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1.
Kato T Sawada H Yamamoto T Mase K Nakagoshi M 《Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society》2006,19(4):337-345
The larval pigment pattern in the silkworm, Bombyx mori, is formed by melanin, ommochromes and pteridines. During development all these pigments are synthesized autonomously, and possibly also with mutual interaction between them, to yield unique pigment patterns. In order to find the key trigger for such pigment pattern formation, developmental changes in pteridine biosynthesis were studied using the quail mutant (q/q), which has darker larval marks formed by melanin and an abundance of ommochromes in the integument. In the current study, emphasis has been placed on the analysis of GTP-cyclohydrolase I (GTP-CH I), which is a key enzyme for the biosynthesis of pteridines, during the development of the silkworm. Results of Northern blotting showed that in the quail mutant strong signals of GTP-CH I mRNA appeared around each period of ecdysis, while no such signals appeared in the background strain (+q/q) used. Also, both GTP-CH I activities and pteridine content were higher in the quail mutant compared with the background strain. These results strongly suggest that pteridine biosynthesis is closely linked to the formation of melanin and ommochromes. It is also suggested here that in the silkworm a recessive gene (q) may be involved in the regulation of its pigment pattern formation. 相似文献
2.
Eye pigments of normal and mutant types of D. melanogaster have been extracted with water and fractionated by chromatographic adsorption on powdered talc. Spectra of all the fractions obtainable in solution have been measured and the general chemical behavior of the pigments is described. Two chemically distinct groups of pigments are found, to be identified with the earlier designated red and brown components. The red component in the wild-type eye contains three well defined pigments, two of them capable of further subdivision so that the total number of fractions obtained is five. There is also present a brown component pigment which could not be treated quantitatively by the methods employed. All members of the wild-type red component are found in cinnabar eyes, unaccompanied by the brown component. Conversely, brown eyes contain a pigment indistinguishable from the wild-type brown component, virtually alone. In sepia eyes, one red component and two brown component pigments can be distinguished, all three pigments differing from those of wild-type eyes. Pigments apparently identical with those found in wild-type melanogaster eyes have also been found in D. virilis. 相似文献
3.
《Insect Biochemistry》1991,21(7):785-794
In the seasonally diphenic butterfly Araschnia levana14C-labelled tryptophan and 3-hydroxykynurenine, the principal precursors of ommochromes, injected into young pupae caused a pattern specific radiolabel of mature red scales. [14C]glucose and [35S]methionine also labelled red scales but only when injected shortly before or during the time of pigment synthesis in the wing. In developing non-diapause pupae contents of 3-hydroxykynurenine increased until an abrupt decrease when pigments appeared in the wings. In diapausing pupae 3-hydroxykynurenine remained low but increased after injection of 20-hydroxyecdysone which induced pupal-adult development. Supply of wing scale cells with ommochrome precursors via the haemolymph was analysed after injection of [3H]tryptophan. In developing pupae haemolymph contents of [3H]tryptophan and [3H]3-hydroxykynurenine increased at the time of wing pigment formation and decreased shortly before adult emergence. In diapausing pupae haemolymph contents of [3H]tryptophan and [3H]3-hydroxykynurenine were low compared to non-diapause pupae but increased at the time of wing pigment formation after injection of 20-hydroxyecdysone. Isolated wings incubated in Grace's medium containing [14C]tryptophan and [14C]3-hydroxykynurenine incorporated radiolabel specifically into red portions of the wing colour pattern due to labelling of ommatin. Incorporation into red wing areas occurred specifically depending on different colour patterns of the spring- and the summer-morph.The results demonstrate that both tryptophan as well as 3-hydroxykynurenine are delivered via the haemolymph, and both can serve as precursors of ommatin formation in the scale cells. Therefore, the complete set of enzymes for the tryptophan-ommatin pathway is present in scale-forming cells. 相似文献
4.
《Journal of Fermentation and Bioengineering》1993,75(5):353-358
When Arthrobacter hyalinus was grown on isopropanol, a large amount of red pigment was accumulated in the culture broth. The pigment was isolated from the culture broth. With thin layer chromatography, FD mass, IR, 1H-NMR, 13C-NMR, and absorption spectra methods it was found that the red pigments were composed of type III varieties of coproporphyrin, penta carboxyl porphyrin, hexa carboxyl porphyrin, hepta carboxyl porphyrin and uroporphyrin, and some type I uroporphyrin. 相似文献
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6.
