共查询到20条相似文献,搜索用时 31 毫秒
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Tsuyoshi Tanaka Yoshiaki Maeda Alaguraj Veluchamy Michihiro Tanaka Heni Abida Eric Maréchal Chris Bowler Masaki Muto Yoshihiko Sunaga Masayoshi Tanaka Tomoko Yoshino Takeaki Taniguchi Yorikane Fukuda Michiko Nemoto Mitsufumi Matsumoto Pui Shan Wong Sachiyo Aburatani Wataru Fujibuchi 《The Plant cell》2015,27(1):162-176
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Isolation and localization of a cytosolic 10 S triacylglycerol biosynthetic multienzyme complex from oleaginous yeast 总被引:3,自引:0,他引:3
Triacylglycerol is one of the major storage forms of metabolic energy in eukaryotic cells. Biosynthesis of triacylglycerol is known to occur in membranes. We report here the isolation, purification, and characterization of a catalytically active cytosolic 10 S multienzyme complex for triacylglycerol biosynthesis from Rhodotorula glutinis during exponential growth. The complex was characterized and was found to contain lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, acyl-acyl carrier protein synthetase, and acyl carrier protein. The 10 S triacylglycerol biosynthetic complex rapidly incorporates free fatty acids as well as fatty acyl-coenzyme A into triacylglycerol and its biosynthetic intermediates. Lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, and diacylglycerol acyltransferase from the complex were microsequenced. Antibodies were raised against the synthetic peptides corresponding to lysophosphatidic acid acyltransferase and phosphatidic acid phosphatase sequences. Immunoprecipitation and immunolocalization studies show the presence of a cytosolic multienzyme complex for triacylglycerol biosynthesis. Chemical cross-linking studies revealed that the 10 S multienzyme complex was held together by protein-protein interactions. These results demonstrate that the cytosol is one of the sites for triacylglycerol biosynthesis in oleaginous yeast. 相似文献
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Dongmei Wang Kang Ning Jing Li Jianqiang Hu Danxiang Han Hui Wang Xiaowei Zeng Xiaoyan Jing Qian Zhou Xiaoquan Su Xingzhi Chang Anhui Wang Wei Wang Jing Jia Li Wei Yi Xin Yinghe Qiao Ranran Huang Jie Chen Bo Han Kangsup Yoon Russell T. Hill Yonathan Zohar Feng Chen Qiang Hu Jian Xu 《PLoS genetics》2014,10(1)
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Krishna R. Kalari Brian M. Necela Xiaojia Tang Kevin J. Thompson Melissa Lau Jeanette E. Eckel-Passow Jennifer M. Kachergus S. Keith Anderson Zhifu Sun Saurabh Baheti Jennifer M. Carr Tiffany R. Baker Poulami Barman Derek C. Radisky Richard W. Joseph Sarah A. McLaughlin High-seng Chai Stephan Camille David Rossell Yan W. Asmann E. Aubrey Thompson Edith A. Perez 《PloS one》2013,8(11)
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微生物油脂是未来燃料和食品用油的重要潜在资源。近年来,随着系统生物学技术的快速发展,从全局角度理解产油微生物生理代谢及脂质积累的特征成为研究热点。组学技术作为系统生物学研究的重要工具被广泛用于揭示产油微生物脂质高效生产的机制研究中,这为产油微生物理性遗传改造和发酵过程控制提供了基础。文中对组学技术在产油微生物中的应用概况进行了综述,介绍了产油微生物组学分析常用的样品前处理及数据分析方法,综述了包括基因组、转录组、蛋白(修饰)组及代谢(脂质)组等在内的多种组学技术,以及组学数据基础上的数学模型在揭示产油微生物脂质高效生产机制中的研究,并对未来发展和应用进行了展望。 相似文献
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Enzymes of triacylglycerol synthesis and their regulation 总被引:16,自引:0,他引:16
Since the pathways of glycerolipid biosynthesis were elucidated in the 1950's, considerable knowledge has been gained about the enzymes that catalyze the lipid biosynthetic reactions and the factors that regulate triacylglycerol biosynthesis. In the last few decades, in part due to advances in technology and the wide availability of nucleotide and amino acid sequences, we have made enormous strides in our understanding of these enzymes at the molecular level. In many cases, sequence information obtained from lipid biosynthetic enzymes of prokaryotes and yeast has provided the means to search the genomic and expressed sequence tag databases for mammalian homologs and most of the genes have now been identified. Surprisingly, multiple isoforms appear to catalyze the same chemical reactions, suggesting that each isoform may play a distinct functional role in the pathway of triacylglycerol and phospholipid biosynthesis. This review focuses on the de novo biosynthesis of triacylglycerol in eukaryotic cells, the isoenzymes that are involved, their subcellular locations, how they are regulated, and their putative individual roles in glycerolipid biosynthesis. 相似文献
