14.3 Seconds: Politics,Art and the Archival Imagination

This article addresses John Greyson's 14.3 Seconds [2008 2008 14.3 Seconds. Iraq/Canada; digital video, color, 9 mins. [Google Scholar]], a video about the destruction of Iraqi film archives. This video continues the history of radical form and fabulation that characterizes Greyson's queer film/video ?uvre while also engaging the provocative experimentations with archives and history that animate recent Arab visual culture. The essay argues that 14.3 Seconds and a recent video, Prison Arabic in 50 Days [2013 Greyson, John, and Tarek Loubani 2013 Interview with Jian Ghomeshi. Q. Canadian Broadcasting Corporation. October 15; https://www.youtube.com/watch?v=JdMuXk01xeQ (accessed February 28, 2015). [Google Scholar]], use redaction, translation, archives and queer aesthetics to create new possibilities for the visualization of transnational solidarity and activism.     

Engaging with Dementia: Moral Experiments in Art and Friendship

The box-office as well as critical success of the 2014 major motion picture Still Alice, starring Julianne Moore in the title role and based on the bestselling novel of the same name by the Harvard-trained neuroscientist Lisa Genova (Still Alice. Simon & Schuster, New York, 2009), marked an important moment in public cultural representations of people with dementia. Still Alice tells the story of Alice Howland, an eminent scientist whose increasing memory lapses are eventually diagnosed as early-onset Alzheimer’s, and chronicles the transformations in her family relationships as her husband and three children respond to her decline in different ways. Alice’s husband, her son, and her older daughter all respond by turning toward science, while her younger daughter Lydia seeks to engage her mother as she is now, and turns toward art and relationships. Taking Still Alice and the figure of Lydia as an entry point, I discuss arts-focused efforts to improve the lives of people with dementia, and draw upon ongoing interview-based research on the topic of dementia and friendship, to offer an account of some of the ways that people I have spoken with are actively experimenting with art and with relationships in the face of dementia. I argue that these efforts can be understood as “moral experiements,” in the sense articulated by Cheryl Mattingly (Moral Laboratories: Family Peril and the Struggle for a Good Life. University of California Press, Berkeley, 2014). Although Lydia is a fictional character, her response to Alice’s dementia points toward the kinds of moral experimentation that are in fact possible, and quietly being practiced, by ordinary people every day.     

Evolution of the B3 DNA Binding Superfamily: New Insights into REM Family Gene Diversification

Background

The B3 DNA binding domain includes five families: auxin response factor (ARF), abscisic acid-insensitive3 (ABI3), high level expression of sugar inducible (HSI), related to ABI3/VP1 (RAV) and reproductive meristem (REM). The release of the complete genomes of the angiosperm eudicots Arabidopsis thaliana and Populus trichocarpa, the monocot Orysa sativa, the bryophyte Physcomitrella patens,the green algae Chlamydomonas reinhardtii and Volvox carteri and the red algae Cyanidioschyzon melorae provided an exceptional opportunity to study the evolution of this superfamily.

Methodology

In order to better understand the origin and the diversification of B3 domains in plants, we combined comparative phylogenetic analysis with exon/intron structure and duplication events. In addition, we investigated the conservation and divergence of the B3 domain during the origin and evolution of each family.

Conclusions

Our data indicate that showed that the B3 containing genes have undergone extensive duplication events, and that the REM family B3 domain has a highly diverged DNA binding. Our results also indicate that the founding member of the B3 gene family is likely to be similar to the ABI3/HSI genes found in C. reinhardtii and V. carteri. Among the B3 families, ABI3, HSI, RAV and ARF are most structurally conserved, whereas the REM family has experienced a rapid divergence. These results are discussed in light of their functional and evolutionary roles in plant development.     

Disciplinary Anthropology? Amateur Ethnography and the Production of ‘Heritage’ in Rural France

‘Amateur’ anthropology and ethnography are utopian categories proposed by anthropologists seeking to critique a perceived culture of ‘professionalism’ within the discipline (Grimshaw &; Hart, 1993 Grimshaw, Anna and Hart, Keith. 1993. Anthropology and the Crisis of the Intellectuals, Cambridge: Prickly Pear Pamphlet No.1.  [Google Scholar]). Yet they have arguably been practised extensively by local intellectuals oblivious to such debates. In rural Europe, this has often involved ‘pastoral’ conservation of ‘local history’, ‘traditions’ and ‘folk customs’, in the context of identity politics. Recent manifestations, however, have enabled the disciplining of cultural practices of indigenous populations by local entrepreneurs for use in heritage tourism. Building on Foucault's concept of a ‘disciplinary programme’, this paper analyses projects from a French Mediterranean village that have ‘borrowed’ discursive forms from French ethnology and historiography to convert local heritages into disciplined archives and booklets, predominantly for use in tourism. It then analyses their approximation to the discipline of anthropology; assesses their problematic distinction from anthropology's own disciplinary programmes; and explores the implications.     