《Fungal biology》2023,127(3):927-937
Filamentous fungi are being globally explored for the production of industrially important bioactive compounds including pigments. In the present study, a cold and pH tolerant fungus strain Penicillium sp (GEU_37), isolated from the soil of Indian Himalaya, is characterized for the production of natural pigments as influenced by varying temperature conditions. The fungal strain produces a higher sporulation, exudation, and red diffusible pigment in Potato Dextrose (PD) at 15 °C as compared to 25 °C. In PD broth, a yellow pigment was observed at 25 °C. While measuring the effect of temperature and pH on red pigment production by GEU_37, 15 °C and pH 5, respectively, were observed to be the optimum conditions. Similarly, the effect of exogenous carbon and nitrogen sources and mineral salts on pigment production by GEU_37 was assessed in PD broth. However, no significant enhancement in pigmentation was observed. Chloroform extracted pigment was separated using thin layer chromatography (TLC) and column chromatography. The two separated fractions i.e., fractions I and II with Rf values 0.82 and 0.73, exhibited maximum light absorption, λmax, at 360 nm and 510 nm, respectively. Characterization of pigments using GC–MS showed the presence of the compounds such as phenol, 2,4-bis (1,1-dimethylethyl) and eicosene from fraction I and derivatives of coumarine, friedooleanan, and stigmasterole in fraction II. However, LC-MS analysis detected the presence of derivatives of compound carotenoids from fraction II as well as derivative of chromenone and hydroxyquinoline as major compounds from both the fractions along with other numerous important bioactive compounds. The production of such bioactive pigments under low temperature conditions suggest their strategic role in ecological resilience by the fungal strain and may have biotechnological applications. 相似文献
7.
E. Stratakis 《Journal of insect physiology》1979,25(12):925-929
End products of tryptophan metabolism in Carausius morosus are the ommochromes ommin and xanthommatin in the epidermis, and kynurenic acid in the faeces. During larval and adult life ommochromes and mainly kynurenic acid are formed. The concentration of kynurenic acid in the faeces of adult females is 2.5 times lower than in the larvae and in adult males. Allatectomy on the first day after a larval moult induces a much longer instar (10 days) than normal. After the following moult, the allatectomized animals are transformed into adultoids. The allatectomized and normal larvae produce similar amounts of kynurenic acid and ommochrome during the larval instar. Twenty days after last ecdysis, the ommochrome content in adult and adultoids is increased. In the faeces of adultoids, however, the concentration of kynurenic acid is higher than in normal female adults, but lower than in males and larvae. 相似文献
8.
E. Stratakis 《Journal of insect physiology》1976,22(9):1207-1212
The synthesis of ommochromes, ommin, and xanthommatin, was studied during the embryonic development of Carausius morosus. Ommochrome synthesis begins in the epidermis of the embryo at the 46th day after egg laying. The ommochrome accumulation is directly related to the continuous increase of pigment spots on the epidermis of the pharate larva.The concentration of free tryptophan increases progressively from the germ band formation (15th day), until the final position of the embryo in the egg, with the head immediately beneath the operculum (44th day). Subsequent decrease in concentration of tryptophan coincides with the appearance of ommochrome in the epidermis of the embryo. The tryptophan levels decrease considerably from 44th day till the 60th day. During the stage of the pharate first instar larva tryptophan levels remain low until hatching. The concentrations of the intermediates, kynurenine and 3-HO-kynurenine, show no accumulation and remain low during the whole of ommochrome synthesis. 相似文献
9.
《Journal of Fermentation and Bioengineering》1992,73(1):31-36
Adventitious roots were induced from red beet (Beta vulgaris L. cv. Detroit dark red) by infecting the plant with a soil bacterium, Agrobacterium rhizogenes. Based on analysis of opines which are uniquely produced in transformed hairy roots, the established clone was proved to be a transformed hairy root. In a shake culture of the beet hairy root clone with a liquid medium, it was found that significant amounts of pigments, mainly betanin and vulgaxanthin-I, were released into the medium by the cessation of culture shaking (temporary limitation of oxygen supply). The hairy root cells were capable of propagation even after the cells were subjected to shaking cessation. Repeated-batch culture of the beet hairy root was performed with the cell growth phases for 9 or 10 d and with pigment leakage phases during shaking cessation for 2 d, and more than 20% of the total intracellular pigments were recovered from the culture broth at a culture time of 35 d. The released pigments were confirmed to be substantially identical to those extracted from the hairy root and original plant cells of red beet. 相似文献
10.
Sissel Norgård W.A. Svec Synnöve Liaaen-Jensen A. Jensen R.R.L. Guillard 《Biochemical Systematics and Ecology》1974,2(1):3-6
The chloroplast pigments of one typical representative (Pleurochloris magna) and two potential members (clone BSG Sticho and an isolate called Tunis) of the new class Eustigmatophyceae have been examined by modern methods including mass spectrometry. The three cultures all exhibited the same chloroplast pigments: Chlorophyll a, but no b or c, δ-carotene (I), canthaxanthin (II), violaxanthin (IIIa), and esterified vaucheriaxanthin (IVb) plus some free vaucheriaxanthin (IVa). Furanoid rearrangement of the epoxidic carotenoids complicated the analysis. The unique pigment complement hereby indicated for Eustigmatophyceae is clearly different from the pigment distribution patterns reported for Chlorophyceae and Xanthophyceae. 相似文献
11.