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Leebens-Mack J Vision T Brenner E Bowers JE Cannon S Clement MJ Cunningham CW dePamphilis C deSalle R Doyle JJ Eisen JA Gu X Harshman J Jansen RK Kellogg EA Koonin EV Mishler BD Philippe H Pires JC Qiu YL Rhee SY Sjölander K Soltis DE Soltis PS Stevenson DW Wall K Warnow T Zmasek C 《Omics : a journal of integrative biology》2006,10(2):231-237
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Genome sequencing of the high oil crop sesame provides insight into oil biosynthesis 总被引:1,自引:0,他引:1
Linhai Wang Sheng Yu Chaobo Tong Yingzhong Zhao Yan Liu Chi Song Yanxin Zhang Xudong Zhang Ying Wang Wei Hua Donghua Li Dan Li Fang Li Jingyin Yu Chunyan Xu Xuelian Han Shunmou Huang Shuaishuai Tai Junyi Wang Xun Xu Yingrui Li Shengyi Liu Rajeev K Varshney Jun Wang Xiurong Zhang 《Genome biology》2014,15(2):R39
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Eighteen new oleaginous yeast species 总被引:1,自引:0,他引:1
Luis A. Garay Irnayuli R. Sitepu Tomas Cajka Idelia Chandra Sandy Shi Ting Lin J. Bruce German Oliver Fiehn Kyria L. Boundy-Mills 《Journal of industrial microbiology & biotechnology》2016,43(7):887-900
Of 1600 known species of yeasts, about 70 are known to be oleaginous, defined as being able to accumulate over 20 % intracellular lipids. These yeasts have value for fundamental and applied research. A survey of yeasts from the Phaff Yeast Culture Collection, University of California Davis was performed to identify additional oleaginous species within the Basidiomycota phylum. Fifty-nine strains belonging to 34 species were grown in lipid inducing media, and total cell mass, lipid yield and triacylglycerol profiles were determined. Thirty-two species accumulated at least 20 % lipid and 25 species accumulated over 40 % lipid by dry weight. Eighteen of these species were not previously reported to be oleaginous. Triacylglycerol profiles were suitable for biodiesel production. These results greatly expand the number of known oleaginous yeast species, and reveal the wealth of natural diversity of triacylglycerol profiles within wild-type oleaginous Basidiomycetes. 相似文献
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Sequence and analysis of the Arabidopsis genome 总被引:2,自引:0,他引:2
Bevan M Mayer K White O Eisen JA Preuss D Bureau T Salzberg SL Mewes HW 《Current opinion in plant biology》2001,4(2):105-110
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JingJian Zhang LingLin Wan Song Xia AiFen Li ChengWu Zhang 《Bioprocess and biosystems engineering》2013,36(8):1125-1130
Eustigmatos cf. polyphem is a yellow-green unicellular edaphic microalga belonging to the eustigmatophyte. The characteristics of lipids accumulation of E. cf. polyphem grown in a bubble cylindrical photobioreactor under nitrogen-limited conditions was dissected by morphological and spectrometric analyses. Total lipids accumulation rate increased rapidly at early growth phase, with the emergence of many small lipid bodies. Afterwards, lipid bodies became abundant and enlarged primarily because of the progressive accumulation of lipids and the fusion of nearby lipid bodies. Maximum total lipids and neutral lipids content reached up to 60.59 % and 53.08 % of cell dry weight, accompanied with a biomass dry weight 7.9 g/l. E. cf. polyphem is thus referred to as an oleaginous microalga for biodiesel production due to its high biomass and considerable production of oils. 相似文献
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