Alzheimer’s disease: analysis of a mathematical model incorporating the role of prions

We introduce a mathematical model of the in vivo progression of Alzheimer’s disease with focus on the role of prions in memory impairment. Our model consists of differential equations that describe the dynamic formation of \(\upbeta \) -amyloid plaques based on the concentrations of A \(\upbeta \)  oligomers, PrP^C proteins, and the A \(\upbeta \) - \(\times \) -PrP^Ccomplex, which are hypothesized to be responsible for synaptic toxicity. We prove the well-posedness of the model and provided stability results for its unique equilibrium, when the polymerization rate of \(\upbeta \) -amyloid is constant and also when it is described by a power law.     

Visual Anthropology and Language

This essay addresses three questions: (1) What happens to information that cannot be recorded on film or videotape? (2) Is the “visual image” conceived to be an autonomous, universalized object of study, as if it exists prior to and independent of conventional human languages? (3) Who are the much-talked-about viewers of ethnographic films, and what is their relationship to visual anthropologists' investigations? The essay's focus is on Grimshaw's statement about visual anthropologists who “seek legitimation by turning away from the mainstream textual tradition” [Grimshaw 2001 Grimshaw , Anna 2001 The Ethnographer's Eye: Ways of Seeing in Modern Anthropology . Cambridge : Cambridge University Press .[Crossref] , [Google Scholar]: 172]. I have attempted to submit evidence that such a move weakens visual anthropologists' capacity to provide “mainstream anthropology” with a vital, growing and legitimate contribution.     

The “Other” in Film: Exclusions of Aboriginal Identity from Australian Cinema

Throughout the latter half of the past century, cinema played a significant role in the shaping of the core narratives of Australia. Films express and implicitly shape national images and symbolic representations of cultural fictions in which ideas about Aboriginal identity have been embedded. In this article, ¹ This article is based on a paper presented at the Australian Sociological Association's Annual Conference, in Auckland, 2007. The author wishes to thank the University of Wollongong and acknowledges its support in the presentation of this material. exclusionary practices in Australian narratives are analyzed through examples of films representing Aboriginal identity. Through these filmic narratives the articulation, interrogation, and contestation of views about filmic representations of Aboriginal identity in Australia are illuminated. The various themes in the filmic narratives are examined in order to compare and contrast the ways in which Australian films display the operation of narrative closure and dualisms within the film texts.     

To whom and to what is research on migration a contribution

Nicaraguan immigration to Costa Rica is a major case of South–South migration in Latin America. This chapter explores a set of examples of analyses of critical interventions – regarding immigration law, social imaginaries around which representations of Nicaraguans are framed, and participatory work carried out with impoverished communities – in order to reflect on the ways in which social sciences in Costa Rica attempts to intervene both in the everyday hostility of Costa Rican society and in the ways in which Nicaraguans contest that hostility. Responding to Michael Burawoy's (2005 Burawoy, Michael 2005 ‘For public sociology’, American Sociological Review, vol. 70, pp. 4–28. doi:10.1177/000312240507000102[Crossref], [Web of Science ®] , [Google Scholar], 2007) call for a ‘public sociology’, the chapter reflects on how debates around public social sciences could enrich the political, institutional and conceptual location of migration studies in Costa Rica.     

Shooting Melanesians: Martin Johnson and Edward Salisbury in the Southwest Pacific

By the turn of the 20th century American photographers were venturing into the western Pacific. Two of the first cinematic teams to film in Melanesia were the Kansan explorers Martin and Osa Johnson and the yachtsman Edward A. Salisbury, who was joined by Merian Cooper (of later King Kong fame). Both drew on representational practice honed partly along the American Western Frontier. Both pairs took still and motion pictures in the New Hebrides and Solomon Islands which they used to illustrate magazine articles, travelogue books and silent films, including the Johnsons’ Cannibals of the South Seas [1918 Johnson, Martin 1918 Cannibals of the South Seas. New York: Martin Johnson Film Co; silent, b &; w; sections available at the Martin and Osa Johnson Safari Museum archives. [Google Scholar]] and Head Hunters of the South Seas [1922 1922 Cannibal-Land: Adventures with a Camera in the New Hebrides. Boston and New York: Houghton Mifflin Company. [Google Scholar]], and Salisbury and Cooper's Gow the Head Hunter [1928 1928 Gow the Head Hunter. Hollywood: Salisbury Productions; silent, b &; w, 61 mins.; available from Flicker Alley. [Google Scholar]]. Differences in their print and motion imagery of islanders reflect the newer movie aesthetic, stimulating new ways to shoot Melanesians as spectators, as actors and as occasional filmmakers themselves.     

