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1.
Investigation of the plant growth inhibitory activity of the extract of the liverwort, Conocephalum conicum afforded five sesquiterpene lactones, tulipinolide, zaluzanin C, zaluzanin D, 8α-acetoxyzaluzanin C and 8α-acetoxy-zaluzanin D. The latter two compounds are reported for the first time. Tulipinolide is responsible for the characteristic pungency of the female gametophyte of C. conicum. The guaianolides showed inhibitory activity toward the germination and growth of roots of rice in the husk. The male gametophyte of C. conicum indicated no pungency, showing the lack of tulipinolide. 相似文献
2.
Hai-Na Yu Xin-Yan Liu Shuai Gao Xiao-Juan Han Ai-Xia Cheng Hong-Xiang Lou 《Plant Cell, Tissue and Organ Culture》2014,117(2):265-277
Liverworts are rich in phenolic compounds, including flavonoids and the distinctive type of bisbibenzyls. The biosynthesis of both types of compounds is believed to involve the phenylpropanoid pathway. Phenylalanine ammonia-lyase (PAL) is thought to be the key enzyme in the biosynthesis of bisbibenzyls and flavonoids in liverworts. In this study, PAL (designated as PaPAL) was cloned and characterized from both the cDNA and genomic DNA of the liverwort Plagiochasma appendiculatum. The full-length cDNA sequence contains 2,202 bp and is predicted to encode a protein with 733 amino acids. Sequence alignment showed that PaPAL’s predicted amino acid sequence shares more than 70 % identity with PAL sequences reported in public databases. The recombinant protein was heterologously expressed in Escherichia coli and exhibited high PAL activity, catalyzing the conversion of l-phenylalanine to trans-cinnamic acid. However, the enzyme exhibited lower activity in catalyzing the formation of p-coumaric acid from l-tyrosine. Additionally, when the thallus of P. appendiculatum was treated with abiotic stress inducers methyl jasmonate and abscisic acid, PaPAL expression was enhanced, thereby augmenting bisbibenzyl formation. These results suggest that PaPAL plays a key role in the early steps of bisbibenzyl biosynthesis and that abiotic stress can stimulate the expression of PaPAL, resulting in the accumulation of bisbibenzyls in the plant. 相似文献
3.
To identify developmental mechanisms that might have been involved in the evolution of axial sporophytes in early land plants, we examined the effects of auxin-regulatory compounds in the sporophytes of the hornwort Phaeoceros personii, the liverwort Pellia epiphylla, and the moss Polytrichum ohioense, members of the three divisions of extant bryophytes. The altered growth of isolated young sporophytes exposed to applied auxin (indole-3-acetic acid) or an auxin antagonist (p-chlorophenoxyisobutyric acid) suggests that endogenous auxin acts to regulate the rates of axial growth in all bryophyte divisions. Auxin in young hornwort sporophytes moved at very low fluxes, was insensitive to an auxin-transport inhibitor (N-[1-naphthyl]phthalamic acid), and exhibited a polarity ratio close to 1.0, implying that auxin moves by simple diffusion in these structures. Emerging liverwort sporophytes had somewhat higher auxin fluxes, which were sensitive to transport inhibitors but lacked any measurable polarity. Thus, auxin movement in liverwort sporophytes appears to result from a unique type of apolar facilitated diffusion. In young Polytrichum sporophytes, auxin movement was predominantly basipetal and occurred at high fluxes exceeding those measured in maize coleoptiles. In older Polytrichum sporophytes, acropetal auxin flux had increased beyond the level measured for basipetal flux. Insofar as acropetal and basipetal fluxes had different inhibitor sensitivities, these results suggested that moss sporophytes carry out bidirectional polar transport in different cellular pathways, which resembles the transport in certain angiosperm structures. Therefore, the three lineages of extant bryophytes appear to have evolved independent innovations for auxin regulation of axial growth, with similar mechanisms operating in moss sporophytes and vascular plants. 相似文献
4.