Blood and spleens of phenylhydrazine-injected rats were treated with a solution of acidic methanol and zinc ion to isolate a green pigment. The pigment was resolved into two, I and II, by thin-layer chromatography. Pigment I was a mixture of two isomers of zinc complex of esterified N-phenylprotoporphyrin IX, in which vinyl-substituted pyrrole rings A and B were phenylated; and pigment II was a mixture of two isomers of the porphyrin complex with the N-phenyl group on propionic acid-substituted rings C and D. These pigments were also chemically prepared from the reaction of phenylhydrazine with oxyhemoglobin, independently characterized, and used to confirm the structures of the biological pigments. Determination revealed that the total amount of pigments found in the blood and spleen at 24 h after injection of phenylhydrazine corresponds to about 0.4% of the injected phenylhydrazine. 相似文献
12.
In parallel with the studies reported in the preceding paper(I), the modes of production of characteristic red pigmentsby Chlorella protothecoides cells were investigated under variousculture conditions, (i) During the course of "acetate-bleaching"of algal cells, excretion of red pigments in the medium proceededwith simultaneous disappearance of chlorophyll from algal cells.The total amount (weight) of the red pigments excreted intomedium was slightly less than that of the chlorophyll lost.No red pigment was detectable within the bleaching algal cells.Carotenoids were found to increase or remain nearly constantin their quantities per culture during the process of bleaching,(ii) In a later phase of "glucose-bleaching" some red pigmentswere found to be present inside as well as outside the algalcells, and the excreted pigments underwent further changes turningcolourless, (iii) Both the production of red pigments and disappearanceof chlorophyll were suppressed by light and this light effectwas insensitive to CMU. (iv) During the process of "regreening"of "glucose-bleached" algal cells, no production of red pigmentswas observed either in or outside the algal cells. Based on these results we concluded that the red pigments areproduced from chlorophyll during the bleaching process of algalcells induced by an organic carbon source. (Received July 23, 1968; ) 相似文献
13.
Sunita Singh Moreau Eric Inman Floyd Holmes D. Leonard 《Indian journal of microbiology》2012,52(3):325-331
Culturing the bioluminescent bacterium Photorhabdus luminescens in nutrient broth (NB) is used to recover phase I cells. These phase I cells were highly luminescent for up to 7 h in this media and the luminosity could also be seen with the naked eye after a 15 min eye adjustment period in a dark room. Red pigmentation is a known trait of phase I cells and was visually distinct within the culture media. The color shade of the red pigment varied on nutrient agar and in NB suggesting that the concentration of the pigment produced is dependent upon density of phase I cells within a specified area. The specific growth rate (μ) and doubling time (g) was determined during the logarithmic growth phase to be 0.36 h−1 and 2.1 h, respectively in NB medium. The nematode-bacterium suspension was injected into larvae of Galleria mellonella to test for entomopathogencity. Within 24 h post-injection insect mortality was seen along with dark red pigmentation and extremely high luminosity indicating infection with P. luminescens. 相似文献
14.
Pigmentation and Antibacterial Activity of Fast Neutron- and X-Ray-induced Strains of Monascus purpureus Went 总被引:3,自引:0,他引:3
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Seven new strains of Monascus purpureus Went were induced by neutron and x-ray irradiation. The quantity and quality of pigments produced by these strains differed. Strains N4S and N11S produced twice as much pigment as normal, while another strain, N14S, was albino. An unknown orange pigment was found in young colonies of the N11S strain. This orange pigment reacted with alcohols and malt extract medium to form red pigments. Strains N4S, N11S, X2P, and wild type inhibited the growth of certain bacteria, especially the Bacillus species. Strain N11S had more antibacterial activity than wild type. A major active compound was isolated with an ultraviolet absorption spectrum that was related to those of the red pigments found in this fungus. The active compound(s) was named monascidin. 相似文献
15.
《Insect Biochemistry》1987,17(7):1039-1043
Of a series of derivatives of JH I and methoprene, iodovinylmethoprenol (IVMA) proved most active (ed50s of 3.2 pmol and 20 nM in the Manduca black larval assay and in the prevention of the 20-hydroxyecdysone-induced change to pupal commitment in the epidermis in vitro, respectively). When incubated with nuclei isolated from day 1 fifth instar abdominal epidermis, [125I]IVMA bound specifically to two components with Kds of 4 and 59 nM. This binding was competed by IVMA and methoprene but not by JH I; similar binding by [3H]JH I was not competed by IVMA or methoprene. Specific binding of IVMA was not found in pupally committed epidermis from wandering larvae, but it reappeared in pupal abdominal and thoracic epidermis but not in the wings. Culture of day 2 fifth instar epidermis with 20-hydroxyecdysone to cause pupal commitment caused the loss of the IVMA nuclear binders whereas culture in the absence of hormortes had no effect, indicating that 20HE in the absence of JH downregulates JH receptors. 相似文献
16.