The Conserved miR-51 microRNA Family Is Redundantly Required for Embryonic Development and Pharynx Attachment in Caenorhabditis elegans

A major question about cytokinesis concerns the role of the septin proteins, which localize to the division site in all animal and fungal cells but are essential for cytokinesis only in some cell types. For example, in Schizosaccharomyces pombe, four septins localize to the division site, but deletion of the four genes produces only a modest delay in cell separation. To ask if the S. pombe septins function redundantly in cytokinesis, we conducted a synthetic-lethal screen in a septin-deficient strain and identified seven mutations. One mutation affects Cdc4, a myosin light chain that is an essential component of the cytokinetic actomyosin ring. Five others cause frequent cell lysis during cell separation and map to two loci. These mutations and their dosage suppressors define a signaling pathway (including Rho1 and a novel arrestin) for repairing cell-wall damage. The seventh mutation affects the poorly understood RNA-binding protein Scw1 and severely delays cell separation when combined either with a septin mutation or with a mutation affecting the septin-interacting, anillin-like protein Mid2, suggesting that Scw1 functions in a pathway parallel to that of the septins. Taken together, our results suggest that the S. pombe septins participate redundantly in one or more pathways that cooperate with the actomyosin ring during cytokinesis and that a septin defect causes septum defects that can be repaired effectively only when the cell-integrity pathway is intact.THE fission yeast Schizosaccharomyces pombe provides an outstanding model system for studies of cytokinesis (McCollum and Gould 2001; Balasubramanian et al. 2004; Pollard and Wu 2010). As in most animal cells, successful cytokinesis in S. pombe requires an actomyosin ring (AMR). The AMR begins to assemble at the G₂/M transition and involves the type II myosin heavy chains Myo2 and Myp2 and the light chains Cdc4 and Rlc1 (Wu et al. 2003). Myo2 and Cdc4 are essential for cytokinesis under all known conditions, Rlc1 is important at all temperatures but essential only at low temperatures, and Myp2 is essential only under stress conditions. As the AMR constricts, a septum of cell wall is formed between the daughter cells. The primary septum is sandwiched by secondary septa and subsequently digested to allow cell separation (Humbel et al. 2001; Sipiczki 2007). Because of the internal turgor pressure of the cells, the proper assembly and structural integrity of the septal layers are essential for cell survival.Septum formation involves the β-glucan synthases Bgs1/Cps1/Drc1, Bgs3, and Bgs4 (Ishiguro et al. 1997; Le Goff et al. 1999; Liu et al. 1999, 2002; Martín et al. 2003; Cortés et al. 2005) and the α-glucan synthase Ags1/Mok1 (Hochstenbach et al. 1998; Katayama et al. 1999). These synthases are regulated by the Rho GTPases Rho1 and Rho2 and the protein kinase C isoforms Pck1 and Pck2 (Arellano et al. 1996, 1997, 1999; Nakano et al. 1997; Hirata et al. 1998; Calonge et al. 2000; Sayers et al. 2000; Ma et al. 2006; Barba et al. 2008; García et al. 2009b). The Rho GTPases themselves appear to be regulated by both GTPase-activating proteins (GAPs) and guanine-nucleotide-exchange factors (GEFs) (Nakano et al. 2001; Calonge et al. 2003; Iwaki et al. 2003; Tajadura et al. 2004; Morrell-Falvey et al. 2005; Mutoh et al. 2005; García et al. 2006, 2009a,b). In addition, septum formation and AMR function appear to be interdependent. In the absence of a normal AMR, cells form aberrant septa and/or deposit septal materials at random locations, whereas a mutant defective in septum formation (bgs1) is also defective in AMR constriction (Gould and Simanis 1997; Le Goff et al. 1999; Liu et al. 1999, 2000). Both AMR constriction and septum formation also depend on the septation initiation network involving the small GTPase Spg1 (McCollum and Gould 2001; Krapp and Simanis 2008). Despite this considerable progress, many questions remain about the mechanisms and regulation of septum formation and its relationships to the function of the AMR.One major question concerns the role(s) of the septins. Proteins of this family are ubiquitous in fungal and animal cells and typically localize to the cell cortex, where they appear to serve as scaffolds and diffusion barriers for other proteins that participate in a wide variety of cellular processes (Longtine et al. 1996; Gladfelter et al. 2001; Hall et al. 2008; Caudron and Barral 2009). Despite the recent progress in elucidating the mechanisms of septin assembly (John et al. 2007; Sirajuddin et al. 2007; Bertin et al. 2008; McMurray and Thorner 2008), the details of septin function remain obscure. However, one prominent role of the septins and associated proteins is in cytokinesis. Septins concentrate at the division site in every cell type that has been examined, and in Saccharomyces cerevisiae (Hartwell 1971; Longtine et al. 1996; Lippincott et al. 2001; Dobbelaere and Barral 2004) and at least some Drosophila (Neufeld and Rubin 1994; Adam et al. 2000) and mammalian (Kinoshita et al. 1997; Surka et al. 2002) cell types, the septins are essential for cytokinesis. In S. cerevisiae, the septins are required for formation of the AMR (Bi et al. 1998; Lippincott and Li 1998). However, this cannot be their only role, because the AMR itself is not essential for cytokinesis in this organism (Bi et al. 1998; Korinek et al. 2000; Schmidt et al. 2002). Moreover, there is no evidence that the septins are necessary for AMR formation or function in any other organism. A further complication is that in some cell types, including most Caenorhabditis elegans cells (Nguyen et al. 2000; Maddox et al. 2007) and some Drosophila cells (Adam et al. 2000; Field et al. 2008), the septins do not appear to be essential for cytokinesis even though they localize to the division site.S. pombe has seven septins, four of which (Spn1, Spn2, Spn3, and Spn4) are expressed in vegetative cells and localize to the division site shortly before AMR constriction and septum formation (Longtine et al. 1996; Berlin et al. 2003; Tasto et al. 2003; Wu et al. 2003; An et al. 2004; Petit et al. 2005; Pan et al. 2007; Onishi et al. 2010). Spn1 and Spn4 appear to be the core members of the septin complex (An et al. 2004; McMurray and Thorner 2008), and mutants lacking either of these proteins do not assemble the others at the division site. Assembly of a normal septin ring also depends on the anillin-like protein Mid2, which colocalizes with the septins (Berlin et al. 2003; Tasto et al. 2003). Surprisingly, mutants lacking the septins are viable and form seemingly complete septa with approximately normal timing. These mutants do, however, display a variable delay in separation of the daughter cells, suggesting that the septins play some role(s) in the proper completion of the septum or in subsequent processes necessary for cell separation (Longtine et al. 1996; An et al. 2004; Martín-Cuadrado et al. 2005).It is possible that the septins localize to the division site and yet are nonessential for division in some cell types because their role is redundant with that of some other protein(s) or pathway(s). To explore this possibility in S. pombe, we screened for mutations that were lethal in combination with a lack of septins. The results suggest that the septins cooperate with the AMR during cytokinesis and that, in the absence of septin function, the septum is not formed properly, so that an intact system for recognizing and repairing cell-wall damage becomes critical for cell survival.     

