheat breeding, WSC was selected incidentally. The average number of favorable WSC alleles increased from 1.13 in the pre-1960 period to 4.41 in the post-2000 period. The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat. 相似文献
19.
20.
Kuchel Haydn Ye Guoyou Fox Rebecca Jefferies Stephen 《Molecular breeding : new strategies in plant improvement》2005,16(1):67-78
The advent of molecular markers as a tool to aid selection has provided plant breeders with the opportunity to rapidly deliver superior genetic solutions to problems in agricultural production systems. However, a major constraint to the implementation of marker-assisted selection (MAS) in pragmatic breeding programs in the past has been the perceived high relative cost of MAS compared to conventional phenotypic selection. In this paper, computer simulation was used to design a genetically effective and economically efficient marker-assisted breeding strategy aimed at a specific outcome. Under investigation was a strategy involving the integration of both restricted backcrossing and doubled haploid (DH) technology. The point at which molecular markers are applied in a selection strategy can be critical to the effectiveness and cost efficiency of that strategy. The application of molecular markers was considered at three phases in the strategy: allele enrichment in the BC1F1 population, gene selection at the haploid stage and the selection for recurrent parent background of DHs prior to field testing. Overall, incorporating MAS at all three stages was the most effective, in terms of delivering a high frequency of desired outcomes and at combining the selected favourable rust resistance, end use quality and grain yield alleles. However, when costs were included in the model the combination of MAS at the BC1F1 and haploid stage was identified as the optimal strategy. A detailed economic analysis showed that incorporation of marker selection at these two stages not only increased genetic gain over the phenotypic alternative but actually reduced the over all cost by 40%. 相似文献