Some properties of a preparation of an enzyme, lunularic acid decarboxylase, from the liverwort Conocephalum conicum are described. The enzyme is normally bound and could be solubilized with Triton X-100; at least some of the bound decarboxylase activity appears to be associated with chloroplasts. For lunularic acid the enzyme has Km 8.7 × 10?5 M (pH 7.8 and 30°). Some substrate analogues have been tested but no other substrate was found. Pinosylvic acid is a competitive inhibitor for the enzyme, Ki 1.2 × 10?4 M (pH 7.8 and 30°). No product inhibition was observed. Lunularic acid decarboxylase activity has also been observed with a cell-free system from Lunularia cruciata. 相似文献
5.
Lunularic acid (LNA) was isolated from the cultured cells of the liverwort Marchantia polymorpha. Quantitative analysis by reverse phase HPLC showed that the content of LNA in the cells changed markedly during their growth, ranging from 1 to 7μg/mg dry wt. The accumulation of LNA was greatly enhanced by a deficiency of phosphate in the culture medium. 相似文献
6.
《Phytomedicine》2015,22(1):77-85
Five dammarane-type triterpenoids, five pentacyclic triterpenoids (three of them carrying a carboxylic acid group), and two aromadendrane-type sesquiterpenoids were isolated from an Argentinian collection of the liverwort Lepidozia chordulifera. Compounds were characterized by comparison of their spectral data with those previously reported and tested in their ability to control bacterial growth, biofilm formation, bacterial Quorum Sensing process (QS), and elastase activity of Pseudomonas aeruginosa, as well as bacterial growth and biofilm formation of Staphylococcus aureus. The key role played by biofilm and elastase activity in bacterial virulence make them a potential target for the development of antibacterial agents. The aromadendrane-type sesquiterpenoid viridiflorol was the most potent biofilm formation inhibitor, producing 60% inhibition in P. aeruginosa and 40% in S. aureus at 50 µg/ml. Ursolic and betulinic acids (two of the pentacyclic triterpenoids isolated) were able to reduce 96 and 92% the elastase activity of P. aeruginosa at 50 µg/ml, respectively. Among the analyzed triterpenoids, those that carry a dammarane skeleton were the most potent inhibitors of the P. aeruginosa biofilm formation and were active against both P. aeruginosa and S. aureus. Subsequently, a computer-assisted study of the triterpenoid compounds was carried out for a better understanding of the structure-activity relationships. 相似文献
7.
J.S. Gray 《Journal of invertebrate pathology》1982,39(3):413-415
Seven strains of the insect pathogenic fungus Verticillium lecanii have been examined in the Calliphora erythrocephala bioassay for the production, in surface culture on Czapek Dox medium, of insecticidal secondary metabolites. One strain which had lost its pathogenicity on storage yielded no active compounds. The remainder yielded dipicolinic acid (pyridine-2,6-dicarboxylic acid) which was responsible for the insecticidal activity of acidic extracts. Neutral extracts from two strains contained novel insecticidal C25 compounds. 相似文献
8.
《Phytochemistry》2012
Three genes homologous to plant lipoxygenase genes were identified from the EST libraries of Marchantia polymorpha, in order to clarify the function of LOXs in bryophytes. Full-length genes were isolated using 5′- and 3′-RACE methods and named MpLOX1, MpLOX2, and MpLOX3, respectively. To investigate the enzymatic activities of liverwort LOXs, recombinant MpLOX1, MpLOX2, and MpLOX3 proteins were prepared from Escherichia coli cells expressing the corresponding gene. LC–MS/MS analyses and chiral column chromatography of their reaction products showed that MpLOX1 codes for 11S/15S-lipoxygenase against eicosapentaenoic acid and for 15S-lipoxygenase against arachidonic acid, and that MpLOX2 and MpLOX3 code for 15S-lipoxygenase against eicosapentaenoic and arachidonic acids. Phylogenetic analysis showed that the liverwort lipoxygenase genes separated from the ancestor of higher plants in the early stages of plant evolution. Quantification analyses suggested that arachidonic acid and eicosapentaenoic acid were preferred substrates. Furthermore, each liverwort lipoxygenase exhibited highest activity at pH 7.0 and dependency on Ca2+ ion in the oxygenation reaction. 相似文献
9.