Novel Enzyme Family Found in Filamentous Fungi Catalyzing trans-4-Hydroxylation of l-Pipecolic Acid

Hydroxypipecolic acids are bioactive compounds widely distributed in nature and are valuable building blocks for the organic synthesis of pharmaceuticals. We have found a novel hydroxylating enzyme with activity toward l-pipecolic acid (l-Pip) in a filamentous fungus, Fusarium oxysporum c8D. The enzyme l-Pip trans-4-hydroxylase (Pip4H) of F. oxysporum (FoPip4H) belongs to the Fe(II)/α-ketoglutarate-dependent dioxygenase superfamily, catalyzes the regio- and stereoselective hydroxylation of l-Pip, and produces optically pure trans-4-hydroxy-l-pipecolic acid (trans-4-l-HyPip). Amino acid sequence analysis revealed several fungal enzymes homologous with FoPip4H, and five of these also had l-Pip trans-4-hydroxylation activity. In particular, the homologous Pip4H enzyme derived from Aspergillus nidulans FGSC A4 (AnPip4H) had a broader substrate specificity spectrum than other homologues and reacted with the l and d forms of various cyclic and aliphatic amino acids. Using FoPip4H as a biocatalyst, a system for the preparative-scale production of chiral trans-4-l-HyPip was successfully developed. Thus, we report a fungal family of l-Pip hydroxylases and the enzymatic preparation of trans-4-l-HyPip, a bioactive compound and a constituent of secondary metabolites with useful physiological activities.     