Jost Heinrich Dustmann 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1964,49(1):28-57
Summary From the epidermis of Carausius morosus two ommochromes were isolated, and identified by various means as Xanthommatine and Ommine. Their amount was determined by photometry for animals of different colour.Morphological colour change results mainly from changes in ommochrome content.If the lower part of their compound eyes is blackened, green specimens become brown by an increase of ommochrome production.This colour change can be evoked within a single larval instar.It can also be evoked in adult specimens, although to a small degree only.Dark specimens with unblinded eyes become paler under normal illumination in consequence of an increase of bright pigments and of the increase in bodysize, while the amount of ommochrome increases only very slightly.A decrease of ommochrome content or a loss of ommochrome by faeces or offshed cuticles was never observed.At high temperature (28° C) both, ommochrome production in the epidermis and melanin formation in the cuticle are increased.Implantation of supernumerous Corpora allata causes the ommochrome content to increase. After extirpation of the Corpora allata no decrease of ommochrome content is found but the green pigment, insectoverdin vanishes. Apparently in Carausius ommochrome may deposited but is never removed from the integument, which may explain the similar coloration after both experiments. 相似文献
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18.
Preillumination of excised spinach leaves with red light increases resistance of photosynthetic apparatus to UV radiation 总被引:1,自引:0,他引:1
V. D. Kreslavskii M. S. Khristin N. I. Shabnova V. Yu. Lyubimov 《Russian Journal of Plant Physiology》2012,59(6):717-723
Leaves of spinach (Spinacia oleracea, cv. Ispolinskii) were preilluminated by low-intensity light (1.0 and 1.5 W/m2, 0.5?C3.0 h) with wavelengths ranging from 530 to 730 nm to study the effect of this pretreatment on the activity of photosystem II (PS II), content of photosynthetic pigments, and peroxidase activity in excised leaves exposed to UV-A irradiation. Irradiation of leaves with UV-A suppressed the activity of PS II, reduced the content of chlorophylls (a + b) and carotenoids, and increased the peroxidase activity. Preillumination of leaves with red light (RL, 620?C660 nm) alleviated the inhibitory action of UV-A on PS II activity and reduced the pigment losses but increased the peroxidase activity in leaves and thylakoid membrane preparations, as compared to the respective effects of UV-A light applied without preillumination. The preexposure of leaves to red light alternating with far-red light (FR, 730 nm) removed partly the influence of RL on the parameters under study, which indicates the involvement of phytochrome active form, PFR into stress-induced defense responses in leaves. It is supposed that elevated resistance of photosynthetic apparatus to UV-A radiation was formed with the involvement of PFR and the antioxidant system induced by oxidative stress after preillumination of leaves with red light 相似文献
19.
Sawada H Nakagoshi M Reinhardt RK Ziegler I Koch PB 《Insect biochemistry and molecular biology》2002,32(6):609-615
Color patterns of butterfly wings are composed of single color points represented by each scale. In the case of Precis coenia, at the end of pupal development, different types of pigments are synthesized sequentially in the differently colored scales beginning with white (pterins) followed by red (ommatins) and then black (melanin). In order to explain how formation of these different colors is regulated, we examined the expression of an mRNA-encoding guanosine triphosphate-cyclohydrolase I (GTP-CH I; EC 3.5.4.16), the first key enzyme in the biosynthesis of pteridines, during pigment formation in the wings of P. coenia. The strongest positive signal was recognized around pigment formation one day before butterfly emergence. This GTP-CH I gene expression is paralleled by GTP-CH I enzyme activity measured in wing extracts. We also investigated the effect of 20-hydroxyecdysone on the expression of GTP-CH I mRNA and the enzyme activity during color formation. The results strongly suggest that the onset and duration of the expression of a GTP-CH I mRNA is triggered by a declining ecdysteroid hormone titer during late pupal development. 相似文献
20.
Melanin and novel melanin precursors from Aeromonas media 总被引:2,自引:0,他引:2
Many bacteria produce reddish brown to black pigments and some of these have been characterised. This report describes the isolation and characterisation of a diffusible brown melanin-like pigment from the bacterium Aeromonas media. Physico-chemical testing suggested that the pigment is a true melanin. New butanol-soluble yellow, red and brown pigments were isolated from the A. media strain under reducing conditions during melanogenesis and these pigments were shown to be unstable precursors of the polymeric brown melanin product. 相似文献