《Phytochemistry》1987,26(3):735-738
3-Undecyl phenol, 6-undecyl salicylic acid and potassium 6-undecyl salicylate were isolated from the New Zealand liverwort Schistochila appendiculata. 3-Tridecyl and 3-pentadecyl phenol, 6-tridecyl and 6-pentadecyl salicylic acid, potassium 6-tridecyl and 6-pentadecyl salicylate and 6-undecyl catechol were also detected in the same species. The chemical constitution of S. appendiculata is quite similar to that of the brown algae Caulocystis species, and of the higher plants Ginkgo biloba and some of the Anacardia species. The allergic reaction brought on by S. appendiculata might be due to the presence of these phenolic compounds. 相似文献
10.
Ascorbate peroxidase (APX) of the liverwort Pallavicinia lyelli was extracted and purified through ammonium sulfate precipitation, Butyl-Toyopearl, DEAE-Cellulofine and Sephadex G-75 chromatography. The purification factor for APX was 285 with 7.9% yield. The enzyme was characterized for thermal stability, pH and kinetic parameters. The molecular mass of APX was approximately 28 kDa estimated by SDS-PAGE. The purity was checked by native PAGE, showing a single prominent band. The optimum pH was 6.0. The enzyme had a temperature optimum at 40 °C and was relatively stable at 60 °C, with 54% loss of activity. When the enzyme was diluted with the ascorbate-deleted medium, the half inactivation time was approximately 15 min. The absorption spectra of the purified enzyme and the inhibition by cyanide and azide showed that it is a hemoprotein. Spectral analysis and inhibitor studies were consistent with the presence of a heme moiety. When compared with ascorbate peroxidase activity derived from ruptured intact chloroplasts, the purified enzyme was found to have a higher stability, a broader pH optimum for activity and the capacity to utilize alternate electron donors. p-chloromercuribenzoate (pCMB), hydroxyurea and salicylic acid (SA) significantly inhibited APX activity. Ascorbate (AsA) and pyrogallol were found to be efficient substrates for Pallavicinia APX, considering the Vmax/Km ratio. We detected the activity of monodehydroascorbate reductase (MDHAR) involved in the regeneration of ascorbate, but failed to detect the dehydroascorbate reductase (DHAR) activity. The data obtained in this study may help to understand desiccation tolerance mechanism in the liverwort. 相似文献
11.
Fabón G Monforte L Tomás-Las-Heras R Núñez-Olivera E Martínez-Abaigar J 《Journal of plant physiology》2012,169(1):20-26
We studied the diel responses of the liverwort Jungermannia exsertifolia subsp. cordifolia to radiation changes under laboratory conditions. The samples were exposed to three radiation regimes: P (only PAR), PA (PAR + UV-A), and PAB (PAR + UV-A + UV-B). The day was divided in four periods: darkness, a first low-PAR period, the high-PAR plus UV period, and a second low-PAR period. After 15 days of culture, we measured photosynthetic pigments, chlorophyll fluorescence and UV-absorbing compounds in the four periods of the day on two consecutive days. With respect to UV-absorbing compounds, we analyzed their global amount (as the bulk UV absorbance of methanolic extracts) and the concentration of seven hydroxycinnamic acid derivatives, both in the soluble (mainly vacuolar) and insoluble (cell wall-bound) fractions of the plant extracts. PAB samples increased the bulk UV absorbance of the soluble and insoluble fractions, and the concentrations of p-coumaroylmalic acid in the soluble fraction and p-coumaric acid in the cell wall. Most of these variables showed significant diel changes and responded within a few hours to radiation changes (more strongly to UV-B), increasing at the end of the period of high-PAR plus UV. Fv/Fm, ΦPSII, NPQ and the components of the xanthophyll cycle showed significant and quick diel changes in response to high PAR, UV-A and UV-B radiation, indicating dynamic photoinhibition and protection of PSII from excess radiation through the xanthophyll cycle. Thus, the liverwort showed a dynamic protection and acclimation capacity to the irradiance level and spectral characteristics of the radiation received. 相似文献
12.