Bacidina genus novum familiaeLecideaceae s. lat. (Ascomycetes lichenisati)

The following eleven species currently classified in the generaBacidia s. lat. andCatillaria s. lat. are transferred to the new genusBacidina Vězda gen. n. (Lecideaceae s. lat.):Bacidina apiahica (Müll. Arg.) comb. n.,B. chloroticula (Nyl.)Vězda etPoelt comb. n.,B. egenula (Nyl.) comb. n.,B. inundata (Fr.) comb. n.,B. mirabilis (Vězda) comb. n.,B. neglecta (Vězda) comb.n.,B. pallidocarnea (Müll. Arg.) comb. n.,B. phacodes (Koerb.) comb.n.,B. scutellifera (Vězda) comb.n.,B. vasakii (Vězda) comb.n., andB. ziamensis (Vězda) comb.n.     

The Sorrow of the Dance Analysts

This discussion criticizes an outdated style of writing about dances and rituals in New Guinea, a difficult task because this area of the world has produced excellent writers on the same subject, notably “Buck” Schieffelin's The Sorrow of the Lonely and the Burning of the Dancers (1976 Schieffelin , Edward 1976 The Sorrow of the Lonely and the Burning of the Dancers . New York : St. Martin's Press . [Google Scholar]). The reasons for failure are given as the review proceeds in the hope that others may gain insights into writing about a difficult and often intractable subject.     

A Novel Link between Fic (Filamentation Induced by cAMP)-mediated Adenylylation/AMPylation and the Unfolded Protein Response

The maintenance of endoplasmic reticulum (ER) homeostasis is a critical aspect of determining cell fate and requires a properly functioning unfolded protein response (UPR). We have discovered a previously unknown role of a post-translational modification termed adenylylation/AMPylation in regulating signal transduction events during UPR induction. A family of enzymes, defined by the presence of a Fic (filamentation induced by cAMP) domain, catalyzes this adenylylation reaction. The human genome encodes a single Fic protein, called HYPE (Huntingtin yeast interacting protein E), with adenylyltransferase activity but unknown physiological target(s). Here, we demonstrate that HYPE localizes to the lumen of the endoplasmic reticulum via its hydrophobic N terminus and adenylylates the ER molecular chaperone, BiP, at Ser-365 and Thr-366. BiP functions as a sentinel for protein misfolding and maintains ER homeostasis. We found that adenylylation enhances BiP''s ATPase activity, which is required for refolding misfolded proteins while coping with ER stress. Accordingly, HYPE expression levels increase upon stress. Furthermore, siRNA-mediated knockdown of HYPE prevents the induction of an unfolded protein response. Thus, we identify HYPE as a new UPR regulator and provide the first functional data for Fic-mediated adenylylation in mammalian signaling.     

Review of the genus Catomus Allard, 1876 (Coleoptera: Tenebrionidae) in Iran

Five species of the genus Catomus Allard, 1876 Allard, E. (1876): Révision des Helopines vrais de Lacordaire. L'Abeille. Journal d'Entomologie, 14, 1–80. [Google Scholar] are known from Iran. Catomus fragilis (Ménétriés, 1848) is recorded from Iran for the first time. Lectotypes of C. persicus Allard, 1876 Allard, E. (1876): Révision des Helopines vrais de Lacordaire. L'Abeille. Journal d'Entomologie, 14, 1–80. [Google Scholar] (type species of the genus) and C. semiruber Allard, 1876 Allard, E. (1876): Révision des Helopines vrais de Lacordaire. L'Abeille. Journal d'Entomologie, 14, 1–80. [Google Scholar] are designated. These species are also redescribed and figured. A key to the species of Catomus in the Iranian fauna is given.     

Somatic embryogenesis of tissue cultures of Papaver somniferum and Papaver orientale and its relationship to alkaloid and lipid metabolism

Transfer from complete to 2,4-D free Gamborg's B5-medium efficiently induced somatic embryogenesis in Papaver tissue cultures (P. somniferum and P. orientale). Embryogenesis was preceded by a strong temporary accumulation of triacylglycerols. In both tissue cultures large amounts of sanguinarine type alkaloids were present, which disappeared during regeneration in the P. orientale cultures but persisted in the P. somniferum cultures. In the P. somniferum cultures protopine and morphine type alkaloids (morphine, codeine, thebaine) appeared about 45 days after exchanging the medium. Thebaine was the main alkaloid in the P. somniferum embryoids accumulating up to 0.2 % of dry weight.