《Phytochemistry》1976,15(7):1139-1143
The cutin acids of two species of Sphagnum moss and two species of liverwort from the order Marchantiales have been analysed. The liverwort species contain as a bound lipid, 15-hydroxyhexadecanoic acid, hitherto unreported in higher plants. The relative abundances of 8,16-, 9,16- and 10,16-dihydroxyhexadecanoic acids may be of value in the chemotaxonomy of Sphagnum mosses. Based on their cutin acid compositions, mosses and liverworts may be less related than generally supposed, the Sphagnum cutins resembling more those of angiosperms and gymnosperms. The n-alkane content of Sphagnum is very low, but there are quantitative differences between species. 相似文献
13.
《Bioscience, biotechnology, and biochemistry》2013,77(5):862-863
A number of analogs of lunularic acid varying in the number of methylene carbons between the two benzene rings and in the substituents on their rings were prepared, and their effects on the growth of liverwort gemmaling, watercress, and timothy grass were investigated. Almost all the analogs tested were more inhibitory than lunularic acid, and a correlation between the structure and activity was observed. The differences in the growth-inhibition activity of analogs between higher and lower plants are also discussed. 相似文献
14.
Daniel S. Wenholz Ming Zeng Cong Ma Marcin Mielczarek Xiao Yang Mohan Bhadbhade David St. C. Black Peter J. Lewis Renate Griffith Naresh Kumar 《Bioorganic & medicinal chemistry letters》2017,27(18):4302-4308
Knoevenagel condensation was employed to generate a set of molecules potentially capable of inhibiting the RNA polymerase-σ70/σA interaction in bacteria. Synthesis was achieved via reactions between a variety of indole-7-carbaldehydes and rhodanine, N-allylrhodanine, barbituric acid or thiobarbituric acid. A library of structurally diverse compounds was examined by enzyme-linked immunosorbent assay (ELISA) to assess the inhibition of the targeted protein–protein interaction. Inhibition of bacterial growth was also evaluated using Bacillus subtilis and Escherichia coli cultures. The structure–activity relationship studies demonstrated the significance of particular structural features of the synthesized molecules for RNA polymerase-σ70/σA interaction inhibition and antibacterial activity. Docking was investigated as an in silico method for the further development of the compounds. 相似文献
15.
Robert J. Thomas Fredrich J. Behringer Craig S. Lombard Jason J. Sparkowski 《Physiologia plantarum》1984,60(4):502-506
Auxin-induced changes in cell wall polysaccharide composition and enzyme activity of seta segments from the liverwort Pellia epiphylla (L.) Corda were studied using colorimetric, gas chromatographic, radioactive tracer, and viscometric techniques. Extension-growth of segments doubled in the presence of aqueous 10 μ M indole-3-acctic acid (IAA) ± 50 m M glucose. IAA-enhanced growth was accompanied by (1) enhanced synthesis of all wall polysaccharides but cellulose, (2) increase in the relative glucose content of neutral wall sugars, and (3) change in the activity of wall-bound glycosidase relative to controls, but no change in the activity of cellulase. Galactose and mannose (50 m M ) suppressed auxin enhancement of both growth and wall synthesis. These findings suggest that auxin-mediated extension-growth of Pellia setae is dependent upon the maintenance of non-cellulosic cell-wall synthesis. 相似文献
16.
The growth and development of final-stadium tabacco hornworm Manduca sexta (Sphingidae) larvae fed a 2.5 mM l-canavanine-containing diet is disrupted markedly. Such canavanine-mediated disruption of larval growth is intensified greatly when these organisms are fed a canavanine-containing diet supplemented with a 1 : 10 molar ratio of l-arginine, l-citrulline, l-ornithine or l-2,4-diaminobutyric acid, the larvae possess enhanced haemolymph volume (oedema) and a significant mortality results from incomplete larval-pupal ecdysis. Two other compounds, 3-aminobutyric acid and l-2,3-diaminopropionic acid, do not produce larvae showing oedema but most larvae fail to complete larval-pupal ecdysis. 4-Aminobutyric acid, l-threonine and l-glutamic acid are much less potent but they still manifest appreciable developmental aberrations. Eighteen other tested compounds have no discernible effect. In general, compounds accentuating the biological activity of canavanine have: an α-carboxyl and α-amino group; a carbon skeleton of no less than 2 nor more than 4 carbon atoms; and and ω-amino group. 相似文献
17.
Prostaglandins are biologically active substances used in a wide range of medical treatments. Prostaglandins have been supplied mainly by chemical synthesis; nevertheless, the high cost of prostaglandin production remains a factor. To lower the cost of prostaglandin production, we attempted to produce prostaglandins using a liverwort, Marchantia polymorpha L., which accumulates arachidonic acid, which is known as a substrate of prostaglandins. Here we report the first bioproduction of prostaglandins in plant species by introducing a cyclooxygenase gene from a red alga, Gracilaria vermiculophylla into the liverwort. The transgenic liverworts accumulated prostaglandin F2α, prostaglandin E2 and prostaglandin D2 which were not detected in the wild-type liverwort. Moreover, we succeeded in drastically increasing the bioproduction of prostaglandins using an in vitro reaction system with the extracts of transgenic liverworts. 相似文献
18.
Mechanism of specific inhibition of phototropism by phenylacetic Acid in corn seedling 总被引:3,自引:1,他引:2
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Using geotropism as a control for phototropism, compounds similar to phenylacetic acid that photoreact with flavins and/or have auxin-like activity were examined for their ability to specifically inhibit phototropism in corn seedlings using geotropism as a control. Results using indole-3-acetic acid, napthalene-1-acetic acid, naphthalene-2-acetic acid, phenylacetic acid, and β-phenylpyruvic acid suggest that such compounds will specifically inhibit phototropism primarily because of their photoreactivity with flavins and not their auxin activity. For example, strong auxins, indole-3-acetic acid and naphthalene-1-acetic acid, affected both tropic responses at all concentrations tested whereas weak auxins, phenylacetic acid and naphthalene-2-acetic acid, exhibited specific inhibition. In addition, the in vivo concentration of phenylacetic acid required to induce specificity was well below that required to stimulate coleoptile growth. Estimates of the percentage of photoreceptor pigment inactivated by phenylacetic acid (>10%) suggest that phenylacetic acid could be used to photoaffinity label the flavoprotein involved in corn seedling phototropism. 相似文献
19.
Inactivity of 3-methyleneoxindole as mediator of auxin action on cell elongation 总被引:4,自引:3,他引:1
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The recently reported growth-promoting ability of 3-methyl-eneoxindole was examined in order to test the hypothesis that indole-3-acetic acid acts as a growth promoter only after oxidative conversion to 3-methyleneoxindole. Methyleneoxindole was synthesized from indole-3-acetic acid and N-bromosuccinimide, and its identity was confirmed by ultraviolet absorption, infrared absorption, mass spectrometry, and melting point. Methyleneoxindole was found to lack growth-promoting activity in coleoptile and pea (Pisum sativum) stem segments. Chlorogenic acid, an inhibitor of the oxidation of indole-3-acetic acid, was found to have no inhibitory effect on growth promotion by indole-3-acetic acid. It is concluded that 3-methyleneoxindole is inactive as a growth promoter and therefore does not mediate the action of auxin on cell elongation. 相似文献